General information for g14112.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Matrix metalloproteinase-16.
Parent gene
- The parent gene g14112 is located on chr_1: 34749015..34764841 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g14112 | g14112.t1 | TTS | g14112.t1 | 34747221 | 34747221 |
| chr_1 | g14112 | g14112.t1 | isoform | g14112.t1 | 34747432 | 34764841 |
| chr_1 | g14112 | g14112.t1 | exon | g14112.t1.exon1 | 34747432 | 34747781 |
| chr_1 | g14112 | g14112.t1 | cds | g14112.t1.CDS1 | 34747432 | 34747781 |
| chr_1 | g14112 | g14112.t1 | exon | g14112.t1.exon2 | 34748299 | 34748418 |
| chr_1 | g14112 | g14112.t1 | cds | g14112.t1.CDS2 | 34748299 | 34748418 |
| chr_1 | g14112 | g14112.t1 | exon | g14112.t1.exon3 | 34748627 | 34748764 |
| chr_1 | g14112 | g14112.t1 | cds | g14112.t1.CDS3 | 34748627 | 34748764 |
| chr_1 | g14112 | g14112.t1 | exon | g14112.t1.exon4 | 34748845 | 34749450 |
| chr_1 | g14112 | g14112.t1 | cds | g14112.t1.CDS4 | 34748845 | 34749450 |
| chr_1 | g14112 | g14112.t1 | exon | g14112.t1.exon5 | 34749510 | 34749660 |
| chr_1 | g14112 | g14112.t1 | cds | g14112.t1.CDS5 | 34749510 | 34749660 |
| chr_1 | g14112 | g14112.t1 | exon | g14112.t1.exon6 | 34749717 | 34749783 |
| chr_1 | g14112 | g14112.t1 | cds | g14112.t1.CDS6 | 34749717 | 34749783 |
| chr_1 | g14112 | g14112.t1 | exon | g14112.t1.exon7 | 34751167 | 34751313 |
| chr_1 | g14112 | g14112.t1 | cds | g14112.t1.CDS7 | 34751167 | 34751313 |
| chr_1 | g14112 | g14112.t1 | exon | g14112.t1.exon8 | 34755510 | 34755796 |
| chr_1 | g14112 | g14112.t1 | cds | g14112.t1.CDS8 | 34755510 | 34755796 |
| chr_1 | g14112 | g14112.t1 | exon | g14112.t1.exon9 | 34764713 | 34764841 |
| chr_1 | g14112 | g14112.t1 | cds | g14112.t1.CDS9 | 34764713 | 34764841 |
| chr_1 | g14112 | g14112.t1 | TSS | g14112.t1 | NA | NA |
Sequences
>g14112.t1 Gene=g14112 Length=1995
ATGAAGCATATCCTGGGCTCCATCGCATTCTTCATCTGTGTCATAAATATTGAATCTCAT
CCGAGACATGAAAGAAGCGACAAGCAGAGCAATAACAATAAATTACTGACTGACTATAGT
GATGATGTGCAAAACTATTTAATGGAATTCGGTTATTTGCCGAAATCAAACAGTGAGACT
GGAAATTTGCGCATTGAAGAGCAACTTAGAAATGCAATTAGAAGACTTCAAGCATTTGCT
GGAATTCCACAGACAGGTAAAATGGATGCAAAAACAAACCTGTTGATGAAATCGCCACGT
TGCGGTGTGCCTGATGATTTTGATTCAAGTGATTTCAAACAACGACAGCGAACGCGATTC
AAACGTTTTGTTATCAACAATGGACAGAAATGGGAAAACACAAATCTCACGTGGAGCTTT
GTGAATCAAACTATGACAAATATTGACGCTGGAATGGCGCGAAGAATATTTCATGAAGCA
CTAGCAATATGGTCAAAAGATTCAAAATTAAATTTCCGTGAGGTCTATGATCCAAATGCT
GACATACAGATAATGTTCGCACAAGAAGATCATGGAGATGGTTTTAGATTTGATGGTCCA
GGCAATGTTTTAGCTCATGCCTTTTATCCAGGAAGTGGTAGAGGTGGTGATGCACATTTT
GATCAAGATGAAGCTTGGCATTTAGATGATAGAACAATAGAACATAGTGGAACAAGTTTG
AAAAATGTTGCAATTCATGAATTTGGTCACAGTTTGGGTCTTGGACATTCAAGTGTAAAA
GGAGCTGTGATGTTTCCATGGTATCATGGTTATAGAGGTGATGGTGATTTACCAGAAGAT
GACAGACTTGCAATTCAAACACTTTATGGTGTACGTGATGGAGCAAGGCAATGGGGACCA
AATCCAAGGCGGCATCATTACACTTCATCAACGCCTAGAACGACAACAACAAGAACAACA
ACAACTTATAGACCTCGTCATTATACACCAGATCAACGACATTATCCAGATCAGCCTAGA
AGAAATCCAAATGAATATCCATCACGTTATCCAAGTTATTCAACTACAACTACAACAACA
ACAAGAAGACCAACATATCATCACAATAATAGAAATAATCATCATCATCAGCATCATAAT
AAACAACATACAAATCATGGACATGACAAAAAGCCAGACACATGCAGCACATCTTATGAC
GCAATTACAATGATTCGTGGTGAAATATTCATCTTCAAAGGACGATATTTTTGGCGAATT
GGTCAAGATGGCTTACAGCCTGGTTATCCACATGAAATTAGTAAAATGTGGAGAGATTTA
CCGCACAACATGACACATATTGATGCAGTATATGAAAATAAAAAACATCAAATTGTCTTT
TTCATTGGAAATCAATATTATATTTTCAACACACAACATTTACTTGAAACACAACCACTG
AGCACACTCGGACTGCCAGAGAATTTGAAGAAAATTGATGCGGCTCTTGTTTGGGGTCAT
AATAATCGCACGTACTTCTATAGCGGAACAAAGTATTGGCGATTTGATGAAGATGAAATG
CATGTCGAGCTTGATTATCCACGTGATATGTCAATGTGGCGTGGAATTGGATACGATATT
GATTCAGCTTTTCAATATAAAGATGGAAAAACATATTTCTTCAAAGGAAGCGGCTATTGG
CAATTTGATGATATGAAAATGAGAGTCGTACATGATAGACAGAAAAAGTCTGCTTATAGA
TGGATGGGCTGCAAACAGAAACATGAAAGATTGGATGATGATTCGAATGAGAGACGACAA
TATTGGAGAAATCCAAGGAGAGATGAAGAAACGTCGGATGTTGACGACGATGACTTGTAT
AGCAATGAAGATATAGATATTGTAACTTCCACAGCATCAGCTTCCATTTTATTAGAATTA
ATTAGTTTTAAGATTCTTTTATCAATTAGTTTAGGAGCATTTTTAAATTCAATAATCACA
AGGAGAATGACTTGA
>g14112.t1 Gene=g14112 Length=664
MKHILGSIAFFICVINIESHPRHERSDKQSNNNKLLTDYSDDVQNYLMEFGYLPKSNSET
GNLRIEEQLRNAIRRLQAFAGIPQTGKMDAKTNLLMKSPRCGVPDDFDSSDFKQRQRTRF
KRFVINNGQKWENTNLTWSFVNQTMTNIDAGMARRIFHEALAIWSKDSKLNFREVYDPNA
DIQIMFAQEDHGDGFRFDGPGNVLAHAFYPGSGRGGDAHFDQDEAWHLDDRTIEHSGTSL
KNVAIHEFGHSLGLGHSSVKGAVMFPWYHGYRGDGDLPEDDRLAIQTLYGVRDGARQWGP
NPRRHHYTSSTPRTTTTRTTTTYRPRHYTPDQRHYPDQPRRNPNEYPSRYPSYSTTTTTT
TRRPTYHHNNRNNHHHQHHNKQHTNHGHDKKPDTCSTSYDAITMIRGEIFIFKGRYFWRI
GQDGLQPGYPHEISKMWRDLPHNMTHIDAVYENKKHQIVFFIGNQYYIFNTQHLLETQPL
STLGLPENLKKIDAALVWGHNNRTYFYSGTKYWRFDEDEMHVELDYPRDMSMWRGIGYDI
DSAFQYKDGKTYFFKGSGYWQFDDMKMRVVHDRQKKSAYRWMGCKQKHERLDDDSNERRQ
YWRNPRRDEETSDVDDDDLYSNEDIDIVTSTASASILLELISFKILLSISLGAFLNSIIT
RRMT
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 29 | g14112.t1 | CDD | cd04278 | ZnMc_MMP | 130 | 290 | 4.51357E-67 |
| 28 | g14112.t1 | CDD | cd00094 | HX | 392 | 584 | 4.75028E-57 |
| 18 | g14112.t1 | Gene3D | G3DSA:3.40.390.10 | Collagenase (Catalytic Domain) | 28 | 295 | 1.3E-85 |
| 19 | g14112.t1 | Gene3D | G3DSA:2.110.10.10 | Hemopexin | 392 | 587 | 5.2E-69 |
| 38 | g14112.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 294 | 393 | - |
| 41 | g14112.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 303 | 326 | - |
| 37 | g14112.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 345 | 366 | - |
| 39 | g14112.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 367 | 385 | - |
| 36 | g14112.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 595 | 610 | - |
| 40 | g14112.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 595 | 615 | - |
| 7 | g14112.t1 | PANTHER | PTHR10201 | MATRIX METALLOPROTEINASE | 40 | 550 | 1.5E-128 |
| 8 | g14112.t1 | PANTHER | PTHR10201:SF169 | MATRIX METALLOPROTEINASE-16-LIKE PROTEIN | 40 | 550 | 1.5E-128 |
| 27 | g14112.t1 | PIRSF | PIRSF001191 | MMP_stromelysin | 2 | 610 | 1.2E-147 |
| 9 | g14112.t1 | PRINTS | PR00138 | Matrixin signature | 96 | 109 | 2.2E-37 |
| 13 | g14112.t1 | PRINTS | PR00138 | Matrixin signature | 157 | 172 | 2.2E-37 |
| 12 | g14112.t1 | PRINTS | PR00138 | Matrixin signature | 180 | 208 | 2.2E-37 |
| 10 | g14112.t1 | PRINTS | PR00138 | Matrixin signature | 243 | 268 | 2.2E-37 |
| 11 | g14112.t1 | PRINTS | PR00138 | Matrixin signature | 277 | 290 | 2.2E-37 |
| 1 | g14112.t1 | Pfam | PF01471 | Putative peptidoglycan binding domain | 42 | 92 | 1.2E-8 |
| 2 | g14112.t1 | Pfam | PF00413 | Matrixin | 130 | 290 | 8.1E-52 |
| 6 | g14112.t1 | Pfam | PF00045 | Hemopexin | 400 | 442 | 9.0E-10 |
| 5 | g14112.t1 | Pfam | PF00045 | Hemopexin | 447 | 472 | 1.5E-4 |
| 3 | g14112.t1 | Pfam | PF00045 | Hemopexin | 492 | 537 | 1.8E-10 |
| 4 | g14112.t1 | Pfam | PF00045 | Hemopexin | 540 | 584 | 4.5E-8 |
| 22 | g14112.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 19 | - |
| 23 | g14112.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 24 | g14112.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 15 | - |
| 26 | g14112.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 19 | - |
| 21 | g14112.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 20 | 635 | - |
| 25 | g14112.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 636 | 659 | - |
| 20 | g14112.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 660 | 664 | - |
| 44 | g14112.t1 | ProSiteProfiles | PS51642 | Hemopexin repeat profile. | 392 | 440 | 13.263 |
| 43 | g14112.t1 | ProSiteProfiles | PS51642 | Hemopexin repeat profile. | 444 | 487 | 7.664 |
| 45 | g14112.t1 | ProSiteProfiles | PS51642 | Hemopexin repeat profile. | 489 | 536 | 13.022 |
| 42 | g14112.t1 | ProSiteProfiles | PS51642 | Hemopexin repeat profile. | 537 | 584 | 12.836 |
| 35 | g14112.t1 | SMART | SM00235 | col_5 | 127 | 291 | 6.2E-36 |
| 33 | g14112.t1 | SMART | SM00120 | HX_rpt_3 | 399 | 442 | 9.0E-8 |
| 31 | g14112.t1 | SMART | SM00120 | HX_rpt_3 | 447 | 487 | 2.0E-4 |
| 32 | g14112.t1 | SMART | SM00120 | HX_rpt_3 | 492 | 538 | 3.7E-9 |
| 34 | g14112.t1 | SMART | SM00120 | HX_rpt_3 | 540 | 584 | 7.1E-7 |
| 14 | g14112.t1 | SUPERFAMILY | SSF47090 | PGBD-like | 43 | 105 | 1.73E-17 |
| 16 | g14112.t1 | SUPERFAMILY | SSF55486 | Metalloproteases (zincins), catalytic domain | 123 | 292 | 3.31E-46 |
| 15 | g14112.t1 | SUPERFAMILY | SSF50923 | Hemopexin-like domain | 389 | 584 | 8.11E-62 |
| 17 | g14112.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 19 | - |
| 30 | g14112.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 637 | 659 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0031012 | extracellular matrix | CC |
| GO:0008237 | metallopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0004222 | metalloendopeptidase activity | MF |
| GO:0008270 | zinc ion binding | MF |
KEGG
Orthology
- This transcript has been annotated with K07995 (matrix metalloproteinase-15 (membrane-inserted) [EC:3.4.24.-])
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
