Gene loci information

Transcript annotation

  • This transcript has been annotated as 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g13583 g13583.t9 TTS g13583.t9 31050505 31050505
chr_1 g13583 g13583.t9 isoform g13583.t9 31050622 31052486
chr_1 g13583 g13583.t9 exon g13583.t9.exon1 31050622 31050675
chr_1 g13583 g13583.t9 cds g13583.t9.CDS1 31050622 31050675
chr_1 g13583 g13583.t9 exon g13583.t9.exon2 31051107 31051234
chr_1 g13583 g13583.t9 cds g13583.t9.CDS2 31051107 31051234
chr_1 g13583 g13583.t9 exon g13583.t9.exon3 31051291 31051525
chr_1 g13583 g13583.t9 cds g13583.t9.CDS3 31051291 31051525
chr_1 g13583 g13583.t9 exon g13583.t9.exon4 31051578 31051796
chr_1 g13583 g13583.t9 cds g13583.t9.CDS4 31051578 31051796
chr_1 g13583 g13583.t9 exon g13583.t9.exon5 31051852 31052244
chr_1 g13583 g13583.t9 cds g13583.t9.CDS5 31051852 31052244
chr_1 g13583 g13583.t9 exon g13583.t9.exon6 31052324 31052486
chr_1 g13583 g13583.t9 cds g13583.t9.CDS6 31052324 31052404
chr_1 g13583 g13583.t9 TSS g13583.t9 NA NA

Sequences

>g13583.t9 Gene=g13583 Length=1192
CTTTAATTTAGGCGAGTATCGTCGTAACGTAACACAAGCATATAGAAATCATGATTTCTT
TCGACCTGATAATGACATAGCTATGCAGATTAGAACAAGTTGTGCAAAGCACGCGCTAGA
TGACGTTGTGCAATGGCTTGAAACGGGAGGAGGAGAAGTTGCAGTTTTTGATGCAACAAA
CTCGACATACGAACGACGCAAAATGATTGAAGAAGTTGTTGTTAAACAAAAAAAATTTAA
GCTTCTTTTCGTTGAGTCAATTTGCGATGATCCGAATATCATTGAACAGAACATTATGGA
AGTAAAAGTTAGTAGTCCTGATTATGCAAATATGAACAAAGACGATGCAATGAACGATTT
TCGGCAAAGGATTGAGCATTATCAAGAAAAATATCAGCCTCTAGATCGAGAACAGGAGAA
AGACTATTCTTATATGAAAATTTACAACACCGGTGAAAAGATAATAATTCACAAACACGA
AGGTCACATTCAATCACGAATTGTGTACTTTTTAATGAATATACACATCACAAAGAGAAC
AATTTATTTGACTCGACATGGTGAAAGTGAACACAACTTGAAGGGTTTGATTGGAGGTGA
TTCTGACTTGAGTGTTCGTGGAAAATTATACGCAGAAGCTCTTGCCAATTATATCAACGA
TCAAAACATTGAAGGATTGCGTGTATGGACATCATGGCTTAAAAGAACGATTCAGACAGT
TGCAGAAATAAAAGCACCTCAAGAGAGATGGAAGGCTCTTAATGAGATTGATGCTGGTAT
TTGCGAGGAAATGACTTATGAAGAGATTCAAAAGAAATTCCCCGAAGATTTCCGTGCTCG
TGACCAAAATAAATTCTTTTATCGTTACCCACGCGGTGAAAGCTATGAAGATTTAGTTGT
GAGATTAGAACCAGTAATGATGGAATTAGAGCGACAAGGTAATGTACTTGTTGTTACTCA
TCAAGCCGTACTTCGATGCATTTTGGCTTACTTTTTGGATAAGCCAGCGAGCGAACTGCC
ATATCTTGAAGTTCCATTACATACAATTATTAAATTGACACCTGTGGCATATGGATGTAA
ATGTGAATTTATTAAAATTCCTGTTGATGCAGTTAGTACTCATCGCCCAAAACCAGAGGT
TCCTGGAACAATTGAAAAACTTATAAATAGATCGAATGGTGAGATTGAATGA

>g13583.t9 Gene=g13583 Length=369
MQIRTSCAKHALDDVVQWLETGGGEVAVFDATNSTYERRKMIEEVVVKQKKFKLLFVESI
CDDPNIIEQNIMEVKVSSPDYANMNKDDAMNDFRQRIEHYQEKYQPLDREQEKDYSYMKI
YNTGEKIIIHKHEGHIQSRIVYFLMNIHITKRTIYLTRHGESEHNLKGLIGGDSDLSVRG
KLYAEALANYINDQNIEGLRVWTSWLKRTIQTVAEIKAPQERWKALNEIDAGICEEMTYE
EIQKKFPEDFRARDQNKFFYRYPRGESYEDLVVRLEPVMMELERQGNVLVVTHQAVLRCI
LAYFLDKPASELPYLEVPLHTIIKLTPVAYGCKCEFIKIPVDAVSTHRPKPEVPGTIEKL
INRSNGEIE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
17 g13583.t9 CDD cd07067 HP_PGM_like 153 331 3.21046E-33
15 g13583.t9 Coils Coil Coil 90 110 -
13 g13583.t9 Gene3D G3DSA:3.40.50.300 - 1 149 2.6E-57
14 g13583.t9 Gene3D G3DSA:3.40.50.1240 - 150 362 1.8E-78
3 g13583.t9 PANTHER PTHR10606:SF44 6-PHOSPHOFRUCTO 2-KINASE/FRUCTOSE 2,6-BISPHOSPHATASE LONG FORM 1 350 5.5E-166
4 g13583.t9 PANTHER PTHR10606 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE 1 350 5.5E-166
16 g13583.t9 PIRSF PIRSF000709 6PFK_fruc_bisph_Ptase 1 366 5.1E-174
7 g13583.t9 PRINTS PR00991 6-phosphofructo-2-kinase family signature 26 40 1.9E-47
9 g13583.t9 PRINTS PR00991 6-phosphofructo-2-kinase family signature 53 67 1.9E-47
5 g13583.t9 PRINTS PR00991 6-phosphofructo-2-kinase family signature 79 93 1.9E-47
6 g13583.t9 PRINTS PR00991 6-phosphofructo-2-kinase family signature 132 153 1.9E-47
10 g13583.t9 PRINTS PR00991 6-phosphofructo-2-kinase family signature 154 176 1.9E-47
8 g13583.t9 PRINTS PR00991 6-phosphofructo-2-kinase family signature 231 247 1.9E-47
1 g13583.t9 Pfam PF01591 6-phosphofructo-2-kinase 1 150 4.3E-55
2 g13583.t9 Pfam PF00300 Histidine phosphatase superfamily (branch 1) 154 335 3.5E-40
19 g13583.t9 ProSitePatterns PS00175 Phosphoglycerate mutase family phosphohistidine signature. 156 165 -
18 g13583.t9 SMART SM00855 PGAM_5 153 300 1.9E-19
12 g13583.t9 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases 7 151 1.28E-20
11 g13583.t9 SUPERFAMILY SSF53254 Phosphoglycerate mutase-like 153 342 1.12E-58

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006003 fructose 2,6-bisphosphate metabolic process BP
GO:0005524 ATP binding MF
GO:0006000 fructose metabolic process BP
GO:0003824 catalytic activity MF
GO:0003873 6-phosphofructo-2-kinase activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values