General information for g12054.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Glutathione hydrolase 1 proenzyme.
Parent gene
- The parent gene g12054 is located on chr_1: 20676680..20679505 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12054 | g12054.t1 | isoform | g12054.t1 | 20672265 | 20676858 |
| chr_1 | g12054 | g12054.t1 | exon | g12054.t1.exon1 | 20672265 | 20673840 |
| chr_1 | g12054 | g12054.t1 | cds | g12054.t1.CDS1 | 20672265 | 20673840 |
| chr_1 | g12054 | g12054.t1 | exon | g12054.t1.exon2 | 20676043 | 20676243 |
| chr_1 | g12054 | g12054.t1 | cds | g12054.t1.CDS2 | 20676043 | 20676243 |
| chr_1 | g12054 | g12054.t1 | exon | g12054.t1.exon3 | 20676680 | 20676858 |
| chr_1 | g12054 | g12054.t1 | cds | g12054.t1.CDS3 | 20676680 | 20676858 |
| chr_1 | g12054 | g12054.t1 | TSS | g12054.t1 | 20677273 | 20677273 |
| chr_1 | g12054 | g12054.t1 | TTS | g12054.t1 | NA | NA |
Sequences
>g12054.t1 Gene=g12054 Length=1956
ATGGTGTTGAGAGTTTTTGGCATAACAAAAGATTATTGGGTATGGATTTTCCCACTTAGT
GGCTTCACTCTCGGTTGGTGGCTTGATAAAAAAGAAACAGAAAGAATGATTACATATAGA
GATCGATCCGCTTTATATGGACGAGATACAACTGCTCCAGACTACAAACCAAGTTGGCCG
AAAATTTCCGGCATGTTAATCTTTCTCGCTGTTGTGATATTTTTGCTTATTACTCATGAA
GAGTCGGACCCTAATATAATAAAAAATGGAAGAGACATTCCTGATCCAGAAAAACCTTTG
CCGCCATCATCATCGAAGCTTTATAAATTCAAGCATGCTGCTATATCTACAGATTCCCAA
GAATGTAGTGATATTGCAAGAAAATTGATGGAACGTCATGCAAATGGATTTGATTTGGCT
TTAAATTCTTTAATTTGTATGAGTCTAACAAATATTCAATCATGTGGGCTTTTTGGTGGA
TTCTTATTAACAGCATACATTCCATCAGAACAAAAATCAATTGTAATTGATGCTCAAATG
ACATCTCCGAAAAACTTTCATTTCACACCTAAACAAGAAGGTGAAGACTATTTGAAAGTT
AAAGAGGGCCCAATGAGCATTGCAAATTCAGGATTTCTGAAAGGATTATGGGAAATTCAT
AGAAATTATAGTAAAATGTCTTGGCATGATATTATTGACCCAGTAATTTTATTGTGCAGA
GAAGGCATTGTATTAACTAAACATCTTCGTGATAGCATTGAGATGAATAGCCATATATTG
AATGATACTTATTTTCGTAATATTTTTATTGACAGCAGCTCAGGGAGAATCAAAAGAGTT
GGATCAAAAGTTGTTTTGTTAAAGCAATGTGATTTCTTAGAATTATTAAGTAAACAAAAT
ACCACAGAGGACATTTTTTCTGGAGATGTGGGAAAATTATTAGCTGACGACCTAAAAACA
GCAAATGCTATTGTAGATATAAATGATCTCCAAACATACAAAGTAAAAGTTTATGAAGCG
AGAAAATTTGATTTAATAAATGACAACGTCCTTCTTGTGCCTAATACATGTGCAATATTA
ATTCCGGCAATTTTAAATATTTTAATGAAATATAATTTCAATTCAACATGGTTTGATAGT
CAACACAATACTAATTTATCAATTTTAATGCATCATCGAATAATTGAGACTTTTAAGCAT
GTGTTTGCAATGAGATCTGAAATGAGTGATTCAGATTTCATGGATACTGAAAATATCATT
AATTATCTACTTTCAAATGATTTTGCCTATAATGTAATAAAATCTATTAATGACTCGTTT
GTAAAGCCTTATCTACAGGATTACAATTTATCAACAATTGTTCCACAAAATCATGGAACA
TCTCACATTTCGATAGTTGCTGAAAATAATGACTTGATATCTGTTACAAGCTCAATAAAT
TTTTATTTTGGCTCTGGAATTATTGGCTCTAAATCGGGTTTAATTTTTAATAATGGTATG
GATGACTTTTCTTATTTTAATCGATCTGTAAATTACTTTGGATTGAAAGAGACACCCACT
AATTTCCCTGAACCTCAAAAACGAGCGTTATCGTCAGTTGCTCCTCTTATAATAATTAAT
AAAAATAATGAACGGTCTAATCTAATCATTGGTGCTGCTGGAGGCTCAAAAATAATTACA
TCAATTTCAATGGCAATTCTAAGATTTTTATATGATTCAACTGATCTCAAAGAAATAATT
GATGCACCAAGATTTCATCATCAATTAATTCCAAACATTTTAGAATATGAATATGGAAAT
TTAATGTGGATTATCAATGGACTTAATGAGAAAGGACATAATTCTAGTCGATATCGATAT
AGAGGTACTATCATAAATGCACTGAAAATTGATCAACGAAAAATTCATGCGATATCTGAT
TATAGAAAGGATTCAAGTGGAGTAGCTGGATTTTAA
>g12054.t1 Gene=g12054 Length=651
MVLRVFGITKDYWVWIFPLSGFTLGWWLDKKETERMITYRDRSALYGRDTTAPDYKPSWP
KISGMLIFLAVVIFLLITHEESDPNIIKNGRDIPDPEKPLPPSSSKLYKFKHAAISTDSQ
ECSDIARKLMERHANGFDLALNSLICMSLTNIQSCGLFGGFLLTAYIPSEQKSIVIDAQM
TSPKNFHFTPKQEGEDYLKVKEGPMSIANSGFLKGLWEIHRNYSKMSWHDIIDPVILLCR
EGIVLTKHLRDSIEMNSHILNDTYFRNIFIDSSSGRIKRVGSKVVLLKQCDFLELLSKQN
TTEDIFSGDVGKLLADDLKTANAIVDINDLQTYKVKVYEARKFDLINDNVLLVPNTCAIL
IPAILNILMKYNFNSTWFDSQHNTNLSILMHHRIIETFKHVFAMRSEMSDSDFMDTENII
NYLLSNDFAYNVIKSINDSFVKPYLQDYNLSTIVPQNHGTSHISIVAENNDLISVTSSIN
FYFGSGIIGSKSGLIFNNGMDDFSYFNRSVNYFGLKETPTNFPEPQKRALSSVAPLIIIN
KNNERSNLIIGAAGGSKIITSISMAILRFLYDSTDLKEIIDAPRFHHQLIPNILEYEYGN
LMWIINGLNEKGHNSSRYRYRGTIINALKIDQRKIHAISDYRKDSSGVAGF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 19 | g12054.t1 | Gene3D | G3DSA:1.10.246.130 | - | 346 | 451 | 3.6E-17 |
| 18 | g12054.t1 | Gene3D | G3DSA:3.60.20.40 | - | 454 | 651 | 2.9E-56 |
| 3 | g12054.t1 | PANTHER | PTHR11686:SF9 | RE13973P | 62 | 645 | 7.5E-133 |
| 4 | g12054.t1 | PANTHER | PTHR11686 | GAMMA GLUTAMYL TRANSPEPTIDASE | 62 | 645 | 7.5E-133 |
| 7 | g12054.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 135 | 160 | 6.9E-45 |
| 9 | g12054.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 206 | 224 | 6.9E-45 |
| 10 | g12054.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 224 | 243 | 6.9E-45 |
| 8 | g12054.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 324 | 340 | 6.9E-45 |
| 12 | g12054.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 352 | 371 | 6.9E-45 |
| 13 | g12054.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 460 | 478 | 6.9E-45 |
| 5 | g12054.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 484 | 502 | 6.9E-45 |
| 6 | g12054.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 525 | 540 | 6.9E-45 |
| 11 | g12054.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 551 | 568 | 6.9E-45 |
| 1 | g12054.t1 | Pfam | PF08040 | MNLL subunit | 7 | 59 | 1.1E-12 |
| 2 | g12054.t1 | Pfam | PF01019 | Gamma-glutamyltranspeptidase | 130 | 644 | 1.5E-104 |
| 21 | g12054.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 11 | - |
| 24 | g12054.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 12 | 28 | - |
| 20 | g12054.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 29 | 61 | - |
| 23 | g12054.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 62 | 79 | - |
| 22 | g12054.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 80 | 651 | - |
| 17 | g12054.t1 | SUPERFAMILY | SSF56235 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) | 105 | 644 | 5.02E-97 |
| 15 | g12054.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 12 | 29 | - |
| 16 | g12054.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 62 | 79 | - |
| 14 | g12054.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 350 | 369 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003954 | NADH dehydrogenase activity | MF |
| GO:0006751 | glutathione catabolic process | BP |
| GO:0036374 | glutathione hydrolase activity | MF |
| GO:0005739 | mitochondrion | CC |
KEGG
Orthology
- This transcript has been annotated with K18592 (gamma-glutamyltranspeptidase / glutathione hydrolase / leukotriene-C4 hydrolase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
