General information for g12864.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable cytochrome P450 6a13.
Parent gene
- The parent gene g12864 is located on chr_1: 26631566..26633456 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12864 | g12864.t1 | TTS | g12864.t1 | 26631534 | 26631534 |
| chr_1 | g12864 | g12864.t1 | isoform | g12864.t1 | 26631566 | 26633456 |
| chr_1 | g12864 | g12864.t1 | exon | g12864.t1.exon1 | 26631566 | 26631733 |
| chr_1 | g12864 | g12864.t1 | cds | g12864.t1.CDS1 | 26631566 | 26631733 |
| chr_1 | g12864 | g12864.t1 | exon | g12864.t1.exon2 | 26631793 | 26632517 |
| chr_1 | g12864 | g12864.t1 | cds | g12864.t1.CDS2 | 26631793 | 26632517 |
| chr_1 | g12864 | g12864.t1 | exon | g12864.t1.exon3 | 26632817 | 26633049 |
| chr_1 | g12864 | g12864.t1 | cds | g12864.t1.CDS3 | 26632817 | 26633049 |
| chr_1 | g12864 | g12864.t1 | exon | g12864.t1.exon4 | 26633107 | 26633456 |
| chr_1 | g12864 | g12864.t1 | cds | g12864.t1.CDS4 | 26633107 | 26633456 |
| chr_1 | g12864 | g12864.t1 | TSS | g12864.t1 | 26633479 | 26633479 |
Sequences
>g12864.t1 Gene=g12864 Length=1476
ATGAGCACAACTTTAGTAGCATTGATCATCATTTTATTTAGTGTTGCTTACATTTTTGTA
AAGCGACAATTTAGTTATTGGTCACGAAGAGGATTTTTTCAAGCTGACGCTTCGTTTCCT
TTTGGATCAATTAAAGGAGCAAATTTTTCTATAACATTGACTGAAAAATTGGATGAAATT
TATAAACAATTCAAAGGAAAAGCTTCTGTCGTAGGTTTTTATAATTTCTTAGGAGCATCA
ATTTTACCTATAGATCCAGAAATTATAAAACACATTTTTGTCAAAGATTTTGCTTCATTT
CATGATCGAGGATTTTTTTATAATAAAAGAGATGATCCGCTTACTAATAACATTCTTTCT
ATCAAAGGTCAAGAATGGAAAGATCGTAGGGCTAAATTCACTCCTGTATTTACTTCAGCT
AAAATGAAGCAAATGTATGAAATTATTGAAGAAATTAGTGACAAATTTGCTCTTAAACTC
GGTGAAAGTTTAAAAGACTCAAATATTCAAGACATGAGAATTTGGGCACGAAGATTTACA
AATGATAATATTGGTAGTACTGTGTTTGGAATTGACGCAAAATGTCTTGAAGATATAAAT
TCTGATTTGATGGTGTTTGGTCAAAAATCCCTTAACATGACACTTGCGGGTGCATTTAAA
TTTGCATTTGGTTCAACTTTTCCTGAAATTGCAAGATTTTTACGATTTAAAATTCTTGAC
AAAGATTCACATGAGTTTTTCCTAAATACATTTCTTCAAACTTTAGAATACCGAAGAAAT
AATAAGCAAATTGTTCGTCATGATTTTGTGTCAATGCTGTTAGAATTATCGGACCAATTA
CCACCAATTGATCTTGCTGCTGAAGCCACAATAATGTATTTAGCTGGTTTTGAAACAAGT
GCAACTTTGATTTCTTTTGCTTTGTATGAATTGGCTTTAAACTCAGAAATGCAAGATCGA
TTGAGAGATGAAATTTTAAATGGAATAAATGAAAATGATGGAAAAATTACTTATGAAATG
CTTTATGGATTTGAATATCTTGACATGGTTATAAACGAAACTTTAAGAAAACTTCCATCA
ATTCCACACATTTTTAGAAAATGCACAAAAGATTTTGTAGTGCCAAATACGAATCTTGTT
ATTCCAAAAGGAACTCAAGTTATTATTAATAATTACTCACTACAACATGATCCAGAATAT
TTTCCAAATCCTGAAAAATTTGATCCAGAAAGATTCAACAGTGAAAATAAAAAGAAAATT
AATCCGTATACAAATATGCCATTTGCACTTGGTAATCGTAATTGTTTGGGCGAGCGCTTT
GGATTAATGCAAGCTAAAATTGGCGTAGTAAAAATGATTAAAAATTTTATTTTTACACCA
TGTAATAAAACTACTATTCCAATGAAATTTGTTCCTAATCAATTTTTCTTAACTCCAGTT
GGTGGAATTCAATTATGCATCAAAAAAATTCATTAA
>g12864.t1 Gene=g12864 Length=491
MSTTLVALIIILFSVAYIFVKRQFSYWSRRGFFQADASFPFGSIKGANFSITLTEKLDEI
YKQFKGKASVVGFYNFLGASILPIDPEIIKHIFVKDFASFHDRGFFYNKRDDPLTNNILS
IKGQEWKDRRAKFTPVFTSAKMKQMYEIIEEISDKFALKLGESLKDSNIQDMRIWARRFT
NDNIGSTVFGIDAKCLEDINSDLMVFGQKSLNMTLAGAFKFAFGSTFPEIARFLRFKILD
KDSHEFFLNTFLQTLEYRRNNKQIVRHDFVSMLLELSDQLPPIDLAAEATIMYLAGFETS
ATLISFALYELALNSEMQDRLRDEILNGINENDGKITYEMLYGFEYLDMVINETLRKLPS
IPHIFRKCTKDFVVPNTNLVIPKGTQVIINNYSLQHDPEYFPNPEKFDPERFNSENKKKI
NPYTNMPFALGNRNCLGERFGLMQAKIGVVKMIKNFIFTPCNKTTIPMKFVPNQFFLTPV
GGIQLCIKKIH
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g12864.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 15 | 491 | 4.4E-122 |
| 2 | g12864.t1 | PANTHER | PTHR24292 | CYTOCHROME P450 | 7 | 487 | 1.4E-151 |
| 5 | g12864.t1 | PRINTS | PR00463 | E-class P450 group I signature | 284 | 301 | 5.7E-20 |
| 12 | g12864.t1 | PRINTS | PR00385 | P450 superfamily signature | 295 | 312 | 2.8E-11 |
| 7 | g12864.t1 | PRINTS | PR00463 | E-class P450 group I signature | 304 | 330 | 5.7E-20 |
| 4 | g12864.t1 | PRINTS | PR00463 | E-class P450 group I signature | 348 | 366 | 5.7E-20 |
| 11 | g12864.t1 | PRINTS | PR00385 | P450 superfamily signature | 349 | 360 | 2.8E-11 |
| 6 | g12864.t1 | PRINTS | PR00463 | E-class P450 group I signature | 390 | 414 | 5.7E-20 |
| 3 | g12864.t1 | PRINTS | PR00463 | E-class P450 group I signature | 425 | 435 | 5.7E-20 |
| 10 | g12864.t1 | PRINTS | PR00385 | P450 superfamily signature | 426 | 435 | 2.8E-11 |
| 8 | g12864.t1 | PRINTS | PR00463 | E-class P450 group I signature | 435 | 458 | 5.7E-20 |
| 9 | g12864.t1 | PRINTS | PR00385 | P450 superfamily signature | 435 | 446 | 2.8E-11 |
| 1 | g12864.t1 | Pfam | PF00067 | Cytochrome P450 | 49 | 486 | 4.0E-88 |
| 17 | g12864.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 18 | g12864.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 19 | g12864.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 12 | - |
| 20 | g12864.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 16 | - |
| 16 | g12864.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 491 | - |
| 14 | g12864.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 55 | 488 | 2.88E-106 |
| 13 | g12864.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 5 | 27 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K05917 (sterol 14alpha-demethylase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed