Gene loci information

Transcript annotation

  • This transcript has been annotated as Probable isoaspartyl peptidase/L-asparaginase GA20639.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g12195 g12195.t3 TSS g12195.t3 21684910 21684910
chr_1 g12195 g12195.t3 isoform g12195.t3 21684937 21686260
chr_1 g12195 g12195.t3 exon g12195.t3.exon1 21684937 21685018
chr_1 g12195 g12195.t3 exon g12195.t3.exon2 21685144 21685662
chr_1 g12195 g12195.t3 cds g12195.t3.CDS1 21685266 21685662
chr_1 g12195 g12195.t3 exon g12195.t3.exon3 21685714 21685818
chr_1 g12195 g12195.t3 cds g12195.t3.CDS2 21685714 21685818
chr_1 g12195 g12195.t3 exon g12195.t3.exon4 21685965 21686260
chr_1 g12195 g12195.t3 cds g12195.t3.CDS3 21685965 21686260
chr_1 g12195 g12195.t3 TTS g12195.t3 21686317 21686317

Sequences

>g12195.t3 Gene=g12195 Length=1002
ATGAGGTTTTTGACATTAATTTCTCTTTTGACACTTTTTAGTTATAGCTTTGGCGGTAAT
GTGACAATTATTTGTCATGGAGCTAAATAAAATTTAAAGTTTATAGGAAAATACCGTGGC
ACTGTTCTTGCAGTTCAAATTGGTTATAACATTTTAAAAACTAATGGTACTTCTCTTGAT
GCTGTTGAGGCAGCTGTGCGAAGTATGGAAGTCGATGAAGAATTTAATGCTGGTTATGGA
AGTGTTTTGACAAGTGAAGGTAAAGTTGAAATGGATGCTTGCATCATGGACGGAGAAACA
ATGAAGGTTGGCGCAGTGACAGGAGTTCAAAATATTTTTCATACAATTACTCTTGCAAGA
AAAGTTATGGAAAAAACGAGATACAACTTTTTAAGCCCAGAAGGGGCAATGAAGCTTGCA
AAAGATGAAGGATTTCAATTTTTACCTGAAGGAACTTTAGTAACAGAGCGTGCAAAACTT
TCATTAGAAAATTGGAAACGAAATCAAAATCAAAGTTTAGGCGAGCAAGTTGGCGAGGGT
GGGACTGTAGGCTGTGCTGCCTACGATATCAATGGCAATTTAGCAGCAGCAACATCAACA
GGTGGCCTAACTGGAAAAATGAGTGGAAGAATCGGTGATACACCGATAGTTGGAGGAGGC
ACTTATGCGGACAATAAATTGGGAGCACTTTCTGCAACAGGCACTGGTGAAGTCATAATG
AAATCGGTACTTGTATATGACATTTTAAAACGATACGAATACAAGCAAATAAGTTTGCAA
CAAGCAGCACAAGAAGCATGTGATGATATGACTGAAAGATATGATGATGATGGGGGTGTT
ATTGGCCTCGACAAAGACGGTAATGTCGCAATTGGATTCTCTTCTCCTCAAATGTCTTGG
GCTTATCAAAATGAACCAGAAAATGTTCATTATGGAATTGAACATGAAATTTCTCACATT
TATAATATTGCAGAATGTAAGAGTAAAAATTGTTTCCAATAA

>g12195.t3 Gene=g12195 Length=265
MEVDEEFNAGYGSVLTSEGKVEMDACIMDGETMKVGAVTGVQNIFHTITLARKVMEKTRY
NFLSPEGAMKLAKDEGFQFLPEGTLVTERAKLSLENWKRNQNQSLGEQVGEGGTVGCAAY
DINGNLAAATSTGGLTGKMSGRIGDTPIVGGGTYADNKLGALSATGTGEVIMKSVLVYDI
LKRYEYKQISLQQAAQEACDDMTERYDDDGGVIGLDKDGNVAIGFSSPQMSWAYQNEPEN
VHYGIEHEISHIYNIAECKSKNCFQ

Protein features from InterProScan

Transcript Database ID Name Start End E.value
6 g12195.t3 CDD cd04702 ASRGL1_like 1 235 0
5 g12195.t3 Gene3D G3DSA:3.60.20.30 (Glycosyl)asparaginase 114 255 0
2 g12195.t3 PANTHER PTHR10188 L-ASPARAGINASE 1 246 0
3 g12195.t3 PANTHER PTHR10188:SF37 SI:CH211-256M1.8 1 246 0
1 g12195.t3 Pfam PF01112 Asparaginase 1 240 0
4 g12195.t3 SUPERFAMILY SSF56235 N-terminal nucleophile aminohydrolases (Ntn hydrolases) 1 243 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0016787 hydrolase activity MF
GO:0004067 asparaginase activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed