General information for g12834.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Multifunctional protein ADE2.
Parent gene
- The parent gene g12834 is located on chr_1: 26473015..26477102 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12834 | g12834.t2 | isoform | g12834.t2 | 26471416 | 26473015 |
| chr_1 | g12834 | g12834.t2 | exon | g12834.t2.exon1 | 26471416 | 26471530 |
| chr_1 | g12834 | g12834.t2 | TSS | g12834.t2 | 26471425 | 26471425 |
| chr_1 | g12834 | g12834.t2 | exon | g12834.t2.exon2 | 26471751 | 26473015 |
| chr_1 | g12834 | g12834.t2 | cds | g12834.t2.CDS1 | 26472017 | 26473015 |
| chr_1 | g12834 | g12834.t2 | TTS | g12834.t2 | 26473111 | 26473111 |
Sequences
>g12834.t2 Gene=g12834 Length=1380
AGTCGGCTAAAATCAGTCAAGCATTGACATTCATTTTAAATATAGTATTTGCTCTGGAAA
CATCAATCTATTTTTGAAATCTCGTTAGAGTGAATATAAATTATAAATATCAAACTCGGT
GATTACAAACTCGGAAAACTTTTGAATGAAGGAAAGACTAAACAAATTTATGATCTTCCT
AATAATCCGGGCTATGTCTTGCTATTAAATAAAGATAGAATCAGTGCTCATAATGGTGTC
CGCGTTCACGATCTTGAGGGAAAAGCTGAAATTTCTAATAAGACAAATGCAAAAGTTTTT
GAGCTTTTGAATATTGCTGGAGTCAAAACAGCTTTTGTTAAATTGGCTTCTGATAATGCA
TTTATTGCTCGCAAATGTGAGATGGTGCCGATTGAATGGGTCACAAGACGTTTGGCTACT
GGTTCATTTTTGAAGAGAAATCCAGGAATTCAAGAAGGACTTCGCTTCTGTCCACCAAAA
CAGGAAACATTTTTCAAAGATGATGCAAACGATGATCCACAATGGAGTGAAGAACAGATT
GTAGCAGCAAAGTTCAAAGTCAATAACTTATTAATCACACAAGATGAAGTTGATATTATG
AGACAAACAACAATTCTCGTCTTTGAAATCCTCGAACGTGCATGGACAGAGAAAAACTGT
GCACTCATTGATATGAAAATTGAATTTGGAGTAGATGAAAACGGCGAAATACTTGTTGCT
GATGTGATAGACAGCGATTCATGGCGTTTGTGGCCTTCGGGTGATAAACGTTTGATGGTC
GATAAGCAAGTATACAGAAATTTAACTACAGTTACAAGTAAAGATTTGGAAACTGTCAAA
AGAAATTTCCAATGGGTTCATGATCAATTGTCTGAAATTTTACCTAAAAATGATCATTTG
GTTGTGATTATTATGGGTTCACCTTCTGATAAGGAACACTGTCAAAAAATTGCCAAACAT
TGTAATGAACTTGGACTTAATACAGAAATGCGCGTTTCTTCAGCACACAAAGTTACAAAG
GGCACACTTCAAATCATGTCAGAATATGAAGGAATTATCAAAAATCTCGTCTTTATCACA
GTTGCTGGTCGTTCAAACGGACTCGGACCAGTTTTATCTGGCAACACATCTTATCCAGTG
ATTAACTGTCCACCAATCAAAAGTGATACCATGAACATTGACTTGTGGTCTTCAATCAAC
TTGCCATCCGGTTTAGGATGTAGCACAGTAATCTATCCAGAAGCCGCAGCACTCAATGCA
GCTCAAATTTTGGGTCTCAGCAACTTCATGATTTGGTCACGACTTAGAGTCAAGATGATT
AATAATCTGATAAGCTTGAAAAAAGCTGATAAAGAATTGCGTGGAGTCAAGGAAGCTTAA
>g12834.t2 Gene=g12834 Length=332
MVPIEWVTRRLATGSFLKRNPGIQEGLRFCPPKQETFFKDDANDDPQWSEEQIVAAKFKV
NNLLITQDEVDIMRQTTILVFEILERAWTEKNCALIDMKIEFGVDENGEILVADVIDSDS
WRLWPSGDKRLMVDKQVYRNLTTVTSKDLETVKRNFQWVHDQLSEILPKNDHLVVIIMGS
PSDKEHCQKIAKHCNELGLNTEMRVSSAHKVTKGTLQIMSEYEGIIKNLVFITVAGRSNG
LGPVLSGNTSYPVINCPPIKSDTMNIDLWSSINLPSGLGCSTVIYPEAAALNAAQILGLS
NFMIWSRLRVKMINNLISLKKADKELRGVKEA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g12834.t2 | Gene3D | G3DSA:3.30.470.20 | - | 1 | 143 | 3.4E-54 |
| 12 | g12834.t2 | Gene3D | G3DSA:3.40.50.7700 | - | 171 | 300 | 7.7E-48 |
| 5 | g12834.t2 | Hamap | MF_02045 | Phosphoribosylaminoimidazole carboxylase [purE]. | 172 | 327 | 34.577671 |
| 3 | g12834.t2 | PANTHER | PTHR43599:SF3 | MULTIFUNCTIONAL PROTEIN ADE2 | 1 | 329 | 7.4E-157 |
| 4 | g12834.t2 | PANTHER | PTHR43599 | MULTIFUNCTIONAL PROTEIN ADE2 | 1 | 329 | 7.4E-157 |
| 1 | g12834.t2 | Pfam | PF01259 | SAICAR synthetase | 1 | 147 | 2.2E-33 |
| 2 | g12834.t2 | Pfam | PF00731 | AIR carboxylase | 174 | 310 | 3.9E-35 |
| 10 | g12834.t2 | ProSitePatterns | PS01057 | SAICAR synthetase signature 1. | 1 | 15 | - |
| 9 | g12834.t2 | ProSitePatterns | PS01058 | SAICAR synthetase signature 2. | 95 | 103 | - |
| 8 | g12834.t2 | SMART | SM01001 | AIRC_2 | 172 | 319 | 7.9E-28 |
| 6 | g12834.t2 | SUPERFAMILY | SSF56104 | SAICAR synthase-like | 1 | 159 | 1.54E-42 |
| 7 | g12834.t2 | SUPERFAMILY | SSF52255 | N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE) | 173 | 328 | 7.06E-37 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004639 | phosphoribosylaminoimidazolesuccinocarboxamide synthase activity | MF |
| GO:0006164 | purine nucleotide biosynthetic process | BP |
| GO:0006189 | ‘de novo’ IMP biosynthetic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
