General information for g12372.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Ubiquitin-like modifier-activating enzyme 1.
Parent gene
- The parent gene g12372 is located on chr_1: 23142142..23146052 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12372 | g12372.t1 | isoform | g12372.t1 | 23142142 | 23146052 |
| chr_1 | g12372 | g12372.t1 | exon | g12372.t1.exon1 | 23142142 | 23142789 |
| chr_1 | g12372 | g12372.t1 | cds | g12372.t1.CDS1 | 23142142 | 23142789 |
| chr_1 | g12372 | g12372.t1 | exon | g12372.t1.exon2 | 23143458 | 23145442 |
| chr_1 | g12372 | g12372.t1 | cds | g12372.t1.CDS2 | 23143458 | 23145442 |
| chr_1 | g12372 | g12372.t1 | exon | g12372.t1.exon3 | 23145635 | 23146052 |
| chr_1 | g12372 | g12372.t1 | cds | g12372.t1.CDS3 | 23145635 | 23146052 |
| chr_1 | g12372 | g12372.t1 | TSS | g12372.t1 | NA | NA |
| chr_1 | g12372 | g12372.t1 | TTS | g12372.t1 | NA | NA |
Sequences
>g12372.t1 Gene=g12372 Length=3051
ATGCGCGACATTAAAATTGAATCAAAAAGTACTGATGGAACGGCAGTAATTGACGAAAGC
TTGTATTCGCGGCAACTTTACGTCCTCGGTCACGATGCAATGCGAAAAATGCAAAATGCT
GACGTTCTTATCTCGGGTCTTGGCGGTTTAGGCGTTGAAATAGCAAAAAACGTCATTCTT
GGTGGTGTTAGGACTGTTTCACTGCATGATAACAATAATTGCAGTGTCAAAGATTTGTCA
TCGCAATTTTATCTAAACAAAAGTTGCTTAGGCAAAAATCGTGCTGAATGCTGCATCAAA
CAACTCGCGGAATTAAATAATTATGTCACAATAAACCTTGTTACTGAATTATCACAAAAT
ATAATTAGTAAATATCGAGTTGTTGTGCTTACAGAAACTTCAGCTGCTGAACAAAAAAGA
ATTTCAGACATTTGTCGTGAGAATAATGTCGCACTAATTGTGGCAGACACAAAAGGTCTT
TTTGGTCAAATCTTTTGTGATTTTGGTGAGAATTTTATTGTATATGATGATGATGGTATT
CCACCAAAAAGTGGATCAATAATTGGTGTGTCAAAAGAAAGTGAAGGTGTCATAACGACA
GAAAAATGGCACAATCTTTTTGATGGCGATTATGTCACGTTCTCTGGGATTACGGGCATG
ACCGAGTTGAATCAGTGCAAGGCAATGAAAATAAAGAAAATTGGAAACTACTCGTTCAGT
ATTGGCGACACGAGCAACTTTAATGACTACATTGAAGGTGGTACATTCACACAAATAAAG
ATGCCAAAAATGTTGTCCTTCAAATCATTGCGTGAAGCCGAAAATGATGCCAATTTTACA
TTTATGTTGATGGATTTAAATAAATTAGAAAATCCGCAACAAATTCAACTTGCATTCACT
GTTTATCATCGTTTTGTTGAGAAAAATAAACGTGAACCGCAACCATGGAATGAATCAGAT
TCAATGGAATTTTTGCGAATGTGTCTCGATCGTGCTGGTGAATTAAATTGTGAAGTTGAT
GCGAAACTTGTGACAACATTCTCAAAAATTTGCACTAGTCAATTAGCTCCAATAAATGCC
ATTATCGGTGGAATTACTGCACAGGAAGTTATGAAAGCATGCTCAGGCAAATTTATGCCT
ATTTTTCAATATTTTTCATTCGATGCACTTGAATGTTTGCGTGATGAAACAACAATGCCC
AAATATATTTCAAATGGCACTGGATCACGCTATGATTCACAAGCAATAATTTTTGGTGAT
GATGTGCAACGAAAAATTGAAGATCTTCGTTATTTTATTGTTGGTTCAGGTGCAATTGGT
TGTGAATTATTAAAAAATTTTGCAATGATGGGTTTGGGTTGTGGCAATGGTAGTGTTATT
ATAACTGACATGGATTTAATTGAAAAATCAAATTTAAATCGACAATTTCTATTTCGACCT
GAAGATGTTGGTCAACCAAAGTCAAGAACTGCAGCAAGAGCTGTAAAAGCAATGAATCCA
GAACTCAATATTGTCCATCATGAAAATAAAGTTTGTGCCGAAACTGAAGAAATTTATAGT
GAATTTTTTTTTGATAAAATTGATGGTGTTGCAAATGCACTTGATAATGTCGATGCAAGA
CTTTTTATAGATCGAAAATGTGTAATTTATCGCAAGCCTTTAATTGATGCTGGAACATTA
GGTGCAATGGGAAATATTCAATGTATTATTCCATATCTTACAGAATCTTATGCATCATCA
GTTGATCCACCTGAAAAGAATGTTCCTGTTTGTACACTACGACATTTTCCTAATGCCATT
GAGCATACAATTCAATGGGCAAGAGATAAATTTGAAGAACTTTTTGCTGGTGATGCTATA
AATGCTGAAAAATTTTTAACTGATGGAAATTTCCGACAAGAATTGACATCAAAAAGAACA
AAAGCTGCTTTTGAAATGTTGACTAGCATAAAAAAGACACTAATTGAAGATCGTCCATTA
AACTTTAAAGATTGCATTAATTGGGCACGAAATCAATTTGAAGACTTTTTTTCTAATCAA
ATTCGTCAACTTCTTTTCAATTTCCCACATGATCAAATAATGCCAAGTGGTCAACCATTC
TGGTCTGGTTCAAAACGTGCGCCAATGCCAATTAACTTCGACATCAATCAGCGACTGCAC
GTAGATTTTATCATATCGGCTGCCAATTTGAGAGCAGACATGTTTGGCATTGAGCAGAAT
CGCGATTATTCATATGCCATTGAATGTGTGCGTGAGGTCATTGTACCTGAATTTGAACCA
AAGAGTGGAGTAAAAATACCGGTGAATGATTCGGAAATTGAGGAAGAGAAAGCCGAGGAT
GGTGAGGAAGAAATTGATGAAATTGTGATTAAGCAATTGTGCGATGAACTAACGAGATTA
GATGGACATTCATTCAATTTCAAAGCACTGAGATTTGAAAAGGATGATGATGAAAATTTA
CATATGGATTTTGTTACTGCGGCCAGTAATGTGCGTGCATTGAATTATAAAATTGAGCCA
ATTGATAAATTCAGGACAAAGATGATTGCAGGCAAAATAATTGCGGCTATTGCAACTTGT
ACTTCAGTTGTTGCTGGCTTTGCTTGTTTGGAAATTTATAAAATTGCTCAAGGCTTCAAT
GACATCACAAAATACCAAAATCGTTATTTCAATTTAGCTGACAACACATATGCTTTCTCA
GAGCCAAAACCCGCACAAATCAGCAAATTTTATAACACAAATTACACAATTTGGGATCGC
TTTGAAATTAATCGTCCAATGACCCTTCGAGAGCTTCTCGATTATTTCAAAAAGGAACAT
CGACTGGAAATTACAATGCTTTCACAAAACATTACTTTGCTCTACTCGTTTTTCATGTCG
GAAAAGAAACTGAAGGAACGTGAACACTTACACATTGACAAGTGCATTGAGCACATAATG
GAGGAGAAAATTGGAGAGCACATTAAATCATTGATATTGGAAGCTTATTGTGTCGATGAG
GATGACGTTGAAGTGGAAGTTCCGTACATCAGATATCTTCTCAAGTCGTAA
>g12372.t1 Gene=g12372 Length=1016
MRDIKIESKSTDGTAVIDESLYSRQLYVLGHDAMRKMQNADVLISGLGGLGVEIAKNVIL
GGVRTVSLHDNNNCSVKDLSSQFYLNKSCLGKNRAECCIKQLAELNNYVTINLVTELSQN
IISKYRVVVLTETSAAEQKRISDICRENNVALIVADTKGLFGQIFCDFGENFIVYDDDGI
PPKSGSIIGVSKESEGVITTEKWHNLFDGDYVTFSGITGMTELNQCKAMKIKKIGNYSFS
IGDTSNFNDYIEGGTFTQIKMPKMLSFKSLREAENDANFTFMLMDLNKLENPQQIQLAFT
VYHRFVEKNKREPQPWNESDSMEFLRMCLDRAGELNCEVDAKLVTTFSKICTSQLAPINA
IIGGITAQEVMKACSGKFMPIFQYFSFDALECLRDETTMPKYISNGTGSRYDSQAIIFGD
DVQRKIEDLRYFIVGSGAIGCELLKNFAMMGLGCGNGSVIITDMDLIEKSNLNRQFLFRP
EDVGQPKSRTAARAVKAMNPELNIVHHENKVCAETEEIYSEFFFDKIDGVANALDNVDAR
LFIDRKCVIYRKPLIDAGTLGAMGNIQCIIPYLTESYASSVDPPEKNVPVCTLRHFPNAI
EHTIQWARDKFEELFAGDAINAEKFLTDGNFRQELTSKRTKAAFEMLTSIKKTLIEDRPL
NFKDCINWARNQFEDFFSNQIRQLLFNFPHDQIMPSGQPFWSGSKRAPMPINFDINQRLH
VDFIISAANLRADMFGIEQNRDYSYAIECVREVIVPEFEPKSGVKIPVNDSEIEEEKAED
GEEEIDEIVIKQLCDELTRLDGHSFNFKALRFEKDDDENLHMDFVTAASNVRALNYKIEP
IDKFRTKMIAGKIIAAIATCTSVVAGFACLEIYKIAQGFNDITKYQNRYFNLADNTYAFS
EPKPAQISKFYNTNYTIWDRFEINRPMTLRELLDYFKKEHRLEITMLSQNITLLYSFFMS
EKKLKEREHLHIDKCIEHIMEEKIGEHIKSLILEAYCVDEDDVEVEVPYIRYLLKS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 22 | g12372.t1 | CDD | cd01491 | Ube1_repeat1 | 21 | 396 | 2.14054E-129 |
| 23 | g12372.t1 | CDD | cd01490 | Ube1_repeat2 | 430 | 967 | 0.0 |
| 19 | g12372.t1 | Gene3D | G3DSA:3.50.50.80 | - | 15 | 129 | 3.7E-41 |
| 18 | g12372.t1 | Gene3D | G3DSA:3.40.50.12550 | - | 139 | 421 | 1.3E-97 |
| 17 | g12372.t1 | Gene3D | G3DSA:2.40.30.180 | - | 181 | 259 | 1.3E-97 |
| 16 | g12372.t1 | Gene3D | G3DSA:3.40.50.720 | - | 428 | 905 | 5.8E-193 |
| 20 | g12372.t1 | Gene3D | G3DSA:1.10.10.2660 | - | 582 | 854 | 5.8E-193 |
| 21 | g12372.t1 | Gene3D | G3DSA:3.10.290.60 | - | 920 | 1014 | 3.4E-31 |
| 7 | g12372.t1 | PANTHER | PTHR10953:SF195 | UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME 1 | 14 | 1009 | 0.0 |
| 8 | g12372.t1 | PANTHER | PTHR10953 | UBIQUITIN-ACTIVATING ENZYME E1 | 14 | 1009 | 0.0 |
| 11 | g12372.t1 | PRINTS | PR01849 | Ubiquitin-activating enzyme E1 signature | 43 | 67 | 2.4E-60 |
| 9 | g12372.t1 | PRINTS | PR01849 | Ubiquitin-activating enzyme E1 signature | 155 | 182 | 2.4E-60 |
| 12 | g12372.t1 | PRINTS | PR01849 | Ubiquitin-activating enzyme E1 signature | 457 | 480 | 2.4E-60 |
| 10 | g12372.t1 | PRINTS | PR01849 | Ubiquitin-activating enzyme E1 signature | 504 | 529 | 2.4E-60 |
| 13 | g12372.t1 | PRINTS | PR01849 | Ubiquitin-activating enzyme E1 signature | 657 | 684 | 2.4E-60 |
| 5 | g12372.t1 | Pfam | PF00899 | ThiF family | 22 | 396 | 7.5E-32 |
| 1 | g12372.t1 | Pfam | PF16190 | Ubiquitin-activating enzyme E1 FCCH domain | 196 | 261 | 9.2E-25 |
| 3 | g12372.t1 | Pfam | PF16191 | Ubiquitin-activating enzyme E1 four-helix bundle | 262 | 327 | 3.4E-13 |
| 4 | g12372.t1 | Pfam | PF00899 | ThiF family | 411 | 906 | 1.3E-68 |
| 6 | g12372.t1 | Pfam | PF10585 | Ubiquitin-activating enzyme active site | 597 | 847 | 4.3E-86 |
| 2 | g12372.t1 | Pfam | PF09358 | Ubiquitin fold domain | 918 | 1010 | 2.3E-26 |
| 25 | g12372.t1 | ProSitePatterns | PS00536 | Ubiquitin-activating enzyme signature 1. | 372 | 380 | - |
| 24 | g12372.t1 | ProSitePatterns | PS00865 | Ubiquitin-activating enzyme active site. | 589 | 597 | - |
| 26 | g12372.t1 | SMART | SM00985 | UBA_e1_C_a_2 | 885 | 1010 | 1.2E-43 |
| 14 | g12372.t1 | SUPERFAMILY | SSF69572 | Activating enzymes of the ubiquitin-like proteins | 17 | 393 | 6.54E-94 |
| 15 | g12372.t1 | SUPERFAMILY | SSF69572 | Activating enzymes of the ubiquitin-like proteins | 408 | 901 | 2.75E-128 |
| 27 | g12372.t1 | TIGRFAM | TIGR01408 | Ube1: ubiquitin-activating enzyme E1 | 17 | 1013 | 0.0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008641 | ubiquitin-like modifier activating enzyme activity | MF |
| GO:0006464 | cellular protein modification process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed