General information for g14263.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Glutamine-dependent NAD(+) synthetase.
Parent gene
- The parent gene g14263 is located on chr_1: 35996948..35999523 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g14263 | g14263.t1 | isoform | g14263.t1 | 35996948 | 35999523 |
| chr_1 | g14263 | g14263.t1 | exon | g14263.t1.exon1 | 35996948 | 35997093 |
| chr_1 | g14263 | g14263.t1 | cds | g14263.t1.CDS1 | 35996948 | 35997093 |
| chr_1 | g14263 | g14263.t1 | exon | g14263.t1.exon2 | 35997153 | 35997367 |
| chr_1 | g14263 | g14263.t1 | cds | g14263.t1.CDS2 | 35997153 | 35997367 |
| chr_1 | g14263 | g14263.t1 | exon | g14263.t1.exon3 | 35997420 | 35998100 |
| chr_1 | g14263 | g14263.t1 | cds | g14263.t1.CDS3 | 35997420 | 35998100 |
| chr_1 | g14263 | g14263.t1 | exon | g14263.t1.exon4 | 35998166 | 35999523 |
| chr_1 | g14263 | g14263.t1 | cds | g14263.t1.CDS4 | 35998166 | 35999523 |
| chr_1 | g14263 | g14263.t1 | TSS | g14263.t1 | NA | NA |
| chr_1 | g14263 | g14263.t1 | TTS | g14263.t1 | NA | NA |
Sequences
>g14263.t1 Gene=g14263 Length=2400
ATGGGTCGAAAGGTGACCGTTGCGGCCTGTACCTTAAACCAATGGGCGCTGGATTTTGAG
GGAAATATGGCAAGAATCATGCAGTCGATTTTAGAAGCACGCGAACTTGGAGGCGTTTAT
CGAACTGGTCCAGAATTGGAAATTCCTGGTTACAGCTGTGAAGATCATTTTTTTGAGTCG
GACACATTTTTACATTCATGGGAAGTGCTTTTACAAATTATGATGTCACCTATTTGTCAG
AATATGCTAATTGATGTTGGCATGCCCGTTCAACATTTCAACGTTGCTTATAATTGTCGT
GTGATTTTTTATAATAAAAAAATTATTCTCATAAGACCAAAAATGATGATGTGTGATGAT
GGCAATTATCGTGAATCTCGATGGTTTTCAGCATGGACAAAAATAAGAAATATTGAAGAT
TATTATCTTCCAAGAATTGTAGCAACGGCTACAGGCCAACATACTGTTCCATTTGGTGAT
GCAGTGATTTCAACTAGGGAAACATGCATAGGTTTCGAAATATGTGAAGAGTTATGGAAT
CCACGTTCAACACATATCGATTTAAGTTTATCAGGTGTTGAAATTATGGTTAATGGTAGT
GGCAGTTATATTCAATTACGTAAATCCCATATCACCACTGACTTAATAAGGAATGCTTCA
TATAAAGCAGGTGGTGCTTATATTTTTTCCAACTTGCGTGGATGTGATGGACAACGAGTT
TGGTGGGGCGGAAATTCAGCAATTGCTCTTAATGGTGAAATAATTGCAAGAAGTAAGCAA
TTTTCTTTAACAGATGTTGAAGTGACAGTAGCAACTATTGATTTAGAAGATATCAGAAGT
TATCGAATGGCTTTAAGATCACGCTGCACACTTGGTGCGTCTGCTCCACTTTATCCAAGA
ATTTTTGTGGACTGTGAACTATCTAACCGTGGTGACATATTTATTGCTGCTCATCCACCA
ATTCAATGGACATATCATACTCCAGAAGAAGAAATTGCTTTTGCGCCTGCGTGCTGGCTA
TGGGATTATTTACGTCGATCAGGTCAAGGAGGATTTTTCTTGCCATTATCCGGCGGTATT
GATAGTAGCAGCAGTGCTTGTATAATCCACAGTATGTGTCGAATGGTTGTTGATGCAATT
CAATATGGTGAAGCACAAGTTTTACATGATGTAAGAAAAATTCTTGCTGATCCTGATTTC
ACACCTCATAATGCTGCACAATTATGCAATAGATTGCTTGTAACTTGTTTTATGGGATCA
GAAAATTCAAGTCACGAAACACGTCGTCGTGCATCAGTTTTAGCAAATCAAATTGGAAGT
TATCATTTAGAAATTAATATTGATAGCACTGTGAACACAATGCTATCAATTTTCAATGCG
GTTACTGGTTTTTCACCGAGATTTTTCATTCAAGGCGGATGCCCAAGACAAAATCTTGCA
TTGCAAAATATTCAAGCACGTGTTCGTATGGTTTTGTCTTATCTATTTGCACAATTGATG
TTGTGGACAAGAAATCGTCCAGGCGGTCTTTTAGTGCTTGGTTCAGCAAACGTTGATGAA
TCATTGCGTGGCTATATGACAAAATATGATTGCTCATCAGCTGATTGCAATCCAATAGGT
GGAATTTCAAAAACTGATTTGCGAAAATTTTTGAATTATGCGATGGAAAAATTTAACATT
CCAATATTAAGTGAAATAATTTCAGCTCCGCCAACAGCTGAACTTGAGCCACTTCATGAA
GGTCAATTAGCACAAACAGATGAGCAAGATATGGGTATGACATATAGTGAATTGTGTGAA
TTTGGAAGATTAAGAAAACAAGCGGCCTGTGGTCCTTATTCCATGTTCTGTAAATTGATT
GCAAGTTGGCGTAATGAATGTGCTCCAAAAGAAGTTAGTGACAAAGTCAAGCATTTCTTT
CGCTGCTACGCAATTAATCGCCATAAAATGACTGTTTTAACTCCTTCTGTACACATGGAC
AGCTACTCACCTGATGACAATAGATTTGACCATCGACCTTTTCTTTATAGAGCCAATTGG
AGTTGGCAATTTAAGGCAATCGATGATGAATTGGATAGAATTACACGAGCTTCAAACATA
GCACAGAAAAGTGATAAAAGTGAACATGAAAATGGAGAGTTTAAGACACGTAGCGAAAGT
CATGGAAAAATTGAAAAGCAGCAGAATTCACCGGTTTATTTCGAAGCACATGATGGTCCA
CTTACAAGTGATCATATTATTACAACAAATGGAATGTCAGGTGCTTGTTCAAGCGGAAAT
ATTTTTTTAATTGAAAAATCTCAAGTACAGTTAAAGAAAAGTCATAGTACTGGTTATAAT
AAAAATCAGGGTGGAAATGTTATGGGTCGAATTAAAGACCGGACTGGAGTTGCTGTCTAA
>g14263.t1 Gene=g14263 Length=799
MGRKVTVAACTLNQWALDFEGNMARIMQSILEARELGGVYRTGPELEIPGYSCEDHFFES
DTFLHSWEVLLQIMMSPICQNMLIDVGMPVQHFNVAYNCRVIFYNKKIILIRPKMMMCDD
GNYRESRWFSAWTKIRNIEDYYLPRIVATATGQHTVPFGDAVISTRETCIGFEICEELWN
PRSTHIDLSLSGVEIMVNGSGSYIQLRKSHITTDLIRNASYKAGGAYIFSNLRGCDGQRV
WWGGNSAIALNGEIIARSKQFSLTDVEVTVATIDLEDIRSYRMALRSRCTLGASAPLYPR
IFVDCELSNRGDIFIAAHPPIQWTYHTPEEEIAFAPACWLWDYLRRSGQGGFFLPLSGGI
DSSSSACIIHSMCRMVVDAIQYGEAQVLHDVRKILADPDFTPHNAAQLCNRLLVTCFMGS
ENSSHETRRRASVLANQIGSYHLEINIDSTVNTMLSIFNAVTGFSPRFFIQGGCPRQNLA
LQNIQARVRMVLSYLFAQLMLWTRNRPGGLLVLGSANVDESLRGYMTKYDCSSADCNPIG
GISKTDLRKFLNYAMEKFNIPILSEIISAPPTAELEPLHEGQLAQTDEQDMGMTYSELCE
FGRLRKQAACGPYSMFCKLIASWRNECAPKEVSDKVKHFFRCYAINRHKMTVLTPSVHMD
SYSPDDNRFDHRPFLYRANWSWQFKAIDDELDRITRASNIAQKSDKSEHENGEFKTRSES
HGKIEKQQNSPVYFEAHDGPLTSDHIITTNGMSGACSSGNIFLIEKSQVQLKKSHSTGYN
KNQGGNVMGRIKDRTGVAV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 17 | g14263.t1 | CDD | cd07570 | GAT_Gln-NAD-synth | 6 | 287 | 4.88564E-84 |
| 16 | g14263.t1 | CDD | cd00553 | NAD_synthase | 328 | 650 | 3.17379E-86 |
| 9 | g14263.t1 | Gene3D | G3DSA:3.60.110.10 | - | 2 | 289 | 4.5E-78 |
| 8 | g14263.t1 | Gene3D | G3DSA:3.40.50.620 | HUPs | 328 | 696 | 7.0E-135 |
| 5 | g14263.t1 | Hamap | MF_02090 | Glutamine-dependent NAD(+) synthetase [nadE]. | 5 | 681 | 28.26107 |
| 18 | g14263.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 700 | 725 | - |
| 19 | g14263.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 702 | 724 | - |
| 3 | g14263.t1 | PANTHER | PTHR23090:SF9 | GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE | 1 | 692 | 0.0 |
| 4 | g14263.t1 | PANTHER | PTHR23090 | NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE | 1 | 692 | 0.0 |
| 15 | g14263.t1 | PIRSF | PIRSF006630 | NADS_GAT | 2 | 684 | 1.4E-154 |
| 1 | g14263.t1 | Pfam | PF00795 | Carbon-nitrogen hydrolase | 6 | 282 | 2.9E-42 |
| 2 | g14263.t1 | Pfam | PF02540 | NAD synthase | 339 | 600 | 5.0E-27 |
| 11 | g14263.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 12 | g14263.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 13 | g14263.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 12 | - |
| 14 | g14263.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 16 | - |
| 10 | g14263.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 799 | - |
| 21 | g14263.t1 | ProSiteProfiles | PS50263 | Carbon-nitrogen hydrolase domain profile. | 5 | 275 | 39.943 |
| 7 | g14263.t1 | SUPERFAMILY | SSF56317 | Carbon-nitrogen hydrolase | 3 | 287 | 4.97E-45 |
| 6 | g14263.t1 | SUPERFAMILY | SSF52402 | Adenine nucleotide alpha hydrolases-like | 319 | 662 | 2.27E-63 |
| 20 | g14263.t1 | TIGRFAM | TIGR00552 | nadE: NAD+ synthetase | 339 | 649 | 3.1E-24 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006807 | nitrogen compound metabolic process | BP |
| GO:0005524 | ATP binding | MF |
| GO:0004359 | glutaminase activity | MF |
| GO:0003952 | NAD+ synthase (glutamine-hydrolyzing) activity | MF |
| GO:0005737 | cytoplasm | CC |
| GO:0009435 | NAD biosynthetic process | BP |
KEGG
Orthology
- This transcript has been annotated with K01950 (NAD+ synthase (glutamine-hydrolysing))
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
