General information for g14250.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein.
Parent gene
- The parent gene g14250 is located on chr_1: 35839462..35840788 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g14250 | g14250.t2 | isoform | g14250.t2 | 35839462 | 35840788 |
| chr_1 | g14250 | g14250.t2 | exon | g14250.t2.exon1 | 35839462 | 35840788 |
| chr_1 | g14250 | g14250.t2 | cds | g14250.t2.CDS1 | 35839601 | 35840788 |
| chr_1 | g14250 | g14250.t2 | TTS | g14250.t2 | 35840896 | 35840896 |
| chr_1 | g14250 | g14250.t2 | TSS | g14250.t2 | NA | NA |
Sequences
>g14250.t2 Gene=g14250 Length=1327
TTTAGTATCAGATTTTTTCTATCCAAATCATGGTGGAGTGGAAGAACATATTTATAATTT
ATCACAATGTCTTTTATCAAGCGGTCATAAAGTTATAATAATTACTCATTCTTATGGCAA
TCGTAAAGGAATTCGATATATGACAAATGGTCTCAAAGTTTATTATCTTCCAATTAAAGT
GTTTTATAATCAATGTATCCTCCCAACAATGATTTGTAACATAAAAATTCTCAGAGAAAT
TTTTATACGAGAACAAATACAAATAATCCACGGACATTCTGCATTCTCTGTGCTGGCACA
TGAAGCAATGACAATTGGAGTTTTGATGGGACGAAGAGCAATATTTACAGATCACAGCCT
TTTTGGATTTGCAGATCTCAGTGCGATTTTGACAAACAAATTCCTTCAAATAAGTCTCTC
ATTCTGCAATCATTTTATTTGTGTGTCACATACCGGCAAAGAGAATACAGTTCTTCGGGC
AAAAATGAAAGCCTCAAAAGTTTCTGTAATTCCAAATGCTGTCAACACTTTACAATTTAC
GCCTAATCCAATAAGCCGTGATAAGAAAATGATAACAATTGTGATTGTGTCAAGATTAGT
TTATCGTAAAGGAATTGATTTACTAGCTTCAATCATTCCATATTTTAAAAATAGGCATTA
TATAAATTTTATAATTGCTGGTGATGGACCAAAAAGAGAGCTATTGGAAGAAGTTCGTGA
AAAGAATAATATGCAAGACAGAATTCAAATGTTGGGTGCCCTCGAACATTCTGAAGTTAG
AGATGTGCTGACAAGAGGTCATATTTTTTTAAACACATCACTCACTGAAGCTTTCTGTAT
GACAATAGTTGAAGCCTGTTCAGTTGGTTTGCATGTAATTTCTACAAGAGTTGGTGGTGT
ACCAGAAGTTCTGCCAGAATATTTAAATGTGATTCTTGTTCAACCTGATATAAAATCATT
AATAAATGCTCTTGAAGAAACAATCGATAGAGTTGAAAGGTTTAGAGCAGGAAAATCAAG
AAAAGATGAAATAATATGTCCTTTCAAAATCAATCAAATCGTATCAGAACTATACAATTG
GCAGGATGTTAGTCATAGAACTGAGAAAATTTATCGCAAGGTTCTCGAAGAACCAACAAA
AAATTTGAAGGAAGTTATGTTAACAATTGTAAATAGGAGTGGAGTTTATCTATTTTTACT
TGTCGTTTCACTTTGTTATGTAATCATAAAATTCTATGATTGGCTTTATCCAGCAAATGA
AATTGAAATATGTCGAAATTACAACGAAATGAAGAATAAGAATAAAAACAACTACCAAAG
AAGTTGA
>g14250.t2 Gene=g14250 Length=395
MTNGLKVYYLPIKVFYNQCILPTMICNIKILREIFIREQIQIIHGHSAFSVLAHEAMTIG
VLMGRRAIFTDHSLFGFADLSAILTNKFLQISLSFCNHFICVSHTGKENTVLRAKMKASK
VSVIPNAVNTLQFTPNPISRDKKMITIVIVSRLVYRKGIDLLASIIPYFKNRHYINFIIA
GDGPKRELLEEVREKNNMQDRIQMLGALEHSEVRDVLTRGHIFLNTSLTEAFCMTIVEAC
SVGLHVISTRVGGVPEVLPEYLNVILVQPDIKSLINALEETIDRVERFRAGKSRKDEIIC
PFKINQIVSELYNWQDVSHRTEKIYRKVLEEPTKNLKEVMLTIVNRSGVYLFLLVVSLCY
VIIKFYDWLYPANEIEICRNYNEMKNKNKNNYQRS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g14250.t2 | Gene3D | G3DSA:3.40.50.2000 | Glycogen Phosphorylase B; | 20 | 311 | 3.2E-51 |
| 6 | g14250.t2 | Gene3D | G3DSA:3.40.50.2000 | Glycogen Phosphorylase B; | 128 | 302 | 3.2E-51 |
| 3 | g14250.t2 | PANTHER | PTHR45871 | N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN | 1 | 389 | 1.4E-145 |
| 2 | g14250.t2 | Pfam | PF08288 | PIGA (GPI anchor biosynthesis) | 1 | 79 | 1.6E-33 |
| 1 | g14250.t2 | Pfam | PF00534 | Glycosyl transferases group 1 | 140 | 283 | 8.0E-25 |
| 8 | g14250.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 342 | - |
| 10 | g14250.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 343 | 363 | - |
| 9 | g14250.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 364 | 395 | - |
| 5 | g14250.t2 | SUPERFAMILY | SSF53756 | UDP-Glycosyltransferase/glycogen phosphorylase | 3 | 329 | 5.1E-66 |
| 4 | g14250.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 348 | 370 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006506 | GPI anchor biosynthetic process | BP |
| GO:0016757 | glycosyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K03857 (phosphatidylinositol N-acetylglucosaminyltransferase subunit A)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
