General information for g14023.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Alcohol dehydrogenase class-3.
Parent gene
- The parent gene g14023 is located on chr_1: 34103849..34104511 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g14023 | g14023.t1 | TTS | g14023.t1 | 34102731 | 34102731 |
| chr_1 | g14023 | g14023.t1 | isoform | g14023.t1 | 34102827 | 34104511 |
| chr_1 | g14023 | g14023.t1 | exon | g14023.t1.exon1 | 34102827 | 34103108 |
| chr_1 | g14023 | g14023.t1 | cds | g14023.t1.CDS1 | 34102827 | 34103108 |
| chr_1 | g14023 | g14023.t1 | exon | g14023.t1.exon2 | 34103399 | 34103655 |
| chr_1 | g14023 | g14023.t1 | cds | g14023.t1.CDS2 | 34103399 | 34103655 |
| chr_1 | g14023 | g14023.t1 | exon | g14023.t1.exon3 | 34103722 | 34103781 |
| chr_1 | g14023 | g14023.t1 | cds | g14023.t1.CDS3 | 34103722 | 34103781 |
| chr_1 | g14023 | g14023.t1 | exon | g14023.t1.exon4 | 34103850 | 34104179 |
| chr_1 | g14023 | g14023.t1 | cds | g14023.t1.CDS4 | 34103850 | 34104179 |
| chr_1 | g14023 | g14023.t1 | exon | g14023.t1.exon5 | 34104242 | 34104314 |
| chr_1 | g14023 | g14023.t1 | cds | g14023.t1.CDS5 | 34104242 | 34104314 |
| chr_1 | g14023 | g14023.t1 | exon | g14023.t1.exon6 | 34104377 | 34104511 |
| chr_1 | g14023 | g14023.t1 | cds | g14023.t1.CDS6 | 34104377 | 34104511 |
| chr_1 | g14023 | g14023.t1 | TSS | g14023.t1 | 34104576 | 34104576 |
Sequences
>g14023.t1 Gene=g14023 Length=1137
ATGACTGAAGGGAAAGTAATTAGTTGTCGTGCAGCTGTTGCTTGGGAAGCTAACAAGCCA
TTGTCAATCGAAGAAATTCAAGTTCAACCACCAAAATCTGGTGAAGTTCGAGTAAAAATT
TTTGCAACCGGTGTTTGTCATACTGACGCATATACACTAAGTGGAATTGATCCAGAAGGA
CTTTTTCCGAGCATTCTTGGTCATGAAGGCGCTGGAATTGTGGAATCAGTTGGTGAAGGT
GTAACAAATTTTAAGGCAGGCGACCATGTTATTCCTCTTTATACACCACAATGCAAAGAG
TGTCAATATTGCAAAAGCAAAAAGACAAATTTGTGTCAAAAAATCCGCTTAACTCAAGGT
AAAGGTGTAATGCCAGATGGCACTTCACGTTTTACATGCAAAGACAAGCAATTATATCAT
TTTATGGGCTGCTCTACATTCTCTGAGTATACTGTCGTTAGTCAAATTTCTTTGTGCAAA
GTTGCCGAGTCTGCACCTCTTGAAAAAATATGCCTTCTCGGATGTGGCATACCAACCGGC
TATGGAGCTGCAGTTAATACAGCAGGTGTTGAAGAAGGAAGTGTTGTCGCCATTTGGGGT
CTTGGTGCAGTCGGTCTTGCTGTAATTGCAGGATGCAAAGATTTAAAAGCTAAGACAATC
ATTGGAATTGATATAAATGAATCTAAATTTGAAATAGCAAAATCCTTTGGTGTAACTGAC
TTCGTCAATCCAAAAAAATTGCCTGAAGGAAAAACAATTCAGGAATATCTTTTTGACAAT
TTTGATGGAGGTCCTGATTATACGTTTGAATGCATTGGAAATGTTCAAACAATGAGACAA
GCATTAGAATCAGCGCATAAAGGTTGGGGCGTCTCTGTAATTATAGGTGTTGCCGGTGCT
GGACAAGAAATTTCAACACGCCCGTTTCAACTTGTCACTGGTCGTGTATGGAAAGGAACA
GCCTTTGGAGGCTACAAAAGCGTAGAAAGTATACCAAAATTAGTTGATCGCTATCTCGCT
GGCAATTTTAAAATTGATGAATTCATCACTCATAATGTAAATCTTGATAAAATCAATGAA
GCTTTTGATTTAATGCATGAGGGAAAGAGCATTAGAACTATTGTTCATTTCAAATAG
>g14023.t1 Gene=g14023 Length=378
MTEGKVISCRAAVAWEANKPLSIEEIQVQPPKSGEVRVKIFATGVCHTDAYTLSGIDPEG
LFPSILGHEGAGIVESVGEGVTNFKAGDHVIPLYTPQCKECQYCKSKKTNLCQKIRLTQG
KGVMPDGTSRFTCKDKQLYHFMGCSTFSEYTVVSQISLCKVAESAPLEKICLLGCGIPTG
YGAAVNTAGVEEGSVVAIWGLGAVGLAVIAGCKDLKAKTIIGIDINESKFEIAKSFGVTD
FVNPKKLPEGKTIQEYLFDNFDGGPDYTFECIGNVQTMRQALESAHKGWGVSVIIGVAGA
GQEISTRPFQLVTGRVWKGTAFGGYKSVESIPKLVDRYLAGNFKIDEFITHNVNLDKINE
AFDLMHEGKSIRTIVHFK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g14023.t1 | CDD | cd08300 | alcohol_DH_class_III | 7 | 376 | 0.0 |
| 10 | g14023.t1 | Gene3D | G3DSA:3.90.180.10 | - | 12 | 372 | 8.4E-147 |
| 9 | g14023.t1 | Gene3D | G3DSA:3.40.50.720 | - | 180 | 320 | 8.4E-147 |
| 3 | g14023.t1 | PANTHER | PTHR43880:SF21 | S-(HYDROXYMETHYL)GLUTATHIONE DEHYDROGENASE | 3 | 376 | 1.9E-196 |
| 4 | g14023.t1 | PANTHER | PTHR43880 | ALCOHOL DEHYDROGENASE | 3 | 376 | 1.9E-196 |
| 2 | g14023.t1 | Pfam | PF08240 | Alcohol dehydrogenase GroES-like domain | 34 | 160 | 2.9E-24 |
| 1 | g14023.t1 | Pfam | PF00107 | Zinc-binding dehydrogenase | 203 | 327 | 7.2E-21 |
| 8 | g14023.t1 | ProSitePatterns | PS00059 | Zinc-containing alcohol dehydrogenases signature. | 67 | 81 | - |
| 7 | g14023.t1 | SUPERFAMILY | SSF50129 | GroES-like | 2 | 183 | 1.29E-76 |
| 5 | g14023.t1 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 166 | 341 | 1.31E-41 |
| 6 | g14023.t1 | SUPERFAMILY | SSF50129 | GroES-like | 327 | 377 | 1.77E-12 |
| 11 | g14023.t1 | TIGRFAM | TIGR02818 | adh_III_F_hyde: S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase | 9 | 377 | 5.4E-186 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006069 | ethanol oxidation | BP |
| GO:0008270 | zinc ion binding | MF |
| GO:0055114 | NA | NA |
| GO:0051903 | S-(hydroxymethyl)glutathione dehydrogenase activity | MF |
| GO:0016491 | oxidoreductase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K00121 (S-(hydroxymethyl)glutathione dehydrogenase / alcohol dehydrogenase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
