General information for g14135.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Crossover junction endonuclease MUS81.
Parent gene
- The parent gene g14135 is located on chr_1: 34851269..34851991 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g14135 | g14135.t1 | isoform | g14135.t1 | 34850063 | 34851991 |
| chr_1 | g14135 | g14135.t1 | exon | g14135.t1.exon1 | 34850063 | 34850371 |
| chr_1 | g14135 | g14135.t1 | cds | g14135.t1.CDS1 | 34850063 | 34850371 |
| chr_1 | g14135 | g14135.t1 | exon | g14135.t1.exon2 | 34850433 | 34851783 |
| chr_1 | g14135 | g14135.t1 | cds | g14135.t1.CDS2 | 34850433 | 34851783 |
| chr_1 | g14135 | g14135.t1 | exon | g14135.t1.exon3 | 34851849 | 34851991 |
| chr_1 | g14135 | g14135.t1 | cds | g14135.t1.CDS3 | 34851849 | 34851991 |
| chr_1 | g14135 | g14135.t1 | TSS | g14135.t1 | NA | NA |
| chr_1 | g14135 | g14135.t1 | TTS | g14135.t1 | NA | NA |
Sequences
>g14135.t1 Gene=g14135 Length=1803
ATGGATTCTAAAAGAATTAAAATTTTACAAAAGAATCCGAATGCTCTTTTTAAACAATGG
CTACGAGAATTTATTGAAGATGCTGTCAAGAAAAAGAAAAAAATCAGTAAAACTTATCAA
AAAGCTTTGGATTCTTTAGAAAGATATCCTTTGACATTATATAGTGGACATGATTGTGCT
ATTTTAGAGGGTTTTGGTCCAAAAATATGTGAATTGCTCGAGGAAAAATTGCAAAAGTAT
TTCGCAAATAATTTTGACGATCATGAATTGAGAAATCTTTGTTATAAAGACAAAATTGCA
TGCATTCGTAAGAGAGAACGTGAAGAACTTCAAGAATTAATTAAAGATATTGAAGCCGCA
AATTTAATAGATGAATCGACAATGATTGCTAATTTAAGGAGAACTTTAAGCACAATTGAA
GAGCATAATGACAAAAATGATGGTGAAGTTGAAGAAATAACAATGAGTGAGATAGTACCT
GAAAACGATAAGAAATCAGAAAAGGAAAAAATCAATTTTCAAATCATAGATGAAGAAAAT
TCTAATAGTTCTGATGATTCATTAGATAGACTTCTTAAAAAATATGATCCAAAAGCGGGT
GAAGAAAGGAAGAAAAAAAAGAAACTCGAAAAGAGAAAACCATTAACGACAGTTGATTCA
CCAGTATCAACACCTAAATTTATGAAATTTAAATCTCTTAATTCGATTCAGCATTTGGCA
GGACCATCATATGCAAGTTCACCAATTTCAAAATTTTTAGATGTCGAAACAAGTGGTTCT
CGTAAACAATTAAATTTGGTAGAAGAAGAAGATGAGTTTGATCGACTTGTAACAAAATAT
GATGATAACAAAATGAATGATGTTCTACCTTCTCCTATTGTGCCTGTCAAGAGAAAAATT
ACAAAAGAGAAAGCACTTTTTATTCAACAGAAAATTACTCAGCCATTGCCTGTGGTTGAA
ACACAACATGAAGAAAAAGAGGAAGAAGAAAAGTTTGATTACATTTCTATAGACGATATT
GAACCATCTGAATTTGAAATCATTTTACTAATTGATGTTGGTGAATCAAAAATTTCATCA
AAAGACATTCAAGCCAAACTTCGAGAATTTAATGTTAAAAGTGATGTAAGAAAATTGCAT
GTGGGAGATTTTGTATGGATCGCAAAGAATAAAATGAACAAGTCAAAAGAGCTTGTTCTT
CCTTATATTGTTGAAAGGAAAAGAATTGATGATCTTTCGAGCAGTATAAAAGATGGGAGA
TTTCATGAACAAAAATTTCGTCTCAGACATTGTGGTATTGATAATGTAATATATCTGATA
GAAAATTATATGAAAGGAGGTAAAGTTCAGTGTGGTCTTCCATTCACTACACTCTTGCAA
GCAGCATCAAACACTCAAATTCAAAATAAATTCACTGTGAAATTTACGGAGAGTAGTGAT
CATTCTGGAATGTATTTAGCTATTATGACTTCATTTATTGAAAATATTTTTAGGAATAAG
AAACCAATGAAATATAATCTATTGGAATTTACAACATTCAATCAAGCATCAGTGAAACAA
AGGAGACTGACTGTTCGTGAAACATTTATAAAACAATTACTTGCATTAAAAGGTCTTTCC
GTTGACATTGCACTTGAAATTACCAAATTTTATCCTACACCCTCACATCTGTATGAAAAA
TATTTGACTTTAGATAAAAGTGAGGGCGAAGCACTTTTATCAAAATTAACGATAGGCGAT
TTAAAACGTAAAATTCCATCGACCATCAGTAAAATCATTTATCATTTTTATATGCACAAA
TAA
>g14135.t1 Gene=g14135 Length=600
MDSKRIKILQKNPNALFKQWLREFIEDAVKKKKKISKTYQKALDSLERYPLTLYSGHDCA
ILEGFGPKICELLEEKLQKYFANNFDDHELRNLCYKDKIACIRKREREELQELIKDIEAA
NLIDESTMIANLRRTLSTIEEHNDKNDGEVEEITMSEIVPENDKKSEKEKINFQIIDEEN
SNSSDDSLDRLLKKYDPKAGEERKKKKKLEKRKPLTTVDSPVSTPKFMKFKSLNSIQHLA
GPSYASSPISKFLDVETSGSRKQLNLVEEEDEFDRLVTKYDDNKMNDVLPSPIVPVKRKI
TKEKALFIQQKITQPLPVVETQHEEKEEEEKFDYISIDDIEPSEFEIILLIDVGESKISS
KDIQAKLREFNVKSDVRKLHVGDFVWIAKNKMNKSKELVLPYIVERKRIDDLSSSIKDGR
FHEQKFRLRHCGIDNVIYLIENYMKGGKVQCGLPFTTLLQAASNTQIQNKFTVKFTESSD
HSGMYLAIMTSFIENIFRNKKPMKYNLLEFTTFNQASVKQRRLTVRETFIKQLLALKGLS
VDIALEITKFYPTPSHLYEKYLTLDKSEGEALLSKLTIGDLKRKIPSTISKIIYHFYMHK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g14135.t1 | Coils | Coil | Coil | 103 | 123 | - |
| 12 | g14135.t1 | Gene3D | G3DSA:1.10.150.110 | DNA polymerase beta | 13 | 84 | 9.6E-25 |
| 11 | g14135.t1 | Gene3D | G3DSA:3.40.50.10130 | - | 376 | 538 | 1.2E-53 |
| 10 | g14135.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 143 | 166 | - |
| 9 | g14135.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 194 | 217 | - |
| 2 | g14135.t1 | PANTHER | PTHR13451 | CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81 | 11 | 243 | 3.1E-81 |
| 4 | g14135.t1 | PANTHER | PTHR13451:SF0 | CROSSOVER JUNCTION ENDONUCLEASE MUS81 | 11 | 243 | 3.1E-81 |
| 3 | g14135.t1 | PANTHER | PTHR13451 | CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81 | 180 | 597 | 3.1E-81 |
| 5 | g14135.t1 | PANTHER | PTHR13451:SF0 | CROSSOVER JUNCTION ENDONUCLEASE MUS81 | 180 | 597 | 3.1E-81 |
| 1 | g14135.t1 | Pfam | PF02732 | ERCC4 domain | 351 | 487 | 1.6E-21 |
| 8 | g14135.t1 | SMART | SM00891 | ERCC4_2 | 348 | 444 | 9.3E-25 |
| 7 | g14135.t1 | SUPERFAMILY | SSF47802 | DNA polymerase beta, N-terminal domain-like | 9 | 79 | 5.62E-12 |
| 6 | g14135.t1 | SUPERFAMILY | SSF52980 | Restriction endonuclease-like | 345 | 492 | 3.22E-27 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003677 | DNA binding | MF |
| GO:0004518 | nuclease activity | MF |
KEGG
Orthology
- This transcript has been annotated with K08991 (crossover junction endonuclease MUS81 [EC:3.1.22.-])
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
