General information for g13476.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Fatty acid synthase.
Parent gene
- The parent gene g13476 is located on chr_1: 30381103..30385948 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g13476 | g13476.t1 | isoform | g13476.t1 | 30381103 | 30385948 |
| chr_1 | g13476 | g13476.t1 | exon | g13476.t1.exon1 | 30381103 | 30382798 |
| chr_1 | g13476 | g13476.t1 | cds | g13476.t1.CDS1 | 30381103 | 30382798 |
| chr_1 | g13476 | g13476.t1 | exon | g13476.t1.exon2 | 30382858 | 30383207 |
| chr_1 | g13476 | g13476.t1 | cds | g13476.t1.CDS2 | 30382858 | 30383207 |
| chr_1 | g13476 | g13476.t1 | exon | g13476.t1.exon3 | 30383272 | 30384672 |
| chr_1 | g13476 | g13476.t1 | cds | g13476.t1.CDS3 | 30383272 | 30384672 |
| chr_1 | g13476 | g13476.t1 | exon | g13476.t1.exon4 | 30384802 | 30385861 |
| chr_1 | g13476 | g13476.t1 | cds | g13476.t1.CDS4 | 30384802 | 30385861 |
| chr_1 | g13476 | g13476.t1 | exon | g13476.t1.exon5 | 30385920 | 30385948 |
| chr_1 | g13476 | g13476.t1 | cds | g13476.t1.CDS5 | 30385920 | 30385948 |
| chr_1 | g13476 | g13476.t1 | TSS | g13476.t1 | NA | NA |
| chr_1 | g13476 | g13476.t1 | TTS | g13476.t1 | NA | NA |
Sequences
>g13476.t1 Gene=g13476 Length=4536
ATGGAATTTTTCATAGAATCACTTGGAAAATTATTCCAACAAGGAGTTGACATGGATATT
TCAAAACTTTATCCACCTATTCAGTTCCCAGTTTCACGAAATACTCCAATGATTTCACCA
AATATTAAATGGAATCATACAGAAAATCATTTTGTTCCTTATTATGATTCTTTCAATTGG
TATGAGCGAAGAAATATCATAATTAATATTAATGACAAAGTTTTCGAACCAGTACAAGGA
CATTTAATTGACGGTCGATGCCTTTTCCCAGCAACTGGGTGGATCTTTTTAGTTTGGGAT
ACTTTTGCATCCATGAATGGAATGCCAGTTGATAAAGCTCGAGTTGTTTTTGAAGATATT
CAATTTTTACGAGCAACAGCATTGAATAAGAATCAAGATGTTTTGATAACAATTTCGATT
CATCGTGGAACAGGAAGGTTCGAGGTCATTGAAGGAGTTTCAGCTGTTGTTCATGGATAT
ATTAAACATGATGATGAAATAGAAATGTCAGAATTTACAGTTCCTAAAACTGACAATCCT
TTGAAATTAGAAACCGATGATTTCTACAAAGAGATGAGATTGAGTGGTTTTTGTCACTTA
AACAAACTTCAGGGAATTAAGGAAATCCGACATGATGGTCTTGAGGGAAAAATTAAATGG
GAAGATGACTGGATTGCTTTTATTGATAGTATGACACATTTTGAGGAATTTGAGACCAAA
TCAAAAATTCTTGCATTACCAACAAATATAAGAAAAATTATCATTGATCCAATTTTGCAT
CTTAAAATGTTAGAAGAAAAGAAGAAAGAGCTTGAAGGTGTTGAAGATGACAAAAAAGAA
ATTTTATACGATGCGAAAATTTCACCTTACTTGAGAATTATTCAAGCAGGTGGTGTTGAA
ATTCATGAAAAAAAGAATCGTGTAGTAAACCGGAGAAGACCAAAAGAACCAACACTTGAA
AGTCAAAAATTCATTCCATTTTTTGCTAATGAAAAATTTTCATTGAAAGATGCTGCAAAA
ATTATCACACAAACGGCTCTTGAGACAATCTTTCAACCAGATTTTGCTGCATTGAAATTG
ATTCTGATACTTTCATCATTCAATGGAGTTGATTTGATTAATAAAAGCAATTGTGTAAAT
GATAACGAATTTTTGCATTCAATCAAATCAGTTTTAAATAGCAATGGTTACATTTTATCA
AGAGAGCAATCAACGGCTTCAGAAATTGATGAGAAACTTCAACTTGTTGCTAAGGTTTCA
CTCGAAAATGGTGAAGTTCTTCATATGCTTCAATTTAAAGACAATATCAAACTATTTGAA
CCACAAAATATAATCGAGATCACATCAAATGTCAGTGAATGGCTTGAACCCTTGAAATCA
GCTTTAAAAGAAGGGACAACACTTGTCTACTCATACAATCATGATTATTCAGGCATACTT
GGATTAGTTAATTGTGTGAGAAGAGAATATCCACCTGAAAGACTCAAATGCATTTTTCTA
ATCGATACAAAAACTGCACCAGCTATTTTTGACATTAATGATGAATTTTTTAAGTCACAG
CTACTGCTCAATCACACAATTAATGTTTATAAAAATCAATTATGGGGTGGTTATCGTTTC
TTAGATATCACAGAAGAAACTGACAAAAAACCTCAAAAAAATCATTGCTTTGCAAATTGT
ATCATAAGAGGTGATTTGTCTTCGCTTTCTTGGTTAAAAGGGCCATTTGATGTTCATGAT
TCAGCAAACAAAGATTTAATAAGAATTCAATATTCGTCGCTTAACTTCAAAGACGTAATG
TTGGCACTTGGAAGAATTCTCTATAAAGAATTCGATCGACTTGAAAGACAATGTGTACTT
GGTCTTGAATTTTCAGGTCTAAAAGCTGATAATACACGTGTAATGGGAATTGCTTTGAAA
GCTGGTGCATTAGCAACACATTATGACAGCAAATACTCAATTCTTTGGAATGTACCTGAT
AATTGGAGTTTAGAAGAAGCAGCGACTGTTCCATTGGTTTATTTCACTGTCTATTTTGCA
TTTTTTGTCACAACAAAAATCAAAAGTGGCAAAAGCATTTTAATTCATTCAGGAACCGGT
GGTGTGGGACAAGCTGCAATTCAAGTTGCTTTTGCTTATGGTCTTGAAGTTTTCACAACA
GTTGGCTCAGATGACAAGAAAGAATTTTTTACCCTTAAGAAGTTTCCAAAACTTAAACCT
GAAAATATTGGAAATTCAAGAAATACAACATTCGAGAGAATGATATTGGAAAATACTAAA
GGAAATGGAGTCGATTATGTTTTGAATTCTCTTGCTGATGACAAACTCAAAGCATCGATT
AGATGTCTCGCAAAAGACGGTACATTGCTTGAGATAGGCAAATATGATATGTTGAAGGGA
ACGAAATTAGACATGAGATATCTGACGAAGCGTATTAAGCTCAAGGCTATTCATTTTGAC
GACCTACCAACAGATTCAGACGAAATGAAGGCGGTTTACTCTCATATAGATAAAGATTTA
AAATCAGGTATAATTCAGCCACTAAACACGACAGTTTTTGATGCTAATGACGTTGAAGAG
GCTTTCCGATACATGGCATCAGGCAAGCATATGGGAAAAGTTCTCCTAAAAGTTAGGCAA
AATGAACATGATGAAAGTTCATTGCCCTTGATGGCATTGGATCGAGTGTTTGTTGATGCG
AATGAAACAGTTTTCATTGCTGGTGGTTTAGGAGGATTTGGAATGGAACTTGCTGATTGG
TTAATCCTTCGAGGATGTAGGAAAGTTATGTTAAGTTCAAAGAGAGGAATTACAAGTAAA
CTTCAAAGTTATAAGATTCTCATGTGGGAAGGTTATGGAGCAACAGTGAAAGTAACAACA
GCTGATATCACAACTGAGCAAGGATGTGAACAAGCTCTTAATGAAGCATCACAACTTGGA
CCAGTTACAGCCATTTTCAATCTTGCAGTTGCACTTCGTGATGGAATTTTCGATAATTTA
GATTTGAAAATGTTTGAAGAATCTTTGGCACCTAAAGCAGCAGCAACAAAAAATCTTGAT
CAAATTTCACGCAAATTGTGTCCAAATTTGAAGCATTTTGTTGTGTTTTCAAGTTTCTCT
TGTGGTCGTGGCAATGCAGGTCAAAGTAATTATGGCTTAGGAAATTCAATTATGGAAAGA
ATTATTGAAGAAAGACGAGAACTTGGTTTGCCAGCAAAAGCAATTCAATGGGCAGCAGTC
GGTGATGTTGGTTTGCTTGCAGATTTTCAATTGAATAATTTAGATAAAGATTTTAAAGGA
ACCTTACCACAAGCGATTTCTTCATGTCTCGAAGTGCTTGATGTTTTAATGACATCAGAT
GATGCAATAGTTTCAAGTATGGTTGTTGCTGATAAGACAATGAATGATGTAAAGAAAGGC
AACATTATAGATATGATCTTAAAAATCATGGGAATTCGTGATAGGAAATCAATCTCAATG
GATTCGACACTTACACAACTTGGCATCGACTCTTTGATGGGCGTAGAAATTCAGCAGATT
TTAGAAAGAGATTTTGATATTGCATTGACATCACAAGAGTTGCGATCACTCACATTAAGT
CAATTAGAAAAGCGTGCAATTTCAAAAGGTGAAAATAGTGACAGTAATGTTGTCAATGAA
TTACCATGGACTAGTCTTTTACTTGATGGAGCAATTAATCCAGAAACAAGAGAATTACAT
ACAAGTGATACAATTGTTAAAATTAATGATATTGAGACAAATAATAGTACAAAAATTTTG
ATTCTTCCTGGCTTTTATGGAGTTGCAGCTGATATTTATAGAAATATTGCCAAACAAATG
AATCAACCTGCCTTTGTCATGCAATATATTGAATCATCAGAATGTACAGCAATAGAAGAA
ATAGTTGATATAATTAAGCCACAAATTCTTAATCTCTTTGATGATTGTAAGAATTTCGTA
CTGATCGCTCATTCATTCAGTGTCATCACATCTCTTAATATTGCACAATCATTAGAAAAA
ATTGGAAAGAATGGAAAAGTCATTGTAATTGATAGTTCCATACAATTTTGTAAAAAATAT
GCTCTTAAAAGAACAACTGAAAACAAAGATGAAAAAATCAAAACTAATCTATCAAGAATT
TTATTTGAGAGTTTCATGACTTATGCTGATCCTGAAGTTGCAAAGAAAGCATTTTATAAG
TATGAACAATGGGAAGATAAACTGGATGAACTGTTAAGAAATTCAACTGATAGAATTCCA
TATACATTTGAATTCATTAAACGAAATGTTTTGACAGCATTTGTTAATAGATTCAATATG
GCATTAAATTTAATGGAAGAATCATTTAATACTCTAAATTCAACAAAACTCGCTTTAATT
AAAGCGTCAAAACCTTCAGTTGAAGGAATGCCAAGAGATTATGGATTATCAAGACATTCT
AAAGATCCTATCAATGTACAAGTACTTGAAGGTGATCATGTGACTATAACATTAAATCCG
GAACTACCGAATGTAATTAAAAGTATCATAGAATAA
>g13476.t1 Gene=g13476 Length=1511
MEFFIESLGKLFQQGVDMDISKLYPPIQFPVSRNTPMISPNIKWNHTENHFVPYYDSFNW
YERRNIIININDKVFEPVQGHLIDGRCLFPATGWIFLVWDTFASMNGMPVDKARVVFEDI
QFLRATALNKNQDVLITISIHRGTGRFEVIEGVSAVVHGYIKHDDEIEMSEFTVPKTDNP
LKLETDDFYKEMRLSGFCHLNKLQGIKEIRHDGLEGKIKWEDDWIAFIDSMTHFEEFETK
SKILALPTNIRKIIIDPILHLKMLEEKKKELEGVEDDKKEILYDAKISPYLRIIQAGGVE
IHEKKNRVVNRRRPKEPTLESQKFIPFFANEKFSLKDAAKIITQTALETIFQPDFAALKL
ILILSSFNGVDLINKSNCVNDNEFLHSIKSVLNSNGYILSREQSTASEIDEKLQLVAKVS
LENGEVLHMLQFKDNIKLFEPQNIIEITSNVSEWLEPLKSALKEGTTLVYSYNHDYSGIL
GLVNCVRREYPPERLKCIFLIDTKTAPAIFDINDEFFKSQLLLNHTINVYKNQLWGGYRF
LDITEETDKKPQKNHCFANCIIRGDLSSLSWLKGPFDVHDSANKDLIRIQYSSLNFKDVM
LALGRILYKEFDRLERQCVLGLEFSGLKADNTRVMGIALKAGALATHYDSKYSILWNVPD
NWSLEEAATVPLVYFTVYFAFFVTTKIKSGKSILIHSGTGGVGQAAIQVAFAYGLEVFTT
VGSDDKKEFFTLKKFPKLKPENIGNSRNTTFERMILENTKGNGVDYVLNSLADDKLKASI
RCLAKDGTLLEIGKYDMLKGTKLDMRYLTKRIKLKAIHFDDLPTDSDEMKAVYSHIDKDL
KSGIIQPLNTTVFDANDVEEAFRYMASGKHMGKVLLKVRQNEHDESSLPLMALDRVFVDA
NETVFIAGGLGGFGMELADWLILRGCRKVMLSSKRGITSKLQSYKILMWEGYGATVKVTT
ADITTEQGCEQALNEASQLGPVTAIFNLAVALRDGIFDNLDLKMFEESLAPKAAATKNLD
QISRKLCPNLKHFVVFSSFSCGRGNAGQSNYGLGNSIMERIIEERRELGLPAKAIQWAAV
GDVGLLADFQLNNLDKDFKGTLPQAISSCLEVLDVLMTSDDAIVSSMVVADKTMNDVKKG
NIIDMILKIMGIRDRKSISMDSTLTQLGIDSLMGVEIQQILERDFDIALTSQELRSLTLS
QLEKRAISKGENSDSNVVNELPWTSLLLDGAINPETRELHTSDTIVKINDIETNNSTKIL
ILPGFYGVAADIYRNIAKQMNQPAFVMQYIESSECTAIEEIVDIIKPQILNLFDDCKNFV
LIAHSFSVITSLNIAQSLEKIGKNGKVIVIDSSIQFCKKYALKRTTENKDEKIKTNLSRI
LFESFMTYADPEVAKKAFYKYEQWEDKLDELLRNSTDRIPYTFEFIKRNVLTAFVNRFNM
ALNLMEESFNTLNSTKLALIKASKPSVEGMPRDYGLSRHSKDPINVQVLEGDHVTITLNP
ELPNVIKSIIE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 21 | g13476.t1 | CDD | cd05195 | enoyl_red | 587 | 876 | 2.77045E-94 |
| 20 | g13476.t1 | CDD | cd08954 | KR_1_FAS_SDR_x | 903 | 1130 | 1.25539E-59 |
| 19 | g13476.t1 | Coils | Coil | Coil | 261 | 284 | - |
| 18 | g13476.t1 | Coils | Coil | Coil | 1394 | 1414 | - |
| 12 | g13476.t1 | Gene3D | G3DSA:3.30.70.3290 | - | 7 | 63 | 2.0E-14 |
| 13 | g13476.t1 | Gene3D | G3DSA:3.10.129.110 | - | 67 | 315 | 5.6E-57 |
| 11 | g13476.t1 | Gene3D | G3DSA:3.40.50.720 | - | 452 | 1129 | 2.1E-254 |
| 15 | g13476.t1 | Gene3D | G3DSA:3.90.180.10 | - | 545 | 896 | 2.1E-254 |
| 14 | g13476.t1 | Gene3D | G3DSA:1.10.1200.10 | - | 1133 | 1219 | 2.1E-17 |
| 17 | g13476.t1 | Gene3D | G3DSA:3.40.50.1820 | - | 1258 | 1501 | 3.1E-26 |
| 16 | g13476.t1 | Gene3D | G3DSA:1.10.1470.20 | Fatty acid synthase; domain 2 | 1357 | 1448 | 3.1E-26 |
| 4 | g13476.t1 | PANTHER | PTHR43775 | FATTY ACID SYNTHASE | 2 | 1510 | 0.0 |
| 5 | g13476.t1 | PANTHER | PTHR43775:SF23 | FATTY ACID SYNTHASE 3 | 2 | 1510 | 0.0 |
| 2 | g13476.t1 | Pfam | PF00107 | Zinc-binding dehydrogenase | 701 | 825 | 4.2E-15 |
| 3 | g13476.t1 | Pfam | PF08659 | KR domain | 903 | 1081 | 1.6E-39 |
| 1 | g13476.t1 | Pfam | PF00550 | Phosphopantetheine attachment site | 1142 | 1202 | 2.1E-7 |
| 25 | g13476.t1 | ProSiteProfiles | PS50075 | Carrier protein (CP) domain profile. | 1133 | 1213 | 10.05 |
| 22 | g13476.t1 | SMART | SM00829 | PKS_ER_names_mod | 564 | 876 | 5.7E-100 |
| 24 | g13476.t1 | SMART | SM00822 | This enzymatic domain is part of bacterial polyketide synthases and catalyses the first step in the reductive modification of the beta-carbonyl centres in the growing polyketide chain. It uses NADPH to reduce the keto group to a hydroxy group. | 902 | 1083 | 3.0E-19 |
| 23 | g13476.t1 | SMART | SM00823 | Phosphopantetheine attachment site | 1139 | 1197 | 7.6E-4 |
| 9 | g13476.t1 | SUPERFAMILY | SSF50129 | GroES-like | 582 | 688 | 7.54E-14 |
| 8 | g13476.t1 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 661 | 824 | 1.89E-34 |
| 7 | g13476.t1 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 900 | 1118 | 1.1E-27 |
| 10 | g13476.t1 | SUPERFAMILY | SSF47336 | ACP-like | 1142 | 1203 | 2.36E-9 |
| 6 | g13476.t1 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 1240 | 1507 | 3.75E-14 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0031177 | phosphopantetheine binding | MF |
| GO:0055114 | NA | NA |
| GO:0016491 | oxidoreductase activity | MF |
| GO:0004312 | fatty acid synthase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
