General information for g12301.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Kinesin heavy chain.
Parent gene
- The parent gene g12301 is located on chr_1: 22272371..22273098 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12301 | g12301.t1 | TSS | g12301.t1 | 22266720 | 22266720 |
| chr_1 | g12301 | g12301.t1 | isoform | g12301.t1 | 22266866 | 22273098 |
| chr_1 | g12301 | g12301.t1 | exon | g12301.t1.exon1 | 22266866 | 22267015 |
| chr_1 | g12301 | g12301.t1 | cds | g12301.t1.CDS1 | 22266866 | 22267015 |
| chr_1 | g12301 | g12301.t1 | exon | g12301.t1.exon2 | 22268130 | 22268589 |
| chr_1 | g12301 | g12301.t1 | cds | g12301.t1.CDS2 | 22268130 | 22268589 |
| chr_1 | g12301 | g12301.t1 | exon | g12301.t1.exon3 | 22268656 | 22270291 |
| chr_1 | g12301 | g12301.t1 | cds | g12301.t1.CDS3 | 22268656 | 22270291 |
| chr_1 | g12301 | g12301.t1 | exon | g12301.t1.exon4 | 22270390 | 22270491 |
| chr_1 | g12301 | g12301.t1 | cds | g12301.t1.CDS4 | 22270390 | 22270491 |
| chr_1 | g12301 | g12301.t1 | exon | g12301.t1.exon5 | 22272371 | 22272828 |
| chr_1 | g12301 | g12301.t1 | cds | g12301.t1.CDS5 | 22272371 | 22272828 |
| chr_1 | g12301 | g12301.t1 | exon | g12301.t1.exon6 | 22272891 | 22273016 |
| chr_1 | g12301 | g12301.t1 | cds | g12301.t1.CDS6 | 22272891 | 22273016 |
| chr_1 | g12301 | g12301.t1 | exon | g12301.t1.exon7 | 22273079 | 22273098 |
| chr_1 | g12301 | g12301.t1 | cds | g12301.t1.CDS7 | 22273079 | 22273098 |
| chr_1 | g12301 | g12301.t1 | TTS | g12301.t1 | 22273220 | 22273220 |
Sequences
>g12301.t1 Gene=g12301 Length=2952
ATGGCGGCAGTAGACCGAGAAATCCCAGCTGAGGATTCTATTAAAGTCGTCTGTCGCTTT
AGGCCACTCAATGATAGTGAGGAGAAAGCTGGATCTAAATTTATTGTCAAATTCCCGCAA
GGTCCCGAGGAAAACTGTATATCAATTGGGGGCAAAGTTTATTTGTTTGATAAGGTCTTC
AAACCTAATGCAACTCAAGAAAAAGTATATAGTGAAGCCGCAAAATCTATTGTCGCTGAT
GTGCTTGCCGGCTACAATGGAACAATTTTCGCTTATGGTCAAACATCTAGTGGTAAAACA
CACACAATGGAAGGAGTTATTGGTGATTCAGTTAAGCAGGGCATCATTCCACGAATTGTC
AACGATATTTTTAATCACATTTATACGATGGAAATGAATTTAGAATTTCACATCAAAGTA
TCCTATTATGAAATCTATATGGATAAAATTCGTGATTTATTGGATGTGTCAAAAGTAAAT
TTGAGTGTGCACGAAGATAAAAATCGTGTTCCATATGTGAAAGGTGCTACTGAAAGATTT
GTATCGAGTCCCGAAGATGTCTTTGAAGTTATTGAAGAAGGAAAATCTAATCGTCATATT
GCTGTTACAAATATGAATGAACATTCATCACGTTCACATTCGGTTTTCTTAATTAATGTA
AAACAGGAAAATCTTGAAAATCAAAAGAAATTATCTGGAAAATTGTACTTGGTTGATTTG
GCTGGTAGTGAAAAAGTATCAAAAACTGGCGCGGAAGGAACAGTGTTAGATGAAGCAAAG
AACATTAACAAGTCACTTTCAGCACTTGGTAATGTCATTTCAGCGTTGGCTGATGGCAAT
AAAAGTCACATTCCATATCGTGACTCAAAATTGACGAGAATTTTGCAAGAATCACTGGGA
GGAAATGCTCGAACAACAATTGTCATTTGTTGCTCGCCAGCAAGTTTCAATGAGTCGGAA
ACAAAATCAACATTGGATTTTGGTAAGCGTGCTAAAACTGTCAAGAATGTTGTATGTGTC
AATGAGGAACTTACTGCCGAGGAATGGAAACGTCGTTATGAACGTGAAAAGGAAAAAAAT
GCAAGACTTAAAGGAAAACTTGAGAAACTTGAAGCTGAATTGGCAAGATGGAGAGCAGGT
GAAAGTGTTGATGTTACAGAGCAATTTAATATGCAAGATCCAATGGAAGCAAGTACACCA
AATGTTGAAGCAGAAACTGCCCCTCGTGAGGGACCAGTGCCTGCAACTCCTAGTGGACTC
CTTATTGGCTCGATTTCAACTGATGAGCGAGCTCGTCTTGAGAGTGAACGTGAGCGATTG
TATCTTCAATTGGATGAGAAAGATGAAGAAATTAATCAACAATCACAATATGTTGAGAAG
CTCAAGGAGCAAATTATGGATCAAGAAGAACTTATTGCCAATTCACGTCGTGATTATGAA
AATTTGCAGACAGAAATGACACGCATTCAACAAGAGAACGAGAGTGCAAAGGAAGAAGTA
AAAGAAGTGCTTCAAGCATTAGAAGAATTGGCTGTTAATTACGATCAAAAATCACAAGAA
ATTGATAACAAAAATAAAGAACTTGAATCAATTAATGAAGAACTTCAACAGAAACAGTCA
TTGTTAAATACAACATCAACAGAACTTCAACAATTAAAGGATATGTCAAATCATCAAAAG
AAACGAATTATTGACCTTTTGGGTAATCTATTGCGTGAACTTTGTGAAGTCGGTCAAGCA
CTCAATACAGAAACGTCAGCTGAATTGAAAATTAATGCCGATGCAAATATCAATAAAATT
GAAGAAGAGTTTACAGTTGCACGCTTATATATTAGTAAAATGAAGTCCGAAGCAAAGAAC
ATCACACAGCGCTGTAGTAATTTGGAAACACAACAGCAAACGACATCGAAAAAAATTACC
GAGTACGAGAAAGATTTGAGCGATTGTCGGTTATTGATCTCTCAGCATGAGGCTCGCATG
AAATCTCTTCAAGAGTCGATGCGTGAATCCGAGAACAAAAAGCGATCATTGGAAGAGAAT
GTTGATGCATTGCGTGAAGAATGTGCGAAATTAAAAGCGGCTGAAAAATTGACAACTGTG
AATGTAGAAGAGAAGCAGAAAGCAGAACAATTGAGAGTTGAATTTGTTGAGCAGATGGAT
CAATTAAGAGATGTTCACACGAAACAAGTTGCAGATTTGAGAGATGAAATTAGTGAAAAA
CAAGAGCAAATTAATGAGTTGAAGGACGTCAATCAAAAGTTGACATTAGCACATCAACAA
ATGTCCAACGATCACGAAAAGTTGAAACAAGAGGAGGCCGAAAAATCAGCTCGTTTGCAG
GAACTCATATTGACGAACGAGCGTCGAGAACAGGCAAGAAAGGACTTGAAAGGATTGGAG
GACACAGTTTCAAAAGAGCTTCAAACCTTGCACAATTTGAGAAAATTGTTTGTTCAAGAT
TTACAAGCTCGTATCAAGAAATCTATCAACTCAGAAGATAGCGAGGACGATGGAGGTTCC
TTAGCTCAAAAACAAAAGATTTCCTTCTTGGAAAATAATTTGGAGCAATTAACGAAAGTT
CATAAGCAACTCGTTCGTGACAATGCTGATTTGAGGTGTGAATTGCCTAAACTTGAGAAG
AGATTACGAACGACAATGGAACGGGTAAAAGCTCTCGAGACAGCATTGAAAGAGGCTAAA
GAGGGAGCAATGCGCGATCGCAAACGATACCAATATGAAGTTGACCGAATCAAAGAAGCC
GTACGTCAAAAGAATTTGGCAAGACGTGGACCTCAAGCACAAATTGCAAAACCAATTCGT
GCTGGTCACGCTCCTCCAGTTTCAGGAATTAGAGGAGGAGGCATTGTTCCTCAACAAGGT
CAGCCACAGAAAATCATTTCAGAAGACAGTCTTAGAAAGCAATCAATGAAGGATTTAATG
GAAAAAAGTTGA
>g12301.t1 Gene=g12301 Length=983
MAAVDREIPAEDSIKVVCRFRPLNDSEEKAGSKFIVKFPQGPEENCISIGGKVYLFDKVF
KPNATQEKVYSEAAKSIVADVLAGYNGTIFAYGQTSSGKTHTMEGVIGDSVKQGIIPRIV
NDIFNHIYTMEMNLEFHIKVSYYEIYMDKIRDLLDVSKVNLSVHEDKNRVPYVKGATERF
VSSPEDVFEVIEEGKSNRHIAVTNMNEHSSRSHSVFLINVKQENLENQKKLSGKLYLVDL
AGSEKVSKTGAEGTVLDEAKNINKSLSALGNVISALADGNKSHIPYRDSKLTRILQESLG
GNARTTIVICCSPASFNESETKSTLDFGKRAKTVKNVVCVNEELTAEEWKRRYEREKEKN
ARLKGKLEKLEAELARWRAGESVDVTEQFNMQDPMEASTPNVEAETAPREGPVPATPSGL
LIGSISTDERARLESERERLYLQLDEKDEEINQQSQYVEKLKEQIMDQEELIANSRRDYE
NLQTEMTRIQQENESAKEEVKEVLQALEELAVNYDQKSQEIDNKNKELESINEELQQKQS
LLNTTSTELQQLKDMSNHQKKRIIDLLGNLLRELCEVGQALNTETSAELKINADANINKI
EEEFTVARLYISKMKSEAKNITQRCSNLETQQQTTSKKITEYEKDLSDCRLLISQHEARM
KSLQESMRESENKKRSLEENVDALREECAKLKAAEKLTTVNVEEKQKAEQLRVEFVEQMD
QLRDVHTKQVADLRDEISEKQEQINELKDVNQKLTLAHQQMSNDHEKLKQEEAEKSARLQ
ELILTNERREQARKDLKGLEDTVSKELQTLHNLRKLFVQDLQARIKKSINSEDSEDDGGS
LAQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRTTMERVKALETALKEAK
EGAMRDRKRYQYEVDRIKEAVRQKNLARRGPQAQIAKPIRAGHAPPVSGIRGGGIVPQQG
QPQKIISEDSLRKQSMKDLMEKS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g12301.t1 | CDD | cd01369 | KISc_KHC_KIF5 | 11 | 334 | 0.0 |
| 13 | g12301.t1 | Coils | Coil | Coil | 339 | 380 | - |
| 10 | g12301.t1 | Coils | Coil | Coil | 430 | 555 | - |
| 14 | g12301.t1 | Coils | Coil | Coil | 611 | 631 | - |
| 12 | g12301.t1 | Coils | Coil | Coil | 653 | 809 | - |
| 11 | g12301.t1 | Coils | Coil | Coil | 847 | 902 | - |
| 9 | g12301.t1 | Gene3D | G3DSA:3.40.850.10 | Kinesin | 3 | 366 | 6.7E-161 |
| 19 | g12301.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 392 | 423 | - |
| 18 | g12301.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 942 | 983 | - |
| 2 | g12301.t1 | PANTHER | PTHR47968 | CENTROMERE PROTEIN E | 8 | 947 | 0.0 |
| 3 | g12301.t1 | PANTHER | PTHR47968:SF31 | KINESIN FAMILY MEMBER 5A | 8 | 947 | 0.0 |
| 7 | g12301.t1 | PRINTS | PR00380 | Kinesin heavy chain signature | 84 | 105 | 1.8E-43 |
| 6 | g12301.t1 | PRINTS | PR00380 | Kinesin heavy chain signature | 203 | 220 | 1.8E-43 |
| 5 | g12301.t1 | PRINTS | PR00380 | Kinesin heavy chain signature | 234 | 252 | 1.8E-43 |
| 4 | g12301.t1 | PRINTS | PR00380 | Kinesin heavy chain signature | 284 | 305 | 1.8E-43 |
| 1 | g12301.t1 | Pfam | PF00225 | Kinesin motor domain | 19 | 334 | 8.9E-112 |
| 17 | g12301.t1 | ProSitePatterns | PS00411 | Kinesin motor domain signature. | 233 | 244 | - |
| 20 | g12301.t1 | ProSiteProfiles | PS50067 | Kinesin motor domain profile. | 13 | 334 | 125.333 |
| 16 | g12301.t1 | SMART | SM00129 | kinesin_4 | 11 | 342 | 2.0E-177 |
| 8 | g12301.t1 | SUPERFAMILY | SSF52540 | P-loop containing nucleoside triphosphate hydrolases | 10 | 353 | 1.96E-122 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:1990939 | NA | NA |
| GO:0007018 | microtubule-based movement | BP |
| GO:0005524 | ATP binding | MF |
| GO:0003777 | microtubule motor activity | MF |
| GO:0008017 | microtubule binding | MF |
KEGG
Orthology
- This transcript has been annotated with K10396 (kinesin family member 5)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
