Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Vesicle transport protein USE1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g1200 g1200.t11 TSS g1200.t11 8715762 8715762
chr_3 g1200 g1200.t11 isoform g1200.t11 8715821 8716576
chr_3 g1200 g1200.t11 exon g1200.t11.exon1 8715821 8715957
chr_3 g1200 g1200.t11 exon g1200.t11.exon2 8716029 8716576
chr_3 g1200 g1200.t11 cds g1200.t11.CDS1 8716274 8716576
chr_3 g1200 g1200.t11 TTS g1200.t11 8716734 8716734

Sequences

>g1200.t11 Gene=g1200 Length=685
ATGGCAGCAAAACTTGAAATAAATATAAGAAGTTTGTTAAGTACTTGTGAAGAGTTAGTA
AAAGAAGAAGATCAGAAATGGAAATTAACACGATATATAAAAAGCTTAGATTTAATGATC
AACGAATTGTCCGATTAATAAGAATCATATAGCAAAATACCAAGAAAAAGTTCTAGAACT
CAAAACACTTATTAATTATGTTGAACCACAAAAACAGAAAATAGTGAAAACTAGGAGGGA
TGTTGATGAGTCAGAAACAATTTTAAAGGAAATTAAGCAACTCAATAACGCAAAATTCAA
TAAAGAAATCAGAAAAGACTTATTTGATGGTGATGATGCAACAGGATTAAGGAAAAGAGG
GGCAAACGAAAGTTCAGAAAATATGGAAAGATATTATGATAATATTCAAGAAAAATTAGG
AGAAGAAATGCTTGCATTAACTAAAAATCTAAAAGAACAGTATTTAGCTGCCAATAGCAT
AATTAAAAAAGATACAGAGGTCATAACTAAATCCGCAAAAATGGCTCATCAAAATATCGG
ATCACTAGAAAAAGAATCCAAAAAACTAAATGAGCATACAAAACGAGCATGTAAATGTTG
GATGTGGATGATGATTGGTCTCGTAATTATGATATTTATCGCAATGGTCCTATTCATGAA
AATCATGAAGAAAAGGCAATATTAG

>g1200.t11 Gene=g1200 Length=100
MERYYDNIQEKLGEEMLALTKNLKEQYLAANSIIKKDTEVITKSAKMAHQNIGSLEKESK
KLNEHTKRACKCWMWMMIGLVIMIFIAMVLFMKIMKKRQY

Protein features from InterProScan

Transcript Database ID Name Start End E.value
8 g1200.t11 CDD cd15860 SNARE_USE1 5 64 1.4068E-17
2 g1200.t11 PANTHER PTHR13050 USE1-LIKE PROTEIN 7 98 8.1E-24
3 g1200.t11 PANTHER PTHR13050:SF7 VESICLE TRANSPORT PROTEIN USE1 7 98 8.1E-24
1 g1200.t11 Pfam PF09753 Membrane fusion protein Use1 3 95 4.9E-41
6 g1200.t11 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 72 -
7 g1200.t11 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 73 92 -
5 g1200.t11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 93 100 -
4 g1200.t11 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 73 95 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

There are no GO annotations for this transcript.

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values