General information for g12126.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Mitogen-activated protein kinase kinase kinase kinase 3.
Parent gene
- The parent gene g12126 is located on chr_1: 21184495..21185582 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12126 | g12126.t1 | TTS | g12126.t1 | 21181500 | 21181500 |
| chr_1 | g12126 | g12126.t1 | isoform | g12126.t1 | 21181928 | 21185582 |
| chr_1 | g12126 | g12126.t1 | exon | g12126.t1.exon1 | 21181928 | 21182036 |
| chr_1 | g12126 | g12126.t1 | cds | g12126.t1.CDS1 | 21181928 | 21182036 |
| chr_1 | g12126 | g12126.t1 | exon | g12126.t1.exon2 | 21182098 | 21182227 |
| chr_1 | g12126 | g12126.t1 | cds | g12126.t1.CDS2 | 21182098 | 21182227 |
| chr_1 | g12126 | g12126.t1 | exon | g12126.t1.exon3 | 21182285 | 21182414 |
| chr_1 | g12126 | g12126.t1 | cds | g12126.t1.CDS3 | 21182285 | 21182414 |
| chr_1 | g12126 | g12126.t1 | exon | g12126.t1.exon4 | 21182474 | 21182583 |
| chr_1 | g12126 | g12126.t1 | cds | g12126.t1.CDS4 | 21182474 | 21182583 |
| chr_1 | g12126 | g12126.t1 | exon | g12126.t1.exon5 | 21182661 | 21182846 |
| chr_1 | g12126 | g12126.t1 | cds | g12126.t1.CDS5 | 21182661 | 21182846 |
| chr_1 | g12126 | g12126.t1 | exon | g12126.t1.exon6 | 21182996 | 21183051 |
| chr_1 | g12126 | g12126.t1 | cds | g12126.t1.CDS6 | 21182996 | 21183051 |
| chr_1 | g12126 | g12126.t1 | exon | g12126.t1.exon7 | 21183117 | 21183541 |
| chr_1 | g12126 | g12126.t1 | cds | g12126.t1.CDS7 | 21183117 | 21183541 |
| chr_1 | g12126 | g12126.t1 | exon | g12126.t1.exon8 | 21183620 | 21184435 |
| chr_1 | g12126 | g12126.t1 | cds | g12126.t1.CDS8 | 21183620 | 21184435 |
| chr_1 | g12126 | g12126.t1 | exon | g12126.t1.exon9 | 21184495 | 21184526 |
| chr_1 | g12126 | g12126.t1 | cds | g12126.t1.CDS9 | 21184495 | 21184526 |
| chr_1 | g12126 | g12126.t1 | exon | g12126.t1.exon10 | 21184595 | 21184688 |
| chr_1 | g12126 | g12126.t1 | cds | g12126.t1.CDS10 | 21184595 | 21184688 |
| chr_1 | g12126 | g12126.t1 | exon | g12126.t1.exon11 | 21184795 | 21185089 |
| chr_1 | g12126 | g12126.t1 | cds | g12126.t1.CDS11 | 21184795 | 21185089 |
| chr_1 | g12126 | g12126.t1 | exon | g12126.t1.exon12 | 21185272 | 21185399 |
| chr_1 | g12126 | g12126.t1 | cds | g12126.t1.CDS12 | 21185272 | 21185399 |
| chr_1 | g12126 | g12126.t1 | exon | g12126.t1.exon13 | 21185496 | 21185582 |
| chr_1 | g12126 | g12126.t1 | cds | g12126.t1.CDS13 | 21185496 | 21185582 |
| chr_1 | g12126 | g12126.t1 | TSS | g12126.t1 | 21185641 | 21185641 |
Sequences
>g12126.t1 Gene=g12126 Length=2598
ATGCAGCCGATATACAAAAGTAATCCGCATGAAGATTACGAGTTGTTGCAGAAAATAGGC
TCAGGCACTTATGGGAGTGTGTATAAAGGAAGGAATGTAAAAACGGGCGAATATGCAGCT
ATTAAAGTCATAAAAATAGAAACGGGCGATGACTTTCAACAGATCGAACAAGAAATCACC
ATGCTCAAGGGCTGCAATCATAAAAACATCATAAGTTACTATGGCTCATATCTTCGTCGA
GATAAATTGTGGATATGCATGGAATTGTGTGCGTACAGCTTGCAAGATATTTATCAAGTC
ATTGGACCACTGACAGAACAGCAAATAGCATACGTGTGTCGAGAAACGTTACTTGGTTTA
CAATATTTGCATAATCGAGGAAAGATGCATAGAGATATCAAGGGAGCCAATATTTTGTTG
ACTGACAATGGTGATGTAAAATTAGCTGATTTTGGAGTGTCTGCACAAATCACTGCAACA
ATTAATAAGCGGAAAAGTTTTATTGGAACTCCATATTGGATGAGTCCAGAAGTTGCTGCA
GTTGAAAGAAAAGGAGGATATAATCATTTGTGTGATATTTGGGCGGTTGGGATTACTGCT
ATTGAACTTGCTGAGCTTCAACCCCCGATGTTTGAGATGCATCCAATGAGAGTTTTATTT
CTTATGTCTAAAAGCAACTTTAAACCTCCTACGTTAAAGGAGAAAGAGCTGTGGTCACCT
ATATTTCATTCCTTTGTTAAAATCGCACTTACGAAAAATCCGAAAAAGCGTCCAGATTCT
GAGCGATTACTTCAGCATCCGTTTGTTAATGGTGGTGGTGCTGGAACGGGCGAAATGTCG
GTTCGTTTGATGCGAGAACTTTTGCACAAATATCAAAATCCTCACAATTACAATGATCAT
GAGATTGACGATGAAGGTGCTGTAGGCATTTCTTCTGTTCCAAAACGCATTCCAAGTAAA
ATCGGGACTGTAGAAAGAGTTCATCATATGCATGGAGAAAGCTCAAAAATGATGGTTACC
AATAATAATAATAACAATAACAATATAAGTTGTAGTGACTCACAACTCTCTCCACCTGAT
ACATTACCCAGCGAAATGAGTCTATCAAGTTATATGGATGCTCAATGGGATCGAATGACA
TTGTCTCATAATCGAGGCTGTAATCGTACACCTGACGAAGGTCCGAGTAATAATGAACCG
AAACCATCTACTTCTGGAATAAATGCAGATTCACCTAAGCGACATAATTCAATGGATCGA
CTCGTAGGAATGTTTAATGATTTAGGATCAGCAGTTAATCGTCAGAGAAGTTTAAGTGAC
AGTGAACAGAATCAACCAGACATTCTAAATAATCTCAACAATACTCCTCCCGTGCCTCCA
AAGAGAAAACATCGTCATCGATCTCCACCATTCCATCATCCCATCACAAACGGTCTTCCT
CCTACGCCAAAGGTACTGATGGGAGCGTGTTTCTCTAAAGTATTTAATGGTTGCCCTTTG
CATATCAACTGTACAGCTTCTTGGATTAATCCGGAAACTCGCGACCAGCATATTCTGATT
GGTGCTGATGAGGGAATATTTACATTAAATATGAATGAGATACATGATGCAGTTATAGAT
CAAATATATCCACGAAAAACAATATGGCTTTACGTAATTAAGGATGTATTGATGAGTTTG
TCGGGCAAATCGAGTCAATTATACCGCCACGATTTAGTTGCGCTGCATTCCAAACAAGCG
ACAAGTCGATTTACCCTGCAGCTTCATATGCAAAAAATTCCAGAAAAGTTGGTTCCCAGA
AAATTCGCTCTCACTACAAAAGTTCCCGATACAAAAGGATGCTTTCAGTGTTGCGTTATA
CGGAATCCTTATAATGGTTATAAATATTTAGCGGGTAGACTTTCATCTGGTATATTTCTC
ATGCAGTGGTATGATCCATTGAATAAGTTTATGTTATTGAAGCAATGTGAATGGCCTGCT
CACAGTTTGCCTATGAATGATAATTCAGTATTTGAAATGATAATTTTGCCAGAATTGGAA
TATCCGCTAGTGTGTGTGGGAGTCTCGAAATCATACGGAGGCAACGGCTTGAAACTTGAA
TTGTTCAATACAAATAGTGCTGCTGGCTGGTTCAGTTTGTCATATGATGACGATGATTTT
TCAGATATGGATAGAACTGCAACAATGATTCCAAAACGACTTCCAATGAAAATTATTAAA
GTGCATCAGATGGAAAAAGATATCATTGCAATCGTGCACGATAATGTTATAGAAATGGTA
GATTTAAGTGGACAACCAAAAACTTCTAAGAAAATTGGTCTCTCAAAATCTGAGTTTGGC
TTTAAAGTTGAAGGAATGGTGATAGTTCAAAATGATAGCATTTTGGCATTTCATTCTCAC
GGAATGCAAGGAAGATCTCTTAGAAATGGTGCCATTACGCAGGAAATTACAGATACGAGT
AAAGTTTATCGATTATTAGGAAGTGAAAAGGTTGTGTGTCTCGAGAGTAGTCAAAAAAGA
AGCAATAATAATGATGGAAGTGCATCAGAAGAGTTTCACGATCTGTACACTTTGGCTGGT
CATGAAGCTAGTTATTAG
>g12126.t1 Gene=g12126 Length=865
MQPIYKSNPHEDYELLQKIGSGTYGSVYKGRNVKTGEYAAIKVIKIETGDDFQQIEQEIT
MLKGCNHKNIISYYGSYLRRDKLWICMELCAYSLQDIYQVIGPLTEQQIAYVCRETLLGL
QYLHNRGKMHRDIKGANILLTDNGDVKLADFGVSAQITATINKRKSFIGTPYWMSPEVAA
VERKGGYNHLCDIWAVGITAIELAELQPPMFEMHPMRVLFLMSKSNFKPPTLKEKELWSP
IFHSFVKIALTKNPKKRPDSERLLQHPFVNGGGAGTGEMSVRLMRELLHKYQNPHNYNDH
EIDDEGAVGISSVPKRIPSKIGTVERVHHMHGESSKMMVTNNNNNNNNISCSDSQLSPPD
TLPSEMSLSSYMDAQWDRMTLSHNRGCNRTPDEGPSNNEPKPSTSGINADSPKRHNSMDR
LVGMFNDLGSAVNRQRSLSDSEQNQPDILNNLNNTPPVPPKRKHRHRSPPFHHPITNGLP
PTPKVLMGACFSKVFNGCPLHINCTASWINPETRDQHILIGADEGIFTLNMNEIHDAVID
QIYPRKTIWLYVIKDVLMSLSGKSSQLYRHDLVALHSKQATSRFTLQLHMQKIPEKLVPR
KFALTTKVPDTKGCFQCCVIRNPYNGYKYLAGRLSSGIFLMQWYDPLNKFMLLKQCEWPA
HSLPMNDNSVFEMIILPELEYPLVCVGVSKSYGGNGLKLELFNTNSAAGWFSLSYDDDDF
SDMDRTATMIPKRLPMKIIKVHQMEKDIIAIVHDNVIEMVDLSGQPKTSKKIGLSKSEFG
FKVEGMVIVQNDSILAFHSHGMQGRSLRNGAITQEITDTSKVYRLLGSEKVVCLESSQKR
SNNNDGSASEEFHDLYTLAGHEASY
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g12126.t1 | CDD | cd06613 | STKc_MAP4K3_like | 12 | 269 | 0.0 |
| 6 | g12126.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 9 | 315 | 4.2E-108 |
| 13 | g12126.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 388 | 414 | - |
| 14 | g12126.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 388 | 412 | - |
| 12 | g12126.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 434 | 475 | - |
| 15 | g12126.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 434 | 457 | - |
| 3 | g12126.t1 | PANTHER | PTHR48012:SF18 | HAPPYHOUR, ISOFORM A | 4 | 845 | 0.0 |
| 4 | g12126.t1 | PANTHER | PTHR48012 | STERILE20-LIKE KINASE, ISOFORM B-RELATED | 4 | 845 | 0.0 |
| 7 | g12126.t1 | PIRSF | PIRSF038172 | MAPKKKK | 1 | 865 | 8.2E-302 |
| 2 | g12126.t1 | Pfam | PF00069 | Protein kinase domain | 13 | 269 | 4.0E-60 |
| 1 | g12126.t1 | Pfam | PF00780 | CNH domain | 510 | 825 | 4.0E-47 |
| 11 | g12126.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 19 | 42 | - |
| 17 | g12126.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 13 | 269 | 46.251 |
| 16 | g12126.t1 | ProSiteProfiles | PS50219 | Citron homology (CNH) domain profile. | 499 | 831 | 19.332 |
| 10 | g12126.t1 | SMART | SM00220 | serkin_6 | 13 | 269 | 4.0E-91 |
| 9 | g12126.t1 | SMART | SM00036 | nik_2 | 504 | 838 | 5.7E-65 |
| 5 | g12126.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 10 | 270 | 2.32E-83 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008349 | MAP kinase kinase kinase kinase activity | MF |
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K08833 (mitogen-activated protein kinase kinase kinase kinase 5)
Pathway
This gene does not belong to any pathways.
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
