General information for g13000.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Pickpocket protein 28.
Parent gene
- The parent gene g13000 is located on chr_1: 27434217..27436069 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g13000 | g13000.t1 | isoform | g13000.t1 | 27434217 | 27436069 |
| chr_1 | g13000 | g13000.t1 | exon | g13000.t1.exon1 | 27434217 | 27434334 |
| chr_1 | g13000 | g13000.t1 | cds | g13000.t1.CDS1 | 27434217 | 27434334 |
| chr_1 | g13000 | g13000.t1 | exon | g13000.t1.exon2 | 27434400 | 27434580 |
| chr_1 | g13000 | g13000.t1 | cds | g13000.t1.CDS2 | 27434400 | 27434580 |
| chr_1 | g13000 | g13000.t1 | exon | g13000.t1.exon3 | 27434636 | 27434721 |
| chr_1 | g13000 | g13000.t1 | cds | g13000.t1.CDS3 | 27434636 | 27434721 |
| chr_1 | g13000 | g13000.t1 | exon | g13000.t1.exon4 | 27434778 | 27436069 |
| chr_1 | g13000 | g13000.t1 | cds | g13000.t1.CDS4 | 27434778 | 27436069 |
| chr_1 | g13000 | g13000.t1 | TSS | g13000.t1 | NA | NA |
| chr_1 | g13000 | g13000.t1 | TTS | g13000.t1 | NA | NA |
Sequences
>g13000.t1 Gene=g13000 Length=1677
ATGGTAATTTCACTAATAAAACTTATTACTCAGGAAAGCTCCATGCATGGACTTCGTCAC
ATCACTGACGATAGAAGAGATAAATTCTCAAAGATTTTCTGGTTATGTGTTGTCATAACT
TCAATTTGTTTGCTATTTCTCTCTGGTTACAATGTTTATTTTAAATGGAATATTGCACCC
GAAATTGGACTGCGAGTGAATCAAAAATCAATGGCAGATTTGCCTTTTCCTGCTATAACT
ATTTGTTCATCTGTCCTCGCTCGCGATAATTTGATTAATCTTACAAAGGCAATAAATTCT
GTAAAGGCAGGTCAACCAATAATGTACACACCGAAAGAGCAAAATTATTTTGCTGCCAAC
AGTCACATTTGCATCCCAGACCATATGAAAAAAGTAAATGACATTTGCAAAAATAGAACA
GAATTTAATATCATGAAATTGATAAATGAAAGTTCTTTAAATACTGGTGAAATGTTGAAA
AGCTGTTCTTTTAGAGAGATATCTTTTGATTGTGAAAAATTATTTACAAGAAGTATCACT
GATTATGGTTATTGTCATACATTCAACATGCTTGATCATGATCGAATTTTTAATCAAGAT
ACTATCAGCTCCGATTTTGATGTTTTTAGGAAAACAAATCAACTTGGCATCAATATGAGC
CTTCAATGGACATTGGATGAAAACTATATCGTTAATAGAAGTCAAGATATTTTGTTTCCC
GTCAGATCATTAAAGAAGAATATTCTGATTTTCTATACTTATCTTAATGAGACAGACAGC
ATTAATACTTGTCAACAAATCGGAAAAGTTTTTCCCATCATTCTTCACATGCCTAACGAA
ATACCGACACCATTTCATGAGGAAATGTATTTAGAATATGGTCGAAGGAAACAAGTAACA
ATTATGTTAAAAACTTACAAATCAAGTAATGATTTAAAGAAGTTCAGACCTAAGAAAAGA
GGTTGTTACTTTGAAGGTGAAAGGCAATTGAAATTTTTCAAGTCCTACACAAAGGCTCAT
TGTGATTTCGAATGCATGACTAATTATACTTTGAAAGTTTGTGGTTGTGTGAAATTTTCA
ATGCCAAGAACAAAGGGGACACCAATTTGTGACATTGATAAAGGCGACTGTTACTACAAT
GCTATGCTGCAATGGCCAAAAGATGGTGAAAATTCACATAGACCATGCAATTGTCTTGAA
ACGTGCAATTCAATCAAGTATAATATTCTCTACTCTAAAGATTCTTTTTTTAGTGATTAC
ACTGGACCATCACCTTTAATGGACATGAAACGTGGAACTTACAGTGTTTTTTTGATAAGG
ACCAGAAATCATGTGATTGATGAATATGAAAATTTTGCTGAATATCGTTTACAAAACTTT
GTTGCTGATTTTGGAGGACTTATGGGCTTATTTTTGGGTTGCAGTATTATATCATTGGTT
GAGTTGATTTACTTCTGTATAACAGGAACATTTAAGAAAATAAATTTTAAAAGAATTTCT
TTTGGGAGGAAGATAAATAACATTCCATTGACAAACTCGAAATTGCAACAGTTTCCAAAG
AAATTTTCACCAAAAAACCGTCCACTTAAGTCAAATGCAGATATGTGGCGTACATTGGAT
CAACTAAACTCAGATTTCATTTTACATCAACGAACTTCACAAAGAGTTAAATTTTAA
>g13000.t1 Gene=g13000 Length=558
MVISLIKLITQESSMHGLRHITDDRRDKFSKIFWLCVVITSICLLFLSGYNVYFKWNIAP
EIGLRVNQKSMADLPFPAITICSSVLARDNLINLTKAINSVKAGQPIMYTPKEQNYFAAN
SHICIPDHMKKVNDICKNRTEFNIMKLINESSLNTGEMLKSCSFREISFDCEKLFTRSIT
DYGYCHTFNMLDHDRIFNQDTISSDFDVFRKTNQLGINMSLQWTLDENYIVNRSQDILFP
VRSLKKNILIFYTYLNETDSINTCQQIGKVFPIILHMPNEIPTPFHEEMYLEYGRRKQVT
IMLKTYKSSNDLKKFRPKKRGCYFEGERQLKFFKSYTKAHCDFECMTNYTLKVCGCVKFS
MPRTKGTPICDIDKGDCYYNAMLQWPKDGENSHRPCNCLETCNSIKYNILYSKDSFFSDY
TGPSPLMDMKRGTYSVFLIRTRNHVIDEYENFAEYRLQNFVADFGGLMGLFLGCSIISLV
ELIYFCITGTFKKINFKRISFGRKINNIPLTNSKLQQFPKKFSPKNRPLKSNADMWRTLD
QLNSDFILHQRTSQRVKF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g13000.t1 | Gene3D | G3DSA:1.10.287.770 | - | 31 | 483 | 2.0E-47 |
| 11 | g13000.t1 | Gene3D | G3DSA:2.60.470.10 | - | 62 | 453 | 2.0E-47 |
| 12 | g13000.t1 | Gene3D | G3DSA:1.10.3590.10 | - | 85 | 268 | 2.0E-47 |
| 13 | g13000.t1 | Gene3D | G3DSA:1.10.287.820 | - | 314 | 402 | 2.0E-47 |
| 2 | g13000.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 10 | 497 | 7.3E-52 |
| 3 | g13000.t1 | PANTHER | PTHR11690:SF243 | PICKPOCKET 12-RELATED | 10 | 497 | 7.3E-52 |
| 4 | g13000.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 31 | 48 | 2.6E-9 |
| 5 | g13000.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 72 | 88 | 2.6E-9 |
| 8 | g13000.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 173 | 189 | 2.6E-9 |
| 7 | g13000.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 451 | 465 | 2.6E-9 |
| 6 | g13000.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 465 | 481 | 2.6E-9 |
| 1 | g13000.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 11 | 485 | 3.4E-72 |
| 15 | g13000.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 31 | - |
| 19 | g13000.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 32 | 53 | - |
| 17 | g13000.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 54 | 463 | - |
| 18 | g13000.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 464 | 487 | - |
| 16 | g13000.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 488 | 558 | - |
| 9 | g13000.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 32 | 54 | - |
| 10 | g13000.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 466 | 488 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005272 | sodium channel activity | MF |
| GO:0006814 | sodium ion transport | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed