General information for g12301.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Kinesin heavy chain.
Parent gene
- The parent gene g12301 is located on chr_1: 22272371..22273098 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12301 | g12301.t2 | TSS | g12301.t2 | 22266720 | 22266720 |
| chr_1 | g12301 | g12301.t2 | isoform | g12301.t2 | 22266866 | 22269720 |
| chr_1 | g12301 | g12301.t2 | exon | g12301.t2.exon1 | 22266866 | 22267015 |
| chr_1 | g12301 | g12301.t2 | cds | g12301.t2.CDS1 | 22266866 | 22267015 |
| chr_1 | g12301 | g12301.t2 | exon | g12301.t2.exon2 | 22268130 | 22268589 |
| chr_1 | g12301 | g12301.t2 | cds | g12301.t2.CDS2 | 22268130 | 22268589 |
| chr_1 | g12301 | g12301.t2 | exon | g12301.t2.exon3 | 22268656 | 22269720 |
| chr_1 | g12301 | g12301.t2 | cds | g12301.t2.CDS3 | 22268656 | 22269719 |
| chr_1 | g12301 | g12301.t2 | TTS | g12301.t2 | NA | NA |
Sequences
>g12301.t2 Gene=g12301 Length=1675
ATGGCGGCAGTAGACCGAGAAATCCCAGCTGAGGATTCTATTAAAGTCGTCTGTCGCTTT
AGGCCACTCAATGATAGTGAGGAGAAAGCTGGATCTAAATTTATTGTCAAATTCCCGCAA
GGTCCCGAGGAAAACTGTATATCAATTGGGGGCAAAGTTTATTTGTTTGATAAGGTCTTC
AAACCTAATGCAACTCAAGAAAAAGTATATAGTGAAGCCGCAAAATCTATTGTCGCTGAT
GTGCTTGCCGGCTACAATGGAACAATTTTCGCTTATGGTCAAACATCTAGTGGTAAAACA
CACACAATGGAAGGAGTTATTGGTGATTCAGTTAAGCAGGGCATCATTCCACGAATTGTC
AACGATATTTTTAATCACATTTATACGATGGAAATGAATTTAGAATTTCACATCAAAGTA
TCCTATTATGAAATCTATATGGATAAAATTCGTGATTTATTGGATGTGTCAAAAGTAAAT
TTGAGTGTGCACGAAGATAAAAATCGTGTTCCATATGTGAAAGGTGCTACTGAAAGATTT
GTATCGAGTCCCGAAGATGTCTTTGAAGTTATTGAAGAAGGAAAATCTAATCGTCATATT
GCTGTTACAAATATGAATGAACATTCATCACGTTCACATTCGGTTTTCTTAATTAATGTA
AAACAGGAAAATCTTGAAAATCAAAAGAAATTATCTGGAAAATTGTACTTGGTTGATTTG
GCTGGTAGTGAAAAAGTATCAAAAACTGGCGCGGAAGGAACAGTGTTAGATGAAGCAAAG
AACATTAACAAGTCACTTTCAGCACTTGGTAATGTCATTTCAGCGTTGGCTGATGGCAAT
AAAAGTCACATTCCATATCGTGACTCAAAATTGACGAGAATTTTGCAAGAATCACTGGGA
GGAAATGCTCGAACAACAATTGTCATTTGTTGCTCGCCAGCAAGTTTCAATGAGTCGGAA
ACAAAATCAACATTGGATTTTGGTAAGCGTGCTAAAACTGTCAAGAATGTTGTATGTGTC
AATGAGGAACTTACTGCCGAGGAATGGAAACGTCGTTATGAACGTGAAAAGGAAAAAAAT
GCAAGACTTAAAGGAAAACTTGAGAAACTTGAAGCTGAATTGGCAAGATGGAGAGCAGGT
GAAAGTGTTGATGTTACAGAGCAATTTAATATGCAAGATCCAATGGAAGCAAGTACACCA
AATGTTGAAGCAGAAACTGCCCCTCGTGAGGGACCAGTGCCTGCAACTCCTAGTGGACTC
CTTATTGGCTCGATTTCAACTGATGAGCGAGCTCGTCTTGAGAGTGAACGTGAGCGATTG
TATCTTCAATTGGATGAGAAAGATGAAGAAATTAATCAACAATCACAATATGTTGAGAAG
CTCAAGGAGCAAATTATGGATCAAGAAGAACTTATTGCCAATTCACGTCGTGATTATGAA
AATTTGCAGACAGAAATGACACGCATTCAACAAGAGAACGAGAGTGCAAAGGAAGAAGTA
AAAGAAGTGCTTCAAGCATTAGAAGAATTGGCTGTTAATTACGATCAAAAATCACAAGAA
ATTGATAACAAAAATAAAGAACTTGAATCAATTAATGAAGAACTTCAACAGAAACAGTCA
TTGTTAAATACAACATCAACAGAACTTCAACAATTAAAGGATATGTCAAATCATC
>g12301.t2 Gene=g12301 Length=558
MAAVDREIPAEDSIKVVCRFRPLNDSEEKAGSKFIVKFPQGPEENCISIGGKVYLFDKVF
KPNATQEKVYSEAAKSIVADVLAGYNGTIFAYGQTSSGKTHTMEGVIGDSVKQGIIPRIV
NDIFNHIYTMEMNLEFHIKVSYYEIYMDKIRDLLDVSKVNLSVHEDKNRVPYVKGATERF
VSSPEDVFEVIEEGKSNRHIAVTNMNEHSSRSHSVFLINVKQENLENQKKLSGKLYLVDL
AGSEKVSKTGAEGTVLDEAKNINKSLSALGNVISALADGNKSHIPYRDSKLTRILQESLG
GNARTTIVICCSPASFNESETKSTLDFGKRAKTVKNVVCVNEELTAEEWKRRYEREKEKN
ARLKGKLEKLEAELARWRAGESVDVTEQFNMQDPMEASTPNVEAETAPREGPVPATPSGL
LIGSISTDERARLESERERLYLQLDEKDEEINQQSQYVEKLKEQIMDQEELIANSRRDYE
NLQTEMTRIQQENESAKEEVKEVLQALEELAVNYDQKSQEIDNKNKELESINEELQQKQS
LLNTTSTELQQLKDMSNH
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g12301.t2 | CDD | cd01369 | KISc_KHC_KIF5 | 11 | 334 | 0.0 |
| 11 | g12301.t2 | Coils | Coil | Coil | 339 | 380 | - |
| 10 | g12301.t2 | Coils | Coil | Coil | 430 | 555 | - |
| 9 | g12301.t2 | Gene3D | G3DSA:3.40.850.10 | Kinesin | 3 | 366 | 1.6E-161 |
| 15 | g12301.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 392 | 423 | - |
| 2 | g12301.t2 | PANTHER | PTHR24115 | KINESIN-RELATED | 8 | 558 | 4.0E-252 |
| 3 | g12301.t2 | PANTHER | PTHR24115:SF927 | KINESIN FAMILY MEMBER 5A, A-RELATED | 8 | 558 | 4.0E-252 |
| 7 | g12301.t2 | PRINTS | PR00380 | Kinesin heavy chain signature | 84 | 105 | 2.2E-44 |
| 6 | g12301.t2 | PRINTS | PR00380 | Kinesin heavy chain signature | 203 | 220 | 2.2E-44 |
| 5 | g12301.t2 | PRINTS | PR00380 | Kinesin heavy chain signature | 234 | 252 | 2.2E-44 |
| 4 | g12301.t2 | PRINTS | PR00380 | Kinesin heavy chain signature | 284 | 305 | 2.2E-44 |
| 1 | g12301.t2 | Pfam | PF00225 | Kinesin motor domain | 19 | 334 | 2.4E-112 |
| 14 | g12301.t2 | ProSitePatterns | PS00411 | Kinesin motor domain signature. | 233 | 244 | - |
| 16 | g12301.t2 | ProSiteProfiles | PS50067 | Kinesin motor domain profile. | 13 | 334 | 125.333 |
| 13 | g12301.t2 | SMART | SM00129 | kinesin_4 | 11 | 342 | 2.0E-177 |
| 8 | g12301.t2 | SUPERFAMILY | SSF52540 | P-loop containing nucleoside triphosphate hydrolases | 10 | 353 | 4.69E-123 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:1990939 | NA | NA |
| GO:0007018 | microtubule-based movement | BP |
| GO:0005524 | ATP binding | MF |
| GO:0003777 | microtubule motor activity | MF |
| GO:0008017 | microtubule binding | MF |
KEGG
Orthology
- This transcript has been annotated with K10396 (kinesin family member 5)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed