General information for g12527.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Aminopeptidase N.
Parent gene
- The parent gene g12527 is located on chr_1: 24251772..24255140 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12527 | g12527.t2 | isoform | g12527.t2 | 24251772 | 24252899 |
| chr_1 | g12527 | g12527.t2 | exon | g12527.t2.exon1 | 24251772 | 24252461 |
| chr_1 | g12527 | g12527.t2 | cds | g12527.t2.CDS1 | 24251872 | 24252461 |
| chr_1 | g12527 | g12527.t2 | exon | g12527.t2.exon2 | 24252527 | 24252899 |
| chr_1 | g12527 | g12527.t2 | cds | g12527.t2.CDS2 | 24252527 | 24252899 |
| chr_1 | g12527 | g12527.t2 | TSS | g12527.t2 | NA | NA |
| chr_1 | g12527 | g12527.t2 | TTS | g12527.t2 | NA | NA |
Sequences
>g12527.t2 Gene=g12527 Length=1063
ATGATAAGAATTATCAGATTACCTGATTGCTATTTAAGTGAGTGAAAATTCATAAGTATC
ACTCAGTTGCGTGTGGATTTTTTTAGGAAATAAAAAAATCATGAAAGTTCTTTTTTACAT
CGTTCTCATAGTGATAATCGGGAATGTAGAGTTGAAAAAAACACCACCTTATATAGCAAG
AAAATTAGCTTTGCAAGAATCCGGCTTTAGTCTTGCTAGACCAACGTCAGACGTTGAGGC
TCAAGCAGATGTCATCAATTATCGCTTACCAAACAATACTAAACCATTGTATTATGACAT
TCATATGACAACTCATATTCATGAAAATAAATTTGAGTTTAATGGTCATGTGATTATAAA
GTTTGAAGTTAAAACTTTACCATTAAATGAAATTGTAATTCATATGAAAGAATTAGAAAA
TCTTGAAATAAAACTTTTAAATTCAACTCTTGGATTGATTGAAAATAATGTGGATTATGA
TCATGAAGAAGAAACTGATTTTCTCATAATTAAAACAAAAAATAATCTGGTTTTAAATGG
AATTTATCATCTTGATATAACATATGATGGTGAATTAAGAGACGACAATTTGGGTTTTTA
TAGATCTTCATATTTAAATGACAAGAATGAAAATATTTGGCTAGCAACGACACAATTTGA
AAGTGTAGAAGCAAGAAGTGCTTTCCCATGCTATGATGAACCGCAAATTCGCGCACCTTT
TAAAATTAAGATAACATATGGAAAAAATTACAAAGCTGTTTCAAACATGCCAGTAATAGA
AACAATCGATGAGAGTAGCTTACAGGGCTACGTGACCACATCATTTAAAGAAAGTCCTCC
CATACAAACATATCTGATTGCTTTCATCATTTCTGACTTTGATTATGTGTCGAAAGGTGT
ACAAAAAGTTTATGCAAAACCCAATTCTATTAAAGATGGTGAAGCTGATCTTGCATTACG
TTATGGAATAGAAATTATAGCTAAATTCGAAGAGTATTTGGGTGTTGATTATTTTACAGA
GAAAATGGATCAATTTGCCCTACCCGATTTTGATGCTGGTGCT
>g12527.t2 Gene=g12527 Length=321
MKVLFYIVLIVIIGNVELKKTPPYIARKLALQESGFSLARPTSDVEAQADVINYRLPNNT
KPLYYDIHMTTHIHENKFEFNGHVIIKFEVKTLPLNEIVIHMKELENLEIKLLNSTLGLI
ENNVDYDHEEETDFLIIKTKNNLVLNGIYHLDITYDGELRDDNLGFYRSSYLNDKNENIW
LATTQFESVEARSAFPCYDEPQIRAPFKIKITYGKNYKAVSNMPVIETIDESSLQGYVTT
SFKESPPIQTYLIAFIISDFDYVSKGVQKVYAKPNSIKDGEADLALRYGIEIIAKFEEYL
GVDYFTEKMDQFALPDFDAGA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g12527.t2 | Gene3D | G3DSA:2.60.40.1730 | tricorn interacting facor f3 domain | 44 | 259 | 2.0E-62 |
| 10 | g12527.t2 | Gene3D | G3DSA:1.10.390.60 | - | 263 | 321 | 4.5E-9 |
| 2 | g12527.t2 | PANTHER | PTHR11533:SF283 | AMINOPEPTIDASE | 41 | 321 | 2.6E-76 |
| 3 | g12527.t2 | PANTHER | PTHR11533 | PROTEASE M1 ZINC METALLOPROTEASE | 41 | 321 | 2.6E-76 |
| 6 | g12527.t2 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 192 | 207 | 7.6E-11 |
| 4 | g12527.t2 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 242 | 257 | 7.6E-11 |
| 5 | g12527.t2 | PRINTS | PR00756 | Membrane alanyl dipeptidase (M1) family signature | 317 | 321 | 7.6E-11 |
| 1 | g12527.t2 | Pfam | PF17900 | Peptidase M1 N-terminal domain | 61 | 252 | 1.4E-44 |
| 12 | g12527.t2 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 13 | g12527.t2 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 14 | g12527.t2 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 13 | - |
| 15 | g12527.t2 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 18 | - |
| 11 | g12527.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 321 | - |
| 7 | g12527.t2 | SUPERFAMILY | SSF63737 | Leukotriene A4 hydrolase N-terminal domain | 48 | 259 | 1.12E-52 |
| 8 | g12527.t2 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed