General information for g12197.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable isoaspartyl peptidase/L-asparaginase GA20639.
Parent gene
- The parent gene g12197 is located on chr_1: 21692323..21696078 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12197 | g12197.t1 | isoform | g12197.t1 | 21692323 | 21695033 |
| chr_1 | g12197 | g12197.t1 | exon | g12197.t1.exon1 | 21692323 | 21692359 |
| chr_1 | g12197 | g12197.t1 | cds | g12197.t1.CDS1 | 21692323 | 21692359 |
| chr_1 | g12197 | g12197.t1 | exon | g12197.t1.exon2 | 21692418 | 21692887 |
| chr_1 | g12197 | g12197.t1 | cds | g12197.t1.CDS2 | 21692418 | 21692887 |
| chr_1 | g12197 | g12197.t1 | exon | g12197.t1.exon3 | 21692954 | 21693026 |
| chr_1 | g12197 | g12197.t1 | cds | g12197.t1.CDS3 | 21692954 | 21693026 |
| chr_1 | g12197 | g12197.t1 | exon | g12197.t1.exon4 | 21694639 | 21695033 |
| chr_1 | g12197 | g12197.t1 | cds | g12197.t1.CDS4 | 21694639 | 21695033 |
| chr_1 | g12197 | g12197.t1 | TSS | g12197.t1 | NA | NA |
| chr_1 | g12197 | g12197.t1 | TTS | g12197.t1 | NA | NA |
Sequences
>g12197.t1 Gene=g12197 Length=975
ATGAAGGTTCAAATTTGCTTGGTTCATGGTGGTGCTGGAACAATTCCACAGAGTAGAGAG
GAGGGAAAGTTGAAAGGAGTGCAAAGAGCTGCATATATTGGTTATAAAACTTTAATAGAA
ACAGATAATGCAATTGAAGCAGTAGAGAAGGCAGTAAATGAGATGGAACTTGATCCTTAT
TTTAATGCAGGTTACGGAAGTGTACTCACAATAGATGGCAAAGTTGAAATGGATGCGAGT
ATAATGTGCGGAAGAACATTAAATTTTGGAGCAGTATCTTTAGTTCAAGATGTACTTCAC
CCAATTTCACTAGCTCGCTTAGTCATGCAAAAATCAAAACACAAATTTCTATCAGAAGAT
GGTGCCATAAAATTCGCACGACAAAATGGAATGAGAATCCTTCATCCACCTGGACAGCTA
GTCACTGATTTCACATCTAATGTAATACAGAATTATGTATCTGAACACAATGCACGCAAT
CAACGCAGTGAAATGGTTTTTGAAGCGCTTGGAGAAACTGGGACAGTGGGTGCTGTTGCG
ATCGATATTTTGGGAAACATTGCGGTAGCGACATCAACAGGTGGAATCGCGGGAAAAATG
AGTGGCAGAATAGGAGATACTCCATTGTTAAGTTCTGGAACATATTGTGATAATAAGTAT
GCAGGTATTTCTACAACAGGGCATGGTGATAGTTTAATGCGAGCTTGTTTAGCTCATGAT
GTTATAGCACGAATCAAATATTTGAATGAGGATATTGAAACTGCTGCTAAAAATGCTTGT
AAAAGAATGCATGATGATTTAAAAGCTACTGGAGGTATAATCGGCCTATCAAAAGATGGC
AAAGTAGGGATTGCTTTTACTAGTCAAAGAATGGCTTGGGCATATCAGAGAGGCTCAAAA
ATGCATTATGGTTTAAAACAAAACGACAATTTTGTAAAAGAAGTTGATTGCAATTTTTTA
AATAACAACCAATAA
>g12197.t1 Gene=g12197 Length=324
MKVQICLVHGGAGTIPQSREEGKLKGVQRAAYIGYKTLIETDNAIEAVEKAVNEMELDPY
FNAGYGSVLTIDGKVEMDASIMCGRTLNFGAVSLVQDVLHPISLARLVMQKSKHKFLSED
GAIKFARQNGMRILHPPGQLVTDFTSNVIQNYVSEHNARNQRSEMVFEALGETGTVGAVA
IDILGNIAVATSTGGIAGKMSGRIGDTPLLSSGTYCDNKYAGISTTGHGDSLMRACLAHD
VIARIKYLNEDIETAAKNACKRMHDDLKATGGIIGLSKDGKVGIAFTSQRMAWAYQRGSK
MHYGLKQNDNFVKEVDCNFLNNNQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g12197.t1 | CDD | cd04702 | ASRGL1_like | 7 | 299 | 3.66613E-132 |
| 6 | g12197.t1 | Coils | Coil | Coil | 38 | 58 | - |
| 5 | g12197.t1 | Gene3D | G3DSA:3.60.20.30 | (Glycosyl)asparaginase | 175 | 314 | 6.9E-42 |
| 2 | g12197.t1 | PANTHER | PTHR10188 | L-ASPARAGINASE | 5 | 315 | 1.8E-88 |
| 3 | g12197.t1 | PANTHER | PTHR10188:SF30 | ISOASPARTYL PEPTIDASE/L-ASPARAGINASE | 5 | 315 | 1.8E-88 |
| 1 | g12197.t1 | Pfam | PF01112 | Asparaginase | 7 | 303 | 9.2E-101 |
| 4 | g12197.t1 | SUPERFAMILY | SSF56235 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) | 5 | 304 | 8.23E-81 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016787 | hydrolase activity | MF |
| GO:0004067 | asparaginase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K13051 (L-asparaginase / beta-aspartyl-peptidase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
