General information for g14117.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Probable ADP-dependent glucokinase.
Parent gene
- The parent gene g14117 is located on chr_1: 34793527..34795090 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g14117 | g14117.t2 | isoform | g14117.t2 | 34793527 | 34795090 |
| chr_1 | g14117 | g14117.t2 | exon | g14117.t2.exon1 | 34793527 | 34794599 |
| chr_1 | g14117 | g14117.t2 | cds | g14117.t2.CDS1 | 34793527 | 34794599 |
| chr_1 | g14117 | g14117.t2 | exon | g14117.t2.exon2 | 34794673 | 34795090 |
| chr_1 | g14117 | g14117.t2 | cds | g14117.t2.CDS2 | 34794673 | 34795090 |
| chr_1 | g14117 | g14117.t2 | TSS | g14117.t2 | NA | NA |
| chr_1 | g14117 | g14117.t2 | TTS | g14117.t2 | NA | NA |
Sequences
>g14117.t2 Gene=g14117 Length=1491
ATGATTCCCTTCTTCAAATATTTCACTATATCGTCCTTCATTGCTCTATTTGCAATAATT
TTTCAAGCCTATTTTGAGAGCGGTCAATTGCAAGAAGTGACTGAAGTTCTATCAAGTTTA
ATGAAATTAGAAAACGTTAATCGTAAATCGATACCAAAAGTTATAATCGGTTATGGAAGT
TGCAGTGACTTGACTGTTCGTGCTGTTGACTTTTTAAATTATTCTGAAAATTTTAAAGAA
AGTTTTTCACAACGAGATCAGAATGACGACGAAATCAATACATTTGATGATTTTATGAAA
AGTTTCCTTTACTATTTCTCAAAAGGAGCAGCAGCTGAAAGATATACACCAAATAAAGAA
ATGTTTTTGAATTTGGTGAACATTGCTAAAAAACATGAATCGCATCGATGGGAATTAGCA
GTAATGGGAACTAGATTTTTAATTGAAAAGACTGAAGTTTTGCTTGCAGCTACAATGTCT
GATAAACAAAAGACTCATTTGATTAATGGAATAGATTTAGTAGATTTTAAACCAACAAAA
GATTTTGTGGATGACATTCATTTAATTTTAGAATATAAAACTGGAGAGCGATTTGGAGAA
CATGTAGCACCTCGAGCTAATCGCTATATCATACATTCAGATGCAAATAATCCTTTGATA
ATATCATTAGAATTACTCAAACCTAATGCTCTAAAAGGAGATCTTTTTGTTGTTTCAGGA
CTGCAAATGATGGATAATTATCCTTTTTCTTCACCAACTCTTAGAAGCGAAAGACTTGAA
GCTGTTAAAAATCAAATAATCAGTCTTGATAAGAAGACACTGACTCATTTTGAAATGGCA
TCTTTTGTTGAAATTGAGCTATTGAATGATTTGACTAAATATATTTTACCATATTCAGAC
TCAATTGGATTGAATGAACAAGAAATTGACAATTTATTGCATGTTCTAGAATCTGGAAAA
ATTAGTTTAGTTGCAAATTCTAATCCGAGAGTTGCAACAACATTAGATCAAATGCGTAAA
GTTTTTAAAATTCTTAATCGAAATTATTTTGCTAATAGAGACACTGATAAAAATGTTCGC
ATGTTGAGTAGAATTCACATTCATACATTAGCATTCCAATTAATTTTAAATGTCAAAGAT
AGTAAATGGAAAAATATCAAAACTGCAGCAGCAAAATCTTCTCTTATAGCACACCGTCAT
GTTTGTCAAACAAGTTATGTTAATCCAGATAATGCAATTTTAGTATTAGACGATAGTTTT
TCAACTTCAATTGAGGAAAATGCCAAAACTGACGATGTGAGACCAAAACGTATCGATGTT
AAAAAATCTGGCTCTGCAATTTCATGTTGGATTGAAAAATTAAAAGTTGATGAAAAGAAT
TTGGTTGAAGTGAAAATTTGCGTTGCACCAGTTTTAGTCTGTCGTGAGGCAAAAAAGACA
GTTGGTGCTGGCGATAATATTTCAGCTTCAGGTCTAGCAATGCAAATCTAA
>g14117.t2 Gene=g14117 Length=496
MIPFFKYFTISSFIALFAIIFQAYFESGQLQEVTEVLSSLMKLENVNRKSIPKVIIGYGS
CSDLTVRAVDFLNYSENFKESFSQRDQNDDEINTFDDFMKSFLYYFSKGAAAERYTPNKE
MFLNLVNIAKKHESHRWELAVMGTRFLIEKTEVLLAATMSDKQKTHLINGIDLVDFKPTK
DFVDDIHLILEYKTGERFGEHVAPRANRYIIHSDANNPLIISLELLKPNALKGDLFVVSG
LQMMDNYPFSSPTLRSERLEAVKNQIISLDKKTLTHFEMASFVEIELLNDLTKYILPYSD
SIGLNEQEIDNLLHVLESGKISLVANSNPRVATTLDQMRKVFKILNRNYFANRDTDKNVR
MLSRIHIHTLAFQLILNVKDSKWKNIKTAAAKSSLIAHRHVCQTSYVNPDNAILVLDDSF
STSIEENAKTDDVRPKRIDVKKSGSAISCWIEKLKVDEKNLVEVKICVAPVLVCREAKKT
VGAGDNISASGLAMQI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g14117.t2 | Gene3D | G3DSA:3.40.1190.20 | - | 194 | 496 | 1.9E-41 |
| 2 | g14117.t2 | PANTHER | PTHR21208:SF1 | ADP-DEPENDENT GLUCOKINASE 2 | 11 | 496 | 2.1E-141 |
| 3 | g14117.t2 | PANTHER | PTHR21208 | ADP-DEPENDENT GLUCOKINASE | 11 | 496 | 2.1E-141 |
| 1 | g14117.t2 | Pfam | PF04587 | ADP-specific Phosphofructokinase/Glucokinase conserved region | 53 | 493 | 2.6E-121 |
| 8 | g14117.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 6 | - |
| 10 | g14117.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 25 | - |
| 9 | g14117.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 26 | 496 | - |
| 7 | g14117.t2 | ProSiteProfiles | PS51255 | ADP-dependent kinase (ADPK) domain profile. | 31 | 496 | 57.576 |
| 5 | g14117.t2 | SUPERFAMILY | SSF53613 | Ribokinase-like | 48 | 495 | 1.23E-95 |
| 4 | g14117.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 25 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016773 | phosphotransferase activity, alcohol group as acceptor | MF |
| GO:0005975 | carbohydrate metabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K08074 (ADP-dependent glucokinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed