General information for g12864.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 6A1.
Parent gene
- The parent gene g12864 is located on chr_1: 26631566..26633456 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12864 | g12864.t3 | TTS | g12864.t3 | 26631534 | 26631534 |
| chr_1 | g12864 | g12864.t3 | isoform | g12864.t3 | 26631566 | 26633456 |
| chr_1 | g12864 | g12864.t3 | exon | g12864.t3.exon1 | 26631566 | 26631566 |
| chr_1 | g12864 | g12864.t3 | cds | g12864.t3.CDS1 | 26631566 | 26631566 |
| chr_1 | g12864 | g12864.t3 | exon | g12864.t3.exon2 | 26632196 | 26632517 |
| chr_1 | g12864 | g12864.t3 | cds | g12864.t3.CDS2 | 26632196 | 26632517 |
| chr_1 | g12864 | g12864.t3 | exon | g12864.t3.exon3 | 26632817 | 26633049 |
| chr_1 | g12864 | g12864.t3 | cds | g12864.t3.CDS3 | 26632817 | 26633049 |
| chr_1 | g12864 | g12864.t3 | exon | g12864.t3.exon4 | 26633107 | 26633456 |
| chr_1 | g12864 | g12864.t3 | cds | g12864.t3.CDS4 | 26633107 | 26633456 |
| chr_1 | g12864 | g12864.t3 | TSS | g12864.t3 | 26633479 | 26633479 |
Sequences
>g12864.t3 Gene=g12864 Length=906
ATGAGCACAACTTTAGTAGCATTGATCATCATTTTATTTAGTGTTGCTTACATTTTTGTA
AAGCGACAATTTAGTTATTGGTCACGAAGAGGATTTTTTCAAGCTGACGCTTCGTTTCCT
TTTGGATCAATTAAAGGAGCAAATTTTTCTATAACATTGACTGAAAAATTGGATGAAATT
TATAAACAATTCAAAGGAAAAGCTTCTGTCGTAGGTTTTTATAATTTCTTAGGAGCATCA
ATTTTACCTATAGATCCAGAAATTATAAAACACATTTTTGTCAAAGATTTTGCTTCATTT
CATGATCGAGGATTTTTTTATAATAAAAGAGATGATCCGCTTACTAATAACATTCTTTCT
ATCAAAGGTCAAGAATGGAAAGATCGTAGGGCTAAATTCACTCCTGTATTTACTTCAGCT
AAAATGAAGCAAATGTATGAAATTATTGAAGAAATTAGTGACAAATTTGCTCTTAAACTC
GGTGAAAGTTTAAAAGACTCAAATATTCAAGACATGAGAATTTGGGCACGAAGATTTACA
AATGATAATATTGGTAGTACTGTGTTTGGAATTGACGCAAAATGTCTTGAAGATATAAAT
TCTGATTTGATGGTGTTTGGTCAAAAATCCCTTAACATGACACTTGCGGGTGCATTTAAA
TTTGCATTTGGTTCAACTTTTCCTGAAATTGCAAGATTTTTACGATTTAAAATTCTTGAC
AAAGATTCACATGAGTTTTTCCTAAATACATTTCTTCAAACTTTAGAATACCGAAGAAAT
AATAAGCAAATTGTTCGTCATGATTTTGTGTCAATGCTGTTAGAATTATCGGACCAATTA
CCACCAATTGATCTTGCTGCTGAAGCCACAATAATGTATTTAGCTGGTTTTGAAACAAGT
GCAACA
>g12864.t3 Gene=g12864 Length=302
MSTTLVALIIILFSVAYIFVKRQFSYWSRRGFFQADASFPFGSIKGANFSITLTEKLDEI
YKQFKGKASVVGFYNFLGASILPIDPEIIKHIFVKDFASFHDRGFFYNKRDDPLTNNILS
IKGQEWKDRRAKFTPVFTSAKMKQMYEIIEEISDKFALKLGESLKDSNIQDMRIWARRFT
NDNIGSTVFGIDAKCLEDINSDLMVFGQKSLNMTLAGAFKFAFGSTFPEIARFLRFKILD
KDSHEFFLNTFLQTLEYRRNNKQIVRHDFVSMLLELSDQLPPIDLAAEATIMYLAGFETS
AT
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g12864.t3 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 15 | 302 | 3.9E-49 |
| 2 | g12864.t3 | PANTHER | PTHR24292 | CYTOCHROME P450 | 4 | 302 | 1.5E-72 |
| 3 | g12864.t3 | PANTHER | PTHR24292:SF95 | CYP6A16, ISOFORM B-RELATED | 4 | 302 | 1.5E-72 |
| 5 | g12864.t3 | PRINTS | PR00464 | Group II E-class P450 signature | 123 | 143 | 4.3E-6 |
| 4 | g12864.t3 | PRINTS | PR00464 | Group II E-class P450 signature | 178 | 196 | 4.3E-6 |
| 1 | g12864.t3 | Pfam | PF00067 | Cytochrome P450 | 49 | 302 | 3.2E-22 |
| 10 | g12864.t3 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 11 | g12864.t3 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 12 | g12864.t3 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 12 | - |
| 13 | g12864.t3 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 16 | - |
| 9 | g12864.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 302 | - |
| 7 | g12864.t3 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 54 | 302 | 1.28E-37 |
| 6 | g12864.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 5 | 27 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0004497 | monooxygenase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed