General information for g13310.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Orexin receptor type 2.
Parent gene
- The parent gene g13310 is located on chr_1: 29071901..29077877 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g13310 | g13310.t1 | TTS | g13310.t1 | 29071592 | 29071592 |
| chr_1 | g13310 | g13310.t1 | isoform | g13310.t1 | 29071901 | 29077877 |
| chr_1 | g13310 | g13310.t1 | exon | g13310.t1.exon1 | 29071901 | 29072020 |
| chr_1 | g13310 | g13310.t1 | cds | g13310.t1.CDS1 | 29071901 | 29072020 |
| chr_1 | g13310 | g13310.t1 | exon | g13310.t1.exon2 | 29072077 | 29072273 |
| chr_1 | g13310 | g13310.t1 | cds | g13310.t1.CDS2 | 29072077 | 29072273 |
| chr_1 | g13310 | g13310.t1 | exon | g13310.t1.exon3 | 29072340 | 29072591 |
| chr_1 | g13310 | g13310.t1 | cds | g13310.t1.CDS3 | 29072340 | 29072591 |
| chr_1 | g13310 | g13310.t1 | exon | g13310.t1.exon4 | 29073230 | 29073345 |
| chr_1 | g13310 | g13310.t1 | cds | g13310.t1.CDS4 | 29073230 | 29073345 |
| chr_1 | g13310 | g13310.t1 | exon | g13310.t1.exon5 | 29073418 | 29073704 |
| chr_1 | g13310 | g13310.t1 | cds | g13310.t1.CDS5 | 29073418 | 29073704 |
| chr_1 | g13310 | g13310.t1 | exon | g13310.t1.exon6 | 29075470 | 29075664 |
| chr_1 | g13310 | g13310.t1 | cds | g13310.t1.CDS6 | 29075470 | 29075664 |
| chr_1 | g13310 | g13310.t1 | exon | g13310.t1.exon7 | 29077614 | 29077877 |
| chr_1 | g13310 | g13310.t1 | cds | g13310.t1.CDS7 | 29077614 | 29077877 |
| chr_1 | g13310 | g13310.t1 | TSS | g13310.t1 | NA | NA |
Sequences
>g13310.t1 Gene=g13310 Length=1431
ATGGAAGTGCTATCAACACTCTCCACAATGAAACCACAAATAACAACAACAGCATCATCA
TCATTTGTGACAACAACCACAGAGCTCTTTTGGAATCATTCATCAACAGTAGATTATGGC
ACAAATCCAATTGTGACAACTTGCTCAGAGACTAACCCAAACTGTGTCATAGATCATTCA
GAACAGTGTGTTGGTGATCCAGCATACTGTAATCTCACTGAAAGTGAATACGTTCAGCTT
TTATATGATTATATTTCACCAACGATAGGAAATGCTTTAGTCTGTCTTGCTGTTTGGTCA
AATCATTCTATGCGTACTGTGACAAATATTTTCATAGTCAATTTGGCTGTAGCTGATTTC
TTCGTAATTCTTTTCTGCCTTCCACCAACCGTGATATGGGATGTGACAGAGACATGGTTC
CTAGGTGAAACAATGTGCAAAGTTGTAATTTATTTTCAGACTGTGAGCGTAACTGTATCA
ATTCTAACATTGACATTCATCTCAATCGATCGTTGGTACGCAATCGTCTTTCCTTTACGT
TATAAACCACAACCGGGTCGAGCTATAGTTTACATTGGAATCATTTGGGCTGTAGGATTA
CTTTTTGACTTGCCTGAATTTCTTGTTCTACATACAACAACAAAAAGACTTCGTTTTGAC
ATACAACTTTTTACACAGTGCGTCACATCATGGACCGTAGAGGAAGAGAAACGGTTCGTT
ATCATTAAAGCCATATTTCTCTACACACTTCCACTTATTCTCATGACAATCGCATACTGT
CAGATTGTAAGAGTGCTATGGCGATCTGATACGATCCCAGGACATAATAACTACAAAACA
CAGAAGCTTAATACTTATCGAGGTAAGGGTAGCGTAGGTGAATCAAATTCAACGACCATA
GCACAATTAAGGGCACGTCGTAAGGCGTCAAAGATGCTTGTCGCTGTCGTGATTATGTTC
GCAGCTTGCTATTTTCCTGTTCATGCACTTAATCTCATACGCTACACATACGATCTCAAT
CAAAATGAAATTATTTCGGTGCTTTCGTTGCTCTCTCACTGGCTGTGCTATGCTAATAGC
TGTGCAAATCCTGTTATTTATAACTTTATGAGCGGAAAATTTAGAAGAGAGTTCCGAAAT
GTTCTAGAACGTGGTCACTGTCTCACTTTGAAACATCATCAACGTCATAATCATTGGCAA
TCACAATATCGATCGAGATATGCAAATGATGACCAAGAACATTCATTTATTCATTCAACA
ACACATATGATTCATGTGACGCCTAGTTTGAAGAGAAATTATGGTTGCACACAAACTTGT
CAGACGTCATTTGCCGCCAATGGAGCAACTGTTTTGGGTCGAGATGTTGTTAGGAGTTCT
TTTCAAGATAGCCTCAAGACAATTCCATCAGTAGATGCGAAAGTAAATTAA
>g13310.t1 Gene=g13310 Length=476
MEVLSTLSTMKPQITTTASSSFVTTTTELFWNHSSTVDYGTNPIVTTCSETNPNCVIDHS
EQCVGDPAYCNLTESEYVQLLYDYISPTIGNALVCLAVWSNHSMRTVTNIFIVNLAVADF
FVILFCLPPTVIWDVTETWFLGETMCKVVIYFQTVSVTVSILTLTFISIDRWYAIVFPLR
YKPQPGRAIVYIGIIWAVGLLFDLPEFLVLHTTTKRLRFDIQLFTQCVTSWTVEEEKRFV
IIKAIFLYTLPLILMTIAYCQIVRVLWRSDTIPGHNNYKTQKLNTYRGKGSVGESNSTTI
AQLRARRKASKMLVAVVIMFAACYFPVHALNLIRYTYDLNQNEIISVLSLLSHWLCYANS
CANPVIYNFMSGKFRREFRNVLERGHCLTLKHHQRHNHWQSQYRSRYANDDQEHSFIHST
THMIHVTPSLKRNYGCTQTCQTSFAANGATVLGRDVVRSSFQDSLKTIPSVDAKVN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g13310.t1 | Gene3D | G3DSA:1.20.1070.10 | - | 79 | 427 | 4.4E-90 |
| 2 | g13310.t1 | PANTHER | PTHR45695 | LEUCOKININ RECEPTOR-RELATED | 88 | 405 | 1.4E-80 |
| 9 | g13310.t1 | PRINTS | PR01012 | Neuropeptide Y receptor signature | 100 | 112 | 1.2E-10 |
| 4 | g13310.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 108 | 129 | 2.5E-37 |
| 12 | g13310.t1 | PRINTS | PR01012 | Neuropeptide Y receptor signature | 130 | 142 | 1.2E-10 |
| 8 | g13310.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 153 | 175 | 2.5E-37 |
| 11 | g13310.t1 | PRINTS | PR01012 | Neuropeptide Y receptor signature | 153 | 168 | 1.2E-10 |
| 6 | g13310.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 187 | 208 | 2.5E-37 |
| 5 | g13310.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 240 | 263 | 2.5E-37 |
| 3 | g13310.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 309 | 333 | 2.5E-37 |
| 7 | g13310.t1 | PRINTS | PR00237 | Rhodopsin-like GPCR superfamily signature | 349 | 375 | 2.5E-37 |
| 10 | g13310.t1 | PRINTS | PR01012 | Neuropeptide Y receptor signature | 365 | 378 | 1.2E-10 |
| 1 | g13310.t1 | Pfam | PF00001 | 7 transmembrane receptor (rhodopsin family) | 90 | 367 | 7.8E-57 |
| 20 | g13310.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 80 | - |
| 29 | g13310.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 81 | 99 | - |
| 18 | g13310.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 100 | 110 | - |
| 26 | g13310.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 111 | 133 | - |
| 23 | g13310.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 134 | 147 | - |
| 27 | g13310.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 148 | 167 | - |
| 19 | g13310.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 168 | 187 | - |
| 25 | g13310.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 188 | 210 | - |
| 22 | g13310.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 211 | 239 | - |
| 24 | g13310.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 240 | 260 | - |
| 17 | g13310.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 261 | 311 | - |
| 30 | g13310.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 312 | 333 | - |
| 21 | g13310.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 334 | 344 | - |
| 28 | g13310.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 345 | 369 | - |
| 16 | g13310.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 370 | 476 | - |
| 37 | g13310.t1 | ProSitePatterns | PS00237 | G-protein coupled receptors family 1 signature. | 159 | 175 | - |
| 39 | g13310.t1 | ProSiteProfiles | PS50262 | G-protein coupled receptors family 1 profile. | 90 | 367 | 46.069 |
| 38 | g13310.t1 | SMART | SM01381 | 7TM_GPCR_Srsx_2 | 88 | 382 | 0.0027 |
| 13 | g13310.t1 | SUPERFAMILY | SSF81321 | Family A G protein-coupled receptor-like | 75 | 400 | 1.37E-68 |
| 14 | g13310.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 20 | - |
| 33 | g13310.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 111 | 133 | - |
| 35 | g13310.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 148 | 167 | - |
| 32 | g13310.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 188 | 210 | - |
| 34 | g13310.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 245 | 267 | - |
| 31 | g13310.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 313 | 335 | - |
| 36 | g13310.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 350 | 372 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0007186 | G protein-coupled receptor signaling pathway | BP |
| GO:0004983 | neuropeptide Y receptor activity | MF |
| GO:0016021 | integral component of membrane | CC |
| GO:0004930 | G protein-coupled receptor activity | MF |
KEGG
Orthology
- This transcript has been annotated with K04239 (hypocretin (orexin) receptor 2)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed