General information for g12641.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Serine protease inhibitor 77Ba.
Parent gene
- The parent gene g12641 is located on chr_1: 24968260..24971610 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12641 | g12641.t1 | TSS | g12641.t1 | 24968083 | 24968083 |
| chr_1 | g12641 | g12641.t1 | isoform | g12641.t1 | 24968260 | 24971610 |
| chr_1 | g12641 | g12641.t1 | exon | g12641.t1.exon1 | 24968260 | 24968500 |
| chr_1 | g12641 | g12641.t1 | cds | g12641.t1.CDS1 | 24968260 | 24968500 |
| chr_1 | g12641 | g12641.t1 | exon | g12641.t1.exon2 | 24968558 | 24969163 |
| chr_1 | g12641 | g12641.t1 | cds | g12641.t1.CDS2 | 24968558 | 24969163 |
| chr_1 | g12641 | g12641.t1 | exon | g12641.t1.exon3 | 24969225 | 24969274 |
| chr_1 | g12641 | g12641.t1 | cds | g12641.t1.CDS3 | 24969225 | 24969274 |
| chr_1 | g12641 | g12641.t1 | exon | g12641.t1.exon4 | 24970017 | 24970330 |
| chr_1 | g12641 | g12641.t1 | cds | g12641.t1.CDS4 | 24970017 | 24970330 |
| chr_1 | g12641 | g12641.t1 | exon | g12641.t1.exon5 | 24970398 | 24970626 |
| chr_1 | g12641 | g12641.t1 | cds | g12641.t1.CDS5 | 24970398 | 24970626 |
| chr_1 | g12641 | g12641.t1 | exon | g12641.t1.exon6 | 24970686 | 24971141 |
| chr_1 | g12641 | g12641.t1 | cds | g12641.t1.CDS6 | 24970686 | 24971141 |
| chr_1 | g12641 | g12641.t1 | exon | g12641.t1.exon7 | 24971198 | 24971356 |
| chr_1 | g12641 | g12641.t1 | cds | g12641.t1.CDS7 | 24971198 | 24971356 |
| chr_1 | g12641 | g12641.t1 | exon | g12641.t1.exon8 | 24971515 | 24971610 |
| chr_1 | g12641 | g12641.t1 | cds | g12641.t1.CDS8 | 24971515 | 24971610 |
| chr_1 | g12641 | g12641.t1 | TTS | g12641.t1 | NA | NA |
Sequences
>g12641.t1 Gene=g12641 Length=2151
ATGAAGGGAAATTTTTACTTCATTTTCTTATTATTATTTTTTGTGAAGTCTTCAGTATTT
TCAATTCGACGTATAAATTCTTGTGGTGTTTCGAAATTTGAAAGAAATAAAAGTTTTATT
GGGCTTGTAGTTAATGGTGATGAAAGTAAACCAGGTGAATGGCCATGGATTGTTTCAATG
TTTAGAAAAGAAAAATATTTTTGTGGTTCATCTTTGATTTCTGATTTGCATTTGTTATCT
GCTGCTCATTGTTTCGAATATTTTGGAATCACTTTTCAACTTAACGATTATTTTGCTCTT
CTTGGTCGATTCAATTTGAAAGACAACAACGAGAAGTTTTCAGTCAATCGAAGTTTTTCT
TCAATTTTTCTTCATTCTGAGTTTAATAATACAGCTGAAGCTTATCGTAGCAATGCTGAC
ATTGCAGTTATTCAAATGTCAGAAAGAGTTCAATTTTCAGATTTTATACAACCAGTTTGT
CTTCCTGAGCCAAATTCTAATTTAGAAAATTTTGATGGAATTGTTGTTGGTTATGGCAAA
AGTGAGTCTGGTGAAATTCATGAAGTGACACCAAAGCAAGCTGAATTGCATTCAATTAAT
GTTTTTGAGTGTTTATTAAAAGATCCTCTGTATAGTTACATTGTGGCTGAAAGAAGTTTT
TGTGCAGGTGGCACTGATGCGATTCCATGTTTGGGAGATTCTGGTGGAGGATTTTATGTA
AAAAATAACAAAAGTGGAAAATACGAAACGAAAGGAATTGTGTCACAAGCACAACATAAT
GGTTGTGATCCAAAAGTTTATGTTGCTTTCGTTGATGTTACCAAGTTTATTGACTGGATT
ATTGAAAAAATGAATGAAATATCTAAACCAGAAGAAAAGAAACGCATTAAAACTGAAATG
AGCTCTGCTGATCGCAAAACAACAAAATTACCTCTTATTTTGCCTACTTCTGTAGATACT
AATATTCGAAAATTATTATGGGATCAAGAATTGTCAGAAAATACTGAACTATTTGGATTG
CACTTATTTTTGTATTTGACTCAATTTGAGTCTGAAAATTTCATGATTAATCCATATTTA
ATTCATTCACTTTTAGCAGTCCTTGCTGAAGGTGCAGTTGGTAATACATATAAAGAAATA
AACAATGCTCTTGGTTTAATAAATAGACAACGAACAAGAGATTTCCATCAATATACTAAC
TTAGCTTTAAGTAAAAGTGCGTCAGATGTGAACTTTCGTAAATTTGCTGCTATGATAGGT
GATCAAAATCGACCGATCACACGCGAATATGAAGACAATTTAGAAAAAATTTATGATGTA
GAATACATTCCTGTAAATTTCAAAAATGTAGACAGAACTTTACGTGAAGTAAATGGCAGA
GTTTCACAAAGTACAAGTGGGTTAATAACTGACGTCATCTCACGTGAAGATGTACTTAAG
ACTCAGTTAATACTGTTAGCATGTACTTATTTCAAAGGAAGCTGGAAAATTGCCTTCAAT
TCGACATTAACAAAGTATGAACCATTTTATGATATTAATGAGGAGAGAATAATTGGAAGA
GTTAATATGATGTCTCAAAGTGGTTCATTTGCTTATGCTTTTAATCAAAATCTTGGCTGT
TATTTTCTTGAATTACCATATGGAATCGACCGTGAATATGCAAAAAGAATTAATTTGCCA
GAAAGCGCTGAAGATCGAATTTCAATGATTGTTGTTTTACCAAAACGTGGTCTTTCATTG
ATTGATGTCATTAATAACATTAGCGTTTATGGAATAAAGACCTTGCTAATAGAATTGAAA
AAAAGTAAAGAAGAAAATAAAAACTTAGAAGTTGAAGTTCATCTTCCACGTTTTGAAATA
AACACATCACTCAATTTAAAGGAGACTTTAAAAGATTTGGAAATAAATGAGATTTTTGAG
AATACAGCAAATTTGTCAGGAATCAATTATAACTACTATGTTTCGTCGATTCTTCACAAG
ACAAAAATTAATGTAGATGAAAAAGGATCTGAAGCACCAACAGTGACAAATTTAATTTCT
GCTAACAAAGTCAAGTTCTATGCTAACAGACCTTTTATTTACTTTATTCTTGACAAAGCC
ACGAGACTCATTCTTTTTGCTGGCGTGTTTAAAAATCCTGCAGTTTTTTAA
>g12641.t1 Gene=g12641 Length=716
MKGNFYFIFLLLFFVKSSVFSIRRINSCGVSKFERNKSFIGLVVNGDESKPGEWPWIVSM
FRKEKYFCGSSLISDLHLLSAAHCFEYFGITFQLNDYFALLGRFNLKDNNEKFSVNRSFS
SIFLHSEFNNTAEAYRSNADIAVIQMSERVQFSDFIQPVCLPEPNSNLENFDGIVVGYGK
SESGEIHEVTPKQAELHSINVFECLLKDPLYSYIVAERSFCAGGTDAIPCLGDSGGGFYV
KNNKSGKYETKGIVSQAQHNGCDPKVYVAFVDVTKFIDWIIEKMNEISKPEEKKRIKTEM
SSADRKTTKLPLILPTSVDTNIRKLLWDQELSENTELFGLHLFLYLTQFESENFMINPYL
IHSLLAVLAEGAVGNTYKEINNALGLINRQRTRDFHQYTNLALSKSASDVNFRKFAAMIG
DQNRPITREYEDNLEKIYDVEYIPVNFKNVDRTLREVNGRVSQSTSGLITDVISREDVLK
TQLILLACTYFKGSWKIAFNSTLTKYEPFYDINEERIIGRVNMMSQSGSFAYAFNQNLGC
YFLELPYGIDREYAKRINLPESAEDRISMIVVLPKRGLSLIDVINNISVYGIKTLLIELK
KSKEENKNLEVEVHLPRFEINTSLNLKETLKDLEINEIFENTANLSGINYNYYVSSILHK
TKINVDEKGSEAPTVTNLISANKVKFYANRPFIYFILDKATRLILFAGVFKNPAVF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 21 | g12641.t1 | CDD | cd00190 | Tryp_SPc | 43 | 280 | 3.38118E-49 |
| 22 | g12641.t1 | CDD | cd19598 | serpin77Ba-like_insects | 331 | 713 | 1.61739E-131 |
| 15 | g12641.t1 | Coils | Coil | Coil | 592 | 612 | - |
| 13 | g12641.t1 | Gene3D | G3DSA:2.40.10.10 | - | 45 | 274 | 8.1E-50 |
| 14 | g12641.t1 | Gene3D | G3DSA:2.40.10.10 | - | 55 | 280 | 8.1E-50 |
| 11 | g12641.t1 | Gene3D | G3DSA:2.30.39.10 | - | 351 | 713 | 1.6E-83 |
| 12 | g12641.t1 | Gene3D | G3DSA:3.30.497.10 | Antithrombin | 358 | 669 | 1.6E-83 |
| 3 | g12641.t1 | PANTHER | PTHR11461:SF162 | SERINE PROTEASE INHIBITOR 77BA | 331 | 714 | 6.9E-81 |
| 4 | g12641.t1 | PANTHER | PTHR11461 | SERINE PROTEASE INHIBITOR, SERPIN | 331 | 714 | 6.9E-81 |
| 7 | g12641.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 69 | 84 | 1.8E-5 |
| 6 | g12641.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 136 | 150 | 1.8E-5 |
| 5 | g12641.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 227 | 239 | 1.8E-5 |
| 1 | g12641.t1 | Pfam | PF00089 | Trypsin | 43 | 280 | 2.5E-38 |
| 2 | g12641.t1 | Pfam | PF00079 | Serpin (serine protease inhibitor) | 337 | 713 | 2.5E-79 |
| 17 | g12641.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 21 | - |
| 18 | g12641.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 19 | g12641.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 15 | - |
| 20 | g12641.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 21 | - |
| 16 | g12641.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 22 | 716 | - |
| 25 | g12641.t1 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 79 | 84 | - |
| 26 | g12641.t1 | ProSitePatterns | PS00284 | Serpins signature. | 686 | 696 | - |
| 27 | g12641.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 43 | 285 | 23.9 |
| 23 | g12641.t1 | SMART | SM00020 | trypsin_2 | 42 | 280 | 1.2E-45 |
| 24 | g12641.t1 | SMART | SM00093 | serpin2 | 340 | 713 | 4.2E-52 |
| 8 | g12641.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 7 | 285 | 1.67E-58 |
| 9 | g12641.t1 | SUPERFAMILY | SSF56574 | Serpins | 320 | 713 | 3.14E-82 |
| 10 | g12641.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0005615 | extracellular space | CC |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
- This transcript has been annotated with K04525 (serpin A)
Pathway
This gene does not belong to any pathways.
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed