General information for g14250.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein.
Parent gene
- The parent gene g14250 is located on chr_1: 35839462..35840788 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g14250 | g14250.t1 | isoform | g14250.t1 | 35839388 | 35840788 |
| chr_1 | g14250 | g14250.t1 | exon | g14250.t1.exon1 | 35839388 | 35839398 |
| chr_1 | g14250 | g14250.t1 | cds | g14250.t1.CDS1 | 35839388 | 35839398 |
| chr_1 | g14250 | g14250.t1 | exon | g14250.t1.exon2 | 35839462 | 35840788 |
| chr_1 | g14250 | g14250.t1 | cds | g14250.t1.CDS2 | 35839462 | 35840788 |
| chr_1 | g14250 | g14250.t1 | TTS | g14250.t1 | 35840896 | 35840896 |
| chr_1 | g14250 | g14250.t1 | TSS | g14250.t1 | NA | NA |
Sequences
>g14250.t1 Gene=g14250 Length=1338
ATGAATATTTGTTTAGTATCAGATTTTTTCTATCCAAATCATGGTGGAGTGGAAGAACAT
ATTTATAATTTATCACAATGTCTTTTATCAAGCGGTCATAAAGTTATAATAATTACTCAT
TCTTATGGCAATCGTAAAGGAATTCGATATATGACAAATGGTCTCAAAGTTTATTATCTT
CCAATTAAAGTGTTTTATAATCAATGTATCCTCCCAACAATGATTTGTAACATAAAAATT
CTCAGAGAAATTTTTATACGAGAACAAATACAAATAATCCACGGACATTCTGCATTCTCT
GTGCTGGCACATGAAGCAATGACAATTGGAGTTTTGATGGGACGAAGAGCAATATTTACA
GATCACAGCCTTTTTGGATTTGCAGATCTCAGTGCGATTTTGACAAACAAATTCCTTCAA
ATAAGTCTCTCATTCTGCAATCATTTTATTTGTGTGTCACATACCGGCAAAGAGAATACA
GTTCTTCGGGCAAAAATGAAAGCCTCAAAAGTTTCTGTAATTCCAAATGCTGTCAACACT
TTACAATTTACGCCTAATCCAATAAGCCGTGATAAGAAAATGATAACAATTGTGATTGTG
TCAAGATTAGTTTATCGTAAAGGAATTGATTTACTAGCTTCAATCATTCCATATTTTAAA
AATAGGCATTATATAAATTTTATAATTGCTGGTGATGGACCAAAAAGAGAGCTATTGGAA
GAAGTTCGTGAAAAGAATAATATGCAAGACAGAATTCAAATGTTGGGTGCCCTCGAACAT
TCTGAAGTTAGAGATGTGCTGACAAGAGGTCATATTTTTTTAAACACATCACTCACTGAA
GCTTTCTGTATGACAATAGTTGAAGCCTGTTCAGTTGGTTTGCATGTAATTTCTACAAGA
GTTGGTGGTGTACCAGAAGTTCTGCCAGAATATTTAAATGTGATTCTTGTTCAACCTGAT
ATAAAATCATTAATAAATGCTCTTGAAGAAACAATCGATAGAGTTGAAAGGTTTAGAGCA
GGAAAATCAAGAAAAGATGAAATAATATGTCCTTTCAAAATCAATCAAATCGTATCAGAA
CTATACAATTGGCAGGATGTTAGTCATAGAACTGAGAAAATTTATCGCAAGGTTCTCGAA
GAACCAACAAAAAATTTGAAGGAAGTTATGTTAACAATTGTAAATAGGAGTGGAGTTTAT
CTATTTTTACTTGTCGTTTCACTTTGTTATGTAATCATAAAATTCTATGATTGGCTTTAT
CCAGCAAATGAAATTGAAATATGTCGAAATTACAACGAAATGAAGAATAAGAATAAAAAC
AACTACCAAAGAAGTTGA
>g14250.t1 Gene=g14250 Length=445
MNICLVSDFFYPNHGGVEEHIYNLSQCLLSSGHKVIIITHSYGNRKGIRYMTNGLKVYYL
PIKVFYNQCILPTMICNIKILREIFIREQIQIIHGHSAFSVLAHEAMTIGVLMGRRAIFT
DHSLFGFADLSAILTNKFLQISLSFCNHFICVSHTGKENTVLRAKMKASKVSVIPNAVNT
LQFTPNPISRDKKMITIVIVSRLVYRKGIDLLASIIPYFKNRHYINFIIAGDGPKRELLE
EVREKNNMQDRIQMLGALEHSEVRDVLTRGHIFLNTSLTEAFCMTIVEACSVGLHVISTR
VGGVPEVLPEYLNVILVQPDIKSLINALEETIDRVERFRAGKSRKDEIICPFKINQIVSE
LYNWQDVSHRTEKIYRKVLEEPTKNLKEVMLTIVNRSGVYLFLLVVSLCYVIIKFYDWLY
PANEIEICRNYNEMKNKNKNNYQRS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g14250.t1 | CDD | cd03796 | GT4_PIG-A-like | 2 | 400 | 0.0 |
| 6 | g14250.t1 | Gene3D | G3DSA:3.40.50.2000 | Glycogen Phosphorylase B; | 1 | 198 | 2.9E-35 |
| 7 | g14250.t1 | Gene3D | G3DSA:3.40.50.2000 | Glycogen Phosphorylase B; | 199 | 358 | 1.9E-28 |
| 3 | g14250.t1 | PANTHER | PTHR45871 | N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN | 1 | 439 | 1.9E-176 |
| 2 | g14250.t1 | Pfam | PF08288 | PIGA (GPI anchor biosynthesis) | 40 | 129 | 2.4E-40 |
| 1 | g14250.t1 | Pfam | PF00534 | Glycosyl transferases group 1 | 190 | 333 | 1.0E-24 |
| 9 | g14250.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 398 | - |
| 10 | g14250.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 399 | 419 | - |
| 8 | g14250.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 420 | 445 | - |
| 5 | g14250.t1 | SUPERFAMILY | SSF53756 | UDP-Glycosyltransferase/glycogen phosphorylase | 1 | 379 | 6.63E-82 |
| 4 | g14250.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 398 | 420 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006506 | GPI anchor biosynthetic process | BP |
| GO:0017176 | phosphatidylinositol N-acetylglucosaminyltransferase activity | MF |
| GO:0016757 | glycosyltransferase activity | MF |
| GO:0000506 | glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex | CC |
KEGG
Orthology
- This transcript has been annotated with K03857 (phosphatidylinositol N-acetylglucosaminyltransferase subunit A)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
