General information for g12935.t21
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial.
Parent gene
- The parent gene g12935 is located on chr_1: 27004491..27006329 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g12935 | g12935.t21 | TTS | g12935.t21 | 27004441 | 27004441 |
| chr_1 | g12935 | g12935.t21 | isoform | g12935.t21 | 27004491 | 27006329 |
| chr_1 | g12935 | g12935.t21 | exon | g12935.t21.exon1 | 27004491 | 27005593 |
| chr_1 | g12935 | g12935.t21 | cds | g12935.t21.CDS1 | 27004491 | 27005593 |
| chr_1 | g12935 | g12935.t21 | exon | g12935.t21.exon2 | 27005687 | 27005780 |
| chr_1 | g12935 | g12935.t21 | cds | g12935.t21.CDS2 | 27005687 | 27005780 |
| chr_1 | g12935 | g12935.t21 | exon | g12935.t21.exon3 | 27005842 | 27005901 |
| chr_1 | g12935 | g12935.t21 | cds | g12935.t21.CDS3 | 27005842 | 27005877 |
| chr_1 | g12935 | g12935.t21 | exon | g12935.t21.exon4 | 27006169 | 27006329 |
| chr_1 | g12935 | g12935.t21 | TSS | g12935.t21 | NA | NA |
Sequences
>g12935.t21 Gene=g12935 Length=1418
ACTACAAACATTTTTATTGATAAAAATTATTTTTTTTTTTGATAAAACTCTATCTTCATA
TATTGAATTCCTTTTAAGTAATTTCAAAAATTCCATTCAAACTGTCAAAATAATTATGAT
CACAAATCATATTTAAAAATGTGCTAATTAAATAAAATTTAAATAAAATTTGTAATCCTT
TTATCATGAATTACTTAAGGAAACTATCAGCTATCAATCATATAACGCGAGCATTATCAA
TAACATCGCGAAAAAATTCACATTTTTCACCGTTAACAGTTTTATCAGACGATGAACTTA
TGATGAAAGAGACATTTGCCAAGTTTGCAAGGGAAAAAATTGCACCAAAAGTCAGAGAAA
TGGAACAAAACCATAAACAAGATCCAGAAATCATTAAAGGATTATTTGAAAATGGTTTTA
TGGGAATTGAAATTGGTGAGGAATATGGTGGTTCTGGCTTGAATTTTATGTGTTCAATCT
TAGCGGTTGAAGAATTATCTAAAGTCGATAATTCGATTTCCGTTCTTGTTGATATACAAA
ACACACTTATAAATGCATTGATAGCAAAATTAGGCACACCTGAACAGAAGAAAAAATATC
TTACAAAATTAGCTACAAGTTCAGTTGGCAGTTTTGCATTGTCAGAACCTTCATCAGGAT
CAGATGCATTTGCTATGAAAACAACTGCAAAGAAAGATGGAAATCATTATGTTATCAATG
GCACAAAAATGTGGATTTCCAACTCAGATTTGGCTGATGTGTTTCTACTCATGGCAAATG
CTGATCCTTCAAAGGGTTATAAAGGTATTACGACATTTATTTTAGAGAAGAATATGGAAG
GCTTATCGATTGGCAAAAAGGAAGATAAATTAGGATTAGTTGCTTCAGGAACATGCATGA
TTCATCTTGATAATGTGCGAGTGCCAGAAGAAAACATTTTGGGTGAATTTGGAAAAGGAT
ATGCATATGCTGCAGGTTTTTTAAATGAAGGCAGAATCGGAATTGGTGCTCAAATGGTTG
GTTGTGCACAAGGTGCATTTGATGCTACTATCCCATATCTTCTTGAGCGTAAACAATTTG
GACAAAACATTTACGATTTTCAAGGAATGCAATTTCAAATTGCTGAAGTCGCAACACAAA
TTGAAGCAGGAAGATTATTAACTTATAATGCAGCTCGCTTGCAAGAAGCAGGTAAACCAT
TTTTAAAAGAGGCAGCAATGGCAAAACTCTTTTGCTCAAGAATGGCACAATTTGTTAGCA
GTCAATGTGTTGATTGGATGGGTGGTGTCGGATTTACAAAAGATTTTCCACAAGAGAAAT
TTTACAGAGATTCCAAAGCTAGTACAATTTATGAGGGTACAAGTAATATTCAAATGAGTA
CAATTGCTAAAATTATTAGAAAAGAATATTCTTCATGA
>g12935.t21 Gene=g12935 Length=410
MNYLRKLSAINHITRALSITSRKNSHFSPLTVLSDDELMMKETFAKFAREKIAPKVREME
QNHKQDPEIIKGLFENGFMGIEIGEEYGGSGLNFMCSILAVEELSKVDNSISVLVDIQNT
LINALIAKLGTPEQKKKYLTKLATSSVGSFALSEPSSGSDAFAMKTTAKKDGNHYVINGT
KMWISNSDLADVFLLMANADPSKGYKGITTFILEKNMEGLSIGKKEDKLGLVASGTCMIH
LDNVRVPEENILGEFGKGYAYAAGFLNEGRIGIGAQMVGCAQGAFDATIPYLLERKQFGQ
NIYDFQGMQFQIAEVATQIEAGRLLTYNAARLQEAGKPFLKEAAMAKLFCSRMAQFVSSQ
CVDWMGGVGFTKDFPQEKFYRDSKASTIYEGTSNIQMSTIAKIIRKEYSS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g12935.t21 | CDD | cd01158 | SCAD_SBCAD | 35 | 406 | 0.0 |
| 10 | g12935.t21 | Gene3D | G3DSA:1.10.540.10 | - | 23 | 147 | 3.0E-32 |
| 11 | g12935.t21 | Gene3D | G3DSA:2.40.110.10 | - | 148 | 259 | 3.8E-41 |
| 12 | g12935.t21 | Gene3D | G3DSA:1.20.140.10 | - | 260 | 408 | 7.0E-52 |
| 4 | g12935.t21 | PANTHER | PTHR43884:SF1 | SHORT/BRANCHED CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL | 17 | 408 | 7.8E-193 |
| 5 | g12935.t21 | PANTHER | PTHR43884 | ACYL-COA DEHYDROGENASE | 17 | 408 | 7.8E-193 |
| 13 | g12935.t21 | PIRSF | PIRSF016578 | PIGM | 28 | 406 | 2.5E-22 |
| 3 | g12935.t21 | Pfam | PF02771 | Acyl-CoA dehydrogenase, N-terminal domain | 34 | 144 | 1.1E-28 |
| 1 | g12935.t21 | Pfam | PF02770 | Acyl-CoA dehydrogenase, middle domain | 149 | 244 | 8.5E-25 |
| 2 | g12935.t21 | Pfam | PF00441 | Acyl-CoA dehydrogenase, C-terminal domain | 256 | 402 | 5.7E-46 |
| 9 | g12935.t21 | ProSitePatterns | PS00072 | Acyl-CoA dehydrogenases signature 1. | 151 | 163 | - |
| 8 | g12935.t21 | ProSitePatterns | PS00073 | Acyl-CoA dehydrogenases signature 2. | 363 | 382 | - |
| 6 | g12935.t21 | SUPERFAMILY | SSF56645 | Acyl-CoA dehydrogenase NM domain-like | 32 | 269 | 1.44E-75 |
| 7 | g12935.t21 | SUPERFAMILY | SSF47203 | Acyl-CoA dehydrogenase C-terminal domain-like | 254 | 408 | 4.83E-52 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016627 | oxidoreductase activity, acting on the CH-CH group of donors | MF |
| GO:0050660 | flavin adenine dinucleotide binding | MF |
| GO:0003995 | acyl-CoA dehydrogenase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K09478 (short-chain 2-methylacyl-CoA dehydrogenase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
