General information for g12218.t6
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Neutral alpha-glucosidase AB.
Parent gene
- The parent gene g12218 is located on chr_1: 21789945..21791376 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12218 | g12218.t6 | isoform | g12218.t6 | 21789945 | 21791376 |
| chr_1 | g12218 | g12218.t6 | exon | g12218.t6.exon1 | 21789945 | 21791102 |
| chr_1 | g12218 | g12218.t6 | cds | g12218.t6.CDS1 | 21790045 | 21791102 |
| chr_1 | g12218 | g12218.t6 | exon | g12218.t6.exon2 | 21791163 | 21791376 |
| chr_1 | g12218 | g12218.t6 | cds | g12218.t6.CDS2 | 21791163 | 21791376 |
| chr_1 | g12218 | g12218.t6 | TTS | g12218.t6 | 21791521 | 21791521 |
| chr_1 | g12218 | g12218.t6 | TSS | g12218.t6 | NA | NA |
Sequences
>g12218.t6 Gene=g12218 Length=1372
AGAAGGAAAAGACTACGAGGGATGGTGTTGGCCTGGTTCTGCAAGTTATCCTGATTTCTT
TAATCCTGAAATTCGCAAATATTATGCCGATCAATATTTGATGGAAAATTTCCATGAAAA
TTCACCTGAAACAGGAATTTGGAATGATATGAATGAACCTTCAGTTTTTAATGGACCTGA
AATTACAATGTTAAAGGATAATCTTCATTATGGTGGCTGGGAACATCGTGATGTTCACAA
TCTTTATGGACAAATGCAACTTGTTGGAACTTTTGATGGATTGATTAGACGCAGTCAAAA
TCAATTGCGTCCATTTATTCTCACTCGTGCTCACTTTTCTGGAACACAACGTTATGCTGC
GGTATGGACAGGTGATAATGCAGCAGAATGGGGACATTTAGAAGCCGCAATTCCTATGTG
TTTATCACTATCTGTTGCTGGAATTTCATTTTGCGGATCAGATGTTGGTGGTTTCTTTGG
AAATCCTGAAGCTGAGCTTTTCTCTCGTTGGTATCAAACTAGTGCATTCATGCCATTCTT
CAGAAGTCATGCTCATATTGATACTAAACGTCGTGAACCATGGTTATTCCCAGAAGAAAC
TAAGTTGATTATTCGTGATGCAATTAGAAAGCGTTATTCCTTCTTGCCTTTCTGGTATAC
AATTTTCTATGAACATGAACGCACTGGTATGCCTATTATGAGACCATTACTCGCTCAATA
TCCATTAGATAAGAATGTCTTTTCAATTGACACACATTTCATGTTGAGTGATAAACTATT
AATTCGTCCTGTTATGCAAAAAGGAGCAAGCAAAGTTGATGTTTATTTCCCATCACGCAA
TGGTGATAAACAAGGAGACATTTGGTATGATATTGATGATTATCGTAAAATCGATCATGT
TGGAAGTGAAAGTGTAACAGTTAATGCTTATAAAGTTCCCGTCTTTCAACGTGGTGGTAC
AATCATTCCAAGGAAGATGAGAATTCGTCGTTCAGCAATTCTTATGCATGATGATCCAAT
TACATTAGTTGTTGCTCTTGATGCTAAAAAACATGCCAAAGGAACATTATATATTGATGA
TGAGAAGAGTTATGGCTATCGCCAAGGAAAATATTTGTACTTAGAATTTGAATTTAAAGA
TGGTGTTTTGTCAAGCAGACTGATTGATGAAAGTGCATCATATAACACAAAAACATGGCT
TGAACGAGTTATTATTGTCGGCTTAGATAAAGTGCCAACTGAAGCAACATTAAGAACATC
ATCGAATCAATCGACAAAACTTGAAATTGTTGATGCCTCAAAGACTTCTTTCACAATTAG
AAAGCCTGGTGTTAGCATGATGGAACCAACGTGGTCGATTACACTCAACTAA
>g12218.t6 Gene=g12218 Length=423
MENFHENSPETGIWNDMNEPSVFNGPEITMLKDNLHYGGWEHRDVHNLYGQMQLVGTFDG
LIRRSQNQLRPFILTRAHFSGTQRYAAVWTGDNAAEWGHLEAAIPMCLSLSVAGISFCGS
DVGGFFGNPEAELFSRWYQTSAFMPFFRSHAHIDTKRREPWLFPEETKLIIRDAIRKRYS
FLPFWYTIFYEHERTGMPIMRPLLAQYPLDKNVFSIDTHFMLSDKLLIRPVMQKGASKVD
VYFPSRNGDKQGDIWYDIDDYRKIDHVGSESVTVNAYKVPVFQRGGTIIPRKMRIRRSAI
LMHDDPITLVVALDAKKHAKGTLYIDDEKSYGYRQGKYLYLEFEFKDGVLSSRLIDESAS
YNTKTWLERVIIVGLDKVPTEATLRTSSNQSTKLEIVDASKTSFTIRKPGVSMMEPTWSI
TLN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g12218.t6 | CDD | cd06603 | GH31_GANC_GANAB_alpha | 2 | 330 | 0.0 |
| 9 | g12218.t6 | Gene3D | G3DSA:3.20.20.80 | Glycosidases | 1 | 201 | 1.8E-70 |
| 7 | g12218.t6 | Gene3D | G3DSA:2.60.40.1180 | - | 202 | 284 | 9.3E-18 |
| 8 | g12218.t6 | Gene3D | G3DSA:2.60.40.1180 | - | 286 | 419 | 3.3E-16 |
| 2 | g12218.t6 | PANTHER | PTHR22762 | ALPHA-GLUCOSIDASE | 2 | 411 | 5.8E-168 |
| 3 | g12218.t6 | PANTHER | PTHR22762:SF54 | BCDNA.GH04962 | 2 | 411 | 5.8E-168 |
| 1 | g12218.t6 | Pfam | PF01055 | Glycosyl hydrolases family 31 | 7 | 289 | 2.0E-90 |
| 6 | g12218.t6 | ProSitePatterns | PS00129 | Glycosyl hydrolases family 31 active site. | 12 | 19 | - |
| 5 | g12218.t6 | SUPERFAMILY | SSF51445 | (Trans)glycosidases | 11 | 196 | 6.74E-50 |
| 4 | g12218.t6 | SUPERFAMILY | SSF51011 | Glycosyl hydrolase domain | 198 | 285 | 2.59E-13 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds | MF |
| GO:0005975 | carbohydrate metabolic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
