General information for g12834.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Multifunctional protein ADE2.
Parent gene
- The parent gene g12834 is located on chr_1: 26473015..26477102 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12834 | g12834.t5 | TSS | g12834.t5 | 26471425 | 26471425 |
| chr_1 | g12834 | g12834.t5 | isoform | g12834.t5 | 26471536 | 26473015 |
| chr_1 | g12834 | g12834.t5 | exon | g12834.t5.exon1 | 26471536 | 26472395 |
| chr_1 | g12834 | g12834.t5 | cds | g12834.t5.CDS1 | 26472017 | 26472395 |
| chr_1 | g12834 | g12834.t5 | exon | g12834.t5.exon2 | 26472447 | 26473015 |
| chr_1 | g12834 | g12834.t5 | cds | g12834.t5.CDS2 | 26472447 | 26473015 |
| chr_1 | g12834 | g12834.t5 | TTS | g12834.t5 | 26473111 | 26473111 |
Sequences
>g12834.t5 Gene=g12834 Length=1429
ATGGCAGATATTAAACAAAGTAAGTTAAATTAAAATAAATTGACATCGGTTTCAACTGGT
AATTTGAGTGAGACATCGCGAAATTCAATTTAGAATATAAAAATGTGATCACTGAACTTA
TTTTCTTGATTCTTACTGGAATTTATTCTAGAATATTTGATGAAAACAACAGCCGGTTCA
ATAAATAAATTATTCTTATCATTATCTCTTTTCAGTCGGTGATTACAAACTCGGAAAACT
TTTGAATGAAGGAAAGACTAAACAAATTTATGATCTTCCTAATAATCCGGGCTATGTCTT
GCTATTAAATAAAGATAGAATCAGTGCTCATAATGGTGTCCGCGTTCACGATCTTGAGGG
AAAAGCTGAAATTTCTAATAAGACAAATGCAAAAGTTTTTGAGCTTTTGAATATTGCTGG
AGTCAAAACAGCTTTTGTTAAATTGGCTTCTGATAATGCATTTATTGCTCGCAAATGTGA
GATGGTGCCGATTGAATGGGTCACAAGACGTTTGGCTACTGGTTCATTTTTGAAGAGAAA
TCCAGGAATTCAAGAAGGACTTCGCTTCTGTCCACCAAAACAGGAAACATTTTTCAAAGA
TGATGCAAACGATGATCCACAATGGAGTGAAGAACAGATTGTAGCAGCAAAGTTCAAAGT
CAATAACTTATTAATCACACAAGATGAAGTTGATATTATGAGACAAACAACAATTCTCGT
CTTTGAAATCCTCGAACGTGCATGGACAGAGAAAAACTGTGCACTCATTGATATGAAAAT
TGAATTTGGAGTAGATGAAAACGGCGAAATACTTGTTGCTGATGTGATAGACAGCGATTC
ATGGCGTTTGTGGCCTTCGGTTACAAGTAAAGATTTGGAAACTGTCAAAAGAAATTTCCA
ATGGGTTCATGATCAATTGTCTGAAATTTTACCTAAAAATGATCATTTGGTTGTGATTAT
TATGGGTTCACCTTCTGATAAGGAACACTGTCAAAAAATTGCCAAACATTGTAATGAACT
TGGACTTAATACAGAAATGCGCGTTTCTTCAGCACACAAAGTTACAAAGGGCACACTTCA
AATCATGTCAGAATATGAAGGAATTATCAAAAATCTCGTCTTTATCACAGTTGCTGGTCG
TTCAAACGGACTCGGACCAGTTTTATCTGGCAACACATCTTATCCAGTGATTAACTGTCC
ACCAATCAAAAGTGATACCATGAACATTGACTTGTGGTCTTCAATCAACTTGCCATCCGG
TTTAGGATGTAGCACAGTAATCTATCCAGAAGCCGCAGCACTCAATGCAGCTCAAATTTT
GGGTCTCAGCAACTTCATGATTTGGTCACGACTTAGAGTCAAGATGATTAATAATCTGAT
AAGCTTGAAAAAAGCTGATAAAGAATTGCGTGGAGTCAAGGAAGCTTAA
>g12834.t5 Gene=g12834 Length=315
MVPIEWVTRRLATGSFLKRNPGIQEGLRFCPPKQETFFKDDANDDPQWSEEQIVAAKFKV
NNLLITQDEVDIMRQTTILVFEILERAWTEKNCALIDMKIEFGVDENGEILVADVIDSDS
WRLWPSVTSKDLETVKRNFQWVHDQLSEILPKNDHLVVIIMGSPSDKEHCQKIAKHCNEL
GLNTEMRVSSAHKVTKGTLQIMSEYEGIIKNLVFITVAGRSNGLGPVLSGNTSYPVINCP
PIKSDTMNIDLWSSINLPSGLGCSTVIYPEAAALNAAQILGLSNFMIWSRLRVKMINNLI
SLKKADKELRGVKEA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g12834.t5 | Gene3D | G3DSA:3.30.470.20 | - | 1 | 141 | 4.0E-51 |
| 12 | g12834.t5 | Gene3D | G3DSA:3.40.50.7700 | - | 154 | 283 | 6.9E-48 |
| 5 | g12834.t5 | Hamap | MF_02045 | Phosphoribosylaminoimidazole carboxylase [purE]. | 155 | 310 | 34.577671 |
| 3 | g12834.t5 | PANTHER | PTHR43599:SF3 | MULTIFUNCTIONAL PROTEIN ADE2 | 1 | 312 | 7.3E-143 |
| 4 | g12834.t5 | PANTHER | PTHR43599 | MULTIFUNCTIONAL PROTEIN ADE2 | 1 | 312 | 7.3E-143 |
| 1 | g12834.t5 | Pfam | PF01259 | SAICAR synthetase | 1 | 133 | 6.5E-30 |
| 2 | g12834.t5 | Pfam | PF00731 | AIR carboxylase | 157 | 293 | 3.5E-35 |
| 10 | g12834.t5 | ProSitePatterns | PS01057 | SAICAR synthetase signature 1. | 1 | 15 | - |
| 9 | g12834.t5 | ProSitePatterns | PS01058 | SAICAR synthetase signature 2. | 95 | 103 | - |
| 8 | g12834.t5 | SMART | SM01001 | AIRC_2 | 155 | 302 | 7.9E-28 |
| 6 | g12834.t5 | SUPERFAMILY | SSF56104 | SAICAR synthase-like | 1 | 155 | 3.6E-39 |
| 7 | g12834.t5 | SUPERFAMILY | SSF52255 | N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE) | 156 | 311 | 6.28E-37 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004639 | phosphoribosylaminoimidazolesuccinocarboxamide synthase activity | MF |
| GO:0006164 | purine nucleotide biosynthetic process | BP |
| GO:0006189 | ‘de novo’ IMP biosynthetic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed