General information for g12710.t7
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Translocon-associated protein subunit alpha.
Parent gene
- The parent gene g12710 is located on chr_1: 25414443..25417609 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12710 | g12710.t7 | isoform | g12710.t7 | 25414034 | 25415531 |
| chr_1 | g12710 | g12710.t7 | exon | g12710.t7.exon1 | 25414034 | 25415043 |
| chr_1 | g12710 | g12710.t7 | TTS | g12710.t7 | 25414039 | 25414039 |
| chr_1 | g12710 | g12710.t7 | cds | g12710.t7.CDS1 | 25414443 | 25415043 |
| chr_1 | g12710 | g12710.t7 | exon | g12710.t7.exon2 | 25415101 | 25415298 |
| chr_1 | g12710 | g12710.t7 | cds | g12710.t7.CDS2 | 25415101 | 25415298 |
| chr_1 | g12710 | g12710.t7 | exon | g12710.t7.exon3 | 25415488 | 25415531 |
| chr_1 | g12710 | g12710.t7 | cds | g12710.t7.CDS3 | 25415488 | 25415531 |
| chr_1 | g12710 | g12710.t7 | TSS | g12710.t7 | 25415638 | 25415638 |
Sequences
>g12710.t7 Gene=g12710 Length=1252
ATGAAGCATTCATTGCTATTAGTTCTTCTCATTTTACCTGCATTAGCAAATGAAGACGAA
ATTGATGAGGATTTAGTTGATGTCGAAAATGAAGGTGATGCAGATGAAGCAAGTGAAAGT
GCGCTGACTGGCGATGATGTGGAGGCAGATGAGACTGGTGAAAGTACGAAATCTCCTGAT
GCTGACACTCAATTGCTCTTTGTTCGACCACTTTATACACCAGGATCACAACTTGAATTA
CCTGGCGGTGTTCCAGTAGAGTTCTTAATTGGCTTCTTAAATAAAGGCAAAAATGATTTC
ATTATTGAGAGTGTTGAAGCTTCATTCCGTTATCCAATGGATTTCACTTACTACATTCAA
AATTTCTCAGCACTTGCATATAACCGTGAAGTTAAATCAGAGAGTGAAGCAACTGTATCA
TACTCATTCCTTCCATCAGAACAATTTGCTGGTCGTCCTTTTGGTTTGAATATTGCCATT
AGTTATCGTGATTCAACTGGTGCAGGTTTTATGGAAGCAGTTTTCAATGAAACTGTTCTC
ATCACAGAATTAGATGAAGGTCTTGATGGCGAAACATTCTTTTTATATGTGTTCTTTGCA
GCTATTTTTGTTTTACTTCTTGTTGTTGGACAACAATTCCTCGGTTCAATTGGCAAGAAG
AAACGTTCACAAAACATTCGTAAGCAAGTTGAGACTGGTACATCAAATAACACCGATATT
GATTATGAATGGCTTCCTGCTCAAACAATTAGAAATTTACAAAATTCACCAAATGGCAAA
GGAAAAATCTCGCCAAAACAGAGTCCAAGACAACGTTCAAAACGTGCTGCAAAGGAAGAT
TAAGAAACGAATTTTTAAAGAGTTGTTAATTTCATCCCCTCTATGAGATTCATACCTATG
AGCAAGTCAAGCTTCTCTAGAAATCCAATAAAACACATAAACCACATAAATTTTAATAGA
ACAACTTAAAAATTCATCGTAATACTTACCCTATGTTGTCTTCAAATCATTATGCATGGA
GCATAATTAAAACTTTTTTTTTGATCTTGCAATAGTTGATTCATAGAGTATAAGTGTTTT
GCGTTTAAGATTCCAATTCTTATCTTTTTGTTGCAAGATCACACCAATCATTATCATTTT
ATAATTGAAAAGAAAAAGAGTAAAAAGAGATCATCAATGATGTTTGTTCTGAAAAAGATG
AGAAGAACTTAATAATAAAATTTAATTAAAAAATTGAAAAAGAACTGAAAAA
>g12710.t7 Gene=g12710 Length=280
MKHSLLLVLLILPALANEDEIDEDLVDVENEGDADEASESALTGDDVEADETGESTKSPD
ADTQLLFVRPLYTPGSQLELPGGVPVEFLIGFLNKGKNDFIIESVEASFRYPMDFTYYIQ
NFSALAYNREVKSESEATVSYSFLPSEQFAGRPFGLNIAISYRDSTGAGFMEAVFNETVL
ITELDEGLDGETFFLYVFFAAIFVLLLVVGQQFLGSIGKKKRSQNIRKQVETGTSNNTDI
DYEWLPAQTIRNLQNSPNGKGKISPKQSPRQRSKRAAKED
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g12710.t7 | MobiDBLite | mobidb-lite | consensus disorder prediction | 30 | 49 | - |
| 7 | g12710.t7 | MobiDBLite | mobidb-lite | consensus disorder prediction | 30 | 60 | - |
| 5 | g12710.t7 | MobiDBLite | mobidb-lite | consensus disorder prediction | 251 | 266 | - |
| 8 | g12710.t7 | MobiDBLite | mobidb-lite | consensus disorder prediction | 251 | 280 | - |
| 2 | g12710.t7 | PANTHER | PTHR12924:SF0 | TRANSLOCON-ASSOCIATED PROTEIN SUBUNIT ALPHA | 5 | 273 | 4.4E-69 |
| 3 | g12710.t7 | PANTHER | PTHR12924 | TRANSLOCON-ASSOCIATED PROTEIN, ALPHA SUBUNIT | 5 | 273 | 4.4E-69 |
| 1 | g12710.t7 | Pfam | PF03896 | Translocon-associated protein (TRAP), alpha subunit | 14 | 279 | 2.1E-110 |
| 12 | g12710.t7 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 13 | g12710.t7 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 14 | g12710.t7 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 12 | - |
| 16 | g12710.t7 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 16 | - |
| 11 | g12710.t7 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 192 | - |
| 15 | g12710.t7 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 193 | 214 | - |
| 10 | g12710.t7 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 215 | 280 | - |
| 9 | g12710.t7 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 16 | - |
| 4 | g12710.t7 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 196 | 218 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005789 | endoplasmic reticulum membrane | CC |
KEGG
Orthology
- This transcript has been annotated with K13249 (translocon-associated protein subunit alpha)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed