General information for g12128.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Adenosine receptor A2a.
Parent gene
- The parent gene g12128 is located on chr_1: 21199171..21201145 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12128 | g12128.t1 | isoform | g12128.t1 | 21198032 | 21201145 |
| chr_1 | g12128 | g12128.t1 | exon | g12128.t1.exon1 | 21198032 | 21198486 |
| chr_1 | g12128 | g12128.t1 | cds | g12128.t1.CDS1 | 21198032 | 21198486 |
| chr_1 | g12128 | g12128.t1 | exon | g12128.t1.exon2 | 21199167 | 21199234 |
| chr_1 | g12128 | g12128.t1 | cds | g12128.t1.CDS2 | 21199167 | 21199234 |
| chr_1 | g12128 | g12128.t1 | exon | g12128.t1.exon3 | 21199314 | 21199537 |
| chr_1 | g12128 | g12128.t1 | cds | g12128.t1.CDS3 | 21199314 | 21199537 |
| chr_1 | g12128 | g12128.t1 | exon | g12128.t1.exon4 | 21199949 | 21201145 |
| chr_1 | g12128 | g12128.t1 | cds | g12128.t1.CDS4 | 21199949 | 21201145 |
| chr_1 | g12128 | g12128.t1 | TSS | g12128.t1 | NA | NA |
| chr_1 | g12128 | g12128.t1 | TTS | g12128.t1 | NA | NA |
Sequences
>g12128.t1 Gene=g12128 Length=1944
ATGGTAACAAAAGTGGCAAATGCTGACACATCGATGGGAATAACTGAAATAATGTTGGCA
AATAGTGATGAAAATAACACTATGATACCCGATGCTTATAGCACGCCCATGGTCAAAGAT
GAATTTGTTATTTGGGCTATACTCGATGGTATTCTTCTCGTGGCAATTTTATGTGGCAAT
ATTCTCACAATTTTAGCAGTCCGTTATAGTCGAAGGCTAAGGAGTGTAATTTCAAATCAA
TTTGTACTTTCATTAGCCATTTCTGATATTCTTGTTGGATTGACTTTGCCTTATCACTTG
GCATTCTATCTTGATACGCAGCTCGGTGAGAGCAAACATTTTTGTTTATTGCGCTTCTTC
CTTGTGATAATGGCCTGTTGCGTCTCGATATGGAATTTGATTGCCATCGCTCTTGATCGA
TATATAGCAATTTGCTATCCACTACACTATAGCAGATATATGACACGCAAAATTGCCCTC
TGTTTGATGGCGAGCGGTTGGATTGTTGGCTTTATTATTGGTGGCATCCCATTAATTTGG
AATAAATGGGAATACGCACAAGGATGCGAATTTGATCAGATATTTTACCCATGGTATATG
GTTGGTGTTATTACGCCAGTATTTTCTATGGTCTGGCTCACCATGTCCTTCGTATACTGT
AGAATATGGCGTGAAGCATCGAAGCAAGTAAAACAGCTTCGAGTGACAGGCCAACAAGAA
GGAACATCAGATTGGAAATCTGTACAGATGGTGCTATTGATTCTTGGATGTTTTACGATC
TGTTGGCTACCGTATTTTATCGTAGCATGCTCACAAATTTATAAAATCACTGAGAATAGC
TCAGTTGTTGCATATATGGCAGCATTTACTCTTGCAATGTCAAATTCATTTTTAAATCCA
ATAATTTACGCTTGGAAGAATTCAAATTTTCGTCAAGCATTTATAAATTTACTAAAATGT
AAAAGTCCTGATACACTAGAACCAAGTCAAAGTATGCGATCGAACTTACATAGAAAAAGC
TCATCAGCACAACACCAAGACACGCTATCAGGTGCTTTTCCTAATTACTCAACACCTCCC
TTCATGAAGAAAATTGAACCTATTAATGCAATGGGAATTACGTTCGAGGAAGATGAGGAT
AAAATTTCAGCATACGAATCATCATCACAATCGACCTCAAATACCGCAAAGCCAAAACAA
TTATCTACCACAAATCCAGCTGCTGTAACTATTAAAATAGAATCTGATACGACAAAGAAT
TCAATTATCATTTCTACTACAACCCTACCATCACTTCAACAAGACATTGATGATGACAAT
CGTTCTCATCCTATCATTACTTCCACTTCATCATCTTTGCCTTCTTCTTCTCATCTTCCA
TCACCACCATCATTAGAAATCAAGCAACAAATTTCATTAGAACAAAACTCAACATCACTA
CCACAACAAAAGCATCAAACAATTGGAATTGATAATGGAACAATGAAAACGGGAAATCTA
ATTGTAAATAAATTAATTGAAAACTATGGCTACGATAATAAAGACATTGTCGATGATGAA
GGCATTATGAGGAAGTACAAAGAGAATTTTCTTAATGTCAATGACTGTTCAATTGTCACC
GTAATGAATGAAAGCTCCAAGAGAAAATCAAAGTCTGCAAACAGCATAATTGTCACTAAA
CAACCAAACAAGAGTCAGAGTCATGGTTCTATCTATAATCACGATGTTTGCAAGTCAAAT
ATTGCAGTCGATTCGAATAGCGTTAGAGCAGAAAAAACATCACAGTCTAATAAAAATCTT
TTTCCAACATTTAATTTTGGTCGAAAGTATATATCGAAAAGTTTTAATAGTGCCGTTGAT
GGGTTAAATAACAAACATCAGCAGCACTCAAATAGTACAGAAAAATTTTACCCTAAAAGC
ACCGAACTCAATTTTCCCGCTTAA
>g12128.t1 Gene=g12128 Length=647
MVTKVANADTSMGITEIMLANSDENNTMIPDAYSTPMVKDEFVIWAILDGILLVAILCGN
ILTILAVRYSRRLRSVISNQFVLSLAISDILVGLTLPYHLAFYLDTQLGESKHFCLLRFF
LVIMACCVSIWNLIAIALDRYIAICYPLHYSRYMTRKIALCLMASGWIVGFIIGGIPLIW
NKWEYAQGCEFDQIFYPWYMVGVITPVFSMVWLTMSFVYCRIWREASKQVKQLRVTGQQE
GTSDWKSVQMVLLILGCFTICWLPYFIVACSQIYKITENSSVVAYMAAFTLAMSNSFLNP
IIYAWKNSNFRQAFINLLKCKSPDTLEPSQSMRSNLHRKSSSAQHQDTLSGAFPNYSTPP
FMKKIEPINAMGITFEEDEDKISAYESSSQSTSNTAKPKQLSTTNPAAVTIKIESDTTKN
SIIISTTTLPSLQQDIDDDNRSHPIITSTSSSLPSSSHLPSPPSLEIKQQISLEQNSTSL
PQQKHQTIGIDNGTMKTGNLIVNKLIENYGYDNKDIVDDEGIMRKYKENFLNVNDCSIVT
VMNESSKRKSKSANSIIVTKQPNKSQSHGSIYNHDVCKSNIAVDSNSVRAEKTSQSNKNL
FPTFNFGRKYISKSFNSAVDGLNNKHQQHSNSTEKFYPKSTELNFPA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 26 | g12128.t1 | CDD | cd00637 | 7tm_classA_rhodopsin-like | 45 | 307 | 6.87032E-69 |
| 10 | g12128.t1 | Gene3D | G3DSA:1.20.1070.10 | - | 23 | 327 | 1.6E-69 |
| 37 | g12128.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 329 | 349 | - |
| 38 | g12128.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 383 | 405 | - |
| 40 | g12128.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 385 | 405 | - |
| 36 | g12128.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 624 | 647 | - |
| 39 | g12128.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 624 | 639 | - |
| 2 | g12128.t1 | PANTHER | PTHR24247:SF234 | AGAP000369-PA | 21 | 318 | 4.6E-107 |
| 3 | g12128.t1 | PANTHER | PTHR24247 | 5-HYDROXYTRYPTAMINE RECEPTOR | 21 | 318 | 4.6E-107 |
| 8 | g12128.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 44 | 68 | 7.1E-33 |
| 4 | g12128.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 78 | 99 | 7.1E-33 |
| 6 | g12128.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 122 | 144 | 7.1E-33 |
| 5 | g12128.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 247 | 271 | 7.1E-33 |
| 7 | g12128.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 285 | 311 | 7.1E-33 |
| 1 | g12128.t1 | Pfam | PF00001 | 7 transmembrane receptor (rhodopsin family) | 59 | 303 | 1.3E-47 |
| 18 | g12128.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 41 | - |
| 22 | g12128.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 42 | 69 | - |
| 14 | g12128.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 70 | 80 | - |
| 21 | g12128.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 81 | 104 | - |
| 16 | g12128.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 105 | 115 | - |
| 24 | g12128.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 116 | 138 | - |
| 13 | g12128.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 139 | 157 | - |
| 25 | g12128.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 158 | 180 | - |
| 17 | g12128.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 181 | 199 | - |
| 20 | g12128.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 200 | 222 | - |
| 12 | g12128.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 223 | 250 | - |
| 19 | g12128.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 251 | 276 | - |
| 15 | g12128.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 277 | 281 | - |
| 23 | g12128.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 282 | 305 | - |
| 11 | g12128.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 306 | 647 | - |
| 35 | g12128.t1 | ProSitePatterns | PS00237 | G-protein coupled receptors family 1 signature. | 128 | 144 | - |
| 41 | g12128.t1 | ProSiteProfiles | PS50262 | G-protein coupled receptors family 1 profile. | 59 | 303 | 37.985 |
| 34 | g12128.t1 | SMART | SM01381 | 7TM_GPCR_Srsx_2 | 53 | 318 | 0.0059 |
| 9 | g12128.t1 | SUPERFAMILY | SSF81321 | Family A G protein-coupled receptor-like | 33 | 340 | 1.46E-62 |
| 30 | g12128.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 42 | 64 | - |
| 29 | g12128.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 81 | 103 | - |
| 32 | g12128.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 116 | 138 | - |
| 33 | g12128.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 158 | 180 | - |
| 27 | g12128.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 195 | 217 | - |
| 31 | g12128.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 251 | 273 | - |
| 28 | g12128.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 283 | 305 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0007186 | G protein-coupled receptor signaling pathway | BP |
| GO:0016021 | integral component of membrane | CC |
| GO:0004930 | G protein-coupled receptor activity | MF |
KEGG
Orthology
- This transcript has been annotated with K04134 (muscarinic acetylcholine receptor)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed