General information for g12123.t7
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Peroxisomal multifunctional enzyme type 2.
Parent gene
- The parent gene g12123 is located on chr_1: 21153582..21154814 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12123 | g12123.t7 | TSS | g12123.t7 | 21150894 | 21150894 |
| chr_1 | g12123 | g12123.t7 | isoform | g12123.t7 | 21150983 | 21154814 |
| chr_1 | g12123 | g12123.t7 | exon | g12123.t7.exon1 | 21150983 | 21150989 |
| chr_1 | g12123 | g12123.t7 | exon | g12123.t7.exon2 | 21151266 | 21151566 |
| chr_1 | g12123 | g12123.t7 | exon | g12123.t7.exon3 | 21151623 | 21151872 |
| chr_1 | g12123 | g12123.t7 | exon | g12123.t7.exon4 | 21152021 | 21152605 |
| chr_1 | g12123 | g12123.t7 | cds | g12123.t7.CDS1 | 21152057 | 21152605 |
| chr_1 | g12123 | g12123.t7 | exon | g12123.t7.exon5 | 21152703 | 21152826 |
| chr_1 | g12123 | g12123.t7 | cds | g12123.t7.CDS2 | 21152703 | 21152826 |
| chr_1 | g12123 | g12123.t7 | exon | g12123.t7.exon6 | 21152896 | 21153096 |
| chr_1 | g12123 | g12123.t7 | cds | g12123.t7.CDS3 | 21152896 | 21153096 |
| chr_1 | g12123 | g12123.t7 | exon | g12123.t7.exon7 | 21153157 | 21153426 |
| chr_1 | g12123 | g12123.t7 | cds | g12123.t7.CDS4 | 21153157 | 21153426 |
| chr_1 | g12123 | g12123.t7 | exon | g12123.t7.exon8 | 21153593 | 21153799 |
| chr_1 | g12123 | g12123.t7 | cds | g12123.t7.CDS5 | 21153593 | 21153799 |
| chr_1 | g12123 | g12123.t7 | exon | g12123.t7.exon9 | 21154256 | 21154814 |
| chr_1 | g12123 | g12123.t7 | cds | g12123.t7.CDS6 | 21154256 | 21154467 |
| chr_1 | g12123 | g12123.t7 | TTS | g12123.t7 | 21154817 | 21154817 |
Sequences
>g12123.t7 Gene=g12123 Length=2504
ACGAAAGTCCTTCTGCAATCATGGTTTTGAGATTTGACAATCGTGTTGTTGTTGTAACTG
GTGCAGGTGCTGGTTTAGGTAGAGAATATGCACTTTTATTTGGCAAGCGTGGTGCTAAAG
TGGTTGTGAACGATCTTGGTGGCAATTTTCATGGTGAAGGAAACTCATCTAGAGCTGCAG
ACTTGGTTGTAGAAGAAATTAGAAAACTTGGAGGAACAGCAGTTGCTGATTACAATAGTG
TCGTCGAAGGACATAAAATTATTGAAACTGCAATTTCAAATTATGGACGCATTGATGTGC
TTGTTAATAATGCCGGAATTCTTCGTGATAAATCAATTGCCAACATCTCGGATAATGACT
GGAATTTAATTCATGATGTTCACTTAAAGGGAAGCTTCATGACAACTCGTGCTGCTTGGC
CATACTTTAAAAAACAAAAGTTCGGTCGTATTATAATGACCACCAGTAATAGTGGAATTT
ATGGAAATTTCGGTCAAGCTAACTATTCAGCAGCAAAGCTCGGTTTAATTGGATTAGCTA
ACACAGTTGCAATTGAAGTATGATAATATTAATTTTTAAATAGCTGCATCAAGAATGACA
AAAGGTATTTTACCTGATGATCTATTTGATGAAATGAAACCAGAATTGATTGCACCAGTA
GTGGTTTACTTATGTCATGAAGACACAATGGATAATGGTCAAGTCATTGAGAGTGCAGTT
GGTTTTGCAACAAAAGTTCATTTTGTACGAGGCAAGGGAAGTTTAATTCGAGATAGCATC
AAAGATGTTCCAACACCTGAAACTGTTAAGGCGAAATGGGGCAAAATTACTGACATGAAG
AATGCAAAGCATTTCAATTCAAATATGGAAGTCAGTTCTTCATTTGTCAGCGTTATGGAT
AGATTGAAAAATCCCTTGCCTAAAGATACATTTGAAGATACTTACACTTATACATTCCGT
GACACAATTCTATATGCATTGGGAATTGGAGTTGGAACCGAAGATGAAAGCAATTTGAAA
TTCATTTATGAAAATCATCCAAACTTTGCCGCATTCCCAACTTATGCAGTTATTCCTTCT
ATTATTATGCTAATGACATCAAGCATAACACCATCAGCCCTGGAAAACTTTGATCTTTCT
CAAGTCTTGCATGGCGAACAATTTATTGAAATTGTAAATCCTATGAGAACAGAAGGAACA
CTTACAACTCGTGGAAATGTGATTGATGTTCAACAGAAAAAATCTGGTGTTGTTGTTACT
ACTGAATGTCTTTCATATGATGAAGGCGGTCAACTTATGACACGATCACAGTCAAGTACA
TTCATTGTACGCGGAAAATCAAACGGAAAATATGGTGGAAATCCAGAAAAAGTGATCAAC
ATTATACCAAATCCAAGCAGACAACCTGATCACACTTCGATTGTCAAGACGAATGTTGAT
CAAGCTGCTTTATATCGTCTTTCAGGCGATCTCAATCCATTGCACATCGATCCAAATTTT
GCACTTCTTGGTGGTTTTAATCGTCCAATTATTCACGGTTTGTGCACTTTCGGTGTCTCT
GTTCGTGCAGTGTTGAAACAATATGCTGATAATGACCCATCACTTTTCAAGGCTGTTAAA
GTTCGTTTTACTAAACCTGTTTTTCCTGGTGATACCTTGAAAGTTGAGATGTGGCAAGAA
GGCAACAGAATTCATTTTAGAACTCTTCTCGCTGAGTCTAACACTGAAGTTATTAGTGGC
GCTTATGTGGATTTATTGAACGTTATAAAATCGACATCAACATCAGAAAGCATTCCACAG
TCATCTAGCAACATGGCACTTCAATCGGACGCAGTTTTTGCAGCAATTAAAGAACGAGTC
AATGCTGATAAAGCAAAGGCAAAAAGTGTTAATGGAGTTTTTCTTTATAAAATTACAAAG
GATGGAAAAGTTGCAAAAGAATGGACATTGGATTTGAAAAATGCAGTTATATCAGAAGGT
GCACCATCAGGAAAAGCCGATACAACAATCACTATTGCTGATGAAGACTTTATTGATATT
GCCCTTGGTAAATTAAATCCACAACAAGCATTCATGAAGGGAAAACTAAAGATTCAGGGA
AATATTATGCTGGCACAAAAACTCAGCCCACTACTCAAGACAGATGCTAAATTGTAAAGC
GGATAGTAACAAAAATACACAATTATTTTGTTCATATGACTGCGTGTGTCAATCAACGAC
TCTTTTCATTATTTTTTTGTCATTCTGCTGGGCAATATATGTATAATTATAGACACAAAT
ATCGCTTTAATTGCAATTATATTCCAAAATGAAATTTTAGCTCATAAGCAATTTTTTACT
TCTTATCAACTTATTTCTTTCCAATGGTGGTCAAGAAAAATTAATTTAATGTTTTGAGAA
GAAATTAAAATTAAATTGACATTCATTTTTTTAAATGTGTTTTGTATAATGCTAATTTGA
TGAGGGATTTTATAATAAAATGAAAACTGGGGCTTATAAGAAAA
>g12123.t7 Gene=g12123 Length=520
MTKGILPDDLFDEMKPELIAPVVVYLCHEDTMDNGQVIESAVGFATKVHFVRGKGSLIRD
SIKDVPTPETVKAKWGKITDMKNAKHFNSNMEVSSSFVSVMDRLKNPLPKDTFEDTYTYT
FRDTILYALGIGVGTEDESNLKFIYENHPNFAAFPTYAVIPSIIMLMTSSITPSALENFD
LSQVLHGEQFIEIVNPMRTEGTLTTRGNVIDVQQKKSGVVVTTECLSYDEGGQLMTRSQS
STFIVRGKSNGKYGGNPEKVINIIPNPSRQPDHTSIVKTNVDQAALYRLSGDLNPLHIDP
NFALLGGFNRPIIHGLCTFGVSVRAVLKQYADNDPSLFKAVKVRFTKPVFPGDTLKVEMW
QEGNRIHFRTLLAESNTEVISGAYVDLLNVIKSTSTSESIPQSSSNMALQSDAVFAAIKE
RVNADKAKAKSVNGVFLYKITKDGKVAKEWTLDLKNAVISEGAPSGKADTTITIADEDFI
DIALGKLNPQQAFMKGKLKIQGNIMLAQKLSPLLKTDAKL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g12123.t7 | CDD | cd03448 | HDE_HSD | 267 | 387 | 0.00000 |
| 11 | g12123.t7 | Gene3D | G3DSA:3.30.160.260 | - | 54 | 107 | 0.00000 |
| 10 | g12123.t7 | Gene3D | G3DSA:3.10.129.10 | Hotdog Thioesterase | 110 | 396 | 0.00000 |
| 9 | g12123.t7 | Gene3D | G3DSA:3.30.1050.10 | - | 402 | 517 | 0.00000 |
| 4 | g12123.t7 | PANTHER | PTHR13078:SF56 | PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE 2 | 1 | 390 | 0.00000 |
| 5 | g12123.t7 | PANTHER | PTHR13078 | PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE 2-RELATED | 1 | 390 | 0.00000 |
| 1 | g12123.t7 | Pfam | PF13452 | N-terminal half of MaoC dehydratase | 170 | 228 | 0.00017 |
| 3 | g12123.t7 | Pfam | PF01575 | MaoC like domain | 267 | 381 | 0.00000 |
| 2 | g12123.t7 | Pfam | PF02036 | SCP-2 sterol transfer family | 417 | 515 | 0.00000 |
| 7 | g12123.t7 | SUPERFAMILY | SSF54637 | Thioesterase/thiol ester dehydrase-isomerase | 114 | 252 | 0.00000 |
| 6 | g12123.t7 | SUPERFAMILY | SSF54637 | Thioesterase/thiol ester dehydrase-isomerase | 267 | 384 | 0.00000 |
| 8 | g12123.t7 | SUPERFAMILY | SSF55718 | SCP-like | 407 | 515 | 0.00000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
There are no GO annotations for this transcript.
KEGG
Orthology
- This transcript has been annotated with K12405 ((3R)-3-hydroxyacyl-CoA dehydrogenase / 3a,7a,12a-trihydroxy-5b-cholest-24-enoyl-CoA hydratase / enoyl-CoA hydratase 2 [EC:1.1.1.- 4.2.1.107 4.2.1.119])
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
