Gene loci information

Transcript annotation

  • This transcript has been annotated as 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g13583 g13583.t11 TTS g13583.t11 31050505 31050505
chr_1 g13583 g13583.t11 isoform g13583.t11 31050622 31053399
chr_1 g13583 g13583.t11 exon g13583.t11.exon1 31050622 31050675
chr_1 g13583 g13583.t11 exon g13583.t11.exon2 31051107 31051525
chr_1 g13583 g13583.t11 cds g13583.t11.CDS1 31051262 31051525
chr_1 g13583 g13583.t11 exon g13583.t11.exon3 31051578 31051796
chr_1 g13583 g13583.t11 cds g13583.t11.CDS2 31051578 31051796
chr_1 g13583 g13583.t11 exon g13583.t11.exon4 31051852 31052244
chr_1 g13583 g13583.t11 cds g13583.t11.CDS3 31051852 31052244
chr_1 g13583 g13583.t11 exon g13583.t11.exon5 31052324 31052487
chr_1 g13583 g13583.t11 cds g13583.t11.CDS4 31052324 31052487
chr_1 g13583 g13583.t11 exon g13583.t11.exon6 31052551 31052676
chr_1 g13583 g13583.t11 cds g13583.t11.CDS5 31052551 31052676
chr_1 g13583 g13583.t11 exon g13583.t11.exon7 31053345 31053399
chr_1 g13583 g13583.t11 cds g13583.t11.CDS6 31053345 31053399
chr_1 g13583 g13583.t11 TSS g13583.t11 31053880 31053880

Sequences

>g13583.t11 Gene=g13583 Length=1430
ATGGACATGCAGCTAGGTTGGAAATTTTTACACAAAGAAACATTCATTCATACAGGTGAT
ACTACGAATTTCATAAATGTGCCCCACGTGATTGCTATGGTTGGCCTACCTGCTCGAGGA
AAGACATATATTGCAAAAAAGTTGTCGAGATATTTAAATTGGATCGGTATCAACACTAGA
GTCTTTAATTTAGGCGAGTATCGTCGTAACGTAACACAAGCATATAGAAATCATGATTTC
TTTCGACCTGATAATGACATAGCTATGCAGATTAGAACAAGTTGTGCAAAGCACGCGCTA
GATGACGTTGTGCAATGGCTTGAAACGGGAGGAGGAGAAGTTGCAGTTTTTGATGCAACA
AACTCGACATACGAACGACGCAAAATGATTGAAGAAGTTGTTGTTAAACAAAAAAAATTT
AAGCTTCTTTTCGTTGAGTCAATTTGCGATGATCCGAATATCATTGAACAGAACATTATG
GAAGTAAAAGTTAGTAGTCCTGATTATGCAAATATGAACAAAGACGATGCAATGAACGAT
TTTCGGCAAAGGATTGAGCATTATCAAGAAAAATATCAGCCTCTAGATCGAGAACAGGAG
AAAGACTATTCTTATATGAAAATTTACAACACCGGTGAAAAGATAATAATTCACAAACAC
GAAGGTCACATTCAATCACGAATTGTGTACTTTTTAATGAATATACACATCACAAAGAGA
ACAATTTATTTGACTCGACATGGTGAAAGTGAACACAACTTGAAGGGTTTGATTGGAGGT
GATTCTGACTTGAGTGTTCGTGGAAAATTATACGCAGAAGCTCTTGCCAATTATATCAAC
GATCAAAACATTGAAGGATTGCGTGTATGGACATCATGGCTTAAAAGAACGATTCAGACA
GTTGCAGAAATAAAAGCACCTCAAGAGAGATGGAAGGCTCTTAATGAGATTGATGCTGGT
ATTTGCGAGGAAATGACTTATGAAGAGATTCAAAAGAAATTCCCCGAAGATTTCCGTGCT
CGTGACCAAAATAAATTCTTTTATCGTTACCCACGCGGTGAAAGCTATGAAGATTTAGTT
GTGAGATTAGAACCAGTAATGATGGAATTAGAGCGACAAGGTAATGTACTTGTTGTTACT
CATCAAGCCGTACTTCGATGCATTTTGGCTTACTTTTTGGATAAGCCAGCGAGTGAGTGT
TATAAAGAAAAGAAAATATAAAATGATACTAAAAACGTTTATTTGTAGGCGAACTGCCAT
ATCTTGAAGTTCCATTACATACAATTATTAAATTGACACCTGTGGCATATGGATGTAAAT
GTGAATTTATTAAAATTCCTGTTGATGCAGTTAGTACTCATCGCCCAAAACCAGAGGTTC
CTGGAACAATTGAAAAACTTATAAATAGATCGAATGGTGAGATTGAATGA

>g13583.t11 Gene=g13583 Length=406
MDMQLGWKFLHKETFIHTGDTTNFINVPHVIAMVGLPARGKTYIAKKLSRYLNWIGINTR
VFNLGEYRRNVTQAYRNHDFFRPDNDIAMQIRTSCAKHALDDVVQWLETGGGEVAVFDAT
NSTYERRKMIEEVVVKQKKFKLLFVESICDDPNIIEQNIMEVKVSSPDYANMNKDDAMND
FRQRIEHYQEKYQPLDREQEKDYSYMKIYNTGEKIIIHKHEGHIQSRIVYFLMNIHITKR
TIYLTRHGESEHNLKGLIGGDSDLSVRGKLYAEALANYINDQNIEGLRVWTSWLKRTIQT
VAEIKAPQERWKALNEIDAGICEEMTYEEIQKKFPEDFRARDQNKFFYRYPRGESYEDLV
VRLEPVMMELERQGNVLVVTHQAVLRCILAYFLDKPASECYKEKKI

Protein features from InterProScan

Transcript Database ID Name Start End E.value
16 g13583.t11 CDD cd07067 HP_PGM_like 241 402 1.2192E-28
14 g13583.t11 Coils Coil Coil 178 198 -
12 g13583.t11 Gene3D G3DSA:3.40.50.300 - 27 237 6.2E-91
13 g13583.t11 Gene3D G3DSA:3.40.50.1240 - 238 405 8.7E-65
3 g13583.t11 PANTHER PTHR10606 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE 18 400 3.0E-181
15 g13583.t11 PIRSF PIRSF000709 6PFK_fruc_bisph_Ptase 10 402 2.0E-190
6 g13583.t11 PRINTS PR00991 6-phosphofructo-2-kinase family signature 114 128 3.2E-47
7 g13583.t11 PRINTS PR00991 6-phosphofructo-2-kinase family signature 141 155 3.2E-47
4 g13583.t11 PRINTS PR00991 6-phosphofructo-2-kinase family signature 167 181 3.2E-47
9 g13583.t11 PRINTS PR00991 6-phosphofructo-2-kinase family signature 220 241 3.2E-47
8 g13583.t11 PRINTS PR00991 6-phosphofructo-2-kinase family signature 242 264 3.2E-47
5 g13583.t11 PRINTS PR00991 6-phosphofructo-2-kinase family signature 319 335 3.2E-47
1 g13583.t11 Pfam PF01591 6-phosphofructo-2-kinase 22 238 1.9E-87
2 g13583.t11 Pfam PF00300 Histidine phosphatase superfamily (branch 1) 242 400 1.0E-37
18 g13583.t11 ProSitePatterns PS00175 Phosphoglycerate mutase family phosphohistidine signature. 244 253 -
17 g13583.t11 SMART SM00855 PGAM_5 241 388 1.9E-19
11 g13583.t11 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases 28 239 8.99E-42
10 g13583.t11 SUPERFAMILY SSF53254 Phosphoglycerate mutase-like 241 400 1.39E-53

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006003 fructose 2,6-bisphosphate metabolic process BP
GO:0005524 ATP binding MF
GO:0006000 fructose metabolic process BP
GO:0003824 catalytic activity MF
GO:0003873 6-phosphofructo-2-kinase activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values