General information for g12218.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Neutral alpha-glucosidase AB.
Parent gene
- The parent gene g12218 is located on chr_1: 21789945..21791376 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12218 | g12218.t5 | TSS | g12218.t5 | 21788052 | 21788052 |
| chr_1 | g12218 | g12218.t5 | isoform | g12218.t5 | 21788796 | 21789945 |
| chr_1 | g12218 | g12218.t5 | exon | g12218.t5.exon1 | 21788796 | 21789945 |
| chr_1 | g12218 | g12218.t5 | cds | g12218.t5.CDS1 | 21789019 | 21789945 |
| chr_1 | g12218 | g12218.t5 | TTS | g12218.t5 | NA | NA |
Sequences
>g12218.t5 Gene=g12218 Length=1150
ATTCCATTTTGAAATTGATGAGAAAACTCCACTTAAGCCACGTTATCGTGTTGTAGATGC
ATTGCAAAAAGAACCAACATATGAGGCTGTTAAAGTATCGTCAAGCACATCTGACATAAC
TGTGACATGTGGCGAGAATAAAGCAGTTATATCGACTGCACCATTCAAAATAGATTTCTA
TCAAAAAGATGTTTTGTCAGTAACTGCAAACAGTAAAGCAAAAATGCATTTCGAACATTT
ACGTAAAAAATTAGAAGGCGAACAACATCAGCAAAATAATGAAAATGAAGAAAAAGACAA
TGTAGTTGATAATAATCAACAAATACAAGATGAACCAGGAACTTGGGAAGAAAATTTTAA
AGGACATCATGATACCAAACCTAACGGACCAACAGCAGTCGCTCTTGATTTCACTTTTCA
TCAATCAAACGTTCTCTTTGGAATTCCTGAACATGCAGACTCATTTGCTCTTAAAACAAC
AGTTGGAAATGGTGATCCTTATCGCTTATACAATTTAGATGTGTTTGAGTATGAATTGAA
TAGCAGAATGGCATTATATGGTTCAGTTCCAGTTATTTATGGTCATGGTGAAAAAGGAAC
TACTGGAATTTTCTGGCAAAATGCTGCTGAAACATGGATTGATATTGCAGGCACTTCAAA
TGAACAAAATGTTATGGAGTCAATAGTAAACTTTGTTGCTGGATCTCGTAATCAAGATGC
TCCTTCTGCTCATTTTATCTCAGAGTCTGGAATTATGGATGTTTTCGTTCTTCTTGGACC
AACACCAAATGAAGCTTTCAAACAATATACTGATCTCATTGGGCATGGAAATTTGCCACA
AATGTTTGCAATTGGCTATCATCAATGCCGTTGGAATTATAATGATGAACAAGATGTCAT
GAGCGTTGCTCACAAATTTGACGAACACGATATTCCTATGGATACAATGTGGCTTGATAT
TGAATATACTGATAGCAAAAAATATTTCACTTGGGATCATCAAAAATTTCCACATCCAGT
TGAAATGATTAAAAATCTCACTGACATGGGCAGACATTTGACTTTTATTATTGATCCTCA
TATTAAACGTGATTCCGGATATTTTTTCCACAATGATTGTACTGATCGTGGATATTATAT
TAAAACAAAA
>g12218.t5 Gene=g12218 Length=309
MHFEHLRKKLEGEQHQQNNENEEKDNVVDNNQQIQDEPGTWEENFKGHHDTKPNGPTAVA
LDFTFHQSNVLFGIPEHADSFALKTTVGNGDPYRLYNLDVFEYELNSRMALYGSVPVIYG
HGEKGTTGIFWQNAAETWIDIAGTSNEQNVMESIVNFVAGSRNQDAPSAHFISESGIMDV
FVLLGPTPNEAFKQYTDLIGHGNLPQMFAIGYHQCRWNYNDEQDVMSVAHKFDEHDIPMD
TMWLDIEYTDSKKYFTWDHQKFPHPVEMIKNLTDMGRHLTFIIDPHIKRDSGYFFHNDCT
DRGYYIKTK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g12218.t5 | CDD | cd14752 | GH31_N | 57 | 200 | 9.95083E-25 |
| 10 | g12218.t5 | Coils | Coil | Coil | 3 | 37 | - |
| 8 | g12218.t5 | Gene3D | G3DSA:2.60.40.1760 | glycosyl hydrolase (family 31) | 4 | 201 | 1.4E-35 |
| 9 | g12218.t5 | Gene3D | G3DSA:3.20.20.80 | Glycosidases | 202 | 309 | 9.3E-37 |
| 7 | g12218.t5 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 30 | - |
| 3 | g12218.t5 | PANTHER | PTHR22762 | ALPHA-GLUCOSIDASE | 21 | 309 | 8.8E-108 |
| 4 | g12218.t5 | PANTHER | PTHR22762:SF54 | BCDNA.GH04962 | 21 | 309 | 8.8E-108 |
| 1 | g12218.t5 | Pfam | PF13802 | Galactose mutarotase-like | 70 | 140 | 3.5E-23 |
| 2 | g12218.t5 | Pfam | PF01055 | Glycosyl hydrolases family 31 | 181 | 308 | 7.7E-39 |
| 5 | g12218.t5 | SUPERFAMILY | SSF74650 | Galactose mutarotase-like | 55 | 188 | 1.04E-10 |
| 6 | g12218.t5 | SUPERFAMILY | SSF51445 | (Trans)glycosidases | 190 | 307 | 3.31E-18 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0030246 | carbohydrate binding | MF |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds | MF |
| GO:0005975 | carbohydrate metabolic process | BP |
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
