General information for g13914.t5

Gene loci information

Transcript annotation

This transcript has been annotated as Major facilitator superfamily domain-containing protein 8.

Parent gene

The parent gene g13914 is located on chr_1: 33373984..33375461 (+ strand)

Gene structure

The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome	Gene	Transcript	Category	ID	Start	End
chr_1	g13914	g13914.t5	isoform	g13914.t5	33373157	33375461
chr_1	g13914	g13914.t5	TSS	g13914.t5	33373157	33373157
chr_1	g13914	g13914.t5	exon	g13914.t5.exon1	33373157	33373312
chr_1	g13914	g13914.t5	exon	g13914.t5.exon2	33373384	33373565
chr_1	g13914	g13914.t5	exon	g13914.t5.exon3	33373643	33373905
chr_1	g13914	g13914.t5	cds	g13914.t5.CDS1	33373717	33373905
chr_1	g13914	g13914.t5	exon	g13914.t5.exon4	33373986	33374238
chr_1	g13914	g13914.t5	cds	g13914.t5.CDS2	33373986	33374238
chr_1	g13914	g13914.t5	exon	g13914.t5.exon5	33374341	33375461
chr_1	g13914	g13914.t5	cds	g13914.t5.CDS3	33374341	33375461
chr_1	g13914	g13914.t5	TTS	g13914.t5	NA	NA

Sequences

>g13914.t5 Gene=g13914 Length=1975
CAGTTTGCGAGGCACTTTTGTGTGAGGCAGAAGTACATTTTTTTCTCTCTTTACAAAATC
ACAATCATGAACTTTTTGATTTGTAAAAAGTAAAAATATTGTGTCGTTCACAATCGTGTA
ATTTTTTTTCTCAATACATTAAAATCCAACAGGTATGGACATTTTGAGATATCATCGTGA
TTCTTGAGATAGTTCATTGTCAAAAGTTGTATACAGTGAGAGAGGAATAAAGAGAGTGAA
AGAGAAAAATCATCTGTTTCTGTGTACATTATTGAGACATTTATTTTTAAATGTGAAAAG
CATGTGTAAAATGACACGAGAATGTCTCTTTTGAACCGATTGTGCAAAATCATTTGCAAA
TTTGAGTGAAAAAGAGAGAGAGAGAGAGAAGGAAACTTTATGCAATAATAATATGGAGAA
TAATGAGCTGAAATCATCAGACTCTGCAGATGCAGAAGCAATAAATCGAGATCAAATACT
TGAAACACCATCGGAGCGTAGAATTCGCATCTTTTCTCTCAATATAATTTATTTTACAAT
GTTTTTAATGACACTTGGTTTTTCAATAATATTAACTGCCATTTGGCCATATATTGATAA
GTTAGATAGAGACGCTGGTAAAGTATTTATGGGTTGGATTGTTGCTGCTAATCCTCTAGG
ACAATTTATTTTCTCTCCCGTATTTGGATGGTGGGCAAATAAATCAAAATCGATAAGAAC
GCCATTTATCGTATCTATGATTATTTTTTGCGTCGCAAGTGCAGTCTATTCATGTCTGGA
CATTGTTGAAAATAATGTAAAATATTGGATGCTTGTTTGTAGATTTTTTGTTGGCGTCTC
ATCGGCAAACATTGTTATTTGTAGAAGCTATGTGTCAGCTGCTACTAAACTTAAGGAAAG
AACAAAGGCTGTGTCTTTATTAACACTTGCCCAAACACTTGGATTTATATTTGGTCCACT
TTTTCAAGCGGCATTTACTTCATTAGGAAGTGAAGGATATAAAATGTACTTTAATTTACC
ACTATCGATGTATACAGCACCAGGATGGCTCAATGTTTTCCTTGGCTTATTCAATTTGAT
ACTATTTCATCCTAAAATTTTCCAAGACAAAAGAGTCGCTGCACGTGAACAGATGTTAAT
TCAGGGAAAAGCTTCAGAAAAAGAAGCATGGAAAAATATTAAGCCTGATTACTTTGTTTC
ATGGGCACTCATATTTTCATTATTTGTATTTGTTTTTAATTTCGTATTGCTCGAAAGTAT
GGGAACAGCTTTAACAATGGAACAATTTGCATGGACAAAGAAACAAGCAATTGAATATAT
GGCGTATATTATGGGTATTGGCGGTTTGATAGCATGTGGTACATTTGTAGCAATATCACC
GCTCTGTAAGAAATTTAGAGAAAATGATGTGCTGATATATGGTGGCTTTTTTGTTATGAT
ATTATCGCGTATAACTCATATTCCCTACGGAAGTACAATACCAAAATTAGCTTTACCCAA
AGAATACACATTCGAAAATGGCACACATGTGATATTTGAAGAAGATGATCCTCACATTCT
CGGTTGTCCGGCATATGAGCAAAAGTGGTGTGAAACAACTCCAGCATTAGGATTCTATGA
ATTTATTTTAGGATACATTCTCACAAGTTTTGGCTATCCTATTGGCCTAACATTAATTCA
AACAATTTTCTCAAAAGTTCTTGGACCACGACCGCAAGGTGTTTGGATGAGTTTGATGAC
AAATGCCGGTTGTCTATCAAGAATTTTTGGACCAATTTGTGTATCAATGCTTTATGCTCG
CTTTGGCACAAGAATTACAATGTTATTTACTCTAATAATTATGATAATTCCAATGGTTTG
GCTTCTTTTCTTAAAAGACCGCATGCTGATTGAAGATTTTAAAGATAAAACAATTGAAAT
GGAAGAAAGAAATGGATTAAAACCAAATATACAAATTAATGATAACGATAGATGA

>g13914.t5 Gene=g13914 Length=520
MENNELKSSDSADAEAINRDQILETPSERRIRIFSLNIIYFTMFLMTLGFSIILTAIWPY
IDKLDRDAGKVFMGWIVAANPLGQFIFSPVFGWWANKSKSIRTPFIVSMIIFCVASAVYS
CLDIVENNVKYWMLVCRFFVGVSSANIVICRSYVSAATKLKERTKAVSLLTLAQTLGFIF
GPLFQAAFTSLGSEGYKMYFNLPLSMYTAPGWLNVFLGLFNLILFHPKIFQDKRVAAREQ
MLIQGKASEKEAWKNIKPDYFVSWALIFSLFVFVFNFVLLESMGTALTMEQFAWTKKQAI
EYMAYIMGIGGLIACGTFVAISPLCKKFRENDVLIYGGFFVMILSRITHIPYGSTIPKLA
LPKEYTFENGTHVIFEEDDPHILGCPAYEQKWCETTPALGFYEFILGYILTSFGYPIGLT
LIQTIFSKVLGPRPQGVWMSLMTNAGCLSRIFGPICVSMLYARFGTRITMLFTLIIMIIP
MVWLLFLKDRMLIEDFKDKTIEMEERNGLKPNIQINDNDR

Protein features from InterProScan

	Transcript	Database	ID	Name	Start	End	E.value
43	g13914.t5	CDD	cd17326	MFS_MFSD8	38	491	2.96508E-120
2	g13914.t5	PANTHER	PTHR23510:SF3	MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 8	25	491	5.1E-148
3	g13914.t5	PANTHER	PTHR23510	INNER MEMBRANE TRANSPORT PROTEIN YAJR	25	491	5.1E-148
1	g13914.t5	Pfam	PF07690	Major Facilitator Superfamily	39	345	4.9E-34
19	g13914.t5	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.	1	37	-
33	g13914.t5	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.	38	61	-
27	g13914.t5	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.	62	72	-
42	g13914.t5	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.	73	93	-
23	g13914.t5	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.	94	104	-
32	g13914.t5	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.	105	125	-
30	g13914.t5	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.	126	130	-
34	g13914.t5	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.	131	154	-
20	g13914.t5	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.	155	165	-
39	g13914.t5	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.	166	184	-
25	g13914.t5	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.	185	203	-
36	g13914.t5	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.	204	225	-
22	g13914.t5	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.	226	259	-
35	g13914.t5	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.	260	279	-
29	g13914.t5	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.	280	298	-
31	g13914.t5	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.	299	321	-
24	g13914.t5	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.	322	332	-
40	g13914.t5	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.	333	352	-
28	g13914.t5	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.	353	404	-
41	g13914.t5	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.	405	426	-
18	g13914.t5	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.	427	437	-
37	g13914.t5	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.	438	462	-
26	g13914.t5	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.	463	467	-
38	g13914.t5	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.	468	487	-
21	g13914.t5	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.	488	520	-
17	g13914.t5	ProSiteProfiles	PS50850	Major facilitator superfamily (MFS) profile.	35	492	11.973
16	g13914.t5	SUPERFAMILY	SSF103473	MFS general substrate transporter	35	491	5.89E-44
10	g13914.t5	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.	39	61	-
7	g13914.t5	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.	71	93	-
8	g13914.t5	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.	100	122	-
5	g13914.t5	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.	132	154	-
14	g13914.t5	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.	166	188	-
15	g13914.t5	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.	203	225	-
9	g13914.t5	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.	260	279	-
4	g13914.t5	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.	299	321	-
6	g13914.t5	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.	333	350	-
12	g13914.t5	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.	404	426	-
11	g13914.t5	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.	447	464	-
13	g13914.t5	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.	468	487	-

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID	TERM	ONTOLOGY
GO:0055085	transmembrane transport	BP
GO:0022857	transmembrane transporter activity	MF

KEGG

Orthology

This transcript has been annotated with K12307 (MFS transporter, ceroid-lipofuscinosis neuronal protein 7)

Pathway

This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.