General information for g12218.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Neutral alpha-glucosidase AB.
Parent gene
- The parent gene g12218 is located on chr_1: 21789945..21791376 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12218 | g12218.t1 | TSS | g12218.t1 | 21788052 | 21788052 |
| chr_1 | g12218 | g12218.t1 | isoform | g12218.t1 | 21788418 | 21791376 |
| chr_1 | g12218 | g12218.t1 | exon | g12218.t1.exon1 | 21788418 | 21788521 |
| chr_1 | g12218 | g12218.t1 | cds | g12218.t1.CDS1 | 21788418 | 21788521 |
| chr_1 | g12218 | g12218.t1 | exon | g12218.t1.exon2 | 21788583 | 21788724 |
| chr_1 | g12218 | g12218.t1 | cds | g12218.t1.CDS2 | 21788583 | 21788724 |
| chr_1 | g12218 | g12218.t1 | exon | g12218.t1.exon3 | 21788788 | 21791102 |
| chr_1 | g12218 | g12218.t1 | cds | g12218.t1.CDS3 | 21788788 | 21791102 |
| chr_1 | g12218 | g12218.t1 | exon | g12218.t1.exon4 | 21791163 | 21791376 |
| chr_1 | g12218 | g12218.t1 | cds | g12218.t1.CDS4 | 21791163 | 21791376 |
| chr_1 | g12218 | g12218.t1 | TTS | g12218.t1 | 21791521 | 21791521 |
Sequences
>g12218.t1 Gene=g12218 Length=2775
ATGAGTCGTAGACTGTGTCTCCTCATTTTCACTCTTTTCATTATAAATGCATTAGCTGTA
GACAAAAATAATTTTAAGACTTGTGAACAAGCCAGTTTTTGCAAACGTTCCCGTAATGTA
AATTATGAGAAATCACCTTACGAAATTGTGCCGAACACATTAAATATTAATGCAAACTAC
ATCACAGCTGATATAGTCAATAAAGAAAATGGACACTTGTTCGTGCTTAAACTTGAAGGC
ATCAAAGGAAATACATTCCATTTTGAAATTGATGAGAAAACTCCACTTAAGCCACGTTAT
CGTGTTGTAGATGCATTGCAAAAAGAACCAACATATGAGGCTGTTAAAGTATCGTCAAGC
ACATCTGACATAACTGTGACATGTGGCGAGAATAAAGCAGTTATATCGACTGCACCATTC
AAAATAGATTTCTATCAAAAAGATGTTTTGTCAGTAACTGCAAACAGTAAAGCAAAAATG
CATTTCGAACATTTACGTAAAAAATTAGAAGGCGAACAACATCAGCAAAATAATGAAAAT
GAAGAAAAAGACAATGTAGTTGATAATAATCAACAAATACAAGATGAACCAGGAACTTGG
GAAGAAAATTTTAAAGGACATCATGATACCAAACCTAACGGACCAACAGCAGTCGCTCTT
GATTTCACTTTTCATCAATCAAACGTTCTCTTTGGAATTCCTGAACATGCAGACTCATTT
GCTCTTAAAACAACAGTTGGAAATGGTGATCCTTATCGCTTATACAATTTAGATGTGTTT
GAGTATGAATTGAATAGCAGAATGGCATTATATGGTTCAGTTCCAGTTATTTATGGTCAT
GGTGAAAAAGGAACTACTGGAATTTTCTGGCAAAATGCTGCTGAAACATGGATTGATATT
GCAGGCACTTCAAATGAACAAAATGTTATGGAGTCAATAGTAAACTTTGTTGCTGGATCT
CGTAATCAAGATGCTCCTTCTGCTCATTTTATCTCAGAGTCTGGAATTATGGATGTTTTC
GTTCTTCTTGGACCAACACCAAATGAAGCTTTCAAACAATATACTGATCTCATTGGGCAT
GGAAATTTGCCACAAATGTTTGCAATTGGCTATCATCAATGCCGTTGGAATTATAATGAT
GAACAAGATGTCATGAGCGTTGCTCACAAATTTGACGAACACGATATTCCTATGGATACA
ATGTGGCTTGATATTGAATATACTGATAGCAAAAAATATTTCACTTGGGATCATCAAAAA
TTTCCACATCCAGTTGAAATGATTAAAAATCTCACTGACATGGGCAGACATTTGACTTTT
ATTATTGATCCTCATATTAAACGTGATTCCGGATATTTTTTCCACAATGATTGTACTGAT
CGTGGATATTATATTAAAACAAAAGAAGGAAAAGACTACGAGGGATGGTGTTGGCCTGGT
TCTGCAAGTTATCCTGATTTCTTTAATCCTGAAATTCGCAAATATTATGCCGATCAATAT
TTGATGGAAAATTTCCATGAAAATTCACCTGAAACAGGAATTTGGAATGATATGAATGAA
CCTTCAGTTTTTAATGGACCTGAAATTACAATGTTAAAGGATAATCTTCATTATGGTGGC
TGGGAACATCGTGATGTTCACAATCTTTATGGACAAATGCAACTTGTTGGAACTTTTGAT
GGATTGATTAGACGCAGTCAAAATCAATTGCGTCCATTTATTCTCACTCGTGCTCACTTT
TCTGGAACACAACGTTATGCTGCGGTATGGACAGGTGATAATGCAGCAGAATGGGGACAT
TTAGAAGCCGCAATTCCTATGTGTTTATCACTATCTGTTGCTGGAATTTCATTTTGCGGA
TCAGATGTTGGTGGTTTCTTTGGAAATCCTGAAGCTGAGCTTTTCTCTCGTTGGTATCAA
ACTAGTGCATTCATGCCATTCTTCAGAAGTCATGCTCATATTGATACTAAACGTCGTGAA
CCATGGTTATTCCCAGAAGAAACTAAGTTGATTATTCGTGATGCAATTAGAAAGCGTTAT
TCCTTCTTGCCTTTCTGGTATACAATTTTCTATGAACATGAACGCACTGGTATGCCTATT
ATGAGACCATTACTCGCTCAATATCCATTAGATAAGAATGTCTTTTCAATTGACACACAT
TTCATGTTGAGTGATAAACTATTAATTCGTCCTGTTATGCAAAAAGGAGCAAGCAAAGTT
GATGTTTATTTCCCATCACGCAATGGTGATAAACAAGGAGACATTTGGTATGATATTGAT
GATTATCGTAAAATCGATCATGTTGGAAGTGAAAGTGTAACAGTTAATGCTTATAAAGTT
CCCGTCTTTCAACGTGGTGGTACAATCATTCCAAGGAAGATGAGAATTCGTCGTTCAGCA
ATTCTTATGCATGATGATCCAATTACATTAGTTGTTGCTCTTGATGCTAAAAAACATGCC
AAAGGAACATTATATATTGATGATGAGAAGAGTTATGGCTATCGCCAAGGAAAATATTTG
TACTTAGAATTTGAATTTAAAGATGGTGTTTTGTCAAGCAGACTGATTGATGAAAGTGCA
TCATATAACACAAAAACATGGCTTGAACGAGTTATTATTGTCGGCTTAGATAAAGTGCCA
ACTGAAGCAACATTAAGAACATCATCGAATCAATCGACAAAACTTGAAATTGTTGATGCC
TCAAAGACTTCTTTCACAATTAGAAAGCCTGGTGTTAGCATGATGGAACCAACGTGGTCG
ATTACACTCAACTAA
>g12218.t1 Gene=g12218 Length=924
MSRRLCLLIFTLFIINALAVDKNNFKTCEQASFCKRSRNVNYEKSPYEIVPNTLNINANY
ITADIVNKENGHLFVLKLEGIKGNTFHFEIDEKTPLKPRYRVVDALQKEPTYEAVKVSSS
TSDITVTCGENKAVISTAPFKIDFYQKDVLSVTANSKAKMHFEHLRKKLEGEQHQQNNEN
EEKDNVVDNNQQIQDEPGTWEENFKGHHDTKPNGPTAVALDFTFHQSNVLFGIPEHADSF
ALKTTVGNGDPYRLYNLDVFEYELNSRMALYGSVPVIYGHGEKGTTGIFWQNAAETWIDI
AGTSNEQNVMESIVNFVAGSRNQDAPSAHFISESGIMDVFVLLGPTPNEAFKQYTDLIGH
GNLPQMFAIGYHQCRWNYNDEQDVMSVAHKFDEHDIPMDTMWLDIEYTDSKKYFTWDHQK
FPHPVEMIKNLTDMGRHLTFIIDPHIKRDSGYFFHNDCTDRGYYIKTKEGKDYEGWCWPG
SASYPDFFNPEIRKYYADQYLMENFHENSPETGIWNDMNEPSVFNGPEITMLKDNLHYGG
WEHRDVHNLYGQMQLVGTFDGLIRRSQNQLRPFILTRAHFSGTQRYAAVWTGDNAAEWGH
LEAAIPMCLSLSVAGISFCGSDVGGFFGNPEAELFSRWYQTSAFMPFFRSHAHIDTKRRE
PWLFPEETKLIIRDAIRKRYSFLPFWYTIFYEHERTGMPIMRPLLAQYPLDKNVFSIDTH
FMLSDKLLIRPVMQKGASKVDVYFPSRNGDKQGDIWYDIDDYRKIDHVGSESVTVNAYKV
PVFQRGGTIIPRKMRIRRSAILMHDDPITLVVALDAKKHAKGTLYIDDEKSYGYRQGKYL
YLEFEFKDGVLSSRLIDESASYNTKTWLERVIIVGLDKVPTEATLRTSSNQSTKLEIVDA
SKTSFTIRKPGVSMMEPTWSITLN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 19 | g12218.t1 | CDD | cd14752 | GH31_N | 216 | 359 | 3.39372E-24 |
| 20 | g12218.t1 | CDD | cd06603 | GH31_GANC_GANAB_alpha | 359 | 831 | 0.0 |
| 13 | g12218.t1 | Coils | Coil | Coil | 162 | 196 | - |
| 12 | g12218.t1 | Gene3D | G3DSA:3.20.20.80 | Glycosidases | 362 | 681 | 4.3E-110 |
| 10 | g12218.t1 | Gene3D | G3DSA:2.60.40.1180 | - | 683 | 786 | 1.2E-24 |
| 11 | g12218.t1 | Gene3D | G3DSA:2.60.40.1180 | - | 787 | 920 | 1.2E-15 |
| 23 | g12218.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 170 | 189 | - |
| 3 | g12218.t1 | PANTHER | PTHR22762 | ALPHA-GLUCOSIDASE | 12 | 912 | 0.0 |
| 4 | g12218.t1 | PANTHER | PTHR22762:SF54 | BCDNA.GH04962 | 12 | 912 | 0.0 |
| 1 | g12218.t1 | Pfam | PF13802 | Galactose mutarotase-like | 229 | 299 | 1.8E-22 |
| 2 | g12218.t1 | Pfam | PF01055 | Glycosyl hydrolases family 31 | 340 | 790 | 8.4E-143 |
| 15 | g12218.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 19 | - |
| 16 | g12218.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 17 | g12218.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 15 | - |
| 18 | g12218.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 19 | - |
| 14 | g12218.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 20 | 924 | - |
| 22 | g12218.t1 | ProSitePatterns | PS00129 | Glycosyl hydrolases family 31 active site. | 513 | 520 | - |
| 6 | g12218.t1 | SUPERFAMILY | SSF74650 | Galactose mutarotase-like | 54 | 347 | 1.54E-16 |
| 7 | g12218.t1 | SUPERFAMILY | SSF51445 | (Trans)glycosidases | 349 | 697 | 1.14E-82 |
| 5 | g12218.t1 | SUPERFAMILY | SSF51011 | Glycosyl hydrolase domain | 699 | 786 | 8.63E-13 |
| 9 | g12218.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 19 | - |
| 21 | g12218.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 19 | - |
| 8 | g12218.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 19 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0030246 | carbohydrate binding | MF |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds | MF |
| GO:0005975 | carbohydrate metabolic process | BP |
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
- This transcript has been annotated with K05546 (mannosyl-oligosaccharide alpha-1,3-glucosidase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
