General information for g14252.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative fatty acyl-CoA reductase CG5065.
Parent gene
- The parent gene g14252 is located on chr_1: 35858266..35860728 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g14252 | g14252.t1 | TSS | g14252.t1 | 35858236 | 35858236 |
| chr_1 | g14252 | g14252.t1 | isoform | g14252.t1 | 35858266 | 35860728 |
| chr_1 | g14252 | g14252.t1 | exon | g14252.t1.exon1 | 35858266 | 35858335 |
| chr_1 | g14252 | g14252.t1 | cds | g14252.t1.CDS1 | 35858266 | 35858335 |
| chr_1 | g14252 | g14252.t1 | exon | g14252.t1.exon2 | 35858423 | 35858553 |
| chr_1 | g14252 | g14252.t1 | cds | g14252.t1.CDS2 | 35858423 | 35858553 |
| chr_1 | g14252 | g14252.t1 | exon | g14252.t1.exon3 | 35858609 | 35859032 |
| chr_1 | g14252 | g14252.t1 | cds | g14252.t1.CDS3 | 35858609 | 35859032 |
| chr_1 | g14252 | g14252.t1 | exon | g14252.t1.exon4 | 35859116 | 35859235 |
| chr_1 | g14252 | g14252.t1 | cds | g14252.t1.CDS4 | 35859116 | 35859235 |
| chr_1 | g14252 | g14252.t1 | exon | g14252.t1.exon5 | 35859298 | 35859314 |
| chr_1 | g14252 | g14252.t1 | cds | g14252.t1.CDS5 | 35859298 | 35859314 |
| chr_1 | g14252 | g14252.t1 | exon | g14252.t1.exon6 | 35859373 | 35859521 |
| chr_1 | g14252 | g14252.t1 | cds | g14252.t1.CDS6 | 35859373 | 35859521 |
| chr_1 | g14252 | g14252.t1 | exon | g14252.t1.exon7 | 35859953 | 35860192 |
| chr_1 | g14252 | g14252.t1 | cds | g14252.t1.CDS7 | 35859953 | 35860192 |
| chr_1 | g14252 | g14252.t1 | exon | g14252.t1.exon8 | 35860256 | 35860513 |
| chr_1 | g14252 | g14252.t1 | cds | g14252.t1.CDS8 | 35860256 | 35860513 |
| chr_1 | g14252 | g14252.t1 | exon | g14252.t1.exon9 | 35860632 | 35860728 |
| chr_1 | g14252 | g14252.t1 | cds | g14252.t1.CDS9 | 35860632 | 35860728 |
| chr_1 | g14252 | g14252.t1 | TTS | g14252.t1 | NA | NA |
Sequences
>g14252.t1 Gene=g14252 Length=1506
ATGTCACAAAAACTTAATTCAGTTCAAGAGTTCTATAAGAACAAGACCATCTTCATTACT
GGAGGATCTGGATTTATGGGAAAAGTTCTCATTGAGAAGCTTTTGTATTCATGTTCTGAT
GTTAAAGAACTAATAATTTTAATGCGACCAAAAAAAGGAAAAACCGCACAAGAACGTGTC
GAAGATTTTGCAAAGTTACCAATGTTCAAAAGATTAATGGATGAGAAACCAGAAATGTTG
AAAAAAATTTATCCCGTTTGGGGGGAAATTACGCAAGAAAATTTAGGTCTAAATGACGAA
CATTTATTGCGCGTTATCAACTCAACACAACTTATTTTTCATCTTGCCGCATCACTTAAG
CTTGAAGCTCCACTCAAGCCAAACGTCATCATGAATTTAACCGGAACAAAATATGTCTTA
GATGTTGCTAAGAAAATGAAAAATCTTATTTACATGATTCATACTTCAACTGCATTCTGT
AATGTTGAATATGAAACGCTTGAGGAGAAAGTTTATGATTTTCCTCATAAACCTTTGGAC
CTGATAAGATCAGCTGAATGGATGACAGACGAAGCAATGACATTGTCACAAAAAGACACA
CTTGGAGTTCATCCCAATACTTATACTTATACAAAACGTCTTGCTGAAATCCTTGTTCGA
GATGAATATGTTGAGAATAATTTACCGATATGCATTGTTAGACCTAGCATTGTGACACCT
GCATTAAATGAACCTGTTCCAGGATGGGTAGACTCTCTTAATGGATCGCCGGGAATTATT
GCAGCTGTAGGCAAAGGAGCATTACGTTGTATGTTGTTAAATACAGAAGCTAATTTTGAA
GCTATCCCAGTTGATTTAGCAATAAACGGACTTTTAATGATTATGAAGAAACTTGCTACT
CAATTTGAAAGGTCAAATGAAATTCCAGTTTTTAATGTTACAAATCACAAAGATGTGAGA
ATAACATATGGAGAATTTTTCAATCTTTGTAATGCAATGAAATTTGAAACTCCTTGTTCT
ATCTCTCTTTGGTATCCTAATGCAACACTAACATTGAATAAATTCTATTATTATTTCAAC
GTTTTGCTTTTTCAATGGATTCCTGCATTATTCATAGACTTTCTTTTGTTGCTTTTTGGT
CAAAAGAGATTTATGATACACGTTCAAAAAAGAATTGCAATCGGCATGGATGTATTGGCT
TGTTTTACGATGAATAATTGGAATTTCAAGTCGAAAAACTTTGAAGATTTAATAAAAATT
CAATCAAAAGAAGAATATGAAATGTTCTTCATTGATTCAAAGAAGATTGATGTTAAATCA
GCATCGAGACAAGCAATCATTGGTGGGAGAGTTTATTGTCTCAAAGATCCCATGTCGACA
ATTCCAAAAGCAAGAAAATTAATTTGGATTCAATATGTAATCGATCGAGTTATTAAAATA
TTTTTCTTTTATTGGTTAATTAAGAAAGTTTTAACTTATACTGGACTTATGGACCGATTT
TATTGA
>g14252.t1 Gene=g14252 Length=501
MSQKLNSVQEFYKNKTIFITGGSGFMGKVLIEKLLYSCSDVKELIILMRPKKGKTAQERV
EDFAKLPMFKRLMDEKPEMLKKIYPVWGEITQENLGLNDEHLLRVINSTQLIFHLAASLK
LEAPLKPNVIMNLTGTKYVLDVAKKMKNLIYMIHTSTAFCNVEYETLEEKVYDFPHKPLD
LIRSAEWMTDEAMTLSQKDTLGVHPNTYTYTKRLAEILVRDEYVENNLPICIVRPSIVTP
ALNEPVPGWVDSLNGSPGIIAAVGKGALRCMLLNTEANFEAIPVDLAINGLLMIMKKLAT
QFERSNEIPVFNVTNHKDVRITYGEFFNLCNAMKFETPCSISLWYPNATLTLNKFYYYFN
VLLFQWIPALFIDFLLLLFGQKRFMIHVQKRIAIGMDVLACFTMNNWNFKSKNFEDLIKI
QSKEEYEMFFIDSKKIDVKSASRQAIIGGRVYCLKDPMSTIPKARKLIWIQYVIDRVIKI
FFFYWLIKKVLTYTGLMDRFY
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g14252.t1 | CDD | cd05236 | FAR-N_SDR_e | 15 | 327 | 8.1329E-106 |
| 15 | g14252.t1 | CDD | cd09071 | FAR_C | 363 | 454 | 3.21004E-16 |
| 8 | g14252.t1 | Gene3D | G3DSA:3.40.50.720 | - | 2 | 352 | 1.3E-65 |
| 3 | g14252.t1 | PANTHER | PTHR11011 | MALE STERILITY PROTEIN 2-RELATED | 3 | 492 | 5.1E-188 |
| 4 | g14252.t1 | PANTHER | PTHR11011:SF12 | FATTY ACYL-COA REDUCTASE | 3 | 492 | 5.1E-188 |
| 1 | g14252.t1 | Pfam | PF07993 | Male sterility protein | 19 | 289 | 2.3E-66 |
| 2 | g14252.t1 | Pfam | PF03015 | Male sterility protein | 364 | 456 | 8.5E-23 |
| 11 | g14252.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 354 | - |
| 13 | g14252.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 355 | 380 | - |
| 9 | g14252.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 381 | 466 | - |
| 12 | g14252.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 467 | 487 | - |
| 10 | g14252.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 488 | 501 | - |
| 7 | g14252.t1 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 7 | 287 | 4.24E-26 |
| 5 | g14252.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 358 | 380 | - |
| 6 | g14252.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 468 | 487 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0080019 | fatty-acyl-CoA reductase (alcohol-forming) activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
