General information for g1425.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Neutral alpha-glucosidase AB.
Parent gene
- The parent gene g1425 is located on chr_3: 10894079..10896799 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g1425 | g1425.t1 | TSS | g1425.t1 | 10894055 | 10894055 |
| chr_3 | g1425 | g1425.t1 | isoform | g1425.t1 | 10894079 | 10896799 |
| chr_3 | g1425 | g1425.t1 | exon | g1425.t1.exon1 | 10894079 | 10896799 |
| chr_3 | g1425 | g1425.t1 | cds | g1425.t1.CDS1 | 10894079 | 10896799 |
| chr_3 | g1425 | g1425.t1 | TTS | g1425.t1 | 10896881 | 10896881 |
Sequences
>g1425.t1 Gene=g1425 Length=2721
ATGAAGTTGAAACTTTTGTTATTTCTAAATTGTGTGGCATTAATTACTGCGGTGCCAGAA
GTAAATTCCAAGCGATGTTTCGAAAGCAGTTTTTGCCGCAGAACAAGATCAAAGACAAAT
AATTTTGAAGTTCAAATGAGTACAATTTCATCAAATTCTTATGGGTTGACAGCTGAAATT
AAAAACTTAGAAACTGATGTAATTTTGGTCCTCAAACTTGACTGCTTAGAAAATAATTCA
TTCCATTTTGAAATTGATGAAAAGTCACCATTTAAAAAAAGATTCCGTCTTACAAATCAA
GCAATTGTTGATGACATTAAGAAAAAACCAGCTCAAGTTTCAAATACAACAAATTCAGCG
ACTGTAACATGTGATTTAAATAAAGCAATAATTGAATTTTCACCATTTAAAATTGAATTT
TATCACAATAATGTTTTAAGCTTAATTGTGAATAACAGAAAATTAATGACATTTGAACAT
TTGAGGAATAAAACAGTTCCAACAACAGATGACGATGAATGGGAAGAAACGTTTGAAGGC
TATACTGACAGCAAGCCAAATGGACCTGAAGCTATCGCCCTTGACTTTACAATTGACAAT
TCTGAAATTCTTTTTGGCATTCCTGAACATCACGACACATTTTCACTACGAAGGACACTT
TATGGTGAACCATATCGACTTTTTACAACTGATATGACATTTTTTGAACTTGATAGTCGT
ATGGCAAATTATGGTGCAATTCCAGTGATTTATGGACATACAGCTGATGGAAGGACAACT
GGAATATTCTGGCATAATGCAGCTGACACTTATGTTGATATTTATGACACAAAAACTTCA
CATTTTATGTCAGAAAGTGGCATAATTGATGTTTATATTTTCCTTGGACCATCACCAAGT
GAAACTTTTAAACAATATACACAGATAACAGGCGTTGGAAATTTACCACAACTCTTCACA
CTCGCTTATCATCAATGTCGTTGGAGTTACATGACACAAAATGAAGTGCTTGAAATTGTC
AAGAGTTTTGATCAATATGAAATTCCACTTGACACAATGTGGCTTGATATTGATTATACT
GATGGAAAAAGATATTTTACATGGAATCATACTGCATTTCCAAAGCCATTAGAAATGATT
GCTGAATTAGATTCAACTGGAAGACATTTAGTTTTTATTATTGATCCACATGTTAAAGTT
GATGATGAATATTTCTTTTATGATGGACTAAAAGATTTAAATTATTTTGTTAAACTTGCT
GATACAAAAACTGATTTCACTGGAAATTGTTGGCCTGGTAATTCAAGTTATGTCGATTTT
CTTAATCCTGAAGCTAGAAAATTTTATGCTGATCAATATTTAATAACAAATTTTAATGAA
AGTGTTGTAAAAGGTGGAATATGGAATGATATGAATGAACCAGCTGTTTTTGATGCACCT
GAAAAAACTTTTCCAAGAGATGCAATGCATTATGATGGTGAAAACTTTTGGGAGCATCGA
AATTTGCACAACATGTATGGATTGTTGCATACAACAGGAACATTTGATGGTTTAATGAGA
AGAGGAAGTGGTGACTATCGTCCATTTATTCTTACAAGATCTTTCTTTGCTGGTTCACAA
AAATATACAGCTGTATGGACTGGTGACAACACAGCAGATTGGGGTTATTTGCATGGCTCA
ATTCAAGCTTGTCTTGCAATTTCTGTTGCTGGAATTTCATTTTGTGGTTCTGACATTGGT
GGATTCTTTGGAAATCCTGATGAAGAACTTTTTTATCGATGGACACAAGCTGCAGCATTT
CAACCATTCTTTAGAAATCATGCAAGTGCTGGTACTGAATATCGTGAGCCATATCGATTT
TCTAATGAAACAAGAATTATTGCAACTAATGCAATCAAATTGCGATATTCTTTCTTGCCA
TTCTGGTATACACTTTTTTATGAGCATGAAATAACAGGATTACCAGTCATGCGACCAATG
TTGGCCAATTATCCACTTGATAAAAATGTTTTTAAACTTGATACACAATATATGTTGAGT
GATAAACTTCTTGTTGCTCCAGTGCTTGAACCATTATCATCATGGGTTATTGTAAGATTT
CCATCAATTGATGGCAAAGATAAAGGTGAAATTTGGTATGATATTGATAATTATACAAAA
TATACTCACGTTGGAACTCAAGCTATAAAAGTTGATGAAAATAAAATTGCAGTTTTCCAA
AGAGGTGGAACAATTATAAGTAGAAAAGCAACTGTTAAAAAATCATCAATTTACATGCGT
GGTGATCCAATTTCAATTTATATTGCTGTTGATAAAGATGGAAAAGCTCAAGGAAATGTT
TATTTTGATGATGAAACAAGTTTTGGTTATCGAAATGGAGAATTTAAATTGATTAATTTC
AATTTTGAAAATAAGACTTTAACAGTAACTGAAAATAATTTTAAATCAGATAAAATTCAT
TCAAGTAACATTGAACGAGTTTACATTGCTGGAATTAAAGAATTAAAAACTGCAAAACAA
TCATGCAAGGACAATAATAAAAGAGATTTTTCAGTTGTTGAAATTCATGAAGAATTTTTC
TATATTGATGTCAGTGGAAATGAAGAAAAATGTGAAAATTCTTGGACGTTGACACTGAAT
GGAAGTAATAAGAATTTTATATCAATTGGATTGTTGATGAGTGTTTTAACTTTTGTATTA
TTTAGAATTTTTTTTGAATGA
>g1425.t1 Gene=g1425 Length=906
MKLKLLLFLNCVALITAVPEVNSKRCFESSFCRRTRSKTNNFEVQMSTISSNSYGLTAEI
KNLETDVILVLKLDCLENNSFHFEIDEKSPFKKRFRLTNQAIVDDIKKKPAQVSNTTNSA
TVTCDLNKAIIEFSPFKIEFYHNNVLSLIVNNRKLMTFEHLRNKTVPTTDDDEWEETFEG
YTDSKPNGPEAIALDFTIDNSEILFGIPEHHDTFSLRRTLYGEPYRLFTTDMTFFELDSR
MANYGAIPVIYGHTADGRTTGIFWHNAADTYVDIYDTKTSHFMSESGIIDVYIFLGPSPS
ETFKQYTQITGVGNLPQLFTLAYHQCRWSYMTQNEVLEIVKSFDQYEIPLDTMWLDIDYT
DGKRYFTWNHTAFPKPLEMIAELDSTGRHLVFIIDPHVKVDDEYFFYDGLKDLNYFVKLA
DTKTDFTGNCWPGNSSYVDFLNPEARKFYADQYLITNFNESVVKGGIWNDMNEPAVFDAP
EKTFPRDAMHYDGENFWEHRNLHNMYGLLHTTGTFDGLMRRGSGDYRPFILTRSFFAGSQ
KYTAVWTGDNTADWGYLHGSIQACLAISVAGISFCGSDIGGFFGNPDEELFYRWTQAAAF
QPFFRNHASAGTEYREPYRFSNETRIIATNAIKLRYSFLPFWYTLFYEHEITGLPVMRPM
LANYPLDKNVFKLDTQYMLSDKLLVAPVLEPLSSWVIVRFPSIDGKDKGEIWYDIDNYTK
YTHVGTQAIKVDENKIAVFQRGGTIISRKATVKKSSIYMRGDPISIYIAVDKDGKAQGNV
YFDDETSFGYRNGEFKLINFNFENKTLTVTENNFKSDKIHSSNIERVYIAGIKELKTAKQ
SCKDNNKRDFSVVEIHEEFFYIDVSGNEEKCENSWTLTLNGSNKNFISIGLLMSVLTFVL
FRIFFE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 22 | g1425.t1 | CDD | cd14752 | GH31_N | 192 | 311 | 2.47385E-26 |
| 23 | g1425.t1 | CDD | cd06603 | GH31_GANC_GANAB_alpha | 311 | 787 | 0.0 |
| 10 | g1425.t1 | Gene3D | G3DSA:2.60.40.1760 | glycosyl hydrolase (family 31) | 40 | 311 | 1.2E-37 |
| 13 | g1425.t1 | Gene3D | G3DSA:3.20.20.80 | Glycosidases | 313 | 488 | 2.1E-58 |
| 14 | g1425.t1 | Gene3D | G3DSA:3.20.20.80 | Glycosidases | 489 | 663 | 2.2E-65 |
| 12 | g1425.t1 | Gene3D | G3DSA:2.60.40.1180 | - | 664 | 742 | 4.2E-11 |
| 11 | g1425.t1 | Gene3D | G3DSA:2.60.40.1180 | - | 743 | 887 | 1.7E-10 |
| 3 | g1425.t1 | PANTHER | PTHR22762 | ALPHA-GLUCOSIDASE | 9 | 853 | 2.3E-266 |
| 4 | g1425.t1 | PANTHER | PTHR22762:SF54 | BCDNA.GH04962 | 9 | 853 | 2.3E-266 |
| 1 | g1425.t1 | Pfam | PF13802 | Galactose mutarotase-like | 202 | 273 | 3.3E-17 |
| 2 | g1425.t1 | Pfam | PF01055 | Glycosyl hydrolases family 31 | 292 | 746 | 4.9E-137 |
| 17 | g1425.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 23 | - |
| 18 | g1425.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 19 | g1425.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 15 | - |
| 21 | g1425.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 23 | - |
| 16 | g1425.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 24 | 885 | - |
| 20 | g1425.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 886 | 905 | - |
| 15 | g1425.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 906 | 906 | - |
| 25 | g1425.t1 | ProSitePatterns | PS00129 | Glycosyl hydrolases family 31 active site. | 466 | 473 | - |
| 6 | g1425.t1 | SUPERFAMILY | SSF74650 | Galactose mutarotase-like | 44 | 299 | 3.45E-18 |
| 7 | g1425.t1 | SUPERFAMILY | SSF51445 | (Trans)glycosidases | 302 | 653 | 1.56E-74 |
| 5 | g1425.t1 | SUPERFAMILY | SSF51011 | Glycosyl hydrolase domain | 655 | 742 | 1.37E-12 |
| 9 | g1425.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 17 | - |
| 8 | g1425.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 14 | - |
| 24 | g1425.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 886 | 905 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0030246 | carbohydrate binding | MF |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds | MF |
| GO:0005975 | carbohydrate metabolic process | BP |
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
- This transcript has been annotated with K12317 (neutral alpha-glucosidase C)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed