General information for g12301.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Kinesin heavy chain.
Parent gene
- The parent gene g12301 is located on chr_1: 22272371..22273098 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12301 | g12301.t3 | TSS | g12301.t3 | 22266720 | 22266720 |
| chr_1 | g12301 | g12301.t3 | isoform | g12301.t3 | 22267016 | 22269109 |
| chr_1 | g12301 | g12301.t3 | exon | g12301.t3.exon1 | 22267016 | 22267522 |
| chr_1 | g12301 | g12301.t3 | exon | g12301.t3.exon2 | 22268130 | 22268589 |
| chr_1 | g12301 | g12301.t3 | cds | g12301.t3.CDS1 | 22268286 | 22268589 |
| chr_1 | g12301 | g12301.t3 | exon | g12301.t3.exon3 | 22268656 | 22269109 |
| chr_1 | g12301 | g12301.t3 | cds | g12301.t3.CDS2 | 22268656 | 22269107 |
| chr_1 | g12301 | g12301.t3 | TTS | g12301.t3 | 22269110 | 22269110 |
Sequences
>g12301.t3 Gene=g12301 Length=1421
GTAAGTTTCAGTCATTAACAAAAGTATTTGCTCGATTTTTCATAAAACACGACTCATAAA
TGGTGATGAGGAGGAAATTTTTTTTCCATTGCCACGCCAGTTTTACAAAAATCGTCTATA
GCGAATAATCCATGTGAAGTAAATTTTGTGCGCTTTGAGCTTCATAAAACAAAAACTGTT
ATTTCATTGTAATGACTTAATCTATTTTATAACTGCACCATAAAATTTCATTCATTATCA
CTATTCTCGTGTGATGCTCAATTAATAAAACAAACAATTTTTTCTTTGAAATACAAGAAT
GCATAAGTGATTTAGGCAAAATACTTTTTATATTGTATCCATCTTGAATTTTCTTTTTCT
AAAAATTATTTTGGTCTATTGTAAAGAAGAAAAATTTACATTCCATGCTAAAGACAATGT
GTGTGGCAGTTATTGCTCTCATTTGTTATTGATTTTGTTTTAATTCTTCATTCACTTGAT
GTGACTTCATTTTCAAATGACTCATAGGGCAAAGTTTATTTGTTTGATAAGGTCTTCAAA
CCTAATGCAACTCAAGAAAAAGTATATAGTGAAGCCGCAAAATCTATTGTCGCTGATGTG
CTTGCCGGCTACAATGGAACAATTTTCGCTTATGGTCAAACATCTAGTGGTAAAACACAC
ACAATGGAAGGAGTTATTGGTGATTCAGTTAAGCAGGGCATCATTCCACGAATTGTCAAC
GATATTTTTAATCACATTTATACGATGGAAATGAATTTAGAATTTCACATCAAAGTATCC
TATTATGAAATCTATATGGATAAAATTCGTGATTTATTGGATGTGTCAAAAGTAAATTTG
AGTGTGCACGAAGATAAAAATCGTGTTCCATATGTGAAAGGTGCTACTGAAAGATTTGTA
TCGAGTCCCGAAGATGTCTTTGAAGTTATTGAAGAAGGAAAATCTAATCGTCATATTGCT
GTTACAAATATGAATGAACATTCATCACGTTCACATTCGGTTTTCTTAATTAATGTAAAA
CAGGAAAATCTTGAAAATCAAAAGAAATTATCTGGAAAATTGTACTTGGTTGATTTGGCT
GGTAGTGAAAAAGTATCAAAAACTGGCGCGGAAGGAACAGTGTTAGATGAAGCAAAGAAC
ATTAACAAGTCACTTTCAGCACTTGGTAATGTCATTTCAGCGTTGGCTGATGGCAATAAA
AGTCACATTCCATATCGTGACTCAAAATTGACGAGAATTTTGCAAGAATCACTGGGAGGA
AATGCTCGAACAACAATTGTCATTTGTTGCTCGCCAGCAAGTTTCAATGAGTCGGAAACA
AAATCAACATTGGATTTTGGTAAGCGTGCTAAAACTGTCAAGAATGTTGTATGTGTCAAT
GAGGAACTTACTGCCGAGGAATGGAAACGTCGTTATGAACG
>g12301.t3 Gene=g12301 Length=252
MEGVIGDSVKQGIIPRIVNDIFNHIYTMEMNLEFHIKVSYYEIYMDKIRDLLDVSKVNLS
VHEDKNRVPYVKGATERFVSSPEDVFEVIEEGKSNRHIAVTNMNEHSSRSHSVFLINVKQ
ENLENQKKLSGKLYLVDLAGSEKVSKTGAEGTVLDEAKNINKSLSALGNVISALADGNKS
HIPYRDSKLTRILQESLGGNARTTIVICCSPASFNESETKSTLDFGKRAKTVKNVVCVNE
ELTAEEWKRRYE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g12301.t3 | CDD | cd01369 | KISc_KHC_KIF5 | 1 | 232 | 3.48451E-167 |
| 8 | g12301.t3 | Gene3D | G3DSA:3.40.850.10 | Kinesin | 1 | 252 | 3.6E-114 |
| 2 | g12301.t3 | PANTHER | PTHR24115 | KINESIN-RELATED | 1 | 252 | 6.7E-157 |
| 3 | g12301.t3 | PANTHER | PTHR24115:SF927 | KINESIN FAMILY MEMBER 5A, A-RELATED | 1 | 252 | 6.7E-157 |
| 5 | g12301.t3 | PRINTS | PR00380 | Kinesin heavy chain signature | 101 | 118 | 3.5E-30 |
| 6 | g12301.t3 | PRINTS | PR00380 | Kinesin heavy chain signature | 132 | 150 | 3.5E-30 |
| 4 | g12301.t3 | PRINTS | PR00380 | Kinesin heavy chain signature | 182 | 203 | 3.5E-30 |
| 1 | g12301.t3 | Pfam | PF00225 | Kinesin motor domain | 7 | 232 | 2.1E-84 |
| 10 | g12301.t3 | ProSitePatterns | PS00411 | Kinesin motor domain signature. | 131 | 142 | - |
| 12 | g12301.t3 | ProSiteProfiles | PS50067 | Kinesin motor domain profile. | 1 | 232 | 95.119 |
| 11 | g12301.t3 | SMART | SM00129 | kinesin_4 | 1 | 240 | 8.9E-89 |
| 7 | g12301.t3 | SUPERFAMILY | SSF52540 | P-loop containing nucleoside triphosphate hydrolases | 8 | 247 | 7.44E-89 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:1990939 | NA | NA |
| GO:0007018 | microtubule-based movement | BP |
| GO:0005524 | ATP binding | MF |
| GO:0003777 | microtubule motor activity | MF |
| GO:0008017 | microtubule binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
