General information for g14108.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Prolyl 4-hydroxylase subunit alpha-2.
Parent gene
- The parent gene g14108 is located on chr_1: 34731941..34733598 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g14108 | g14108.t2 | TSS | g14108.t2 | 34731437 | 34731437 |
| chr_1 | g14108 | g14108.t2 | isoform | g14108.t2 | 34731458 | 34732818 |
| chr_1 | g14108 | g14108.t2 | exon | g14108.t2.exon1 | 34731458 | 34731627 |
| chr_1 | g14108 | g14108.t2 | cds | g14108.t2.CDS1 | 34731458 | 34731627 |
| chr_1 | g14108 | g14108.t2 | exon | g14108.t2.exon2 | 34731936 | 34732818 |
| chr_1 | g14108 | g14108.t2 | cds | g14108.t2.CDS2 | 34731936 | 34732818 |
| chr_1 | g14108 | g14108.t2 | TTS | g14108.t2 | 34733629 | 34733629 |
Sequences
>g14108.t2 Gene=g14108 Length=1053
ATGAAACGTTTTATGATTTTTGTGATAGTAATTAATTTAGTTTTTACCAATTGTGAGATA
TTCTCAGGCAGTAATGCTCTGGAAAAATTTGTTGAAAATGAAGAAAAGATATTGAGTGAA
TTTGAAGTTTTTATTAAAAAATTGCAAGATGAAAATGATTATTTAATGAGTAAACTCCAA
AGTTGGAAAGATGAATATTCGCAAGTTAAGAAAGATCCCATAAAATACATAAATAACCCA
ATTAACGCATTTCATGTAATTAAGCGTACAACTGAAGACATTGACTTATTTGAGTTACGA
ATTAAGAAATTAATTGATCAATTCACCAGCAGCATTTCAAGCATAAGAGCAACAAATAAT
GATCTCACTGGTACCGTTGAAGGAATAATGCGATTGCAGACAATTTATCATTTAAAAACT
GCTGATATAATAAATGGAATAATCGATGGAGATAAAACAAATGAGAAACTTAAACCTCAT
GATCTTTTCACAATAGCACGTGAAGGATTAAAAATTCAAGATAAATCTTATTTTGTGCAT
GAATATTTATACCATTTACAAAAGTCATTAGAAAATAATGAAGATGAATTTGATGAAGTT
GATTTAGATGAATTTTCTGAAATTGTCGAAATTACTTTAAATTCTACTATGATTGATCCA
TTTGATGAATCTTTTGAAAAGAATGGAGCTTATACTGTTCATAAAGAAAATGTCATTTAT
AGTCAAGGATGTCGTCAGAAAATTCAAAAAACTCCAAAAGAAGCAAAAGATCTCAAATGT
CGTTATGTGTCGAATTCTGCATTTTCAAAAATTGCTCCATTTAAAGTCATCGAAGCTGAC
CATCATTCAAATTTGGTTGTTTTTCTTGATGTCATTTCTGATAATGAAATTGAAATGATT
AAAAATATTTCAAGTTCTAAATTAGGTAGAGGTGGAGTTTTTGTTGAAGAAAAAAGTACA
AAAACAGGATTTTCTAACACTCGAGTTTCACAAATTAGTTGGCATTATGAACATGAACAT
GAAATTGTTGCCAGAATTTCTAGACGAGTTGAA
>g14108.t2 Gene=g14108 Length=351
MKRFMIFVIVINLVFTNCEIFSGSNALEKFVENEEKILSEFEVFIKKLQDENDYLMSKLQ
SWKDEYSQVKKDPIKYINNPINAFHVIKRTTEDIDLFELRIKKLIDQFTSSISSIRATNN
DLTGTVEGIMRLQTIYHLKTADIINGIIDGDKTNEKLKPHDLFTIAREGLKIQDKSYFVH
EYLYHLQKSLENNEDEFDEVDLDEFSEIVEITLNSTMIDPFDESFEKNGAYTVHKENVIY
SQGCRQKIQKTPKEAKDLKCRYVSNSAFSKIAPFKVIEADHHSNLVVFLDVISDNEIEMI
KNISSSKLGRGGVFVEEKSTKTGFSNTRVSQISWHYEHEHEIVARISRRVE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g14108.t2 | Coils | Coil | Coil | 31 | 65 | - |
| 8 | g14108.t2 | Coils | Coil | Coil | 87 | 107 | - |
| 5 | g14108.t2 | Gene3D | G3DSA:1.25.40.10 | - | 119 | 235 | 5.1E-12 |
| 6 | g14108.t2 | Gene3D | G3DSA:2.60.120.620 | q2cbj1_9rhob like domain | 276 | 351 | 1.3E-5 |
| 2 | g14108.t2 | PANTHER | PTHR10869:SF151 | FI16820P1-RELATED | 39 | 351 | 2.5E-33 |
| 3 | g14108.t2 | PANTHER | PTHR10869 | PROLYL 4-HYDROXYLASE ALPHA SUBUNIT | 39 | 351 | 2.5E-33 |
| 1 | g14108.t2 | Pfam | PF08336 | Prolyl 4-Hydroxylase alpha-subunit, N-terminal region | 26 | 150 | 5.8E-24 |
| 10 | g14108.t2 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 11 | g14108.t2 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 12 | g14108.t2 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 11 | - |
| 13 | g14108.t2 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 12 | 16 | - |
| 9 | g14108.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 351 | - |
| 4 | g14108.t2 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 20 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016702 | oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen | MF |
| GO:0005783 | endoplasmic reticulum | CC |
| GO:0005515 | protein binding | MF |
| GO:0004656 | procollagen-proline 4-dioxygenase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed