Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Cytochrome P450 9e2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g13580 g13580.t29 isoform g13580.t29 31042311 31042954
chr_1 g13580 g13580.t29 exon g13580.t29.exon1 31042311 31042714
chr_1 g13580 g13580.t29 cds g13580.t29.CDS1 31042324 31042714
chr_1 g13580 g13580.t29 exon g13580.t29.exon2 31042767 31042954
chr_1 g13580 g13580.t29 cds g13580.t29.CDS2 31042767 31042954
chr_1 g13580 g13580.t29 TTS g13580.t29 31043048 31043048
chr_1 g13580 g13580.t29 TSS g13580.t29 NA NA

Sequences

>g13580.t29 Gene=g13580 Length=592
AGTTTCAAATGTGATGACCTTTATGGCATATGAACTCATTCTCAATCCTGATATTCAAAA
GAAATTGCAAGATGAAATTGATGCAATGAATGCTGAACTCAAAGGAGGTGATTTGACTTA
TGAAGATGTTCAGAAACTAAAATATATGGATATGGTACTTTGTGAAACTTTGAGAATGTG
GCCACCAGCTCCTATCATTGATAGAATGTGTACAAAAGATTTTCTCTTGGAATATGACAA
CAAAAAAGTTCAAATTGAAGTTGGCAGAAATTTCTATATTCCTGTCTATTCTTTGCATCA
TAACGAAAACTACTTTCCAAATCCGAATAGATTTGATCCAGAACGTTTCAGTGATGAAAA
CAAGAAAAACATCAGACAGGATTGTTATTTACCTTTTGGAATTGGTCCAAGGAATTGCAT
CGGAAATCGTTTCGCTTTACTTGAAGTCAAGACAATCTTCTATTATCTTCTTTTGAATTT
TAATTTTGAAGCAACAAAGGACACACAAATTCCAATTAAATTGGCAAACAATCAAGCTAC
TTTCCAATTCGAGAAGGGCTTGCATTGCGCATTAGTTCCAAGAACAAAATAA

>g13580.t29 Gene=g13580 Length=192
MTFMAYELILNPDIQKKLQDEIDAMNAELKGGDLTYEDVQKLKYMDMVLCETLRMWPPAP
IIDRMCTKDFLLEYDNKKVQIEVGRNFYIPVYSLHHNENYFPNPNRFDPERFSDENKKNI
RQDCYLPFGIGPRNCIGNRFALLEVKTIFYYLLLNFNFEATKDTQIPIKLANNQATFQFE
KGLHCALVPRTK

Protein features from InterProScan

Transcript Database ID Name Start End E.value
14 g13580.t29 Gene3D G3DSA:1.10.630.10 Cytochrome p450 1 192 1.6E-67
2 g13580.t29 PANTHER PTHR24292 CYTOCHROME P450 1 175 1.6E-82
3 g13580.t29 PANTHER PTHR24292:SF90 CYTOCHROME P450 317A1-RELATED 1 175 1.6E-82
7 g13580.t29 PRINTS PR00463 E-class P450 group I signature 1 23 8.1E-20
9 g13580.t29 PRINTS PR00385 P450 superfamily signature 10 23 9.1E-13
11 g13580.t29 PRINTS PR00385 P450 superfamily signature 47 58 9.1E-13
5 g13580.t29 PRINTS PR00463 E-class P450 group I signature 90 114 8.1E-20
4 g13580.t29 PRINTS PR00463 E-class P450 group I signature 125 135 8.1E-20
10 g13580.t29 PRINTS PR00385 P450 superfamily signature 126 135 9.1E-13
6 g13580.t29 PRINTS PR00463 E-class P450 group I signature 135 158 8.1E-20
8 g13580.t29 PRINTS PR00385 P450 superfamily signature 135 146 9.1E-13
1 g13580.t29 Pfam PF00067 Cytochrome P450 2 176 5.3E-52
13 g13580.t29 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 128 137 -
12 g13580.t29 SUPERFAMILY SSF48264 Cytochrome P450 1 190 3.67E-63

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values