General information for g13151.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Exostosin-1.
Parent gene
- The parent gene g13151 is located on chr_1: 28258894..28261422 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g13151 | g13151.t1 | TTS | g13151.t1 | 28258516 | 28258516 |
| chr_1 | g13151 | g13151.t1 | isoform | g13151.t1 | 28258894 | 28261422 |
| chr_1 | g13151 | g13151.t1 | exon | g13151.t1.exon1 | 28258894 | 28259073 |
| chr_1 | g13151 | g13151.t1 | cds | g13151.t1.CDS1 | 28258894 | 28259073 |
| chr_1 | g13151 | g13151.t1 | exon | g13151.t1.exon2 | 28259215 | 28261422 |
| chr_1 | g13151 | g13151.t1 | cds | g13151.t1.CDS2 | 28259215 | 28261422 |
| chr_1 | g13151 | g13151.t1 | TSS | g13151.t1 | 28261946 | 28261946 |
Sequences
>g13151.t1 Gene=g13151 Length=2388
ATGCAGGCCAAAAAACGATATATATTGGTCGTGCTCTCTTGTGCATTCTTAGCCTATTGC
TATTTCGGAGGCTACCGAGTAAAAAAGTTATTCAATCTCAACACAAACACATTCGACAAC
TTACCGTCATTTATCATAAGTCAAAATCGAATAAATTATCATAGTAGTTTAAGTGTGAAA
GCAAATAATCAATTTAACAACATTATCTTACATAAGTATGAAAATGAAAACAGTAGAAGT
AAGCATTTCAAGGATATAGATGATCTCTATGAAACGCCCGAATACAACCGCTTACGAACG
GATCATCAGAGATCAATGAATAGTAATAGTAACAATAATAATAATTTTAATTCATGTACA
ATGGAGACATGCTTTGATTTTACAAAATGCTATGATGATTTCTTAGTGTATGTATATCCT
TCAGAGCCATTAAATACGCTTGGCACAACAACACCACCAGTGAGTGCAAATTATCAGAAA
ATTTTGACTGCAATCACAGAATCACGATATTACACAAGTGACCCCGAAAAAGCATGTCTA
TTTGTTCTAGCAATTGATACACTCGATAGAGATTCTCTAAGTGAGGACCTAGTGCGTAAT
GTTCCACAACGATTGCAGAGACTACCGTATTGGAATAATGGCAAGAATCACATAATATTT
AATCTCTATTCAGGCACTTGGCCATCATATCATGAACTCGGATTGGGTTTCGATCCAGGT
CAAGCTATACTTGCAAAAGCCAGCATGAGTATTCAAACAATGCGGCATGGCTTCGATGTT
AGCATTCCATTGTTTCATAAACACTTTCCATTACGAAGTGGACATACGGGAATGATTACG
GCAAATAATTTCCCTGTCAATAAGAAGCATTTATTAGCCTTCAAGGGAAAACGTTATGTG
CATGGAATTGGATCGGAGACGAGAAACTCATTGTTTCATTTACACAATGGTAGAGATATT
GTCATGGTAACAACATGTAGACATGGAAAGAGTTGGAGAGAGTTACAGGATGGACGTTGT
GATGAGGATAATCGAGAGTATGACCGTTACGATTATGAGACTCTTTTACAAAACTCAACA
TTTTGTCTTGTGCCGCGTGGAAGACGTCTAGGTTCATTTCGTTTTTTAGAGGCACTTCAA
GCAGGTTGCATTCCAGTGCTCTTATCAAATTCATGGGTATTGCCATTTCATCCGCATATT
GATTGGAAACAAGCCGCGATTTGGGGCGACGAGAGACTGCTACTTCAAGTTGTTGATATC
GTTCGATCAATTAGTCCATCAAAAATTATGGCGCTTCGCCAACAGACACAAATTTTATGG
GAACGCTATTTCAGCTCAATAGAGAAAATCGTATTTACGACATTTGAAATTATTCGCGAA
CGCCTTCCCGACTATCCTGTAAGAAATGGATTGATATGGAATAGCTCGCCAGGTGCTTTA
CTAACATTACCAACATTCTCCGATAATTTAAGAGATTTGCCATTTTATCTCGATCGATTA
GGCTATGAGCCACGAGCAAATTTTACGGCTGTCATTTTTGTACAAGTCGGTCAACCTATC
AATCCTCAATCAGCCCTCTATAAGCTAGTCAAAAGCATAACAAAGAGTCAATTTGTAAAT
AGAATATTAATACTGTGGGCAGCAGATCAAAAAGTGCCATCACGCAAGAGATTTCCTGCG
ACTGGACATGTGCCATTACATATAGTGACATCACTAAATATACCGCAAATGCAAACACAT
TCATCACTTAACATTGCAGCAGGAGCAACAGATGAACGTCCAAGCATATCACAGCGTTTT
CAGCCACATCCACTAATTGAGACAGATGCCATTCTGTCACTTGATGAAGATGCCATACTC
AACACAGAAGAGATTGATTTCGCATACAATGTGTGGAAATTTTTTCCTGAGCGCATTGTT
GGCTTTCCTGCTCGAGCACATTTTTACGATGATTCAAAAAATACATGGGGCTATACGTCT
AAGTGGACAAACTATTATTCAATTGTTTTAACTGGTGCAGCGTTTTATCATCGCTATTAT
AATTATTTATATACAAATTTTCTTTCGCATTTACTTTTGAAGACTGTGCAGCAATCATCC
AATTGTGAAGACATTCTCATGAACATGATGGTATCGCATGTTACTCGAAAACCGCCAATT
AAAGTGACACAGCGCAAGGCATATAAAGATCGTGATAGTGTACGAAGTCCATGGAATGAT
CCAGATCATTTTATTCAACGTCAAAGCTGCCTCAATACATTTGTTGCAGTTTTTGGTTAC
ATGCCATTATTGAAATCGAATATGCGTCTCGATCCAGTACTCTATAAGGATGCAGTTTCA
AATTTGCGCAAAAAATATAGACAAATTGAATTAGTGGGTAAGAGATAG
>g13151.t1 Gene=g13151 Length=795
MQAKKRYILVVLSCAFLAYCYFGGYRVKKLFNLNTNTFDNLPSFIISQNRINYHSSLSVK
ANNQFNNIILHKYENENSRSKHFKDIDDLYETPEYNRLRTDHQRSMNSNSNNNNNFNSCT
METCFDFTKCYDDFLVYVYPSEPLNTLGTTTPPVSANYQKILTAITESRYYTSDPEKACL
FVLAIDTLDRDSLSEDLVRNVPQRLQRLPYWNNGKNHIIFNLYSGTWPSYHELGLGFDPG
QAILAKASMSIQTMRHGFDVSIPLFHKHFPLRSGHTGMITANNFPVNKKHLLAFKGKRYV
HGIGSETRNSLFHLHNGRDIVMVTTCRHGKSWRELQDGRCDEDNREYDRYDYETLLQNST
FCLVPRGRRLGSFRFLEALQAGCIPVLLSNSWVLPFHPHIDWKQAAIWGDERLLLQVVDI
VRSISPSKIMALRQQTQILWERYFSSIEKIVFTTFEIIRERLPDYPVRNGLIWNSSPGAL
LTLPTFSDNLRDLPFYLDRLGYEPRANFTAVIFVQVGQPINPQSALYKLVKSITKSQFVN
RILILWAADQKVPSRKRFPATGHVPLHIVTSLNIPQMQTHSSLNIAAGATDERPSISQRF
QPHPLIETDAILSLDEDAILNTEEIDFAYNVWKFFPERIVGFPARAHFYDDSKNTWGYTS
KWTNYYSIVLTGAAFYHRYYNYLYTNFLSHLLLKTVQQSSNCEDILMNMMVSHVTRKPPI
KVTQRKAYKDRDSVRSPWNDPDHFIQRQSCLNTFVAVFGYMPLLKSNMRLDPVLYKDAVS
NLRKKYRQIELVGKR
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g13151.t1 | Gene3D | G3DSA:3.90.550.10 | Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A | 506 | 785 | 1.6E-73 |
| 3 | g13151.t1 | PANTHER | PTHR11062:SF129 | EXOSTOSIN-1 | 3 | 724 | 6.5E-168 |
| 4 | g13151.t1 | PANTHER | PTHR11062 | EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED | 3 | 724 | 6.5E-168 |
| 2 | g13151.t1 | Pfam | PF03016 | Exostosin family | 133 | 424 | 8.1E-54 |
| 1 | g13151.t1 | Pfam | PF09258 | Glycosyl transferase family 64 domain | 508 | 775 | 1.3E-94 |
| 9 | g13151.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 10 | g13151.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 7 | - |
| 11 | g13151.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 8 | 16 | - |
| 12 | g13151.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 17 | 20 | - |
| 8 | g13151.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 795 | - |
| 6 | g13151.t1 | SUPERFAMILY | SSF53448 | Nucleotide-diphospho-sugar transferases | 521 | 777 | 6.75E-18 |
| 5 | g13151.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 25 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006486 | protein glycosylation | BP |
| GO:0016757 | glycosyltransferase activity | MF |
| GO:0016021 | integral component of membrane | CC |
KEGG
Orthology
- This transcript has been annotated with K02366 (glucuronyl/N-acetylglucosaminyl transferase EXT1)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
