General information for g12821.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Dolichyl-diphosphooligosaccharide–protein glycosyltransferase subunit STT3A.
Parent gene
- The parent gene g12821 is located on chr_1: 26432183..26433574 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12821 | g12821.t3 | TTS | g12821.t3 | 26428786 | 26428786 |
| chr_1 | g12821 | g12821.t3 | isoform | g12821.t3 | 26429064 | 26431378 |
| chr_1 | g12821 | g12821.t3 | exon | g12821.t3.exon1 | 26429064 | 26429619 |
| chr_1 | g12821 | g12821.t3 | cds | g12821.t3.CDS1 | 26429618 | 26429619 |
| chr_1 | g12821 | g12821.t3 | exon | g12821.t3.exon2 | 26430067 | 26430193 |
| chr_1 | g12821 | g12821.t3 | cds | g12821.t3.CDS2 | 26430067 | 26430193 |
| chr_1 | g12821 | g12821.t3 | exon | g12821.t3.exon3 | 26430248 | 26430650 |
| chr_1 | g12821 | g12821.t3 | cds | g12821.t3.CDS3 | 26430248 | 26430650 |
| chr_1 | g12821 | g12821.t3 | exon | g12821.t3.exon4 | 26430709 | 26430811 |
| chr_1 | g12821 | g12821.t3 | cds | g12821.t3.CDS4 | 26430709 | 26430811 |
| chr_1 | g12821 | g12821.t3 | exon | g12821.t3.exon5 | 26430866 | 26431378 |
| chr_1 | g12821 | g12821.t3 | cds | g12821.t3.CDS5 | 26430866 | 26431307 |
| chr_1 | g12821 | g12821.t3 | TSS | g12821.t3 | NA | NA |
Sequences
>g12821.t3 Gene=g12821 Length=1702
CAATTGTTCCTGGTTACATTTCAAGATCAGTAGCTGGTTCATATGATAACGAAGGAATTG
CAATTTTTGCTATGCTTCTTACTTATTATTTATGGATTAAAGCTGTTAAAACAGGCACAA
TTTGTTGGTCTGCTCTTGCTAGTTTGGCATACTTTTATATGGTTTCTAGTTGGGGTGGTT
ACGTGTTTTTAATCAACTTGATTCCTTTGCACGTGCTAGCTTTAATGATCACAGGAAGAT
TTTCACATCGTGTATATGTGGCATATAGCTCTGTTTATACAATCGGAACAATTTTGAGCA
TGCAAATCTCATTCGTCGGATTTCAACCAGTTCAATCATCAGAACATATGCTTGCATTTG
GAACATTTGGATTGTGCCAACTTCATGCACTTGTTGATTTCCTCAAGTCAAAATTATCAA
AAGAACAATTTGAAGTATTATTCAAATCAATTGCTATAGCTATTCTTTCAATTTTCCTTG
GTTTTGCTGCAGTTCTTACTTTATCAGGAAAAATTTCACCTTGGACTGGACGTTTCTACT
CTCTATTGGACCCTTCATATGCCAAAAATCATATTCCTATCATTGCTTCTGTATCTGAGC
ATCAGCCTACAAGTTGGTCATCATTCTATTTTGATCTTCAAATATTAGTTTTCTTATTCC
CTGCTGGACTTTACTTCTGTTTTGATAAACTCACTGATGCCAACATCTTTATTATTCTCT
ATGGAGTTACCTCAATTTACTTTACAGGAGTTATGGTCCGATTAATGCTAGTTCTTGCAC
CAGTTATGTGCATTCTCTCTGGAATTGCTGTTTCTCATTTGCTTTCAAAATACATAAGAA
ATGTGGATGCCTCAGTTAGCGTATTGAGTAGCGATGGAAAGTCACAGCCACGTCAAGAAT
CTTCCAAAAAGTACAAGAAATATGATCAGCAACAAAAAGGAATTAAAAGTGAAATTGCTA
TTGGATTTGTTGTATTGACAACATTTATGTTGATAAGCTTTGTTTTCCATTCAGTTTGGG
TTACTTCTGAGGCGTATAGTTCACCATCAATTGTGTTATCAGCAAGATCTCACGATGGCA
GTAGAATAATTTTTGATGATTTCCGTGAAGCATATTATTGGCTAAGAATGAATACAGCTG
AGGTATAAAATGAATATTGTGCAACAGGTGGGATTATGGTTACCAAATAACTGCGATGGC
AAATAGAACAGTGCTTGTGGATAATAATACTTGGAACAATACACATATTTCTCGAGTTGG
ACAAGCTATGGCATCAAGTGAAGAAAAAGCGTATGAAATTTTACGTGAACTGGATGTCAA
TTATGTTTTGGTAATTTTTGGTGGCTTGACTGGTTATTCATCTGATGATATCAATAAATT
CTTGTGGATGGTGAGAATTGGTGGTTCAACTGATCGTGGAAGTCATATTAAAGAACAAGA
TTATTATTCGTCGAATGGTGACTTTAGAGTTGATAAAGAAGGTTCTCAAACTTTATTGAA
TTGTTTAATGTACAAACTCTGTTATTATCGCTTTGGTAATGTCTATACTGAAGCAGGAAA
ACCAACAGGTTATGATAGAGTTAGAGGAGCGGAAATTGGTAACAAAGATTTTGAACTAGA
AACACTTGAAGAAGTATACACAACTGAGCATTGGCTTGTGAGAATCTATAAAGTGAAAGA
TTTAGAAAATCGCGGCGTATAA
>g12821.t3 Gene=g12821 Length=358
MLLTYYLWIKAVKTGTICWSALASLAYFYMVSSWGGYVFLINLIPLHVLALMITGRFSHR
VYVAYSSVYTIGTILSMQISFVGFQPVQSSEHMLAFGTFGLCQLHALVDFLKSKLSKEQF
EVLFKSIAIAILSIFLGFAAVLTLSGKISPWTGRFYSLLDPSYAKNHIPIIASVSEHQPT
SWSSFYFDLQILVFLFPAGLYFCFDKLTDANIFIILYGVTSIYFTGVMVRLMLVLAPVMC
ILSGIAVSHLLSKYIRNVDASVSVLSSDGKSQPRQESSKKYKKYDQQQKGIKSEIAIGFV
VLTTFMLISFVFHSVWVTSEAYSSPSIVLSARSHDGSRIIFDDFREAYYWLRMNTAEV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g12821.t3 | PANTHER | PTHR13872:SF43 | DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE–PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3A | 1 | 357 | 5.7E-181 |
| 3 | g12821.t3 | PANTHER | PTHR13872 | DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE–PROTEIN GLYCOSYLTRANSFERASE SUBUNIT | 1 | 357 | 5.7E-181 |
| 1 | g12821.t3 | Pfam | PF02516 | Oligosaccharyl transferase STT3 subunit | 1 | 323 | 9.1E-79 |
| 14 | g12821.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 6 | - |
| 25 | g12821.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 28 | - |
| 21 | g12821.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 29 | 33 | - |
| 24 | g12821.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 34 | 54 | - |
| 16 | g12821.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 55 | 60 | - |
| 30 | g12821.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 61 | 81 | - |
| 19 | g12821.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 82 | 92 | - |
| 23 | g12821.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 93 | 111 | - |
| 13 | g12821.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 112 | 122 | - |
| 31 | g12821.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 123 | 144 | - |
| 22 | g12821.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 145 | 184 | - |
| 27 | g12821.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 185 | 203 | - |
| 15 | g12821.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 204 | 209 | - |
| 28 | g12821.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 210 | 226 | - |
| 20 | g12821.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 227 | 231 | - |
| 29 | g12821.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 232 | 251 | - |
| 17 | g12821.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 252 | 294 | - |
| 26 | g12821.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 295 | 316 | - |
| 18 | g12821.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 317 | 358 | - |
| 9 | g12821.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 29 | - |
| 8 | g12821.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 33 | 55 | - |
| 7 | g12821.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 62 | 84 | - |
| 4 | g12821.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 94 | 111 | - |
| 6 | g12821.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 123 | 145 | - |
| 12 | g12821.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 182 | 204 | - |
| 5 | g12821.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 211 | 228 | - |
| 10 | g12821.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 232 | 251 | - |
| 11 | g12821.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 295 | 317 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0006486 | protein glycosylation | BP |
| GO:0004576 | oligosaccharyl transferase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
