Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Cytochrome P450 9e2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g13107 g13107.t3 isoform g13107.t3 27990577 27991463
chr_1 g13107 g13107.t3 exon g13107.t3.exon1 27990577 27991463
chr_1 g13107 g13107.t3 cds g13107.t3.CDS1 27990978 27991436
chr_1 g13107 g13107.t3 TSS g13107.t3 NA NA
chr_1 g13107 g13107.t3 TTS g13107.t3 NA NA

Sequences

>g13107.t3 Gene=g13107 Length=887
ACTTTGACATACGAGCATGTACAAAAAATGAAATACATGGATATGGTTGTTTGCGAGACT
CTACGAAAATGGCCACCTGCACCAATTGTTGATAGAAAGTGCACTAAAGAATTTAATTTC
GAATATGATGACAAAAAATTCATTATGGAATATGATAAAAATTTCTATGTTCCTATCTAT
TCATTACATCATGATGAACGTTATTTCCCAGAACCAGAAAAATTCGATCCTGAACGATTT
AATGATGAAAATAAGAAAAATATCAGACAAGATTGTTATATACCTTTTGGTATTGGTCCA
AGAAATTGCATTGGAAACCGTTTTGCTTTACTTGAAGTGAAAACAATTTTTTACTACCTC
CTATTAAACTTTACATTTGAAGCGACAAATGAAACTGAAATTCCCCTCAAATTGGGAAGA
AGTCAAACAACATTACAACTTGAAAATGGATTGAAATGTGCCTTGATTCCACGAGCTGAA
GAATAATATTTATGTCTTTTAAATATCTTTGATTAATAATAAGCAATTAAACATTTAATG
ACGTTTGCATACACAATTCACTATGGCCATCTATCTAATAAAATTTATTATCACAAAGGA
TAATTTTAATTACGATACTAATTATTAATGCTACTCATAACTATTTTCATGTAAAATTAC
TAAGAAGTATAAAAGCACAATGCTTTAAAATAAAGCTAATCAGTTTATATACTAACTCGT
TGTAATCAAAACTATTTTGAGAAATTTTTATTTTTTTCTGTTCGTCAATGTGGAATTAAA
ATTCATCCAATAAATTTGAATGCTTCAAAGAATAATTTTTTTAATCGTAATATAATAAAA
ACGTCTCAAAAATTTCAAAGCAATTTCCTTTTAAGAGTTGCAGTAAA

>g13107.t3 Gene=g13107 Length=152
MKYMDMVVCETLRKWPPAPIVDRKCTKEFNFEYDDKKFIMEYDKNFYVPIYSLHHDERYF
PEPEKFDPERFNDENKKNIRQDCYIPFGIGPRNCIGNRFALLEVKTIFYYLLLNFTFEAT
NETEIPLKLGRSQTTLQLENGLKCALIPRAEE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
13 g13107.t3 Gene3D G3DSA:1.10.630.10 Cytochrome p450 1 152 4.5E-49
2 g13107.t3 PANTHER PTHR24292 CYTOCHROME P450 1 132 4.7E-61
3 g13107.t3 PANTHER PTHR24292:SF90 CYTOCHROME P450 317A1-RELATED 1 132 4.7E-61
10 g13107.t3 PRINTS PR00465 E-class P450 group IV signature 1 13 5.2E-16
5 g13107.t3 PRINTS PR00385 P450 superfamily signature 6 17 3.4E-6
8 g13107.t3 PRINTS PR00465 E-class P450 group IV signature 54 72 5.2E-16
7 g13107.t3 PRINTS PR00465 E-class P450 group IV signature 78 94 5.2E-16
6 g13107.t3 PRINTS PR00385 P450 superfamily signature 85 94 3.4E-6
4 g13107.t3 PRINTS PR00385 P450 superfamily signature 94 105 3.4E-6
9 g13107.t3 PRINTS PR00465 E-class P450 group IV signature 94 112 5.2E-16
1 g13107.t3 Pfam PF00067 Cytochrome P450 1 136 3.8E-40
12 g13107.t3 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 87 96 -
11 g13107.t3 SUPERFAMILY SSF48264 Cytochrome P450 1 149 2.62E-46

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0004497 monooxygenase activity MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values