General information for g12795.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Mannosyl-oligosaccharide alpha-1,2-mannosidase IA.
Parent gene
- The parent gene g12795 is located on chr_1: 26234202..26236399 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12795 | g12795.t1 | isoform | g12795.t1 | 26234202 | 26236399 |
| chr_1 | g12795 | g12795.t1 | exon | g12795.t1.exon1 | 26234202 | 26234534 |
| chr_1 | g12795 | g12795.t1 | cds | g12795.t1.CDS1 | 26234202 | 26234534 |
| chr_1 | g12795 | g12795.t1 | exon | g12795.t1.exon2 | 26234647 | 26234865 |
| chr_1 | g12795 | g12795.t1 | cds | g12795.t1.CDS2 | 26234647 | 26234865 |
| chr_1 | g12795 | g12795.t1 | exon | g12795.t1.exon3 | 26234961 | 26235354 |
| chr_1 | g12795 | g12795.t1 | cds | g12795.t1.CDS3 | 26234961 | 26235354 |
| chr_1 | g12795 | g12795.t1 | exon | g12795.t1.exon4 | 26235416 | 26235782 |
| chr_1 | g12795 | g12795.t1 | cds | g12795.t1.CDS4 | 26235416 | 26235782 |
| chr_1 | g12795 | g12795.t1 | exon | g12795.t1.exon5 | 26235860 | 26236004 |
| chr_1 | g12795 | g12795.t1 | cds | g12795.t1.CDS5 | 26235860 | 26236004 |
| chr_1 | g12795 | g12795.t1 | exon | g12795.t1.exon6 | 26236062 | 26236178 |
| chr_1 | g12795 | g12795.t1 | cds | g12795.t1.CDS6 | 26236062 | 26236178 |
| chr_1 | g12795 | g12795.t1 | exon | g12795.t1.exon7 | 26236265 | 26236399 |
| chr_1 | g12795 | g12795.t1 | cds | g12795.t1.CDS7 | 26236265 | 26236399 |
| chr_1 | g12795 | g12795.t1 | TTS | g12795.t1 | 26237170 | 26237170 |
| chr_1 | g12795 | g12795.t1 | TSS | g12795.t1 | NA | NA |
Sequences
>g12795.t1 Gene=g12795 Length=1710
ATGTCTTCAACATCTACACATCTTGTATGGTCAGCTGTGACCATTGTTGCATTAACTGGA
ATAACATTGACAACATTGAAATATAATGAAACTTCAAATGATATTAGTGATGATTATTTA
GACAATGATGAAGATTTTAATTTTAATCAAACTCAGGAATATTTTAACTATCATAATCCG
AAATTTCATTATGAAATAAAACAAAATGGGTCACTTATGATTACCGAAAGAAGGGTCGTT
AAACGTTCAAGTGACGATGATGTCATTCAATATTCAATTGATGATAAAAATTCATCAATG
ATTGACGAAGCGAAACGTCAAAAGGTCAAAGAGATGATGCAACATGCATGGAGCAATTAT
AAAACGTATGCATGGGGAAAGAATGAATTAAAACCTATTTCTAAGCGAGCTCATACAGGC
AGCATATTTGGTTCATATGATATTGGAGCGACAATTGTTGACTCAATGGATACACTTTAC
ATTATGGGAATGAAGGATGAATTTGATGAAGGCCGCGAGTGGATTGCCAAAAAGTTTACA
TTAGACAATGTTAGCGCCGACATTTCGGTATTTGAGACAAATATTCGTTTCGTAGGAGGA
TTTCTTGCTGCTTATGCACTCACAGGCGATCCAATCTTCAAAGAAAAAGCACAATATGTT
GCTGACAAGCTTTTGCCTGCATTCCAAACACCAACTGGAATTCCTTATGCTCTAATAAAC
TTCCGAACAGGCAGTAGCAAAAATTATGGATGGGCCAGTGGTGGCAGTAGTATTTTATCA
GAATTCGGAACATTACATTTAGAATTTGTTTATCTTTCGGACATCACAGGCAATCCTGTC
TATCGTGAGCGTGTCATGAACATTCGTTCAATACTCAAAGACTTGGAAAAGCCAAGGGGA
CTTTATCCAAATTATTTAAATCCAAAGACGGGTAAATGGGGACAACAACACATGTCTCTT
GGTGCACTCGGTGATAGCTTTTTTGAATATTTACTCAAAGCATGGCTACAATCGGGTCAG
ACTGATGAAGAAGCACGTGACATGTATGATGAAGCTATGGTAGCTATAATAGAGCACATG
GTGAGAAAGAGCAGTCCAAGTGGTCTTACATATGTGTCAGACATGAAATTTGATAGACTA
GAACACAAAATGGATCATCTTGCATGTTTTGCGGGTGGTCTATTTGGACTCGGTTCGCGT
ACACTTCAAAATCAACATGCTGACAAATATATGGAAGTAGCAGAAGGCATCACAAACACA
TGTCACGAGAGTTATATTCGAACATATACACATTTGGGACCAGAATCATTTAGATTTAGT
GATGCAGTCGAAGCCAAAGCACTCAAATCACAAGAGAAATATTATATTCTCCGTCCAGAG
ACGTTTGAGAGCTATTTCATAATGTGGCGGCTAACACACGATCAAAAATATCGTGATTGG
GGATGGGATGCTATACAAGCTCTCGAAAAGCATTGTCGATCAGATGGAGGCTATAGTGGA
ATAAAAAATGTCTATCTTGATCCACCACAACAAGATGATGTCCAACAGAGTTTTTTCCTT
GCTGAAAGCCTAAAGTACTTATATTTGTTATTCTCTGACGACTCCCTGCTACCGTTAGAT
GAGTGGGTATTTAACACGGAGGCGCATCCGTTGCCAATTAGGAATGCAAATCCACTTTAT
CGTTTAGCTGCACCATCAAGTTCGCCTTAA
>g12795.t1 Gene=g12795 Length=569
MSSTSTHLVWSAVTIVALTGITLTTLKYNETSNDISDDYLDNDEDFNFNQTQEYFNYHNP
KFHYEIKQNGSLMITERRVVKRSSDDDVIQYSIDDKNSSMIDEAKRQKVKEMMQHAWSNY
KTYAWGKNELKPISKRAHTGSIFGSYDIGATIVDSMDTLYIMGMKDEFDEGREWIAKKFT
LDNVSADISVFETNIRFVGGFLAAYALTGDPIFKEKAQYVADKLLPAFQTPTGIPYALIN
FRTGSSKNYGWASGGSSILSEFGTLHLEFVYLSDITGNPVYRERVMNIRSILKDLEKPRG
LYPNYLNPKTGKWGQQHMSLGALGDSFFEYLLKAWLQSGQTDEEARDMYDEAMVAIIEHM
VRKSSPSGLTYVSDMKFDRLEHKMDHLACFAGGLFGLGSRTLQNQHADKYMEVAEGITNT
CHESYIRTYTHLGPESFRFSDAVEAKALKSQEKYYILRPETFESYFIMWRLTHDQKYRDW
GWDAIQALEKHCRSDGGYSGIKNVYLDPPQQDDVQQSFFLAESLKYLYLLFSDDSLLPLD
EWVFNTEAHPLPIRNANPLYRLAAPSSSP
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g12795.t1 | Gene3D | G3DSA:1.50.10.10 | - | 89 | 560 | 1.0E-197 |
| 2 | g12795.t1 | PANTHER | PTHR11742:SF31 | MANNOSYL-OLIGOSACCHARIDE 1,2-ALPHA-MANNOSIDASE IA | 103 | 557 | 3.0E-215 |
| 3 | g12795.t1 | PANTHER | PTHR11742 | MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED | 103 | 557 | 3.0E-215 |
| 8 | g12795.t1 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 112 | 132 | 1.8E-84 |
| 6 | g12795.t1 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 149 | 163 | 1.8E-84 |
| 4 | g12795.t1 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 187 | 205 | 1.8E-84 |
| 10 | g12795.t1 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 226 | 245 | 1.8E-84 |
| 9 | g12795.t1 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 321 | 338 | 1.8E-84 |
| 7 | g12795.t1 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 383 | 399 | 1.8E-84 |
| 11 | g12795.t1 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 456 | 480 | 1.8E-84 |
| 5 | g12795.t1 | PRINTS | PR00747 | Glycosyl hydrolase family 47 signature | 517 | 537 | 1.8E-84 |
| 1 | g12795.t1 | Pfam | PF01532 | Glycosyl hydrolase family 47 | 112 | 553 | 7.2E-156 |
| 15 | g12795.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 6 | - |
| 17 | g12795.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 26 | - |
| 16 | g12795.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 27 | 569 | - |
| 13 | g12795.t1 | SUPERFAMILY | SSF48225 | Seven-hairpin glycosidases | 100 | 553 | 8.24E-175 |
| 12 | g12795.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 26 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005509 | calcium ion binding | MF |
| GO:0004571 | mannosyl-oligosaccharide 1,2-alpha-mannosidase activity | MF |
| GO:0005975 | carbohydrate metabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K01230 (mannosyl-oligosaccharide alpha-1,2-mannosidase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
