General information for g12133.t59
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Adenosylhomocysteinase.
Parent gene
- The parent gene g12133 is located on chr_1: 21225692..21227548 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12133 | g12133.t59 | TTS | g12133.t59 | 21223774 | 21223774 |
| chr_1 | g12133 | g12133.t59 | isoform | g12133.t59 | 21223873 | 21225692 |
| chr_1 | g12133 | g12133.t59 | exon | g12133.t59.exon1 | 21223873 | 21224414 |
| chr_1 | g12133 | g12133.t59 | cds | g12133.t59.CDS1 | 21223873 | 21224414 |
| chr_1 | g12133 | g12133.t59 | exon | g12133.t59.exon2 | 21224471 | 21224873 |
| chr_1 | g12133 | g12133.t59 | cds | g12133.t59.CDS2 | 21224471 | 21224873 |
| chr_1 | g12133 | g12133.t59 | exon | g12133.t59.exon3 | 21225261 | 21225451 |
| chr_1 | g12133 | g12133.t59 | cds | g12133.t59.CDS3 | 21225261 | 21225451 |
| chr_1 | g12133 | g12133.t59 | exon | g12133.t59.exon4 | 21225668 | 21225692 |
| chr_1 | g12133 | g12133.t59 | cds | g12133.t59.CDS4 | 21225668 | 21225692 |
| chr_1 | g12133 | g12133.t59 | TSS | g12133.t59 | 21225730 | 21225730 |
Sequences
>g12133.t59 Gene=g12133 Length=1161
ATGTCTAAGCCGGCCTATAAAGTTGCTGATATAAGTCTTGCAGACCTCGGTCGCAAAGAA
ATCATCATTGCTGAAAATGAAATGCCCGGTTTGATGGCAATTCGTAAAAAGTATGGGCCA
CAAAAGATTTTGAAGGGAGCTCGCATTGCCGGCTGCTTGCATATGACAGTACAAACTGCA
GTTCTTATTGAAACACTCTTGGAATTGGGTGCTGAGTTTCCAAATAACGAGCCTCTTAAT
ATGATTTTGGATGACGGCGGTGACTTAACAAATCTTGTTCACGAAAAATATCCACAATAC
TTGACTGGAATCAAGGGAATCAGCGAGGAGACAACGACTGGAGTTCACAATCTTTACAAC
ATGTTCAGGGAAAATAAACTTAAAGTTCCTGCAATCAATGTAAATGATTCTGTTACCAAG
AGCAAATTCGATAATCTCTATGGATGCCGTGAATCTTTAATTGATGGAATTAAGCGTGCT
ACTGATGTCATGATTGCAGGAAAAGTGTGTGTTGTTGCTGGTTATGGAGATGTTGGTAAA
GGTTGTGCTCAAGCTCTTCGATCATTTGGCGGCAGAGTTATTATTACAGAAATTGATCCA
ATCAATGCTCTGCAAGCAGCAATGGAAGGATATGAAGTAACAACTATGGATGAAGCAGCC
AGTGAAGGAAATATTTTTGTTACAACAACTGGTTGCACAGACATCATTATGGGAGATCAT
TTTATGAAAATGAAAGATGATTCAATTGTTTGTAATATTGGTCATTTTGATTGTGAAGTT
AACGTAGCTTGGCTTGAAGAAAATGCAAAGGAAAAAGTCAATATTAAGCCACAAGTTGAT
AGATTTGTTTTAAGTAACGGCAATCACATTATTCTTTTGGCTGAAGGACGATTGGTTAAT
CTCGGTTGTGCCAAGGGCCATCCATCGTTTGTGATGTCAAATTCTTTCTGTAACCAGACA
CTTGCCCAAATTGAGCTTTGGACTAAAGCTGAAAAATATCCAGTTGGAGTTCATATGTTA
CCTAAGAAACTTGATGAGGAAGTCGCAGCACTTCATCTTGAAAAATTGGGAGTAAAACTT
ACAAAACTCACAGAAAAGCAAGCTAAATATTTGGGACTTTGTGCAGAAGGACCATTCAAA
CCTGAATACTATCGCTACTAA
>g12133.t59 Gene=g12133 Length=386
MSKPAYKVADISLADLGRKEIIIAENEMPGLMAIRKKYGPQKILKGARIAGCLHMTVQTA
VLIETLLELGAEFPNNEPLNMILDDGGDLTNLVHEKYPQYLTGIKGISEETTTGVHNLYN
MFRENKLKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVCVVAGYGDVGK
GCAQALRSFGGRVIITEIDPINALQAAMEGYEVTTMDEAASEGNIFVTTTGCTDIIMGDH
FMKMKDDSIVCNIGHFDCEVNVAWLEENAKEKVNIKPQVDRFVLSNGNHIILLAEGRLVN
LGCAKGHPSFVMSNSFCNQTLAQIELWTKAEKYPVGVHMLPKKLDEEVAALHLEKLGVKL
TKLTEKQAKYLGLCAEGPFKPEYYRY
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g12133.t59 | CDD | cd00401 | SAHH | 15 | 373 | 0.0 |
| 13 | g12133.t59 | Gene3D | G3DSA:3.40.50.1480 | - | 1 | 73 | 8.6E-31 |
| 12 | g12133.t59 | Gene3D | G3DSA:3.40.50.1480 | - | 74 | 150 | 4.0E-33 |
| 10 | g12133.t59 | Gene3D | G3DSA:3.40.50.720 | - | 151 | 302 | 3.1E-75 |
| 11 | g12133.t59 | Gene3D | G3DSA:3.40.50.1480 | - | 303 | 386 | 4.0E-40 |
| 4 | g12133.t59 | PANTHER | PTHR23420 | ADENOSYLHOMOCYSTEINASE | 5 | 73 | 3.4E-208 |
| 6 | g12133.t59 | PANTHER | PTHR23420:SF20 | ADENOSYLHOMOCYSTEINASE | 5 | 73 | 3.4E-208 |
| 5 | g12133.t59 | PANTHER | PTHR23420 | ADENOSYLHOMOCYSTEINASE | 76 | 386 | 3.4E-208 |
| 7 | g12133.t59 | PANTHER | PTHR23420:SF20 | ADENOSYLHOMOCYSTEINASE | 76 | 386 | 3.4E-208 |
| 14 | g12133.t59 | PIRSF | PIRSF001109 | SAHH | 1 | 76 | 9.3E-25 |
| 15 | g12133.t59 | PIRSF | PIRSF001109 | SAHH | 73 | 386 | 3.8E-144 |
| 1 | g12133.t59 | Pfam | PF05221 | S-adenosyl-L-homocysteine hydrolase | 6 | 72 | 1.1E-31 |
| 2 | g12133.t59 | Pfam | PF05221 | S-adenosyl-L-homocysteine hydrolase | 95 | 385 | 4.6E-62 |
| 3 | g12133.t59 | Pfam | PF00670 | S-adenosyl-L-homocysteine hydrolase, NAD binding domain | 145 | 306 | 2.9E-85 |
| 17 | g12133.t59 | ProSitePatterns | PS00739 | S-adenosyl-L-homocysteine hydrolase signature 2. | 167 | 183 | - |
| 19 | g12133.t59 | SMART | SM00996 | AdoHcyase_2 | 5 | 385 | 9.4E-250 |
| 18 | g12133.t59 | SMART | SM00997 | AdoHcyase_NAD_2 | 145 | 306 | 1.5E-113 |
| 9 | g12133.t59 | SUPERFAMILY | SSF52283 | Formate/glycerate dehydrogenase catalytic domain-like | 3 | 386 | 7.94E-91 |
| 8 | g12133.t59 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 145 | 306 | 4.2E-77 |
| 20 | g12133.t59 | TIGRFAM | TIGR00936 | ahcY: adenosylhomocysteinase | 77 | 378 | 2.7E-150 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004013 | adenosylhomocysteinase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
