General information for g12745.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Beta-1,4-mannosyltransferase egh.
Parent gene
- The parent gene g12745 is located on chr_1: 25674322..25675957 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12745 | g12745.t3 | TSS | g12745.t3 | 25669523 | 25669523 |
| chr_1 | g12745 | g12745.t3 | isoform | g12745.t3 | 25669679 | 25675957 |
| chr_1 | g12745 | g12745.t3 | exon | g12745.t3.exon1 | 25669679 | 25669691 |
| chr_1 | g12745 | g12745.t3 | exon | g12745.t3.exon2 | 25672532 | 25673650 |
| chr_1 | g12745 | g12745.t3 | cds | g12745.t3.CDS1 | 25672847 | 25673650 |
| chr_1 | g12745 | g12745.t3 | exon | g12745.t3.exon3 | 25673732 | 25675957 |
| chr_1 | g12745 | g12745.t3 | cds | g12745.t3.CDS2 | 25673732 | 25674322 |
| chr_1 | g12745 | g12745.t3 | TTS | g12745.t3 | NA | NA |
Sequences
>g12745.t3 Gene=g12745 Length=3358
TCTAAAAAAATATACTCCACTAAACAAACGAATAGATAAAAGACTATAATTATTGGAGAA
ACAGTTTTTAATGCTTCAGACAAGTTGACAAAGTGAAAGAGCAAGAAAAACGTCATCATC
AAATAATGTCTCTTTAGATCAATTCATGCATATTATTTTAATATTTCTTCATCTCAAGAA
ATATTGACGACAACTAAAAGTCGGAATTAATTGGAGTTCATCAAAATAATTTTCTTCTTT
CTATTAGTGCTACACATCAATTTGAACGTAAACTGATTTAAAAAAAATAACAAGATTTTA
ATAAATAGTACAAGTTGAATTCAAGAAAATGTTAACATCAACTGCAAAGCACATTCTTCA
CTGCATCGCTCTCGGCGTGGTCATCACATTCTTTCTTTTTTCCTCTGGTGCTATAAAGAC
GAGTGACGATGATTATTTGGCAATCGACCCTTGGGAAGAGTATGGAACGTTTATGACAAT
TTTTCTTTATACCATTCGTAGTATATCTATATTGATACTGCCACAAATGCTTTGCAATTT
TCTTGGGCTTGTTTGCTTCAATGCTTTCCCTGACAAAGTTGTTCTCAAAGGATCACCACT
TCTGGCACCATTCATTTGCATACGAGTCGTTACTAGAGGTGATTTTGCCGACCTTGTGAA
GGCCAATGTTATGAGAAATATGAATACATGTTTAGACACGGGACTTGAGAATTTTCTTAT
AGAAGTTGTTACCGATAAGGCAGTAAATCTTCCGAAACATCGTCGAATTAGAGAGATTGT
GGTACCGAAAGATTATAAAACAAAATCTGGTGCATTATTTAAAGCACGTGCTCTTCAATA
TTGCTTAGAAGACAATGTTAATGTATTAAATCCAAATGACTGGATCGTTCACTTAGATGA
AGAGACCATACTTACAGAAAATAGTGTTCGAGGCATCACAAATTTTGTTTTAGATGGCAA
ACATCCTTTTGGTCAAGGATTAATTACATACGCAAATGAAAGTATTGTCAACATTTTTAC
AACACTTGCAGACAGCTTCCGAGTATCTGATGATATGGGAAAATTGAGATTTCAGTTTAA
AGTTTTTCATAAACCACCATTTAGTTGGAAAGGCAGTTATGTTGTTACACAGCTACATGC
AGAAAAAGATGTTTCATTTGATAATGGTATGGATGGATCAGTTGCAGAAGATTGTTATTT
TGCGATGAAAGCATTCAGCAGAGGATACACTTTTGATTTTATTGATGGTGAAATGTATGA
AAAATCACCGTTTACAATACTCGATTTTATTCAACAACGCAAACGTTGGCTTCAGGGAAT
TTTGTTGGTAATTCATTCAAAAGCCATTCCATTTAAATATAAATCGTTCCTCGCACTAAG
TGTCTACAGCTGGATTATGATGCCACTTTCATCATCAAATATTTTTTTCGCCATCTACAT
GCCGATTCCATGTCCATTCATATTAGACCTTATTTGTTCTTTTCTCGGTGGAATTAGTGT
CTATATGTACGTTTATGGTGTTTATAAATCATTTCCATTAAAACGCATTGGATTCTTTAA
GTCAGTTTTGTACATTATTGGAGCACTCGCTATGATACCATTTAATTTAGTAATTGAAAA
TATCGCTGTGATGTGGGGTGTATTGAGCAATAAGCATAGATTTTATGTTGTACAGAAAGA
AGTATCAATGACAACAGAACAACGTCCATTAATGACAGTTTAAATATATGTGTTTATTAA
TTTTTTAGTCAAACATGAGAAAATTGTTAGATCAAAACAAATAAGAAGTGTCGAATTAAT
TTATAAAATGATCAAAGAGCAACCGTCATAAGATTTTTTTCACTTTTTATACGCCTCCAA
ATATTGAGAAAAAAAATTAACTTCAATATTTAAAACGCTTGTTTGTAGAAAAAGTTTACG
TAAATTTGTTTAAGTGCGCGATTAAAATATTTTTCTACTGTTTTAGGTCCATATAATTTT
TGCATTTCCATCTTCATTATCTAATTTAAACCAATCTTTTTTCTTTTGTCGTTTTCGTAT
CATGAGAAAAAATAAATTTTTGCACAAAAAGAAATTATGTACATGAATTTTTGAAGACAT
CAATCAAGAACATTAGAGTCATACTTATTAAAAGAAAGCAATACATCATTCAATATTCAT
TCAAATTATCACATAAAAACTTTAAGAATAATTTACATACACACACATATGAGAAACATG
TGATAATATGTAAAATTTTTAATTGAAAAAGATTTGAAGGAAAATAAGGCGTTTCATAAG
AACCAAGAACATTCGTATTTCAAGATCAAGTAAAATGAATAATAAAAATAAAATTAAAAG
TAGTTTAAAGTGCTTTAAAAATGCAAAAAAAAATGAAGAAAGCACGATTGATACCAAATT
TTTTATTTTGTTTATCAAACAAAGTAAATTTAGATGAATAAGCTGAAAAATTTTTATTAA
ATAAAAAGAAAAACTTATGAGTTAAAAACCCACCATTAAAATTCTCAAATTCATCTTCAA
GACATTTTGAGATCAATTGAGAAATTAATAAACAACCAATCGGTGCGATTATTACATTAA
TTTAAATACTTTAAAAAGTACTATCTACATACTAATTAGAAATTTGAAATATATTACCTA
CTTTAAAGACAGAATAATTTATATGTGTAAATCCTGTCTTGATTTCTATTGTTTTGAATT
TTTATATAATGAGGCTACTATAAAGCTTAAATAAGAACAGAAACGATCAGTTGTATTAAT
GATGTATTTTCGCTTCAGAGAAAATTTATTAGACTTCCAGACAAATAACGATCAAGATTA
GTTGATGACTTTTTAATGAGTAAATATTGTCATTTAAGCCTGTCTATGTTCTAACATTTA
TATCGTATTTATTTTATAATTATTTTCTTAATCTCCATTTCAATGACTGTACAATATTCC
AAAAAACCTCATTTATTTGCTTTCATTTCTGTCATACATATACGCATAAAAATAAATGTG
AAAGGCTTTGAATTGTTGTTATGAATGCATTAAATACAATTTTTGAATAAATTTAATTTT
CTATGAAAATAATGAAAAGATTACAAATGAAGACATATTTAGATGAATGCGTAGATTTGC
ACGAAAACTGTAGTCTCTTACACGAAAAAACTCTACTTTCTGTCTAAAATTAATTTACAA
ATGTCCTACTTTTTTTCTTAAACAAGAAATTTGAAATAATAAAAAAGTTTCTGAAAAATA
TCTTTTTAAAAGTTATTTTATATATTTTGTTACAGATTACGATTTAAAAATGAAATCGTT
TTATTCATGGAAAAAAATTTTTATTCTCACAATTTGCTATGCATTACTATAATGAAAC
>g12745.t3 Gene=g12745 Length=464
MLTSTAKHILHCIALGVVITFFLFSSGAIKTSDDDYLAIDPWEEYGTFMTIFLYTIRSIS
ILILPQMLCNFLGLVCFNAFPDKVVLKGSPLLAPFICIRVVTRGDFADLVKANVMRNMNT
CLDTGLENFLIEVVTDKAVNLPKHRRIREIVVPKDYKTKSGALFKARALQYCLEDNVNVL
NPNDWIVHLDEETILTENSVRGITNFVLDGKHPFGQGLITYANESIVNIFTTLADSFRVS
DDMGKLRFQFKVFHKPPFSWKGSYVVTQLHAEKDVSFDNGMDGSVAEDCYFAMKAFSRGY
TFDFIDGEMYEKSPFTILDFIQQRKRWLQGILLVIHSKAIPFKYKSFLALSVYSWIMMPL
SSSNIFFAIYMPIPCPFILDLICSFLGGISVYMYVYGVYKSFPLKRIGFFKSVLYIIGAL
AMIPFNLVIENIAVMWGVLSNKHRFYVVQKEVSMTTEQRPLMTV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g12745.t3 | PANTHER | PTHR16779 | BETA-1,4-MANNOSYLTRANSFERASE EGH | 3 | 450 | 6.4E-147 |
| 1 | g12745.t3 | Pfam | PF13632 | Glycosyl transferase family group 2 | 185 | 372 | 6.6E-42 |
| 9 | g12745.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 11 | - |
| 19 | g12745.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 12 | 31 | - |
| 14 | g12745.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 32 | 50 | - |
| 18 | g12745.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 51 | 77 | - |
| 10 | g12745.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 78 | 346 | - |
| 17 | g12745.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 347 | 371 | - |
| 12 | g12745.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 372 | 376 | - |
| 16 | g12745.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 377 | 396 | - |
| 11 | g12745.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 397 | 407 | - |
| 15 | g12745.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 408 | 429 | - |
| 13 | g12745.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 430 | 464 | - |
| 8 | g12745.t3 | SUPERFAMILY | SSF53448 | Nucleotide-diphospho-sugar transferases | 165 | 378 | 2.11E-7 |
| 4 | g12745.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 29 | - |
| 3 | g12745.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 55 | 77 | - |
| 5 | g12745.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 351 | 373 | - |
| 6 | g12745.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 377 | 399 | - |
| 7 | g12745.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 412 | 434 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0019187 | beta-1,4-mannosyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K02359 (beta-1,4-mannosyltransferase [EC:2.4.1.-])
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
