General information for g12011.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as cGMP-dependent protein kinase, isozyme 1.
Parent gene
- The parent gene g12011 is located on chr_1: 20338960..20347849 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12011 | g12011.t1 | TTS | g12011.t1 | 20338665 | 20338665 |
| chr_1 | g12011 | g12011.t1 | isoform | g12011.t1 | 20338960 | 20347849 |
| chr_1 | g12011 | g12011.t1 | exon | g12011.t1.exon1 | 20338960 | 20339339 |
| chr_1 | g12011 | g12011.t1 | cds | g12011.t1.CDS1 | 20338960 | 20339339 |
| chr_1 | g12011 | g12011.t1 | exon | g12011.t1.exon2 | 20339858 | 20339921 |
| chr_1 | g12011 | g12011.t1 | cds | g12011.t1.CDS2 | 20339858 | 20339921 |
| chr_1 | g12011 | g12011.t1 | exon | g12011.t1.exon3 | 20339989 | 20340057 |
| chr_1 | g12011 | g12011.t1 | cds | g12011.t1.CDS3 | 20339989 | 20340057 |
| chr_1 | g12011 | g12011.t1 | exon | g12011.t1.exon4 | 20340381 | 20340609 |
| chr_1 | g12011 | g12011.t1 | cds | g12011.t1.CDS4 | 20340381 | 20340609 |
| chr_1 | g12011 | g12011.t1 | exon | g12011.t1.exon5 | 20340980 | 20341288 |
| chr_1 | g12011 | g12011.t1 | cds | g12011.t1.CDS5 | 20340980 | 20341288 |
| chr_1 | g12011 | g12011.t1 | exon | g12011.t1.exon6 | 20341413 | 20341615 |
| chr_1 | g12011 | g12011.t1 | cds | g12011.t1.CDS6 | 20341413 | 20341615 |
| chr_1 | g12011 | g12011.t1 | exon | g12011.t1.exon7 | 20341668 | 20341873 |
| chr_1 | g12011 | g12011.t1 | cds | g12011.t1.CDS7 | 20341668 | 20341873 |
| chr_1 | g12011 | g12011.t1 | exon | g12011.t1.exon8 | 20341937 | 20341989 |
| chr_1 | g12011 | g12011.t1 | cds | g12011.t1.CDS8 | 20341937 | 20341989 |
| chr_1 | g12011 | g12011.t1 | exon | g12011.t1.exon9 | 20342049 | 20342279 |
| chr_1 | g12011 | g12011.t1 | cds | g12011.t1.CDS9 | 20342049 | 20342279 |
| chr_1 | g12011 | g12011.t1 | exon | g12011.t1.exon10 | 20342425 | 20342969 |
| chr_1 | g12011 | g12011.t1 | cds | g12011.t1.CDS10 | 20342425 | 20342969 |
| chr_1 | g12011 | g12011.t1 | exon | g12011.t1.exon11 | 20344995 | 20345054 |
| chr_1 | g12011 | g12011.t1 | cds | g12011.t1.CDS11 | 20344995 | 20345054 |
| chr_1 | g12011 | g12011.t1 | exon | g12011.t1.exon12 | 20346902 | 20347131 |
| chr_1 | g12011 | g12011.t1 | cds | g12011.t1.CDS12 | 20346902 | 20347131 |
| chr_1 | g12011 | g12011.t1 | exon | g12011.t1.exon13 | 20347224 | 20347368 |
| chr_1 | g12011 | g12011.t1 | cds | g12011.t1.CDS13 | 20347224 | 20347368 |
| chr_1 | g12011 | g12011.t1 | exon | g12011.t1.exon14 | 20347580 | 20347682 |
| chr_1 | g12011 | g12011.t1 | cds | g12011.t1.CDS14 | 20347580 | 20347682 |
| chr_1 | g12011 | g12011.t1 | exon | g12011.t1.exon15 | 20347737 | 20347849 |
| chr_1 | g12011 | g12011.t1 | cds | g12011.t1.CDS15 | 20347737 | 20347849 |
| chr_1 | g12011 | g12011.t1 | TSS | g12011.t1 | NA | NA |
Sequences
>g12011.t1 Gene=g12011 Length=2940
ATGGAAAATATGAAAGTACGAATAGCTGGAACATTAAAGTCCATTAAAGAAGTCGATCCA
GTTATAATAAAAGAATGGGAAGTAGCAGTCGCTAAAGAAAATGCCAAAGAAAGTGCGAGC
GGGCGAAATGCCAAAGAACGTTGGAAATTATTTAAAAACATTTCACGATTTGGAATATTG
CAAAAGAATGCCACGGAAGAGGACAATTTATCTTCACGCAGTTCACTTTATCAACCTTTC
AAACGCACCTCAGTTAACTCATTTGGATCTCGCTTATGGGCTGAATTACCATTGCCAATT
GATGAATGTCACGACAACGATGATGATTTCGTTTTTTTAATTTTATGTAGAAAGCGTGGA
AAATTTAATGAAAATAACGAAAAAGTTGATGAAAAATTAAAGTCAGATGAAGAGCGCAAG
TATGGCGAAATCCCAGCAAGAATTTATTTGTTTTATTTGAAAGCATGTGGCATGGTCACA
TTAGTTTTATTCTTCACAAGTACATTATTATGGCAAGCATTGAAAGTCCTCACTGATGTC
TGGCTAAGAGAATGGACAGACAATGACAATTCTACAACAACTTCATCTGATCGCGAAGTT
GGATCATACGATCTATCGATATTAAATATTGATGGTGTCTACCCAAAAAATACATATCGT
GAGTTTGATCGTTCATACTCAAAGTCATCATTAAATCACAATCAAAACAACAATATTGAT
AGCTCGAAAAATGAACGCAGTGAATCGGGATTTGATTCAATCACTGATGGAATATTTAAT
AGTAACAATCAAGAATTCACACACATTCCATTAAATATTCCGTTGCCACCAACACGCGCT
CCACCGCGACCGCCAGTTGAGAATGATTTCTCACCGCAACAATCGCCAATACCATGCCGC
AAGCATCATCAACAACCGATACCTAATATCAATTTTATTGAATCATCGCCAACTCTCACA
CCCATGGGAAATTATGAAGATGATTTCAATATGAAAAGTATTCGTTTAATTGAAAGCATC
GATAATATCGAGGGAAGTGAAGTAGGAAGAAGAACTGAAAGTGAAATTAAAATTGATGAA
TTGAATGATAAGCTGACGTCAGATGTGAATGTTAGAAAGGAGGGCTTCATATCAGAAAAA
ATAAAACATGAAGAATTGGAGGAATTGGAAATTAAAGTTTATCCAAAGGACAGAATAACC
GAAAAAATGATAACATCTGCCATAGTCTCAAATGACTTCTTATGCAACATTTTGGATAAC
GAGTCGATAAAAACTGTCGTGAATGCTATGCGACTTGAATCGTATGATGAAAATAGCTTC
ATTGTAACTGAGGGTGATTCGGGAAATTTTCTTTACGTATCCGCTGAGGGAATATTTGAG
GTGATCAAAAATGGCAAACCTATTAAATCTTTTGGACCGGCAGTTGTTTTTGGAGAGCTT
TCAATTTTATATAAAGCAAAGCGTTTTGCGTCAATTAAAGCCTTGACGCTATGCAAGGTC
TTCTCGCTCGAACGAAAAGTTTTTCAGAAAATTATGATGAGTTGTGGACGTCGTGAACGT
GAGGAGAATATCAAATTCCTTCAATCCGTACCAGTACTAAAAGACTTGCCATTGATGGTA
CTCAACAAGATATCTGATCTGCTGAAACGTGAATATTTTCCATCGGGCTCTACGATCATA
AGACAAGGCGATAGTGGCGAGAAATTTTATATCATAAAAAGTGGAAGCGTGAATGTGATT
AAGACCGATGATAATAATGGACAACGTCTTGTTGGCATCTTGAAGCGAGGCGCATATTTT
GGAGAGCAGGCACTGATACATCAAGATAAACGTCTAGCAAGCGTTATTGCCAATTCACCC
GGAACTGATTGCTTGACACTCGACCGAAATGCATTCGATGCTTATCTTGGTGGTTTAGAG
AAAATTCGCAATACAAAAGTTGTTGATAAAAACTTCCAACAAGTCCAAAAAGTTTCTGCA
AAGAGTGAATATGCTAATATTCAATTATCAGATCTAAAGTTTATTGGAACTCTTGGTGTT
GGTGGATTTGGCAGAGTTGAACTGGTGCAATACAAAAATAAGACGACATTTGCTCTGAAA
TGTCTCAAGAAAATTGAAATGCTTCAGCAGCAGCAACAGGAACATGCATATAATGAGAAA
GCAATTTTATTTGAATGCAACAGTCCGTTTATTGTTAGATTATATAAAACATTCAAGGAT
AAAAAGTATCTATATTATTTAATGGAGCCTTGTCTGGGTGGCGATGTTTGGACGGTGTTA
CAAAAATATAAGTTTTTCGATGAGAAAATAGCAAAATTTATTACTGCGTGTGTGGTATTG
GCATTGGAATATCTTCACTCAAAGAACATCATTTATAGAGATTTGAAGCCAGAAAATTTG
CATGACTCAAATGGATATATAAAGTTGGTCGATTTTGGTTTTGCTAAAAGCTTGCGACCT
GGTTCTAAAACATTCACATTTGCAGGCACACCGGAATACGTTGCACCGGAAATTATACTA
AATAAAGGCCACGATCAAAGTGTGGATTGTTGGAGTTTAGGTATTTTAATTCATGAGCTC
CTTATTGGAAAACCACCATTTCGTGCAAAAGAGCAAATGCAAACATACAATCTCATTTTA
CGTGGAATCGATTGTGTTCAGTTGCCTCAAAAAATTCCCAAAAAAGCACAAATGTTAATT
AAAAGACTGTGTCGTCAAACGGGATCAGAACGAATTGGAGTACAGAAAAATGGCATAAGA
GATATAAAAGCACATCAATGGTTTACTGGCTTTCAATGGGATAAACTTGAGGCGCGTAAA
ATGACAGCTCCATTGATTATGCCTGTGAAGAGCAACACAGATATGAGCAATTTTGATGAT
TATCCGACAGACTATGATGAACCACCTGATGATTTGAGTGGATGGGATGATGATTTTTAA
>g12011.t1 Gene=g12011 Length=979
MENMKVRIAGTLKSIKEVDPVIIKEWEVAVAKENAKESASGRNAKERWKLFKNISRFGIL
QKNATEEDNLSSRSSLYQPFKRTSVNSFGSRLWAELPLPIDECHDNDDDFVFLILCRKRG
KFNENNEKVDEKLKSDEERKYGEIPARIYLFYLKACGMVTLVLFFTSTLLWQALKVLTDV
WLREWTDNDNSTTTSSDREVGSYDLSILNIDGVYPKNTYREFDRSYSKSSLNHNQNNNID
SSKNERSESGFDSITDGIFNSNNQEFTHIPLNIPLPPTRAPPRPPVENDFSPQQSPIPCR
KHHQQPIPNINFIESSPTLTPMGNYEDDFNMKSIRLIESIDNIEGSEVGRRTESEIKIDE
LNDKLTSDVNVRKEGFISEKIKHEELEELEIKVYPKDRITEKMITSAIVSNDFLCNILDN
ESIKTVVNAMRLESYDENSFIVTEGDSGNFLYVSAEGIFEVIKNGKPIKSFGPAVVFGEL
SILYKAKRFASIKALTLCKVFSLERKVFQKIMMSCGRREREENIKFLQSVPVLKDLPLMV
LNKISDLLKREYFPSGSTIIRQGDSGEKFYIIKSGSVNVIKTDDNNGQRLVGILKRGAYF
GEQALIHQDKRLASVIANSPGTDCLTLDRNAFDAYLGGLEKIRNTKVVDKNFQQVQKVSA
KSEYANIQLSDLKFIGTLGVGGFGRVELVQYKNKTTFALKCLKKIEMLQQQQQEHAYNEK
AILFECNSPFIVRLYKTFKDKKYLYYLMEPCLGGDVWTVLQKYKFFDEKIAKFITACVVL
ALEYLHSKNIIYRDLKPENLHDSNGYIKLVDFGFAKSLRPGSKTFTFAGTPEYVAPEIIL
NKGHDQSVDCWSLGILIHELLIGKPPFRAKEQMQTYNLILRGIDCVQLPQKIPKKAQMLI
KRLCRQTGSERIGVQKNGIRDIKAHQWFTGFQWDKLEARKMTAPLIMPVKSNTDMSNFDD
YPTDYDEPPDDLSGWDDDF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 21 | g12011.t1 | CDD | cd00038 | CAP_ED | 414 | 521 | 8.19016E-19 |
| 20 | g12011.t1 | CDD | cd00038 | CAP_ED | 532 | 637 | 1.10777E-26 |
| 22 | g12011.t1 | CDD | cd05572 | STKc_cGK | 678 | 935 | 3.09757E-141 |
| 16 | g12011.t1 | Gene3D | G3DSA:2.60.120.10 | Jelly Rolls | 381 | 518 | 9.5E-33 |
| 15 | g12011.t1 | Gene3D | G3DSA:2.60.120.10 | Jelly Rolls | 519 | 647 | 2.7E-37 |
| 13 | g12011.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 669 | 960 | 1.8E-103 |
| 14 | g12011.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 755 | 953 | 1.8E-103 |
| 30 | g12011.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 227 | 247 | - |
| 31 | g12011.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 228 | 244 | - |
| 4 | g12011.t1 | PANTHER | PTHR24353:SF58 | LP05330P | 161 | 979 | 1.2E-297 |
| 5 | g12011.t1 | PANTHER | PTHR24353 | CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE | 161 | 979 | 1.2E-297 |
| 8 | g12011.t1 | PRINTS | PR00103 | cAMP-dependent protein kinase signature | 553 | 567 | 8.3E-7 |
| 7 | g12011.t1 | PRINTS | PR00103 | cAMP-dependent protein kinase signature | 568 | 582 | 8.3E-7 |
| 9 | g12011.t1 | PRINTS | PR00103 | cAMP-dependent protein kinase signature | 598 | 607 | 8.3E-7 |
| 6 | g12011.t1 | PRINTS | PR00103 | cAMP-dependent protein kinase signature | 610 | 621 | 8.3E-7 |
| 2 | g12011.t1 | Pfam | PF00027 | Cyclic nucleotide-binding domain | 433 | 514 | 6.1E-13 |
| 3 | g12011.t1 | Pfam | PF00027 | Cyclic nucleotide-binding domain | 551 | 635 | 7.3E-20 |
| 1 | g12011.t1 | Pfam | PF00069 | Protein kinase domain | 675 | 928 | 1.5E-63 |
| 17 | g12011.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 148 | - |
| 19 | g12011.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 149 | 171 | - |
| 18 | g12011.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 172 | 979 | - |
| 28 | g12011.t1 | ProSitePatterns | PS00888 | Cyclic nucleotide-binding domain signature 1. | 559 | 575 | - |
| 29 | g12011.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 678 | 700 | - |
| 34 | g12011.t1 | ProSiteProfiles | PS50042 | cAMP/cGMP binding motif profile. | 414 | 529 | 27.442 |
| 33 | g12011.t1 | ProSiteProfiles | PS50042 | cAMP/cGMP binding motif profile. | 532 | 644 | 30.1 |
| 35 | g12011.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 672 | 928 | 41.969 |
| 32 | g12011.t1 | ProSiteProfiles | PS51285 | AGC-kinase C-terminal domain profile. | 929 | 979 | 11.26 |
| 26 | g12011.t1 | SMART | SM00100 | cnmp_10 | 414 | 527 | 1.0E-14 |
| 25 | g12011.t1 | SMART | SM00100 | cnmp_10 | 532 | 654 | 1.5E-21 |
| 27 | g12011.t1 | SMART | SM00220 | serkin_6 | 672 | 928 | 9.8E-78 |
| 24 | g12011.t1 | SMART | SM00133 | pkinase_C_6 | 929 | 979 | 6.8E-5 |
| 11 | g12011.t1 | SUPERFAMILY | SSF51206 | cAMP-binding domain-like | 361 | 514 | 1.26E-22 |
| 10 | g12011.t1 | SUPERFAMILY | SSF51206 | cAMP-binding domain-like | 520 | 637 | 1.09E-30 |
| 12 | g12011.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 662 | 968 | 2.77E-81 |
| 23 | g12011.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 149 | 171 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004674 | protein serine/threonine kinase activity | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
| GO:0004692 | cGMP-dependent protein kinase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K07376 (cGMP-dependent protein kinase 1)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed