Gene loci information

Transcript annotation

  • This transcript has been annotated as Cytochrome P450 9e2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g13107 g13107.t15 TTS g13107.t15 27990737 27990737
chr_1 g13107 g13107.t15 isoform g13107.t15 27990978 27993083
chr_1 g13107 g13107.t15 exon g13107.t15.exon1 27990978 27991985
chr_1 g13107 g13107.t15 cds g13107.t15.CDS1 27990978 27991985
chr_1 g13107 g13107.t15 exon g13107.t15.exon2 27992042 27992246
chr_1 g13107 g13107.t15 cds g13107.t15.CDS2 27992042 27992200
chr_1 g13107 g13107.t15 exon g13107.t15.exon3 27992413 27992591
chr_1 g13107 g13107.t15 exon g13107.t15.exon4 27992658 27992869
chr_1 g13107 g13107.t15 exon g13107.t15.exon5 27993034 27993083
chr_1 g13107 g13107.t15 TSS g13107.t15 27993083 27993083

Sequences

>g13107.t15 Gene=g13107 Length=1654
AGATCGAGTGAAGTCGCGAAGGCGAGTGGTTGATAATTTACTTTGCTGAATTTGTCATAA
TGATTATTTGGATTTTATTATTGATTGCCGCAGTCATTTTGCTGTACAAATATGGAACGA
GAAATTTCGATTACTTTCAAAAGAAAGGACTGGCTTTTAATAAGCCTCATTTTCTTGTTG
GGTCACGCCTAAGTACAGTTCTTGGTACAAAAAATATGATCGAAATGCTCACTGAAATTT
ATAATGAGCATAGAGATGAAAAAATTTCCGGCATGTTTGAATTCAATCGTCCAGCATATT
TTATTCGTGACCCAGAGATGATTAAACGACTTTGTATTAAAGAATTTGATTCATTCAGCG
ATCATCGACTTATTCTTTCTAAAGAAGCAGAACCTCTCTTTGCTTCTGCACTTTTCGGTT
TGACGGGTCAAAAATGGAGAGAAATGTGATGAAAACTTTTTCTTTTTCATACACTTTAGG
CAGCAAAATGCGTCTAATGTTTAAGCTCATAAACGACGTTGGCTCTCAAATGTCGAATAC
TGTTCTTGAACAAATAAATAAAGGAGCTGACAACAAAATTGAGTTTAAAGAATTTGCTCA
GAAATTTACAATCGATATTATAGCAAGTTGCGCATTTGGATTAGAGATAAATTCATTCAA
TAATCCCAATAATGACTTCCTGAGAATTGCAAGAAAAGCAACAAATTTCGCTACCTTTAA
GACCACAATAAAATTAGTGGGTTTACTTGGTGCACCATGGTTAATGAAAATATTTAAAAT
CAAGTTTTTGGATGAAGAATTTTATAAATTCTTTGGTGCAATGATGACAGATACTATAAA
AACTAGAGAAGAGAAGAAAATAACTCGTAATGATATGATCGATTTGATATTGCAAGCAAA
ACACGGTAAACAACTTGATTATGACAATGAAGAGCATACAGTTGATGGTTTCGCAACAGT
TGAGGAATCACAATTAGGAAAAGAAAAAGTTAAGAGAACATGGGAAGATGATGAACTTTT
AGCACAATGTGTTGGCTTCTTTTTTGGTGGATTTGAAACAGTTTCGAGTGTCATGACTTT
TATGGCTGCTGAATTGATTATTGATCCTAATATTCAGAAAAAATTACAAGACGAAATTGA
TGAAGTTCACAAGTCATTGAACGGAGAAACTTTGACATACGAGCATGTACAAAAAATGAA
ATACATGGATATGGTTGTTTGCGAGACTCTACGAAAATGGCCACCTGCACCAATTGTTGA
TAGAAAGTGCACTAAAGAATTTAATTTCGAATATGATGACAAAAAATTCATTATGGAATA
TGATAAAAATTTCTATGTTCCTATCTATTCATTACATCATGATGAACGTTATTTCCCAGA
ACCAGAAAAATTCGATCCTGAACGATTTAATGATGAAAATAAGAAAAATATCAGACAAGA
TTGTTATATACCTTTTGGTATTGGTCCAAGAAATTGCATTGGAAACCGTTTTGCTTTACT
TGAAGTGAAAACAATTTTTTACTACCTCCTATTAAACTTTACATTTGAAGCGACAAATGA
AACTGAAATTCCCCTCAAATTGGGAAGAAGTCAAACAACATTACAACTTGAAAATGGATT
GAAATGTGCCTTGATTCCACGAGCTGAAGAATAA

>g13107.t15 Gene=g13107 Length=388
MRLMFKLINDVGSQMSNTVLEQINKGADNKIEFKEFAQKFTIDIIASCAFGLEINSFNNP
NNDFLRIARKATNFATFKTTIKLVGLLGAPWLMKIFKIKFLDEEFYKFFGAMMTDTIKTR
EEKKITRNDMIDLILQAKHGKQLDYDNEEHTVDGFATVEESQLGKEKVKRTWEDDELLAQ
CVGFFFGGFETVSSVMTFMAAELIIDPNIQKKLQDEIDEVHKSLNGETLTYEHVQKMKYM
DMVVCETLRKWPPAPIVDRKCTKEFNFEYDDKKFIMEYDKNFYVPIYSLHHDERYFPEPE
KFDPERFNDENKKNIRQDCYIPFGIGPRNCIGNRFALLEVKTIFYYLLLNFTFEATNETE
IPLKLGRSQTTLQLENGLKCALIPRAEE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
15 g13107.t15 Gene3D G3DSA:1.10.630.10 Cytochrome p450 1 388 3.0E-97
2 g13107.t15 PANTHER PTHR24292 CYTOCHROME P450 1 368 3.1E-136
3 g13107.t15 PANTHER PTHR24292:SF90 CYTOCHROME P450 317A1-RELATED 1 368 3.1E-136
7 g13107.t15 PRINTS PR00463 E-class P450 group I signature 176 193 1.5E-22
11 g13107.t15 PRINTS PR00385 P450 superfamily signature 187 204 1.1E-9
8 g13107.t15 PRINTS PR00463 E-class P450 group I signature 196 222 1.5E-22
10 g13107.t15 PRINTS PR00385 P450 superfamily signature 242 253 1.1E-9
6 g13107.t15 PRINTS PR00463 E-class P450 group I signature 285 309 1.5E-22
5 g13107.t15 PRINTS PR00463 E-class P450 group I signature 320 330 1.5E-22
9 g13107.t15 PRINTS PR00385 P450 superfamily signature 321 330 1.1E-9
4 g13107.t15 PRINTS PR00463 E-class P450 group I signature 330 353 1.5E-22
12 g13107.t15 PRINTS PR00385 P450 superfamily signature 330 341 1.1E-9
1 g13107.t15 Pfam PF00067 Cytochrome P450 8 371 5.1E-68
14 g13107.t15 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 323 332 -
13 g13107.t15 SUPERFAMILY SSF48264 Cytochrome P450 2 385 1.14E-86

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values