General information for g12195.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable isoaspartyl peptidase/L-asparaginase GA20639.
Parent gene
- The parent gene g12195 is located on chr_1: 21684937..21686260 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12195 | g12195.t1 | TSS | g12195.t1 | 21684910 | 21684910 |
| chr_1 | g12195 | g12195.t1 | isoform | g12195.t1 | 21684937 | 21686260 |
| chr_1 | g12195 | g12195.t1 | exon | g12195.t1.exon1 | 21684937 | 21685018 |
| chr_1 | g12195 | g12195.t1 | cds | g12195.t1.CDS1 | 21684937 | 21685018 |
| chr_1 | g12195 | g12195.t1 | exon | g12195.t1.exon2 | 21685078 | 21685103 |
| chr_1 | g12195 | g12195.t1 | cds | g12195.t1.CDS2 | 21685078 | 21685103 |
| chr_1 | g12195 | g12195.t1 | exon | g12195.t1.exon3 | 21685161 | 21685662 |
| chr_1 | g12195 | g12195.t1 | cds | g12195.t1.CDS3 | 21685161 | 21685662 |
| chr_1 | g12195 | g12195.t1 | exon | g12195.t1.exon4 | 21685714 | 21685818 |
| chr_1 | g12195 | g12195.t1 | cds | g12195.t1.CDS4 | 21685714 | 21685818 |
| chr_1 | g12195 | g12195.t1 | exon | g12195.t1.exon5 | 21685965 | 21686260 |
| chr_1 | g12195 | g12195.t1 | cds | g12195.t1.CDS5 | 21685965 | 21686260 |
| chr_1 | g12195 | g12195.t1 | TTS | g12195.t1 | 21686317 | 21686317 |
Sequences
>g12195.t1 Gene=g12195 Length=1011
ATGAGGTTTTTGACATTAATTTCTCTTTTGACACTTTTTAGTTATAGCTTTGGCGGTAAT
GTGACAATTATTTGTCATGGAGGCGCTGGCTCTGTTGCCCCTTCTAGATTTATAGGAAAA
TACCGTGGCACTGTTCTTGCAGTTCAAATTGGTTATAACATTTTAAAAACTAATGGTACT
TCTCTTGATGCTGTTGAGGCAGCTGTGCGAAGTATGGAAGTCGATGAAGAATTTAATGCT
GGTTATGGAAGTGTTTTGACAAGTGAAGGTAAAGTTGAAATGGATGCTTGCATCATGGAC
GGAGAAACAATGAAGGTTGGCGCAGTGACAGGAGTTCAAAATATTTTTCATACAATTACT
CTTGCAAGAAAAGTTATGGAAAAAACGAGATACAACTTTTTAAGCCCAGAAGGGGCAATG
AAGCTTGCAAAAGATGAAGGATTTCAATTTTTACCTGAAGGAACTTTAGTAACAGAGCGT
GCAAAACTTTCATTAGAAAATTGGAAACGAAATCAAAATCAAAGTTTAGGCGAGCAAGTT
GGCGAGGGTGGGACTGTAGGCTGTGCTGCCTACGATATCAATGGCAATTTAGCAGCAGCA
ACATCAACAGGTGGCCTAACTGGAAAAATGAGTGGAAGAATCGGTGATACACCGATAGTT
GGAGGAGGCACTTATGCGGACAATAAATTGGGAGCACTTTCTGCAACAGGCACTGGTGAA
GTCATAATGAAATCGGTACTTGTATATGACATTTTAAAACGATACGAATACAAGCAAATA
AGTTTGCAACAAGCAGCACAAGAAGCATGTGATGATATGACTGAAAGATATGATGATGAT
GGGGGTGTTATTGGCCTCGACAAAGACGGTAATGTCGCAATTGGATTCTCTTCTCCTCAA
ATGTCTTGGGCTTATCAAAATGAACCAGAAAATGTTCATTATGGAATTGAACATGAAATT
TCTCACATTTATAATATTGCAGAATGTAAGAGTAAAAATTGTTTCCAATAA
>g12195.t1 Gene=g12195 Length=336
MRFLTLISLLTLFSYSFGGNVTIICHGGAGSVAPSRFIGKYRGTVLAVQIGYNILKTNGT
SLDAVEAAVRSMEVDEEFNAGYGSVLTSEGKVEMDACIMDGETMKVGAVTGVQNIFHTIT
LARKVMEKTRYNFLSPEGAMKLAKDEGFQFLPEGTLVTERAKLSLENWKRNQNQSLGEQV
GEGGTVGCAAYDINGNLAAATSTGGLTGKMSGRIGDTPIVGGGTYADNKLGALSATGTGE
VIMKSVLVYDILKRYEYKQISLQQAAQEACDDMTERYDDDGGVIGLDKDGNVAIGFSSPQ
MSWAYQNEPENVHYGIEHEISHIYNIAECKSKNCFQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g12195.t1 | CDD | cd04702 | ASRGL1_like | 23 | 306 | 7.1976E-133 |
| 6 | g12195.t1 | Gene3D | G3DSA:3.60.20.30 | (Glycosyl)asparaginase | 185 | 326 | 7.9E-44 |
| 2 | g12195.t1 | PANTHER | PTHR10188 | L-ASPARAGINASE | 22 | 317 | 5.4E-90 |
| 3 | g12195.t1 | PANTHER | PTHR10188:SF37 | SI:CH211-256M1.8 | 22 | 317 | 5.4E-90 |
| 1 | g12195.t1 | Pfam | PF01112 | Asparaginase | 22 | 311 | 4.5E-100 |
| 8 | g12195.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 9 | g12195.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 10 | g12195.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 13 | - |
| 11 | g12195.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 18 | - |
| 7 | g12195.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 336 | - |
| 4 | g12195.t1 | SUPERFAMILY | SSF56235 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) | 22 | 313 | 1.43E-83 |
| 5 | g12195.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016787 | hydrolase activity | MF |
| GO:0004067 | asparaginase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K13051 (L-asparaginase / beta-aspartyl-peptidase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed