General information for g13719.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as SUMO-activating enzyme subunit 2.
Parent gene
- The parent gene g13719 is located on chr_1: 32030356..32030580 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g13719 | g13719.t1 | TTS | g13719.t1 | 32028155 | 32028155 |
| chr_1 | g13719 | g13719.t1 | isoform | g13719.t1 | 32028476 | 32030566 |
| chr_1 | g13719 | g13719.t1 | exon | g13719.t1.exon1 | 32028476 | 32029146 |
| chr_1 | g13719 | g13719.t1 | cds | g13719.t1.CDS1 | 32028476 | 32029146 |
| chr_1 | g13719 | g13719.t1 | exon | g13719.t1.exon2 | 32029210 | 32030356 |
| chr_1 | g13719 | g13719.t1 | cds | g13719.t1.CDS2 | 32029210 | 32030356 |
| chr_1 | g13719 | g13719.t1 | exon | g13719.t1.exon3 | 32030423 | 32030566 |
| chr_1 | g13719 | g13719.t1 | cds | g13719.t1.CDS3 | 32030423 | 32030566 |
| chr_1 | g13719 | g13719.t1 | TSS | g13719.t1 | 32030742 | 32030742 |
Sequences
>g13719.t1 Gene=g13719 Length=1962
ATGGCTGCAGAAATTGATGGATTCTTTCGAGACGATTTACGAGAAAAAATTCTCACTTCT
AAATTACTCGTAGTTGGTGCCGGCGGTATTGGTTGTGAAGTGCTCAAAAACCTTGTTATG
TGCGGCTTTAAAAATATTGAAATTATTGATCTTGACACAATTGATGTATCGAATCTCAAC
AGACAATTTCTATTTCACAAAGAACATGTTGGAAAGTCAAAAGCTATGGTTGCTCGTGAA
ACTGCACTCAAATTTAATCCGAATGTAGAGATTAAGGCTTATCATGACAGTATTTTTAAT
ACAGATTATGGTGTAAGCTTCTTTCAACGTTTTACTATGGTTTTAAATGCATTGGACAAT
CGTGCTGCACGTAATCATGTTAATCGAATGTGTTTGAATGCAAAAATTCCATTGATTGAA
AGTGGCACTGCTGGATATAATGGTCAAGTTGAATTGATTGAACGTGGTGTTACTCAATGT
TATGAATGTACTCCAAAAGCAGCTCAAAAAACATTCCCCGGTTGCACAATTCGAAACACA
CCATCAGAACCAGTTCATTGCATTGTTTGGGCTAAACATCTCTTTAATCAATTATTTGGT
GAAGACAATGCAGATGAAGATGTCTCACCTGATACTGCAGATCCAGAAGCCGCAGGTGAT
GCTGGAAAAGATGCTATGGAAGTTGATTCAAATGAGAAAGGAAATATTGAGAGAGTAAGC
ACAAAACAGTGGGCGCAAAATTGTGATTACATGCCTGAGAAATTGTTCAACAAGTTTTTC
TCGGATGATATCAATTATCTTTTGAGCATGTCAAATTTATGGAAAACACGTACACCTCCC
AAACCAATGAGATATGGTGAACACGATGTTATTTCGGAAGTAGAAGACGTTTCTTCAACA
ACTGGTGTTATGCGAGATCAAAAGATATGGACTGTTGGTGAATGTCAAAAGGTGTTTGCT
GAAAGTATCTCTAATCTCAAAGAGCAAGCCTCTAAATTAAAGGAAGATGATCATTTAGTT
TGGGACAAGGATGATAAAGATGCAATGGATTTTGTAGCAGCATGTGCTAACATTCGCTCA
ATGATTTTCTCAATTCCTCAAAAGTCTCGATTTGATATTAAATCAATGGCTGGTAACATT
ATTCCTGCGATTGCCACAACTAATGCAATTACTTCTGGTTTTGTTGTTCTTCATGCTTTC
AAAGTGTTGGAAAAGAAGTTTGAGCAGTGTCAGAGTGTATATATTCGATCACAAGTAAAT
GCACGCAATCAAATTTTTGTACCGGAAAAAATTATCACACCGCCAAATCCAAATTGCTAC
GTTTGTGCCGCTAAACCTGAGCTCATTTTGAAGGTCGATACAGATGTAATTACAGTTAGA
GAATTTCGTGACGATATTTTGATTAAGGAAATGAATATGGTTAATCCGGATGTTATGATT
GATGGCAAAGGAATCATTGTTATTTCATCTGAAGAGGGCGAGACTGAATGTAATGAAGAA
AAGTTGCTTAAAGAAATGCAAATTGTCGATGGATGTATCTTAAAGGCCGAAGATTTCTTT
CAAAATTATGAGCTCACAATTACAATCGTACATAAGAAAGCAGAACGTGAAGATGCAAAG
TTTGAAATTATTGCCGATAAAAATATTTTAAAAACTGCTGAAAGTGTTGAAGAGTTAAAG
AAACAAAAAACTGCCGAAGCTGCTTCTCAATCTCAAGAACCTGGACCTTCTGGCTTAAAT
GGATCATCTTCAAATGGAGGTGAAAGCAGCAAGAATGTTGCATATGAGAGCGATGAAGAT
GATTTAGTTCTTGTTGAAGAAACTCCAGCAAGTGCAGAGAAACGAAAGCACATTGATGAA
GATGAATCACCATCATGCAAACGAGTAAAAATTGATGAAAGTGAGAAACCAGCAACTCTA
AATGATGAAGATGATGACTTGATTTGCATCGATGATGATTAA
>g13719.t1 Gene=g13719 Length=653
MAAEIDGFFRDDLREKILTSKLLVVGAGGIGCEVLKNLVMCGFKNIEIIDLDTIDVSNLN
RQFLFHKEHVGKSKAMVARETALKFNPNVEIKAYHDSIFNTDYGVSFFQRFTMVLNALDN
RAARNHVNRMCLNAKIPLIESGTAGYNGQVELIERGVTQCYECTPKAAQKTFPGCTIRNT
PSEPVHCIVWAKHLFNQLFGEDNADEDVSPDTADPEAAGDAGKDAMEVDSNEKGNIERVS
TKQWAQNCDYMPEKLFNKFFSDDINYLLSMSNLWKTRTPPKPMRYGEHDVISEVEDVSST
TGVMRDQKIWTVGECQKVFAESISNLKEQASKLKEDDHLVWDKDDKDAMDFVAACANIRS
MIFSIPQKSRFDIKSMAGNIIPAIATTNAITSGFVVLHAFKVLEKKFEQCQSVYIRSQVN
ARNQIFVPEKIITPPNPNCYVCAAKPELILKVDTDVITVREFRDDILIKEMNMVNPDVMI
DGKGIIVISSEEGETECNEEKLLKEMQIVDGCILKAEDFFQNYELTITIVHKKAEREDAK
FEIIADKNILKTAESVEELKKQKTAEAASQSQEPGPSGLNGSSSNGGESSKNVAYESDED
DLVLVEETPASAEKRKHIDEDESPSCKRVKIDESEKPATLNDEDDDLICIDDD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g13719.t1 | CDD | cd01489 | Uba2_SUMO | 21 | 442 | 0.0 |
| 10 | g13719.t1 | Coils | Coil | Coil | 553 | 573 | - |
| 7 | g13719.t1 | Gene3D | G3DSA:3.40.50.720 | - | 20 | 442 | 7.4E-181 |
| 8 | g13719.t1 | Gene3D | G3DSA:1.10.10.520 | Ubiquitin activating enzymes (Uba3). Chain: B | 170 | 379 | 7.4E-181 |
| 9 | g13719.t1 | Gene3D | G3DSA:3.10.290.20 | - | 443 | 573 | 1.1E-39 |
| 18 | g13719.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 206 | 233 | - |
| 14 | g13719.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 217 | 233 | - |
| 17 | g13719.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 561 | 653 | - |
| 16 | g13719.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 565 | 592 | - |
| 15 | g13719.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 607 | 643 | - |
| 4 | g13719.t1 | PANTHER | PTHR10953 | UBIQUITIN-ACTIVATING ENZYME E1 | 18 | 519 | 4.0E-165 |
| 5 | g13719.t1 | PANTHER | PTHR10953:SF5 | SUMO-ACTIVATING ENZYME SUBUNIT 2 | 18 | 519 | 4.0E-165 |
| 11 | g13719.t1 | PIRSF | PIRSF039133 | SUMO_E1B | 1 | 645 | 1.9E-198 |
| 2 | g13719.t1 | Pfam | PF00899 | ThiF family | 9 | 440 | 1.5E-71 |
| 3 | g13719.t1 | Pfam | PF10585 | Ubiquitin-activating enzyme active site | 320 | 374 | 2.1E-5 |
| 1 | g13719.t1 | Pfam | PF14732 | Ubiquitin/SUMO-activating enzyme ubiquitin-like domain | 450 | 534 | 1.2E-20 |
| 13 | g13719.t1 | ProSitePatterns | PS00865 | Ubiquitin-activating enzyme active site. | 173 | 181 | - |
| 6 | g13719.t1 | SUPERFAMILY | SSF69572 | Activating enzymes of the ubiquitin-like proteins | 9 | 469 | 3.79E-100 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008641 | ubiquitin-like modifier activating enzyme activity | MF |
| GO:0016925 | protein sumoylation | BP |
| GO:0019948 | SUMO activating enzyme activity | MF |
KEGG
Orthology
- This transcript has been annotated with K10685 (ubiquitin-like 1-activating enzyme E1 B)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
