General information for g12258.t14
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Ero1-like protein.
Parent gene
- The parent gene g12258 is located on chr_1: 22046003..22047141 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12258 | g12258.t14 | TTS | g12258.t14 | 22045616 | 22045616 |
| chr_1 | g12258 | g12258.t14 | isoform | g12258.t14 | 22046003 | 22046827 |
| chr_1 | g12258 | g12258.t14 | exon | g12258.t14.exon1 | 22046003 | 22046415 |
| chr_1 | g12258 | g12258.t14 | cds | g12258.t14.CDS1 | 22046005 | 22046415 |
| chr_1 | g12258 | g12258.t14 | exon | g12258.t14.exon2 | 22046711 | 22046827 |
| chr_1 | g12258 | g12258.t14 | cds | g12258.t14.CDS2 | 22046711 | 22046827 |
| chr_1 | g12258 | g12258.t14 | TSS | g12258.t14 | 22047008 | 22047008 |
Sequences
>g12258.t14 Gene=g12258 Length=530
ATGTCGAGAAAATGTTTCATAATTTTCCTTCTACTATTTAATGTTTATCAGATATCACAA
CAATTTTTCCAATCTCCAGAGGATAGCAGCACGCCAGATAAGCAATGTTTTTGTGAGCTA
AAAGGCAACGTAGATGACTGCAGTTGTACAGTAGATACTGTCGATTACTACAACAATGTT
AAAATATATCCACGCTTGAAAAGCTTGCTTCAGAGCAATTACTTTCGATTCTACAAAGTA
AATCTTCACAATGAATGTCCATTTTGGAAGGATGTTGATTTTGAATGTGCTATGAAATTT
TGTCATGTGAAGTCATGTGAAGACAAAGATTTACCACTTGGATTCAAAGGAGCAGCAGAA
AGTCCTCACTCGAAATACATAAAAGAAGCTCAAACAATTGGCTGTCAAGAAGATTACAAT
AAAGAACTTGACTTTCTTAATAAATCAATCAGCGAGAAACAACATCAGGAGCTTAAAAAA
TGGTCAGAGCATGATGAAGCAAAGGACAGTTTTTGTGTTTTAGATGACGA
>g12258.t14 Gene=g12258 Length=176
MSRKCFIIFLLLFNVYQISQQFFQSPEDSSTPDKQCFCELKGNVDDCSCTVDTVDYYNNV
KIYPRLKSLLQSNYFRFYKVNLHNECPFWKDVDFECAMKFCHVKSCEDKDLPLGFKGAAE
SPHSKYIKEAQTIGCQEDYNKELDFLNKSISEKQHQELKKWSEHDEAKDSFCVLDD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g12258.t14 | Coils | Coil | Coil | 136 | 156 | - |
| 2 | g12258.t14 | PANTHER | PTHR12613:SF1 | ERO1-LIKE PROTEIN ALPHA | 14 | 176 | 5.5E-40 |
| 3 | g12258.t14 | PANTHER | PTHR12613 | ERO1-RELATED | 14 | 176 | 5.5E-40 |
| 1 | g12258.t14 | Pfam | PF04137 | Endoplasmic Reticulum Oxidoreductin 1 (ERO1) | 61 | 176 | 9.5E-20 |
| 8 | g12258.t14 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 17 | - |
| 9 | g12258.t14 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 10 | g12258.t14 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 13 | - |
| 11 | g12258.t14 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 17 | - |
| 7 | g12258.t14 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 18 | 176 | - |
| 4 | g12258.t14 | SUPERFAMILY | SSF110019 | ERO1-like | 49 | 176 | 6.8E-22 |
| 5 | g12258.t14 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 20 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005783 | endoplasmic reticulum | CC |
| GO:0016671 | oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor | MF |
| GO:0003756 | protein disulfide isomerase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
